Shigella phage Sf21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus; Shigella virus Sf21

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 266 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VMP4|A0A2K9VMP4_9CAUD Uncharacterized protein OS=Shigella phage Sf21 OX=2024308 GN=Sf21_gp72 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.05YY3 pKa = 10.82AIKK6 pKa = 9.76PWWAARR12 pKa = 11.84WEE14 pKa = 4.45TVEE17 pKa = 4.62PEE19 pKa = 4.09PEE21 pKa = 3.97EE22 pKa = 3.69PVYY25 pKa = 10.7TDD27 pKa = 3.78EE28 pKa = 3.95EE29 pKa = 4.62TIYY32 pKa = 11.07NEE34 pKa = 3.65PTINDD39 pKa = 4.85LIDD42 pKa = 3.12MEE44 pKa = 4.59MGHH47 pKa = 7.03DD48 pKa = 3.76YY49 pKa = 11.32SRR51 pKa = 4.75

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VND4|A0A2K9VND4_9CAUD RIIB protein OS=Shigella phage Sf21 OX=2024308 GN=Sf21_gp266 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.79YY3 pKa = 7.56MTATDD8 pKa = 4.54LNNAGATVIGTIKK21 pKa = 10.4NGEE24 pKa = 4.07WFLGTPHH31 pKa = 7.51KK32 pKa = 11.0DD33 pKa = 3.38FLSKK37 pKa = 10.45PGFYY41 pKa = 10.64FLVSKK46 pKa = 11.06LDD48 pKa = 3.42GRR50 pKa = 11.84PFSNPCVSARR60 pKa = 11.84FYY62 pKa = 11.36VGNQRR67 pKa = 11.84SKK69 pKa = 10.58QGFSAVLSHH78 pKa = 6.11IRR80 pKa = 11.84QRR82 pKa = 11.84RR83 pKa = 11.84SQLARR88 pKa = 11.84TIANNNVPYY97 pKa = 10.75AVFYY101 pKa = 11.17LPASKK106 pKa = 9.84MKK108 pKa = 10.21PLTTGFGKK116 pKa = 10.64GQLALAFTRR125 pKa = 11.84NHH127 pKa = 5.74HH128 pKa = 6.17SEE130 pKa = 4.15YY131 pKa = 9.53QTLEE135 pKa = 3.57EE136 pKa = 4.44MNRR139 pKa = 11.84MLADD143 pKa = 3.15NFKK146 pKa = 10.83FVLQAYY152 pKa = 9.08

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

266

0

266

51969

32

1289

195.4

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.361 ± 0.173

1.105 ± 0.079

6.321 ± 0.102

7.354 ± 0.16

4.505 ± 0.108

5.977 ± 0.204

1.672 ± 0.074

7.558 ± 0.137

7.893 ± 0.21

7.366 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.094

5.605 ± 0.123

3.429 ± 0.094

3.379 ± 0.093

4.156 ± 0.104

6.642 ± 0.156

5.663 ± 0.218

6.531 ± 0.122

1.409 ± 0.056

4.353 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski