Circovirus-like genome DHCV-5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHE8|A0A190WHE8_9CIRC Uncharacterized protein OS=Circovirus-like genome DHCV-5 OX=1788454 PE=4 SV=1
MM1 pKa = 7.53SSITMTQPVTKK12 pKa = 10.47ACDD15 pKa = 3.1KK16 pKa = 9.92TNRR19 pKa = 11.84SRR21 pKa = 11.84LTCFTIFDD29 pKa = 4.08MSIDD33 pKa = 3.94LQSLASRR40 pKa = 11.84FDD42 pKa = 3.6YY43 pKa = 10.15MAVGRR48 pKa = 11.84EE49 pKa = 4.3TCPSTQRR56 pKa = 11.84EE57 pKa = 4.37HH58 pKa = 6.19FQGFAYY64 pKa = 10.37CKK66 pKa = 8.34TAQRR70 pKa = 11.84FPWWHH75 pKa = 6.17KK76 pKa = 10.42RR77 pKa = 11.84LTPHH81 pKa = 6.73HH82 pKa = 6.89FEE84 pKa = 4.09FCFGSLEE91 pKa = 4.09QNEE94 pKa = 4.99RR95 pKa = 11.84YY96 pKa = 9.59CSKK99 pKa = 10.35QGQYY103 pKa = 9.75QEE105 pKa = 5.26FGIKK109 pKa = 10.17PMGDD113 pKa = 3.15GKK115 pKa = 10.78RR116 pKa = 11.84RR117 pKa = 11.84DD118 pKa = 3.53LSEE121 pKa = 4.03VCDD124 pKa = 4.77AIVTKK129 pKa = 10.25RR130 pKa = 11.84QKK132 pKa = 10.93LSDD135 pKa = 3.26IAIQFPTVFTQYY147 pKa = 11.25HH148 pKa = 5.73NGLTKK153 pKa = 10.71LEE155 pKa = 4.53AIITKK160 pKa = 9.5PYY162 pKa = 8.53EE163 pKa = 3.74HH164 pKa = 6.66HH165 pKa = 5.95TVRR168 pKa = 11.84GTWIQGVPGAGKK180 pKa = 8.55SHH182 pKa = 6.35YY183 pKa = 10.73ARR185 pKa = 11.84THH187 pKa = 5.09FQDD190 pKa = 4.2VYY192 pKa = 11.2IKK194 pKa = 10.67AQNKK198 pKa = 7.93WFDD201 pKa = 3.81GYY203 pKa = 9.42TGQHH207 pKa = 5.85TIILEE212 pKa = 4.28DD213 pKa = 4.02FDD215 pKa = 4.66SHH217 pKa = 5.57GHH219 pKa = 5.19YY220 pKa = 10.05LAHH223 pKa = 7.26LLKK226 pKa = 10.11IWSDD230 pKa = 2.87KK231 pKa = 9.66WSCSGEE237 pKa = 4.05IKK239 pKa = 10.7GGTVALQHH247 pKa = 6.83RR248 pKa = 11.84DD249 pKa = 3.47FVVTSNFSIDD259 pKa = 4.44EE260 pKa = 4.15IFTDD264 pKa = 3.89SKK266 pKa = 10.37FAEE269 pKa = 4.91PIKK272 pKa = 10.69RR273 pKa = 11.84RR274 pKa = 11.84FKK276 pKa = 10.82LITMDD281 pKa = 3.68TEE283 pKa = 4.72YY284 pKa = 10.54ISPDD288 pKa = 3.03SS289 pKa = 3.68

Molecular weight: 33.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHE8|A0A190WHE8_9CIRC Uncharacterized protein OS=Circovirus-like genome DHCV-5 OX=1788454 PE=4 SV=1
MM1 pKa = 7.34TLLGKK6 pKa = 10.45RR7 pKa = 11.84KK8 pKa = 9.46GSGRR12 pKa = 11.84KK13 pKa = 6.64GTPRR17 pKa = 11.84KK18 pKa = 9.67RR19 pKa = 11.84PSIFARR25 pKa = 11.84RR26 pKa = 11.84PLTRR30 pKa = 11.84ARR32 pKa = 11.84NKK34 pKa = 10.21RR35 pKa = 11.84PRR37 pKa = 11.84TSKK40 pKa = 10.79LGALTTYY47 pKa = 10.2RR48 pKa = 11.84YY49 pKa = 10.34SRR51 pKa = 11.84WASTGQTHH59 pKa = 7.29DD60 pKa = 3.52VSTTSYY66 pKa = 10.51QGAATFTLDD75 pKa = 4.17DD76 pKa = 3.83VKK78 pKa = 11.14GYY80 pKa = 9.39TDD82 pKa = 4.0FTSLYY87 pKa = 8.59DD88 pKa = 3.42QFMITHH94 pKa = 6.37AQIHH98 pKa = 5.19VTLITNPDD106 pKa = 2.83SAYY109 pKa = 9.51PINGTGSSAVINPNNWFPKK128 pKa = 8.95LWYY131 pKa = 10.73VFDD134 pKa = 5.08SDD136 pKa = 5.35DD137 pKa = 4.94SSTLSLDD144 pKa = 3.94VIRR147 pKa = 11.84EE148 pKa = 4.1RR149 pKa = 11.84QGVKK153 pKa = 10.56YY154 pKa = 8.95KK155 pKa = 8.62TLRR158 pKa = 11.84PNSTMIINIKK168 pKa = 9.3PRR170 pKa = 11.84CLVQTYY176 pKa = 7.52RR177 pKa = 11.84TATSTGYY184 pKa = 10.73APRR187 pKa = 11.84RR188 pKa = 11.84MFLDD192 pKa = 3.15VATGYY197 pKa = 10.12NVPHH201 pKa = 6.74YY202 pKa = 10.22GLKK205 pKa = 10.45FCVDD209 pKa = 3.51TMGLDD214 pKa = 3.54PTDD217 pKa = 3.3KK218 pKa = 10.8FMVRR222 pKa = 11.84MEE224 pKa = 4.07TKK226 pKa = 9.77YY227 pKa = 8.69WLKK230 pKa = 10.8FKK232 pKa = 10.97GVLL235 pKa = 3.58

Molecular weight: 26.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

524

235

289

262.0

30.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.962 ± 0.09

1.908 ± 0.658

6.107 ± 0.093

3.053 ± 1.371

5.725 ± 0.915

6.489 ± 0.199

3.435 ± 1.079

5.725 ± 0.915

6.87 ± 0.038

6.298 ± 0.848

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.31

2.672 ± 0.721

4.389 ± 0.711

4.389 ± 1.143

6.87 ± 1.021

7.252 ± 0.011

10.305 ± 1.267

4.58 ± 0.592

1.908 ± 0.128

4.58 ± 0.592
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski