Methanosalsum zhilinae (strain DSM 4017 / NBRC 107636 / OCM 62 / WeN5) (Methanohalophilus zhilinae)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosalsum; Methanosalsum zhilinae

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7XLF0|F7XLF0_METZD Kynurenine formamidase OS=Methanosalsum zhilinae (strain DSM 4017 / NBRC 107636 / OCM 62 / WeN5) OX=679901 GN=Mzhil_0489 PE=4 SV=1
MM1 pKa = 7.59IKK3 pKa = 10.85GNILRR8 pKa = 11.84ILVILIALSLFGMHH22 pKa = 6.32SVASQDD28 pKa = 3.4SEE30 pKa = 3.84PRR32 pKa = 11.84EE33 pKa = 4.12FEE35 pKa = 4.16VLIQGFDD42 pKa = 3.64YY43 pKa = 11.13DD44 pKa = 3.65PSEE47 pKa = 4.96IIAHH51 pKa = 5.71VNDD54 pKa = 3.76TVVWTNMDD62 pKa = 3.92TVRR65 pKa = 11.84HH66 pKa = 5.21TVTADD71 pKa = 3.1EE72 pKa = 4.88FDD74 pKa = 5.31SGDD77 pKa = 4.03LDD79 pKa = 3.9QGDD82 pKa = 4.05SFNYY86 pKa = 9.42TFTEE90 pKa = 4.48PGTYY94 pKa = 9.78DD95 pKa = 3.47YY96 pKa = 11.85VCIYY100 pKa = 9.75HH101 pKa = 6.87PGMRR105 pKa = 11.84GTVIVEE111 pKa = 3.93AEE113 pKa = 3.9VDD115 pKa = 3.64YY116 pKa = 11.1DD117 pKa = 4.01VPPVADD123 pKa = 4.8FTANPTSGIAPLEE136 pKa = 3.91VSFTDD141 pKa = 3.38NSDD144 pKa = 3.51NAVEE148 pKa = 3.99YY149 pKa = 10.06HH150 pKa = 6.46WDD152 pKa = 3.6FGDD155 pKa = 3.94GQTSTEE161 pKa = 4.05VNPTHH166 pKa = 6.89TYY168 pKa = 9.48EE169 pKa = 4.46LPGAYY174 pKa = 8.13TVTLTVSNEE183 pKa = 3.72VGEE186 pKa = 4.69DD187 pKa = 3.26STEE190 pKa = 3.72EE191 pKa = 4.47FITVGHH197 pKa = 7.36PDD199 pKa = 4.88DD200 pKa = 5.91DD201 pKa = 4.44PAPAPGFGLIFAIAGLLVIASLVKK225 pKa = 10.5RR226 pKa = 11.84INEE229 pKa = 4.06KK230 pKa = 10.67

Molecular weight: 25.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7XQ49|F7XQ49_METZD Tryptophan synthase alpha chain OS=Methanosalsum zhilinae (strain DSM 4017 / NBRC 107636 / OCM 62 / WeN5) OX=679901 GN=trpA PE=3 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84NTKK6 pKa = 7.84GQKK9 pKa = 8.18TRR11 pKa = 11.84LAKK14 pKa = 10.65AHH16 pKa = 5.79MQNQRR21 pKa = 11.84VPMWVIVKK29 pKa = 7.4TNRR32 pKa = 11.84SVVTHH37 pKa = 6.07PKK39 pKa = 8.16RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.92WRR44 pKa = 11.84RR45 pKa = 11.84SSLRR49 pKa = 11.84LKK51 pKa = 10.7

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1972

0

1972

610469

36

3261

309.6

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.514 ± 0.054

1.239 ± 0.028

6.319 ± 0.048

7.39 ± 0.052

3.84 ± 0.04

6.949 ± 0.058

2.017 ± 0.023

9.021 ± 0.05

6.203 ± 0.06

8.575 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.924 ± 0.028

4.588 ± 0.047

3.789 ± 0.034

2.674 ± 0.028

4.734 ± 0.046

6.926 ± 0.046

5.08 ± 0.04

6.863 ± 0.04

0.87 ± 0.021

3.484 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski