Antrostomus carolinensis (Chuck-will s-widow) (Caprimulgus carolinensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8957 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094MKK3|A0A094MKK3_ANTCR Neurofascin (Fragment) OS=Antrostomus carolinensis OX=279965 GN=N321_02296 PE=3 SV=1
DDD2 pKa = 5.37DDD4 pKa = 3.8VAFCVLATDDD14 pKa = 4.65EE15 pKa = 4.49EE16 pKa = 4.94DD17 pKa = 3.58EE18 pKa = 5.25DDD20 pKa = 3.36ALQIHHH26 pKa = 6.13TLIQAFCCEEE36 pKa = 3.78DDD38 pKa = 2.96DDD40 pKa = 3.95VRR42 pKa = 11.84VNDDD46 pKa = 3.46AKKK49 pKa = 10.32AAIVGPSEEE58 pKa = 4.11EE59 pKa = 4.26GEEE62 pKa = 4.11RR63 pKa = 11.84DDD65 pKa = 3.68HHH67 pKa = 6.98ILITV

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094K1S8|A0A094K1S8_ANTCR Uncharacterized protein (Fragment) OS=Antrostomus carolinensis OX=279965 GN=N321_01262 PE=4 SV=1
GG1 pKa = 7.16KK2 pKa = 10.12AFKK5 pKa = 10.51GSSGLRR11 pKa = 11.84YY12 pKa = 9.8HH13 pKa = 6.41MRR15 pKa = 11.84DD16 pKa = 3.1HH17 pKa = 6.3TGEE20 pKa = 4.05RR21 pKa = 11.84PYY23 pKa = 11.38ACPQCGKK30 pKa = 10.27AFKK33 pKa = 10.27RR34 pKa = 11.84SSLLQIHH41 pKa = 5.36QRR43 pKa = 11.84VHH45 pKa = 5.29TGLRR49 pKa = 11.84AFEE52 pKa = 4.61CSQCGLTFKK61 pKa = 9.82WASHH65 pKa = 4.99YY66 pKa = 10.12QSHH69 pKa = 6.57LRR71 pKa = 11.84QHH73 pKa = 5.68TGEE76 pKa = 4.06RR77 pKa = 11.84PYY79 pKa = 10.86RR80 pKa = 11.84CPDD83 pKa = 3.22CPKK86 pKa = 10.42AFKK89 pKa = 9.46NTSCLRR95 pKa = 11.84RR96 pKa = 11.84HH97 pKa = 5.81RR98 pKa = 11.84QLHH101 pKa = 5.07TGEE104 pKa = 4.87RR105 pKa = 11.84PHH107 pKa = 7.01ACPVCGKK114 pKa = 10.48AFAQATNLRR123 pKa = 11.84QHH125 pKa = 5.55QRR127 pKa = 11.84VHH129 pKa = 5.46TGEE132 pKa = 4.22RR133 pKa = 11.84PYY135 pKa = 11.4ACPQCGKK142 pKa = 8.54TFTHH146 pKa = 6.78SSNLQLHH153 pKa = 6.21RR154 pKa = 11.84RR155 pKa = 11.84THH157 pKa = 5.28SAARR161 pKa = 11.84PYY163 pKa = 10.48RR164 pKa = 11.84CPLCPKK170 pKa = 9.07AFVMASYY177 pKa = 10.01LQRR180 pKa = 11.84HH181 pKa = 6.07LRR183 pKa = 11.84THH185 pKa = 6.35TPAA188 pKa = 5.2

Molecular weight: 21.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8957

0

8957

3644771

33

5054

406.9

45.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.275 ± 0.021

2.275 ± 0.022

5.032 ± 0.019

7.185 ± 0.037

3.959 ± 0.019

5.775 ± 0.027

2.551 ± 0.015

5.053 ± 0.019

6.491 ± 0.029

9.695 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.012

4.202 ± 0.015

5.243 ± 0.031

4.658 ± 0.023

5.168 ± 0.022

8.269 ± 0.037

5.442 ± 0.019

6.28 ± 0.021

1.195 ± 0.009

2.996 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski