Amino acid dipepetide frequency for Paenibacillus sp. YN15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.292AlaAla: 12.292 ± 0.148
0.83AlaCys: 0.83 ± 0.02
5.104AlaAsp: 5.104 ± 0.062
6.608AlaGlu: 6.608 ± 0.067
3.462AlaPhe: 3.462 ± 0.042
8.705AlaGly: 8.705 ± 0.1
1.383AlaHis: 1.383 ± 0.025
4.777AlaIle: 4.777 ± 0.052
4.217AlaLys: 4.217 ± 0.054
8.947AlaLeu: 8.947 ± 0.075
2.355AlaMet: 2.355 ± 0.04
2.846AlaAsn: 2.846 ± 0.04
3.564AlaPro: 3.564 ± 0.045
3.01AlaGln: 3.01 ± 0.04
4.315AlaArg: 4.315 ± 0.052
5.583AlaSer: 5.583 ± 0.07
3.725AlaThr: 3.725 ± 0.065
7.907AlaVal: 7.907 ± 0.077
1.087AlaTrp: 1.087 ± 0.023
3.114AlaTyr: 3.114 ± 0.04
0.001AlaXaa: 0.001 ± 0.001
Cys
0.613CysAla: 0.613 ± 0.016
0.135CysCys: 0.135 ± 0.008
0.342CysAsp: 0.342 ± 0.014
0.382CysGlu: 0.382 ± 0.014
0.343CysPhe: 0.343 ± 0.014
0.938CysGly: 0.938 ± 0.023
0.192CysHis: 0.192 ± 0.011
0.484CysIle: 0.484 ± 0.015
0.324CysLys: 0.324 ± 0.014
0.85CysLeu: 0.85 ± 0.022
0.224CysMet: 0.224 ± 0.011
0.259CysAsn: 0.259 ± 0.012
0.408CysPro: 0.408 ± 0.017
0.241CysGln: 0.241 ± 0.01
0.621CysArg: 0.621 ± 0.019
0.554CysSer: 0.554 ± 0.016
0.416CysThr: 0.416 ± 0.015
0.488CysVal: 0.488 ± 0.017
0.102CysTrp: 0.102 ± 0.007
0.274CysTyr: 0.274 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
4.183AspAla: 4.183 ± 0.058
0.42AspCys: 0.42 ± 0.014
2.129AspAsp: 2.129 ± 0.034
3.369AspGlu: 3.369 ± 0.046
2.101AspPhe: 2.101 ± 0.032
4.662AspGly: 4.662 ± 0.063
0.944AspHis: 0.944 ± 0.02
3.131AspIle: 3.131 ± 0.04
2.588AspLys: 2.588 ± 0.037
4.33AspLeu: 4.33 ± 0.049
1.415AspMet: 1.415 ± 0.026
1.77AspAsn: 1.77 ± 0.037
2.432AspPro: 2.432 ± 0.038
1.648AspGln: 1.648 ± 0.029
2.824AspArg: 2.824 ± 0.045
2.931AspSer: 2.931 ± 0.043
2.633AspThr: 2.633 ± 0.077
3.114AspVal: 3.114 ± 0.042
0.934AspTrp: 0.934 ± 0.022
2.204AspTyr: 2.204 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
7.123GluAla: 7.123 ± 0.074
0.43GluCys: 0.43 ± 0.015
3.04GluAsp: 3.04 ± 0.035
5.979GluGlu: 5.979 ± 0.076
2.168GluPhe: 2.168 ± 0.036
4.965GluGly: 4.965 ± 0.055
1.366GluHis: 1.366 ± 0.03
3.836GluIle: 3.836 ± 0.044
3.733GluLys: 3.733 ± 0.054
7.489GluLeu: 7.489 ± 0.069
1.821GluMet: 1.821 ± 0.031
2.324GluAsn: 2.324 ± 0.034
2.495GluPro: 2.495 ± 0.033
3.206GluGln: 3.206 ± 0.046
3.981GluArg: 3.981 ± 0.058
3.248GluSer: 3.248 ± 0.038
3.437GluThr: 3.437 ± 0.044
4.003GluVal: 4.003 ± 0.042
1.049GluTrp: 1.049 ± 0.024
2.093GluTyr: 2.093 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
3.482PheAla: 3.482 ± 0.041
0.363PheCys: 0.363 ± 0.014
2.117PheAsp: 2.117 ± 0.035
2.044PheGlu: 2.044 ± 0.034
1.766PhePhe: 1.766 ± 0.034
3.16PheGly: 3.16 ± 0.042
0.937PheHis: 0.937 ± 0.024
2.463PheIle: 2.463 ± 0.043
1.761PheLys: 1.761 ± 0.032
3.923PheLeu: 3.923 ± 0.058
1.104PheMet: 1.104 ± 0.026
1.457PheAsn: 1.457 ± 0.029
1.597PhePro: 1.597 ± 0.028
1.463PheGln: 1.463 ± 0.026
2.477PheArg: 2.477 ± 0.037
2.757PheSer: 2.757 ± 0.043
2.398PheThr: 2.398 ± 0.036
2.617PheVal: 2.617 ± 0.036
0.57PheTrp: 0.57 ± 0.018
1.525PheTyr: 1.525 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
6.705GlyAla: 6.705 ± 0.088
0.828GlyCys: 0.828 ± 0.018
3.69GlyAsp: 3.69 ± 0.088
5.257GlyGlu: 5.257 ± 0.05
3.347GlyPhe: 3.347 ± 0.044
6.494GlyGly: 6.494 ± 0.083
1.492GlyHis: 1.492 ± 0.032
5.448GlyIle: 5.448 ± 0.057
4.538GlyLys: 4.538 ± 0.047
7.796GlyLeu: 7.796 ± 0.069
2.474GlyMet: 2.474 ± 0.036
2.905GlyAsn: 2.905 ± 0.053
2.275GlyPro: 2.275 ± 0.085
3.007GlyGln: 3.007 ± 0.042
4.186GlyArg: 4.186 ± 0.054
5.067GlySer: 5.067 ± 0.058
4.646GlyThr: 4.646 ± 0.068
5.287GlyVal: 5.287 ± 0.055
1.3GlyTrp: 1.3 ± 0.027
3.174GlyTyr: 3.174 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
1.485HisAla: 1.485 ± 0.027
0.208HisCys: 0.208 ± 0.011
0.875HisAsp: 0.875 ± 0.023
1.161HisGlu: 1.161 ± 0.026
0.991HisPhe: 0.991 ± 0.021
1.406HisGly: 1.406 ± 0.027
0.556HisHis: 0.556 ± 0.017
1.206HisIle: 1.206 ± 0.025
0.781HisLys: 0.781 ± 0.017
1.856HisLeu: 1.856 ± 0.039
0.556HisMet: 0.556 ± 0.019
0.686HisAsn: 0.686 ± 0.018
1.211HisPro: 1.211 ± 0.025
0.69HisGln: 0.69 ± 0.019
1.078HisArg: 1.078 ± 0.024
1.162HisSer: 1.162 ± 0.025
1.054HisThr: 1.054 ± 0.021
1.3HisVal: 1.3 ± 0.026
0.339HisTrp: 0.339 ± 0.013
0.88HisTyr: 0.88 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
5.414IleAla: 5.414 ± 0.055
0.559IleCys: 0.559 ± 0.019
2.888IleAsp: 2.888 ± 0.038
3.211IleGlu: 3.211 ± 0.047
2.12IlePhe: 2.12 ± 0.038
4.537IleGly: 4.537 ± 0.056
1.286IleHis: 1.286 ± 0.026
3.502IleIle: 3.502 ± 0.059
2.468IleLys: 2.468 ± 0.04
5.626IleLeu: 5.626 ± 0.075
1.495IleMet: 1.495 ± 0.029
2.051IleAsn: 2.051 ± 0.036
2.964IlePro: 2.964 ± 0.039
2.095IleGln: 2.095 ± 0.038
4.114IleArg: 4.114 ± 0.05
3.958IleSer: 3.958 ± 0.052
3.346IleThr: 3.346 ± 0.036
4.044IleVal: 4.044 ± 0.054
0.699IleTrp: 0.699 ± 0.016
1.925IleTyr: 1.925 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
4.492LysAla: 4.492 ± 0.052
0.249LysCys: 0.249 ± 0.01
2.704LysAsp: 2.704 ± 0.043
4.05LysGlu: 4.05 ± 0.054
1.232LysPhe: 1.232 ± 0.023
3.629LysGly: 3.629 ± 0.041
0.924LysHis: 0.924 ± 0.023
2.587LysIle: 2.587 ± 0.041
2.849LysLys: 2.849 ± 0.046
5.215LysLeu: 5.215 ± 0.058
1.291LysMet: 1.291 ± 0.027
1.924LysAsn: 1.924 ± 0.04
2.253LysPro: 2.253 ± 0.035
2.099LysGln: 2.099 ± 0.036
2.803LysArg: 2.803 ± 0.038
2.642LysSer: 2.642 ± 0.04
2.7LysThr: 2.7 ± 0.041
3.152LysVal: 3.152 ± 0.041
0.695LysTrp: 0.695 ± 0.018
1.583LysTyr: 1.583 ± 0.03
0.0LysXaa: 0.0 ± 0.0
Leu
10.016LeuAla: 10.016 ± 0.091
0.873LeuCys: 0.873 ± 0.022
5.254LeuAsp: 5.254 ± 0.054
6.841LeuGlu: 6.841 ± 0.076
4.385LeuPhe: 4.385 ± 0.061
6.751LeuGly: 6.751 ± 0.055
2.058LeuHis: 2.058 ± 0.038
5.361LeuIle: 5.361 ± 0.065
5.142LeuLys: 5.142 ± 0.054
11.341LeuLeu: 11.341 ± 0.112
2.54LeuMet: 2.54 ± 0.038
3.524LeuAsn: 3.524 ± 0.048
5.074LeuPro: 5.074 ± 0.047
3.949LeuGln: 3.949 ± 0.05
5.408LeuArg: 5.408 ± 0.059
6.783LeuSer: 6.783 ± 0.067
5.599LeuThr: 5.599 ± 0.055
6.477LeuVal: 6.477 ± 0.061
1.133LeuTrp: 1.133 ± 0.025
3.487LeuTyr: 3.487 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.457MetAla: 2.457 ± 0.033
0.161MetCys: 0.161 ± 0.009
1.576MetAsp: 1.576 ± 0.028
2.179MetGlu: 2.179 ± 0.032
0.95MetPhe: 0.95 ± 0.021
1.885MetGly: 1.885 ± 0.029
0.378MetHis: 0.378 ± 0.013
1.608MetIle: 1.608 ± 0.035
1.835MetLys: 1.835 ± 0.029
2.896MetLeu: 2.896 ± 0.04
0.837MetMet: 0.837 ± 0.02
1.323MetAsn: 1.323 ± 0.025
1.136MetPro: 1.136 ± 0.023
0.902MetGln: 0.902 ± 0.02
1.318MetArg: 1.318 ± 0.027
1.563MetSer: 1.563 ± 0.026
1.514MetThr: 1.514 ± 0.027
1.732MetVal: 1.732 ± 0.032
0.253MetTrp: 0.253 ± 0.01
0.744MetTyr: 0.744 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.838AsnAla: 2.838 ± 0.04
0.266AsnCys: 0.266 ± 0.011
1.627AsnAsp: 1.627 ± 0.034
2.093AsnGlu: 2.093 ± 0.028
1.183AsnPhe: 1.183 ± 0.023
3.567AsnGly: 3.567 ± 0.052
0.72AsnHis: 0.72 ± 0.019
2.065AsnIle: 2.065 ± 0.034
1.741AsnLys: 1.741 ± 0.038
3.238AsnLeu: 3.238 ± 0.043
0.951AsnMet: 0.951 ± 0.022
1.364AsnAsn: 1.364 ± 0.04
2.256AsnPro: 2.256 ± 0.033
1.279AsnGln: 1.279 ± 0.026
2.214AsnArg: 2.214 ± 0.033
2.032AsnSer: 2.032 ± 0.04
2.015AsnThr: 2.015 ± 0.036
2.381AsnVal: 2.381 ± 0.04
0.583AsnTrp: 0.583 ± 0.017
1.257AsnTyr: 1.257 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
4.561ProAla: 4.561 ± 0.064
0.292ProCys: 0.292 ± 0.013
2.795ProAsp: 2.795 ± 0.044
3.797ProGlu: 3.797 ± 0.045
1.838ProPhe: 1.838 ± 0.029
3.849ProGly: 3.849 ± 0.057
0.935ProHis: 0.935 ± 0.02
1.99ProIle: 1.99 ± 0.036
1.686ProLys: 1.686 ± 0.029
4.128ProLeu: 4.128 ± 0.044
1.012ProMet: 1.012 ± 0.022
1.361ProAsn: 1.361 ± 0.029
1.578ProPro: 1.578 ± 0.031
1.557ProGln: 1.557 ± 0.031
1.661ProArg: 1.661 ± 0.032
2.571ProSer: 2.571 ± 0.038
1.847ProThr: 1.847 ± 0.083
3.85ProVal: 3.85 ± 0.043
0.569ProTrp: 0.569 ± 0.018
1.639ProTyr: 1.639 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.838GlnAla: 3.838 ± 0.046
0.207GlnCys: 0.207 ± 0.009
1.682GlnAsp: 1.682 ± 0.029
2.741GlnGlu: 2.741 ± 0.041
1.393GlnPhe: 1.393 ± 0.026
2.611GlnGly: 2.611 ± 0.04
0.652GlnHis: 0.652 ± 0.018
2.148GlnIle: 2.148 ± 0.031
1.795GlnLys: 1.795 ± 0.032
4.014GlnLeu: 4.014 ± 0.048
1.06GlnMet: 1.06 ± 0.023
1.233GlnAsn: 1.233 ± 0.025
1.619GlnPro: 1.619 ± 0.031
1.532GlnGln: 1.532 ± 0.028
1.679GlnArg: 1.679 ± 0.032
2.024GlnSer: 2.024 ± 0.031
2.013GlnThr: 2.013 ± 0.034
2.703GlnVal: 2.703 ± 0.036
0.606GlnTrp: 0.606 ± 0.018
1.38GlnTyr: 1.38 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
3.628ArgAla: 3.628 ± 0.044
0.429ArgCys: 0.429 ± 0.017
2.529ArgAsp: 2.529 ± 0.037
4.217ArgGlu: 4.217 ± 0.05
2.466ArgPhe: 2.466 ± 0.036
3.111ArgGly: 3.111 ± 0.045
1.185ArgHis: 1.185 ± 0.027
3.834ArgIle: 3.834 ± 0.05
2.963ArgLys: 2.963 ± 0.044
6.472ArgLeu: 6.472 ± 0.078
1.895ArgMet: 1.895 ± 0.033
2.095ArgAsn: 2.095 ± 0.035
1.989ArgPro: 1.989 ± 0.032
2.557ArgGln: 2.557 ± 0.043
3.368ArgArg: 3.368 ± 0.054
3.22ArgSer: 3.22 ± 0.045
2.798ArgThr: 2.798 ± 0.039
3.458ArgVal: 3.458 ± 0.046
0.742ArgTrp: 0.742 ± 0.021
2.156ArgTyr: 2.156 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
5.543SerAla: 5.543 ± 0.059
0.482SerCys: 0.482 ± 0.016
2.605SerAsp: 2.605 ± 0.037
3.121SerGlu: 3.121 ± 0.039
2.979SerPhe: 2.979 ± 0.044
6.138SerGly: 6.138 ± 0.08
1.153SerHis: 1.153 ± 0.026
3.69SerIle: 3.69 ± 0.045
2.537SerLys: 2.537 ± 0.035
6.17SerLeu: 6.17 ± 0.065
1.66SerMet: 1.66 ± 0.031
1.973SerAsn: 1.973 ± 0.034
2.79SerPro: 2.79 ± 0.037
2.013SerGln: 2.013 ± 0.034
3.5SerArg: 3.5 ± 0.048
3.932SerSer: 3.932 ± 0.057
2.921SerThr: 2.921 ± 0.045
4.298SerVal: 4.298 ± 0.044
0.91SerTrp: 0.91 ± 0.021
2.302SerTyr: 2.302 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
5.508ThrAla: 5.508 ± 0.069
0.352ThrCys: 0.352 ± 0.014
2.869ThrAsp: 2.869 ± 0.042
3.067ThrGlu: 3.067 ± 0.034
2.139ThrPhe: 2.139 ± 0.035
5.347ThrGly: 5.347 ± 0.226
0.929ThrHis: 0.929 ± 0.021
3.103ThrIle: 3.103 ± 0.043
1.961ThrLys: 1.961 ± 0.036
5.353ThrLeu: 5.353 ± 0.057
1.241ThrMet: 1.241 ± 0.025
1.618ThrAsn: 1.618 ± 0.034
2.646ThrPro: 2.646 ± 0.043
1.41ThrGln: 1.41 ± 0.024
2.472ThrArg: 2.472 ± 0.033
2.752ThrSer: 2.752 ± 0.035
2.424ThrThr: 2.424 ± 0.035
4.921ThrVal: 4.921 ± 0.079
0.615ThrTrp: 0.615 ± 0.016
1.88ThrTyr: 1.88 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
5.586ValAla: 5.586 ± 0.058
0.673ValCys: 0.673 ± 0.018
3.372ValAsp: 3.372 ± 0.042
4.559ValGlu: 4.559 ± 0.056
2.959ValPhe: 2.959 ± 0.04
4.392ValGly: 4.392 ± 0.059
1.37ValHis: 1.37 ± 0.026
4.324ValIle: 4.324 ± 0.056
3.762ValLys: 3.762 ± 0.05
7.301ValLeu: 7.301 ± 0.058
1.924ValMet: 1.924 ± 0.031
2.756ValAsn: 2.756 ± 0.041
3.082ValPro: 3.082 ± 0.043
2.506ValGln: 2.506 ± 0.037
3.919ValArg: 3.919 ± 0.045
4.719ValSer: 4.719 ± 0.05
4.175ValThr: 4.175 ± 0.058
4.711ValVal: 4.711 ± 0.062
0.981ValTrp: 0.981 ± 0.023
2.672ValTyr: 2.672 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.005TrpAla: 1.005 ± 0.026
0.114TrpCys: 0.114 ± 0.007
0.669TrpAsp: 0.669 ± 0.018
0.863TrpGlu: 0.863 ± 0.02
0.591TrpPhe: 0.591 ± 0.018
0.928TrpGly: 0.928 ± 0.022
0.297TrpHis: 0.297 ± 0.012
0.772TrpIle: 0.772 ± 0.02
0.778TrpLys: 0.778 ± 0.022
1.491TrpLeu: 1.491 ± 0.03
0.473TrpMet: 0.473 ± 0.014
0.718TrpAsn: 0.718 ± 0.019
0.453TrpPro: 0.453 ± 0.017
0.534TrpGln: 0.534 ± 0.017
0.773TrpArg: 0.773 ± 0.021
0.93TrpSer: 0.93 ± 0.024
0.86TrpThr: 0.86 ± 0.021
0.909TrpVal: 0.909 ± 0.022
0.185TrpTrp: 0.185 ± 0.011
0.521TrpTyr: 0.521 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.063TyrAla: 3.063 ± 0.045
0.319TyrCys: 0.319 ± 0.013
1.894TyrAsp: 1.894 ± 0.042
2.249TyrGlu: 2.249 ± 0.031
1.572TyrPhe: 1.572 ± 0.03
2.841TyrGly: 2.841 ± 0.04
0.723TyrHis: 0.723 ± 0.021
1.973TyrIle: 1.973 ± 0.031
1.614TyrLys: 1.614 ± 0.029
3.562TyrLeu: 3.562 ± 0.046
0.916TyrMet: 0.916 ± 0.021
1.484TyrAsn: 1.484 ± 0.034
1.693TyrPro: 1.693 ± 0.029
1.233TyrGln: 1.233 ± 0.026
2.399TyrArg: 2.399 ± 0.037
2.29TyrSer: 2.29 ± 0.036
2.085TyrThr: 2.085 ± 0.033
2.388TyrVal: 2.388 ± 0.033
0.512TyrTrp: 0.512 ± 0.017
1.457TyrTyr: 1.457 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.001
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5879 proteins (2125208 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski