Azoarcus sp. CIB

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus; unclassified Azoarcus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1J5Y1|A0A0K1J5Y1_9RHOO EamA domain-containing protein OS=Azoarcus sp. CIB OX=198107 GN=AzCIB_2068 PE=4 SV=1
MM1 pKa = 7.66ALASLSTQQIIDD13 pKa = 3.56QLDD16 pKa = 3.53SGFHH20 pKa = 5.3WVGSTITYY28 pKa = 8.88AFPVIAADD36 pKa = 5.31LFWTDD41 pKa = 3.26EE42 pKa = 4.25GSSFRR47 pKa = 11.84PLDD50 pKa = 3.62ATQQAYY56 pKa = 10.66AEE58 pKa = 4.37VALASWDD65 pKa = 3.74EE66 pKa = 4.44LIAPSFAEE74 pKa = 4.22VSGSASDD81 pKa = 3.88VEE83 pKa = 5.51FGLTLNADD91 pKa = 3.5YY92 pKa = 11.34AHH94 pKa = 7.64AYY96 pKa = 8.27FPSYY100 pKa = 10.22GSVWFRR106 pKa = 11.84SGDD109 pKa = 3.27ASLADD114 pKa = 4.26PEE116 pKa = 4.48VGSYY120 pKa = 11.08GYY122 pKa = 8.62LTYY125 pKa = 10.84VHH127 pKa = 6.65EE128 pKa = 4.78VGHH131 pKa = 6.75ALGLDD136 pKa = 3.22HH137 pKa = 6.92MGDD140 pKa = 3.84YY141 pKa = 10.72NGYY144 pKa = 9.51APEE147 pKa = 4.2PTSYY151 pKa = 10.85QDD153 pKa = 3.39STVLSVMSYY162 pKa = 9.84FGPSYY167 pKa = 10.76GSWSAGTGQVAWADD181 pKa = 3.54WVGSDD186 pKa = 4.03GRR188 pKa = 11.84LYY190 pKa = 10.56QPQTPMLDD198 pKa = 3.24DD199 pKa = 3.52VLAIQSIYY207 pKa = 10.66GADD210 pKa = 3.44RR211 pKa = 11.84TTRR214 pKa = 11.84PGDD217 pKa = 3.25TTYY220 pKa = 11.45GFGSNVSGAMAEE232 pKa = 3.99ILDD235 pKa = 4.29FARR238 pKa = 11.84NAHH241 pKa = 6.37PVLILYY247 pKa = 8.73DD248 pKa = 3.57AGGNDD253 pKa = 3.96TLNLSGWSSGSTVDD267 pKa = 3.77IAPGAYY273 pKa = 9.71SSCNDD278 pKa = 3.31MTNNIAVAYY287 pKa = 8.36STDD290 pKa = 3.27IEE292 pKa = 4.42NVVCGDD298 pKa = 3.55GSDD301 pKa = 4.18SISGNTLANRR311 pKa = 11.84LDD313 pKa = 4.0GGGGNDD319 pKa = 3.77TIYY322 pKa = 10.7GQSGDD327 pKa = 4.5DD328 pKa = 3.56EE329 pKa = 4.87LLGGGGNDD337 pKa = 3.87HH338 pKa = 7.41LDD340 pKa = 3.66GGTGIDD346 pKa = 3.76WATLGADD353 pKa = 3.57LGSYY357 pKa = 10.27AIIYY361 pKa = 8.8DD362 pKa = 3.59AATGIFTFQNQSTGVDD378 pKa = 3.75TITGTEE384 pKa = 4.0YY385 pKa = 10.54FRR387 pKa = 11.84FANDD391 pKa = 3.07VTRR394 pKa = 11.84SANEE398 pKa = 3.99LIGGQRR404 pKa = 11.84AAPVAGITVSGTGRR418 pKa = 11.84ANVLTGTDD426 pKa = 3.64ADD428 pKa = 4.08DD429 pKa = 3.55QLFGLGGADD438 pKa = 3.56SLDD441 pKa = 3.35GGFGHH446 pKa = 7.74DD447 pKa = 4.39LLDD450 pKa = 4.19GGRR453 pKa = 11.84SADD456 pKa = 3.85RR457 pKa = 11.84LAGGNGDD464 pKa = 3.51DD465 pKa = 4.43TYY467 pKa = 11.99VVDD470 pKa = 3.9NRR472 pKa = 11.84NDD474 pKa = 3.1IVIEE478 pKa = 4.3SNGLLGGIDD487 pKa = 3.69TVRR490 pKa = 11.84STVSFALGADD500 pKa = 4.14LEE502 pKa = 4.55HH503 pKa = 6.62LTLTGRR509 pKa = 11.84ARR511 pKa = 11.84ANGAGNDD518 pKa = 3.89GNNVIVGNAGLNTLDD533 pKa = 4.35GRR535 pKa = 11.84SGDD538 pKa = 3.81DD539 pKa = 3.35TLIGAGGRR547 pKa = 11.84DD548 pKa = 3.61ILSGGAGHH556 pKa = 7.56DD557 pKa = 3.29VFRR560 pKa = 11.84FDD562 pKa = 5.9AISDD566 pKa = 3.74TSATARR572 pKa = 11.84KK573 pKa = 9.89ADD575 pKa = 4.08MISDD579 pKa = 3.92FAIGEE584 pKa = 4.24DD585 pKa = 4.37LLDD588 pKa = 5.11LSLIDD593 pKa = 5.08ANLLTAEE600 pKa = 4.46NDD602 pKa = 3.3AFDD605 pKa = 3.98ATFVAAGSAFYY616 pKa = 10.99APGQLQFAGGILYY629 pKa = 10.92GNVDD633 pKa = 3.83ADD635 pKa = 4.14AKK637 pKa = 10.96PEE639 pKa = 3.69FAINVGQLALSHH651 pKa = 6.82GDD653 pKa = 3.44LVLL656 pKa = 4.83

Molecular weight: 67.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1JD67|A0A0K1JD67_9RHOO Putative IIA component of sugar transport PTS system OS=Azoarcus sp. CIB OX=198107 GN=AzCIB_4649 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4693

0

4693

1569040

37

2929

334.3

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.581 ± 0.046

1.033 ± 0.012

5.629 ± 0.03

5.878 ± 0.033

3.621 ± 0.024

8.419 ± 0.037

2.26 ± 0.016

4.646 ± 0.027

3.095 ± 0.03

10.583 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.017

2.583 ± 0.021

5.122 ± 0.029

3.27 ± 0.019

7.567 ± 0.036

5.062 ± 0.023

4.972 ± 0.029

7.649 ± 0.033

1.383 ± 0.016

2.263 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski