Streptomyces phage Asten

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Likavirus; unclassified Likavirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CUZ1|A0A411CUZ1_9CAUD Uncharacterized protein OS=Streptomyces phage Asten OX=2510513 GN=13 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.65AATLVVGSGNYY16 pKa = 7.74LTAPTGTAIPGDD28 pKa = 4.21LLAPASPWEE37 pKa = 4.34AVGHH41 pKa = 5.64TSLEE45 pKa = 4.33EE46 pKa = 3.9VFSISSEE53 pKa = 3.96GGEE56 pKa = 3.94ATVLGTLQNKK66 pKa = 7.6NLRR69 pKa = 11.84TKK71 pKa = 10.53YY72 pKa = 9.55SARR75 pKa = 11.84TEE77 pKa = 4.2TMTFTLQQFDD87 pKa = 3.49VDD89 pKa = 4.29GLKK92 pKa = 10.51LYY94 pKa = 10.26YY95 pKa = 10.5GSNAPVLQDD104 pKa = 3.2GTVGVPSDD112 pKa = 4.04PQPTVCAFLAIFVDD126 pKa = 4.23GDD128 pKa = 3.48NHH130 pKa = 6.56FAFYY134 pKa = 10.71APKK137 pKa = 10.65AEE139 pKa = 4.57IYY141 pKa = 10.38RR142 pKa = 11.84ADD144 pKa = 4.36DD145 pKa = 3.7LALSDD150 pKa = 4.1TEE152 pKa = 4.44SLAGLPLGVKK162 pKa = 10.1PMTHH166 pKa = 6.1GTNSWTYY173 pKa = 11.14AITPLGGGDD182 pKa = 3.16IVASGATAGTPGSFTPTGASAPANLAALQASSIVASPTSAWTTGQYY228 pKa = 10.82VDD230 pKa = 5.38LGDD233 pKa = 4.38GSDD236 pKa = 4.09AFWDD240 pKa = 3.98GDD242 pKa = 3.35SWAAGQASS250 pKa = 3.39

Molecular weight: 25.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CUY1|A0A411CUY1_9CAUD Uncharacterized protein OS=Streptomyces phage Asten OX=2510513 GN=3 PE=4 SV=1
MM1 pKa = 7.22SVQRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GVTARR12 pKa = 11.84IWKK15 pKa = 4.66TTKK18 pKa = 10.72VIDD21 pKa = 3.4NRR23 pKa = 11.84GNDD26 pKa = 3.14VHH28 pKa = 6.69VAHH31 pKa = 7.18NDD33 pKa = 3.4GPHH36 pKa = 5.66EE37 pKa = 4.11VRR39 pKa = 11.84AAFIPQRR46 pKa = 11.84SAKK49 pKa = 10.63AEE51 pKa = 4.13VPGQMQINITRR62 pKa = 11.84MIVAADD68 pKa = 3.77LEE70 pKa = 4.88GVEE73 pKa = 3.94LWSRR77 pKa = 11.84VEE79 pKa = 4.08ALGKK83 pKa = 9.82QWDD86 pKa = 4.15IVTPPAYY93 pKa = 10.07HH94 pKa = 6.56HH95 pKa = 5.93GTRR98 pKa = 11.84HH99 pKa = 4.58TRR101 pKa = 11.84HH102 pKa = 5.28WSIDD106 pKa = 2.96IRR108 pKa = 11.84EE109 pKa = 4.09RR110 pKa = 11.84PP111 pKa = 3.36

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15814

31

1161

216.6

23.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.345 ± 0.316

0.727 ± 0.121

6.494 ± 0.196

7.044 ± 0.487

3.219 ± 0.156

8.549 ± 0.392

1.948 ± 0.203

4.414 ± 0.252

4.37 ± 0.255

8.505 ± 0.448

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.152

3.105 ± 0.149

4.92 ± 0.228

3.206 ± 0.136

6.532 ± 0.413

5.805 ± 0.264

6.399 ± 0.386

7.323 ± 0.23

1.929 ± 0.143

2.959 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski