Amino acid dipepetide frequency for Escherichia phage 4MG

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.96AlaAla: 5.96 ± 0.474
0.687AlaCys: 0.687 ± 0.136
5.073AlaAsp: 5.073 ± 0.381
4.409AlaGlu: 4.409 ± 0.304
3.146AlaPhe: 3.146 ± 0.257
5.339AlaGly: 5.339 ± 0.455
1.285AlaHis: 1.285 ± 0.153
3.921AlaIle: 3.921 ± 0.314
5.317AlaLys: 5.317 ± 0.376
5.627AlaLeu: 5.627 ± 0.356
2.614AlaMet: 2.614 ± 0.233
3.257AlaAsn: 3.257 ± 0.315
2.481AlaPro: 2.481 ± 0.245
2.614AlaGln: 2.614 ± 0.226
3.589AlaArg: 3.589 ± 0.362
4.187AlaSer: 4.187 ± 0.349
4.497AlaThr: 4.497 ± 0.418
4.653AlaVal: 4.653 ± 0.308
1.507AlaTrp: 1.507 ± 0.176
3.146AlaTyr: 3.146 ± 0.289
0.0AlaXaa: 0.0 ± 0.0
Cys
0.709CysAla: 0.709 ± 0.121
0.199CysCys: 0.199 ± 0.064
0.975CysAsp: 0.975 ± 0.158
0.775CysGlu: 0.775 ± 0.124
0.731CysPhe: 0.731 ± 0.129
0.975CysGly: 0.975 ± 0.163
0.354CysHis: 0.354 ± 0.097
0.665CysIle: 0.665 ± 0.124
0.798CysLys: 0.798 ± 0.126
0.864CysLeu: 0.864 ± 0.128
0.222CysMet: 0.222 ± 0.068
0.62CysAsn: 0.62 ± 0.12
0.443CysPro: 0.443 ± 0.116
0.332CysGln: 0.332 ± 0.085
0.642CysArg: 0.642 ± 0.133
0.775CysSer: 0.775 ± 0.126
0.62CysThr: 0.62 ± 0.121
0.908CysVal: 0.908 ± 0.141
0.177CysTrp: 0.177 ± 0.067
0.399CysTyr: 0.399 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
4.586AspAla: 4.586 ± 0.321
0.576AspCys: 0.576 ± 0.11
4.143AspAsp: 4.143 ± 0.349
4.342AspGlu: 4.342 ± 0.343
3.257AspPhe: 3.257 ± 0.259
5.738AspGly: 5.738 ± 0.467
1.219AspHis: 1.219 ± 0.155
3.877AspIle: 3.877 ± 0.36
3.545AspLys: 3.545 ± 0.292
6.048AspLeu: 6.048 ± 0.395
1.817AspMet: 1.817 ± 0.226
3.345AspAsn: 3.345 ± 0.247
2.814AspPro: 2.814 ± 0.246
2.06AspGln: 2.06 ± 0.27
2.858AspArg: 2.858 ± 0.305
3.257AspSer: 3.257 ± 0.287
3.368AspThr: 3.368 ± 0.266
4.653AspVal: 4.653 ± 0.283
1.396AspTrp: 1.396 ± 0.203
3.102AspTyr: 3.102 ± 0.274
0.0AspXaa: 0.0 ± 0.0
Glu
5.428GluAla: 5.428 ± 0.442
0.908GluCys: 0.908 ± 0.138
4.187GluAsp: 4.187 ± 0.324
5.339GluGlu: 5.339 ± 0.396
2.636GluPhe: 2.636 ± 0.255
3.899GluGly: 3.899 ± 0.282
1.152GluHis: 1.152 ± 0.138
4.143GluIle: 4.143 ± 0.321
5.45GluLys: 5.45 ± 0.443
5.893GluLeu: 5.893 ± 0.381
2.415GluMet: 2.415 ± 0.226
3.19GluAsn: 3.19 ± 0.244
1.772GluPro: 1.772 ± 0.211
2.304GluGln: 2.304 ± 0.223
3.833GluArg: 3.833 ± 0.304
3.19GluSer: 3.19 ± 0.261
3.323GluThr: 3.323 ± 0.247
3.944GluVal: 3.944 ± 0.326
1.13GluTrp: 1.13 ± 0.2
2.415GluTyr: 2.415 ± 0.218
0.0GluXaa: 0.0 ± 0.0
Phe
2.902PheAla: 2.902 ± 0.251
0.642PheCys: 0.642 ± 0.134
3.257PheAsp: 3.257 ± 0.249
2.282PheGlu: 2.282 ± 0.222
1.795PhePhe: 1.795 ± 0.208
3.567PheGly: 3.567 ± 0.302
0.753PheHis: 0.753 ± 0.131
2.415PheIle: 2.415 ± 0.274
2.149PheLys: 2.149 ± 0.232
3.102PheLeu: 3.102 ± 0.257
1.241PheMet: 1.241 ± 0.162
2.26PheAsn: 2.26 ± 0.216
1.573PhePro: 1.573 ± 0.166
1.329PheGln: 1.329 ± 0.178
2.038PheArg: 2.038 ± 0.224
2.902PheSer: 2.902 ± 0.275
2.703PheThr: 2.703 ± 0.245
3.39PheVal: 3.39 ± 0.279
0.864PheTrp: 0.864 ± 0.18
1.484PheTyr: 1.484 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
4.675GlyAla: 4.675 ± 0.435
0.931GlyCys: 0.931 ± 0.15
4.608GlyAsp: 4.608 ± 0.573
4.653GlyGlu: 4.653 ± 0.33
3.633GlyPhe: 3.633 ± 0.293
5.251GlyGly: 5.251 ± 0.457
0.908GlyHis: 0.908 ± 0.132
4.187GlyIle: 4.187 ± 0.299
5.317GlyLys: 5.317 ± 0.374
5.073GlyLeu: 5.073 ± 0.327
2.171GlyMet: 2.171 ± 0.203
3.744GlyAsn: 3.744 ± 0.39
2.348GlyPro: 2.348 ± 0.768
2.703GlyGln: 2.703 ± 0.234
2.991GlyArg: 2.991 ± 0.239
4.187GlySer: 4.187 ± 0.365
4.276GlyThr: 4.276 ± 0.395
5.649GlyVal: 5.649 ± 0.463
1.551GlyTrp: 1.551 ± 0.202
3.478GlyTyr: 3.478 ± 0.313
0.0GlyXaa: 0.0 ± 0.0
His
1.019HisAla: 1.019 ± 0.146
0.244HisCys: 0.244 ± 0.063
0.908HisAsp: 0.908 ± 0.149
1.418HisGlu: 1.418 ± 0.203
0.931HisPhe: 0.931 ± 0.146
1.285HisGly: 1.285 ± 0.192
0.399HisHis: 0.399 ± 0.098
1.241HisIle: 1.241 ± 0.174
1.241HisLys: 1.241 ± 0.205
1.196HisLeu: 1.196 ± 0.159
0.665HisMet: 0.665 ± 0.123
0.997HisAsn: 0.997 ± 0.146
0.908HisPro: 0.908 ± 0.135
0.399HisGln: 0.399 ± 0.084
0.775HisArg: 0.775 ± 0.12
0.931HisSer: 0.931 ± 0.139
1.108HisThr: 1.108 ± 0.17
0.953HisVal: 0.953 ± 0.146
0.399HisTrp: 0.399 ± 0.092
0.864HisTyr: 0.864 ± 0.138
0.0HisXaa: 0.0 ± 0.0
Ile
4.32IleAla: 4.32 ± 0.359
0.753IleCys: 0.753 ± 0.126
4.143IleAsp: 4.143 ± 0.36
3.811IleGlu: 3.811 ± 0.355
2.459IlePhe: 2.459 ± 0.231
3.235IleGly: 3.235 ± 0.293
1.374IleHis: 1.374 ± 0.155
3.589IleIle: 3.589 ± 0.289
3.345IleLys: 3.345 ± 0.293
4.542IleLeu: 4.542 ± 0.327
1.44IleMet: 1.44 ± 0.18
2.747IleAsn: 2.747 ± 0.253
2.725IlePro: 2.725 ± 0.227
1.95IleGln: 1.95 ± 0.208
2.991IleArg: 2.991 ± 0.293
3.766IleSer: 3.766 ± 0.266
3.678IleThr: 3.678 ± 0.292
3.899IleVal: 3.899 ± 0.295
0.731IleTrp: 0.731 ± 0.112
2.215IleTyr: 2.215 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
5.583LysAla: 5.583 ± 0.469
0.709LysCys: 0.709 ± 0.135
4.963LysAsp: 4.963 ± 0.386
5.073LysGlu: 5.073 ± 0.371
2.659LysPhe: 2.659 ± 0.255
4.586LysGly: 4.586 ± 0.458
1.418LysHis: 1.418 ± 0.211
3.567LysIle: 3.567 ± 0.261
4.497LysLys: 4.497 ± 0.41
4.719LysLeu: 4.719 ± 0.327
2.326LysMet: 2.326 ± 0.205
2.947LysAsn: 2.947 ± 0.245
2.614LysPro: 2.614 ± 0.254
2.459LysGln: 2.459 ± 0.254
3.368LysArg: 3.368 ± 0.318
3.301LysSer: 3.301 ± 0.261
3.988LysThr: 3.988 ± 0.253
5.229LysVal: 5.229 ± 0.41
1.174LysTrp: 1.174 ± 0.181
2.459LysTyr: 2.459 ± 0.216
0.0LysXaa: 0.0 ± 0.0
Leu
6.07LeuAla: 6.07 ± 0.386
1.219LeuCys: 1.219 ± 0.193
5.184LeuAsp: 5.184 ± 0.324
5.937LeuGlu: 5.937 ± 0.367
2.836LeuPhe: 2.836 ± 0.298
4.409LeuGly: 4.409 ± 0.373
1.44LeuHis: 1.44 ± 0.192
4.453LeuIle: 4.453 ± 0.294
5.627LeuLys: 5.627 ± 0.379
5.76LeuLeu: 5.76 ± 0.424
2.193LeuMet: 2.193 ± 0.223
3.744LeuAsn: 3.744 ± 0.292
3.434LeuPro: 3.434 ± 0.291
2.548LeuGln: 2.548 ± 0.248
4.076LeuArg: 4.076 ± 0.329
4.763LeuSer: 4.763 ± 0.289
4.83LeuThr: 4.83 ± 0.362
5.007LeuVal: 5.007 ± 0.305
1.329LeuTrp: 1.329 ± 0.15
3.235LeuTyr: 3.235 ± 0.268
0.0LeuXaa: 0.0 ± 0.0
Met
2.459MetAla: 2.459 ± 0.255
0.443MetCys: 0.443 ± 0.092
1.374MetAsp: 1.374 ± 0.181
1.662MetGlu: 1.662 ± 0.196
1.374MetPhe: 1.374 ± 0.168
1.617MetGly: 1.617 ± 0.179
0.354MetHis: 0.354 ± 0.08
1.972MetIle: 1.972 ± 0.209
2.991MetLys: 2.991 ± 0.257
2.393MetLeu: 2.393 ± 0.204
0.931MetMet: 0.931 ± 0.16
1.219MetAsn: 1.219 ± 0.179
0.731MetPro: 0.731 ± 0.124
0.775MetGln: 0.775 ± 0.145
1.396MetArg: 1.396 ± 0.174
2.238MetSer: 2.238 ± 0.209
1.972MetThr: 1.972 ± 0.194
2.105MetVal: 2.105 ± 0.199
0.465MetTrp: 0.465 ± 0.102
1.13MetTyr: 1.13 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.345AsnAla: 3.345 ± 0.254
0.532AsnCys: 0.532 ± 0.107
2.747AsnAsp: 2.747 ± 0.226
2.393AsnGlu: 2.393 ± 0.205
2.127AsnPhe: 2.127 ± 0.207
4.497AsnGly: 4.497 ± 0.404
0.775AsnHis: 0.775 ± 0.118
3.035AsnIle: 3.035 ± 0.277
2.924AsnLys: 2.924 ± 0.251
3.656AsnLeu: 3.656 ± 0.325
1.529AsnMet: 1.529 ± 0.176
1.905AsnAsn: 1.905 ± 0.255
2.526AsnPro: 2.526 ± 0.225
1.396AsnGln: 1.396 ± 0.178
2.282AsnArg: 2.282 ± 0.21
2.415AsnSer: 2.415 ± 0.233
2.969AsnThr: 2.969 ± 0.285
3.168AsnVal: 3.168 ± 0.306
1.019AsnTrp: 1.019 ± 0.164
1.639AsnTyr: 1.639 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
2.548ProAla: 2.548 ± 0.269
0.31ProCys: 0.31 ± 0.081
2.614ProAsp: 2.614 ± 0.3
3.456ProGlu: 3.456 ± 0.295
1.795ProPhe: 1.795 ± 0.197
2.326ProGly: 2.326 ± 0.227
0.731ProHis: 0.731 ± 0.125
1.617ProIle: 1.617 ± 0.17
2.57ProLys: 2.57 ± 0.228
2.836ProLeu: 2.836 ± 0.257
0.753ProMet: 0.753 ± 0.127
1.662ProAsn: 1.662 ± 0.211
1.13ProPro: 1.13 ± 0.17
1.839ProGln: 1.839 ± 0.385
1.573ProArg: 1.573 ± 0.194
2.105ProSer: 2.105 ± 0.225
2.459ProThr: 2.459 ± 0.239
3.39ProVal: 3.39 ± 0.284
0.377ProTrp: 0.377 ± 0.09
1.617ProTyr: 1.617 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
2.393GlnAla: 2.393 ± 0.259
0.332GlnCys: 0.332 ± 0.091
1.839GlnAsp: 1.839 ± 0.171
2.171GlnGlu: 2.171 ± 0.217
1.307GlnPhe: 1.307 ± 0.185
2.902GlnGly: 2.902 ± 0.626
0.598GlnHis: 0.598 ± 0.116
1.905GlnIle: 1.905 ± 0.19
2.459GlnLys: 2.459 ± 0.249
2.614GlnLeu: 2.614 ± 0.236
0.975GlnMet: 0.975 ± 0.152
1.617GlnAsn: 1.617 ± 0.181
0.997GlnPro: 0.997 ± 0.131
1.662GlnGln: 1.662 ± 0.242
1.706GlnArg: 1.706 ± 0.187
2.282GlnSer: 2.282 ± 0.199
2.149GlnThr: 2.149 ± 0.23
2.215GlnVal: 2.215 ± 0.22
0.642GlnTrp: 0.642 ± 0.115
1.418GlnTyr: 1.418 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
3.013ArgAla: 3.013 ± 0.292
0.798ArgCys: 0.798 ± 0.158
3.633ArgAsp: 3.633 ± 0.298
3.345ArgGlu: 3.345 ± 0.331
1.817ArgPhe: 1.817 ± 0.183
3.456ArgGly: 3.456 ± 0.279
0.931ArgHis: 0.931 ± 0.133
2.636ArgIle: 2.636 ± 0.259
3.545ArgLys: 3.545 ± 0.277
4.653ArgLeu: 4.653 ± 0.319
1.307ArgMet: 1.307 ± 0.17
2.57ArgAsn: 2.57 ± 0.266
1.573ArgPro: 1.573 ± 0.189
1.861ArgGln: 1.861 ± 0.213
2.836ArgArg: 2.836 ± 0.295
2.526ArgSer: 2.526 ± 0.27
2.725ArgThr: 2.725 ± 0.224
3.345ArgVal: 3.345 ± 0.275
0.864ArgTrp: 0.864 ± 0.138
1.772ArgTyr: 1.772 ± 0.198
0.0ArgXaa: 0.0 ± 0.0
Ser
4.276SerAla: 4.276 ± 0.311
0.576SerCys: 0.576 ± 0.114
3.722SerAsp: 3.722 ± 0.267
3.39SerGlu: 3.39 ± 0.274
2.548SerPhe: 2.548 ± 0.275
5.051SerGly: 5.051 ± 0.316
0.997SerHis: 0.997 ± 0.144
3.456SerIle: 3.456 ± 0.328
3.788SerLys: 3.788 ± 0.315
4.165SerLeu: 4.165 ± 0.299
1.639SerMet: 1.639 ± 0.191
2.393SerAsn: 2.393 ± 0.223
1.972SerPro: 1.972 ± 0.193
1.927SerGln: 1.927 ± 0.242
3.057SerArg: 3.057 ± 0.245
4.143SerSer: 4.143 ± 0.461
3.102SerThr: 3.102 ± 0.335
4.387SerVal: 4.387 ± 0.324
0.886SerTrp: 0.886 ± 0.131
2.371SerTyr: 2.371 ± 0.21
0.0SerXaa: 0.0 ± 0.0
Thr
4.63ThrAla: 4.63 ± 0.454
0.554ThrCys: 0.554 ± 0.11
3.811ThrAsp: 3.811 ± 0.331
3.589ThrGlu: 3.589 ± 0.275
2.503ThrPhe: 2.503 ± 0.232
5.251ThrGly: 5.251 ± 0.582
1.108ThrHis: 1.108 ± 0.142
3.5ThrIle: 3.5 ± 0.324
4.01ThrLys: 4.01 ± 0.289
4.941ThrLeu: 4.941 ± 0.362
1.529ThrMet: 1.529 ± 0.173
2.636ThrAsn: 2.636 ± 0.281
2.769ThrPro: 2.769 ± 0.241
1.972ThrGln: 1.972 ± 0.203
2.348ThrArg: 2.348 ± 0.198
2.924ThrSer: 2.924 ± 0.292
3.966ThrThr: 3.966 ± 0.553
5.295ThrVal: 5.295 ± 0.453
1.041ThrTrp: 1.041 ± 0.171
2.083ThrTyr: 2.083 ± 0.191
0.0ThrXaa: 0.0 ± 0.0
Val
5.494ValAla: 5.494 ± 0.366
0.975ValCys: 0.975 ± 0.142
4.874ValAsp: 4.874 ± 0.343
4.63ValGlu: 4.63 ± 0.347
2.836ValPhe: 2.836 ± 0.272
4.918ValGly: 4.918 ± 0.417
1.13ValHis: 1.13 ± 0.171
4.32ValIle: 4.32 ± 0.326
4.475ValLys: 4.475 ± 0.372
4.808ValLeu: 4.808 ± 0.351
2.127ValMet: 2.127 ± 0.225
3.39ValAsn: 3.39 ± 0.264
2.503ValPro: 2.503 ± 0.244
2.127ValGln: 2.127 ± 0.227
3.656ValArg: 3.656 ± 0.295
4.542ValSer: 4.542 ± 0.357
5.273ValThr: 5.273 ± 0.44
6.292ValVal: 6.292 ± 0.378
1.063ValTrp: 1.063 ± 0.142
2.659ValTyr: 2.659 ± 0.229
0.0ValXaa: 0.0 ± 0.0
Trp
1.219TrpAla: 1.219 ± 0.177
0.31TrpCys: 0.31 ± 0.107
1.174TrpAsp: 1.174 ± 0.2
1.418TrpGlu: 1.418 ± 0.189
0.687TrpPhe: 0.687 ± 0.16
1.196TrpGly: 1.196 ± 0.192
0.244TrpHis: 0.244 ± 0.066
1.041TrpIle: 1.041 ± 0.16
1.063TrpLys: 1.063 ± 0.172
1.551TrpLeu: 1.551 ± 0.195
0.82TrpMet: 0.82 ± 0.14
0.864TrpAsn: 0.864 ± 0.148
0.576TrpPro: 0.576 ± 0.092
0.62TrpGln: 0.62 ± 0.123
0.62TrpArg: 0.62 ± 0.115
1.152TrpSer: 1.152 ± 0.174
1.019TrpThr: 1.019 ± 0.164
1.063TrpVal: 1.063 ± 0.161
0.421TrpTrp: 0.421 ± 0.103
0.82TrpTyr: 0.82 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.681TyrAla: 2.681 ± 0.218
0.487TyrCys: 0.487 ± 0.112
2.769TyrAsp: 2.769 ± 0.27
2.459TyrGlu: 2.459 ± 0.26
1.573TyrPhe: 1.573 ± 0.204
2.858TyrGly: 2.858 ± 0.258
0.709TyrHis: 0.709 ± 0.12
2.171TyrIle: 2.171 ± 0.239
2.526TyrLys: 2.526 ± 0.215
3.656TyrLeu: 3.656 ± 0.317
0.864TyrMet: 0.864 ± 0.139
1.772TyrAsn: 1.772 ± 0.244
1.817TyrPro: 1.817 ± 0.235
1.307TyrGln: 1.307 ± 0.204
2.57TyrArg: 2.57 ± 0.254
2.26TyrSer: 2.26 ± 0.258
2.437TyrThr: 2.437 ± 0.228
2.548TyrVal: 2.548 ± 0.22
0.842TyrTrp: 0.842 ± 0.123
1.484TyrTyr: 1.484 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 271 proteins (45138 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski