Amino acid dipepetide frequency for Dorea sp. AF36-15AT

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.707AlaAla: 6.707 ± 0.129
1.134AlaCys: 1.134 ± 0.04
4.799AlaAsp: 4.799 ± 0.078
6.033AlaGlu: 6.033 ± 0.098
3.08AlaPhe: 3.08 ± 0.07
6.205AlaGly: 6.205 ± 0.103
1.203AlaHis: 1.203 ± 0.041
5.28AlaIle: 5.28 ± 0.076
4.944AlaLys: 4.944 ± 0.088
6.715AlaLeu: 6.715 ± 0.101
2.611AlaMet: 2.611 ± 0.065
2.615AlaAsn: 2.615 ± 0.067
2.06AlaPro: 2.06 ± 0.054
2.166AlaGln: 2.166 ± 0.045
2.935AlaArg: 2.935 ± 0.062
4.046AlaSer: 4.046 ± 0.074
3.414AlaThr: 3.414 ± 0.089
6.351AlaVal: 6.351 ± 0.097
0.577AlaTrp: 0.577 ± 0.023
2.773AlaTyr: 2.773 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
1.093CysAla: 1.093 ± 0.035
0.267CysCys: 0.267 ± 0.02
0.861CysAsp: 0.861 ± 0.034
1.074CysGlu: 1.074 ± 0.036
0.584CysPhe: 0.584 ± 0.024
1.559CysGly: 1.559 ± 0.052
0.307CysHis: 0.307 ± 0.019
1.159CysIle: 1.159 ± 0.039
0.851CysLys: 0.851 ± 0.036
1.16CysLeu: 1.16 ± 0.039
0.51CysMet: 0.51 ± 0.028
0.607CysAsn: 0.607 ± 0.025
0.664CysPro: 0.664 ± 0.029
0.423CysGln: 0.423 ± 0.02
0.733CysArg: 0.733 ± 0.027
0.849CysSer: 0.849 ± 0.036
0.806CysThr: 0.806 ± 0.032
1.105CysVal: 1.105 ± 0.04
0.109CysTrp: 0.109 ± 0.011
0.609CysTyr: 0.609 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
4.451AspAla: 4.451 ± 0.068
0.825AspCys: 0.825 ± 0.032
3.229AspAsp: 3.229 ± 0.068
4.78AspGlu: 4.78 ± 0.077
2.342AspPhe: 2.342 ± 0.057
4.599AspGly: 4.599 ± 0.099
1.0AspHis: 1.0 ± 0.038
4.514AspIle: 4.514 ± 0.08
3.737AspLys: 3.737 ± 0.075
4.595AspLeu: 4.595 ± 0.078
2.104AspMet: 2.104 ± 0.056
2.279AspAsn: 2.279 ± 0.058
1.811AspPro: 1.811 ± 0.04
1.718AspGln: 1.718 ± 0.047
2.531AspArg: 2.531 ± 0.062
3.034AspSer: 3.034 ± 0.069
3.27AspThr: 3.27 ± 0.07
4.153AspVal: 4.153 ± 0.077
0.566AspTrp: 0.566 ± 0.024
2.791AspTyr: 2.791 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
5.726GluAla: 5.726 ± 0.108
0.951GluCys: 0.951 ± 0.031
4.564GluAsp: 4.564 ± 0.089
7.585GluGlu: 7.585 ± 0.134
2.602GluPhe: 2.602 ± 0.063
4.313GluGly: 4.313 ± 0.075
1.644GluHis: 1.644 ± 0.049
5.693GluIle: 5.693 ± 0.099
6.747GluLys: 6.747 ± 0.09
7.28GluLeu: 7.28 ± 0.11
2.685GluMet: 2.685 ± 0.059
3.773GluAsn: 3.773 ± 0.072
2.009GluPro: 2.009 ± 0.053
3.255GluGln: 3.255 ± 0.072
3.588GluArg: 3.588 ± 0.072
3.386GluSer: 3.386 ± 0.067
3.86GluThr: 3.86 ± 0.068
4.773GluVal: 4.773 ± 0.08
0.699GluTrp: 0.699 ± 0.028
3.36GluTyr: 3.36 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
2.909PheAla: 2.909 ± 0.06
0.705PheCys: 0.705 ± 0.024
2.477PheAsp: 2.477 ± 0.057
2.551PheGlu: 2.551 ± 0.058
1.498PhePhe: 1.498 ± 0.044
2.966PheGly: 2.966 ± 0.081
0.834PheHis: 0.834 ± 0.033
2.487PheIle: 2.487 ± 0.064
1.831PheLys: 1.831 ± 0.049
3.432PheLeu: 3.432 ± 0.079
1.06PheMet: 1.06 ± 0.036
1.307PheAsn: 1.307 ± 0.035
1.319PhePro: 1.319 ± 0.042
1.287PheGln: 1.287 ± 0.04
1.685PheArg: 1.685 ± 0.042
2.386PheSer: 2.386 ± 0.056
2.112PheThr: 2.112 ± 0.055
2.773PheVal: 2.773 ± 0.059
0.343PheTrp: 0.343 ± 0.021
1.547PheTyr: 1.547 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
5.309GlyAla: 5.309 ± 0.095
1.358GlyCys: 1.358 ± 0.05
3.581GlyAsp: 3.581 ± 0.062
4.883GlyGlu: 4.883 ± 0.078
2.912GlyPhe: 2.912 ± 0.064
4.951GlyGly: 4.951 ± 0.095
1.342GlyHis: 1.342 ± 0.038
6.218GlyIle: 6.218 ± 0.101
5.575GlyLys: 5.575 ± 0.085
5.754GlyLeu: 5.754 ± 0.094
2.735GlyMet: 2.735 ± 0.061
3.106GlyAsn: 3.106 ± 0.068
1.297GlyPro: 1.297 ± 0.041
2.129GlyGln: 2.129 ± 0.054
3.258GlyArg: 3.258 ± 0.064
3.955GlySer: 3.955 ± 0.074
4.42GlyThr: 4.42 ± 0.098
5.347GlyVal: 5.347 ± 0.085
0.707GlyTrp: 0.707 ± 0.04
3.344GlyTyr: 3.344 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.257HisAla: 1.257 ± 0.036
0.317HisCys: 0.317 ± 0.019
0.968HisAsp: 0.968 ± 0.042
1.214HisGlu: 1.214 ± 0.042
0.813HisPhe: 0.813 ± 0.034
1.418HisGly: 1.418 ± 0.045
0.474HisHis: 0.474 ± 0.039
1.383HisIle: 1.383 ± 0.042
1.038HisLys: 1.038 ± 0.035
1.589HisLeu: 1.589 ± 0.044
0.702HisMet: 0.702 ± 0.027
0.713HisAsn: 0.713 ± 0.032
0.906HisPro: 0.906 ± 0.04
0.591HisGln: 0.591 ± 0.028
0.906HisArg: 0.906 ± 0.034
0.945HisSer: 0.945 ± 0.04
1.117HisThr: 1.117 ± 0.035
1.284HisVal: 1.284 ± 0.042
0.147HisTrp: 0.147 ± 0.014
0.774HisTyr: 0.774 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.933IleAla: 5.933 ± 0.088
1.416IleCys: 1.416 ± 0.039
4.048IleAsp: 4.048 ± 0.063
4.919IleGlu: 4.919 ± 0.085
2.791IlePhe: 2.791 ± 0.067
5.453IleGly: 5.453 ± 0.097
1.467IleHis: 1.467 ± 0.048
4.958IleIle: 4.958 ± 0.095
3.767IleLys: 3.767 ± 0.079
6.923IleLeu: 6.923 ± 0.116
2.11IleMet: 2.11 ± 0.054
2.638IleAsn: 2.638 ± 0.055
3.168IlePro: 3.168 ± 0.059
2.402IleGln: 2.402 ± 0.06
3.938IleArg: 3.938 ± 0.084
4.504IleSer: 4.504 ± 0.082
4.189IleThr: 4.189 ± 0.079
5.11IleVal: 5.11 ± 0.087
0.588IleTrp: 0.588 ± 0.029
2.779IleTyr: 2.779 ± 0.062
0.0IleXaa: 0.0 ± 0.0
Lys
5.16LysAla: 5.16 ± 0.086
0.798LysCys: 0.798 ± 0.034
4.144LysAsp: 4.144 ± 0.088
6.901LysGlu: 6.901 ± 0.101
1.772LysPhe: 1.772 ± 0.054
4.214LysGly: 4.214 ± 0.068
1.106LysHis: 1.106 ± 0.036
4.84LysIle: 4.84 ± 0.08
6.384LysLys: 6.384 ± 0.101
5.326LysLeu: 5.326 ± 0.076
2.246LysMet: 2.246 ± 0.048
3.531LysAsn: 3.531 ± 0.071
1.967LysPro: 1.967 ± 0.046
2.363LysGln: 2.363 ± 0.051
3.23LysArg: 3.23 ± 0.075
3.246LysSer: 3.246 ± 0.068
3.601LysThr: 3.601 ± 0.071
4.502LysVal: 4.502 ± 0.088
0.599LysTrp: 0.599 ± 0.027
2.838LysTyr: 2.838 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
6.752LeuAla: 6.752 ± 0.109
1.436LeuCys: 1.436 ± 0.038
5.222LeuAsp: 5.222 ± 0.067
6.224LeuGlu: 6.224 ± 0.092
3.32LeuPhe: 3.32 ± 0.084
5.99LeuGly: 5.99 ± 0.085
1.593LeuHis: 1.593 ± 0.042
5.968LeuIle: 5.968 ± 0.095
6.037LeuLys: 6.037 ± 0.09
7.92LeuLeu: 7.92 ± 0.128
2.665LeuMet: 2.665 ± 0.06
3.691LeuAsn: 3.691 ± 0.071
3.44LeuPro: 3.44 ± 0.07
2.868LeuGln: 2.868 ± 0.07
3.618LeuArg: 3.618 ± 0.079
5.522LeuSer: 5.522 ± 0.092
5.13LeuThr: 5.13 ± 0.073
5.694LeuVal: 5.694 ± 0.086
0.679LeuTrp: 0.679 ± 0.029
3.168LeuTyr: 3.168 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.545MetAla: 2.545 ± 0.064
0.42MetCys: 0.42 ± 0.022
2.069MetAsp: 2.069 ± 0.053
2.684MetGlu: 2.684 ± 0.051
1.056MetPhe: 1.056 ± 0.037
2.165MetGly: 2.165 ± 0.056
0.532MetHis: 0.532 ± 0.024
2.326MetIle: 2.326 ± 0.053
2.786MetLys: 2.786 ± 0.064
2.935MetLeu: 2.935 ± 0.061
1.051MetMet: 1.051 ± 0.04
1.7MetAsn: 1.7 ± 0.044
1.138MetPro: 1.138 ± 0.036
1.245MetGln: 1.245 ± 0.044
1.429MetArg: 1.429 ± 0.046
1.897MetSer: 1.897 ± 0.04
1.833MetThr: 1.833 ± 0.048
2.044MetVal: 2.044 ± 0.048
0.195MetTrp: 0.195 ± 0.016
1.006MetTyr: 1.006 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.124AsnAla: 3.124 ± 0.065
0.621AsnCys: 0.621 ± 0.026
2.053AsnAsp: 2.053 ± 0.055
2.708AsnGlu: 2.708 ± 0.057
1.347AsnPhe: 1.347 ± 0.044
3.495AsnGly: 3.495 ± 0.07
0.876AsnHis: 0.876 ± 0.034
3.129AsnIle: 3.129 ± 0.064
2.466AsnLys: 2.466 ± 0.069
3.601AsnLeu: 3.601 ± 0.069
1.395AsnMet: 1.395 ± 0.043
1.642AsnAsn: 1.642 ± 0.046
1.944AsnPro: 1.944 ± 0.044
1.468AsnGln: 1.468 ± 0.04
2.084AsnArg: 2.084 ± 0.05
2.154AsnSer: 2.154 ± 0.06
2.247AsnThr: 2.247 ± 0.057
3.017AsnVal: 3.017 ± 0.068
0.384AsnTrp: 0.384 ± 0.02
1.787AsnTyr: 1.787 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
2.371ProAla: 2.371 ± 0.058
0.455ProCys: 0.455 ± 0.025
2.344ProAsp: 2.344 ± 0.057
3.564ProGlu: 3.564 ± 0.068
1.442ProPhe: 1.442 ± 0.042
2.393ProGly: 2.393 ± 0.056
0.601ProHis: 0.601 ± 0.026
2.2ProIle: 2.2 ± 0.054
2.012ProLys: 2.012 ± 0.057
2.531ProLeu: 2.531 ± 0.053
0.933ProMet: 0.933 ± 0.034
1.24ProAsn: 1.24 ± 0.038
0.673ProPro: 0.673 ± 0.029
0.913ProGln: 0.913 ± 0.033
0.972ProArg: 0.972 ± 0.031
1.76ProSer: 1.76 ± 0.046
1.625ProThr: 1.625 ± 0.046
2.883ProVal: 2.883 ± 0.056
0.283ProTrp: 0.283 ± 0.017
1.429ProTyr: 1.429 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.547GlnAla: 2.547 ± 0.053
0.404GlnCys: 0.404 ± 0.021
1.601GlnAsp: 1.601 ± 0.043
2.722GlnGlu: 2.722 ± 0.062
1.147GlnPhe: 1.147 ± 0.036
2.05GlnGly: 2.05 ± 0.051
0.51GlnHis: 0.51 ± 0.024
2.816GlnIle: 2.816 ± 0.068
2.736GlnLys: 2.736 ± 0.057
2.747GlnLeu: 2.747 ± 0.05
1.308GlnMet: 1.308 ± 0.043
1.6GlnAsn: 1.6 ± 0.043
0.895GlnPro: 0.895 ± 0.03
1.212GlnGln: 1.212 ± 0.038
1.416GlnArg: 1.416 ± 0.043
1.63GlnSer: 1.63 ± 0.048
1.783GlnThr: 1.783 ± 0.052
2.243GlnVal: 2.243 ± 0.051
0.282GlnTrp: 0.282 ± 0.02
1.302GlnTyr: 1.302 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.825ArgAla: 2.825 ± 0.062
0.608ArgCys: 0.608 ± 0.027
2.347ArgAsp: 2.347 ± 0.055
3.949ArgGlu: 3.949 ± 0.083
1.667ArgPhe: 1.667 ± 0.05
2.545ArgGly: 2.545 ± 0.054
0.801ArgHis: 0.801 ± 0.028
3.605ArgIle: 3.605 ± 0.076
3.694ArgLys: 3.694 ± 0.064
3.799ArgLeu: 3.799 ± 0.08
1.779ArgMet: 1.779 ± 0.053
1.965ArgAsn: 1.965 ± 0.048
1.302ArgPro: 1.302 ± 0.04
1.707ArgGln: 1.707 ± 0.051
2.412ArgArg: 2.412 ± 0.063
2.204ArgSer: 2.204 ± 0.056
2.314ArgThr: 2.314 ± 0.055
2.777ArgVal: 2.777 ± 0.058
0.385ArgTrp: 0.385 ± 0.021
2.011ArgTyr: 2.011 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
4.122SerAla: 4.122 ± 0.07
0.798SerCys: 0.798 ± 0.036
3.36SerAsp: 3.36 ± 0.079
3.914SerGlu: 3.914 ± 0.082
2.22SerPhe: 2.22 ± 0.057
4.873SerGly: 4.873 ± 0.09
1.03SerHis: 1.03 ± 0.037
3.738SerIle: 3.738 ± 0.068
3.331SerLys: 3.331 ± 0.064
4.567SerLeu: 4.567 ± 0.083
1.828SerMet: 1.828 ± 0.044
2.124SerAsn: 2.124 ± 0.064
1.606SerPro: 1.606 ± 0.043
1.739SerGln: 1.739 ± 0.05
2.566SerArg: 2.566 ± 0.051
3.129SerSer: 3.129 ± 0.084
2.689SerThr: 2.689 ± 0.062
4.382SerVal: 4.382 ± 0.08
0.499SerTrp: 0.499 ± 0.024
2.217SerTyr: 2.217 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.432ThrAla: 4.432 ± 0.086
0.712ThrCys: 0.712 ± 0.03
3.44ThrAsp: 3.44 ± 0.069
4.075ThrGlu: 4.075 ± 0.077
2.168ThrPhe: 2.168 ± 0.051
4.861ThrGly: 4.861 ± 0.091
0.92ThrHis: 0.92 ± 0.029
3.929ThrIle: 3.929 ± 0.078
3.117ThrLys: 3.117 ± 0.064
4.852ThrLeu: 4.852 ± 0.078
1.555ThrMet: 1.555 ± 0.041
1.995ThrAsn: 1.995 ± 0.062
2.149ThrPro: 2.149 ± 0.059
1.391ThrGln: 1.391 ± 0.043
2.089ThrArg: 2.089 ± 0.057
2.956ThrSer: 2.956 ± 0.068
2.859ThrThr: 2.859 ± 0.071
4.526ThrVal: 4.526 ± 0.121
0.475ThrTrp: 0.475 ± 0.028
2.189ThrTyr: 2.189 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
5.091ValAla: 5.091 ± 0.085
1.307ValCys: 1.307 ± 0.041
4.027ValAsp: 4.027 ± 0.078
4.933ValGlu: 4.933 ± 0.091
2.77ValPhe: 2.77 ± 0.06
4.52ValGly: 4.52 ± 0.081
1.24ValHis: 1.24 ± 0.039
5.579ValIle: 5.579 ± 0.097
4.756ValLys: 4.756 ± 0.084
6.634ValLeu: 6.634 ± 0.111
2.256ValMet: 2.256 ± 0.046
2.82ValAsn: 2.82 ± 0.057
2.571ValPro: 2.571 ± 0.054
2.113ValGln: 2.113 ± 0.043
2.974ValArg: 2.974 ± 0.062
4.487ValSer: 4.487 ± 0.074
4.67ValThr: 4.67 ± 0.148
5.136ValVal: 5.136 ± 0.094
0.655ValTrp: 0.655 ± 0.029
2.677ValTyr: 2.677 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.467TrpAla: 0.467 ± 0.024
0.156TrpCys: 0.156 ± 0.014
0.491TrpAsp: 0.491 ± 0.026
0.633TrpGlu: 0.633 ± 0.026
0.341TrpPhe: 0.341 ± 0.02
0.615TrpGly: 0.615 ± 0.023
0.151TrpHis: 0.151 ± 0.015
0.646TrpIle: 0.646 ± 0.028
0.755TrpLys: 0.755 ± 0.028
0.84TrpLeu: 0.84 ± 0.034
0.319TrpMet: 0.319 ± 0.017
0.475TrpAsn: 0.475 ± 0.025
0.172TrpPro: 0.172 ± 0.015
0.356TrpGln: 0.356 ± 0.021
0.356TrpArg: 0.356 ± 0.02
0.431TrpSer: 0.431 ± 0.022
0.444TrpThr: 0.444 ± 0.023
0.463TrpVal: 0.463 ± 0.024
0.094TrpTrp: 0.094 ± 0.012
0.401TrpTyr: 0.401 ± 0.035
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.829TyrAla: 2.829 ± 0.05
0.642TyrCys: 0.642 ± 0.025
2.701TyrAsp: 2.701 ± 0.06
3.148TyrGlu: 3.148 ± 0.064
1.637TyrPhe: 1.637 ± 0.044
2.996TyrGly: 2.996 ± 0.053
0.923TyrHis: 0.923 ± 0.039
2.631TyrIle: 2.631 ± 0.055
2.205TyrLys: 2.205 ± 0.052
3.74TyrLeu: 3.74 ± 0.07
1.207TyrMet: 1.207 ± 0.037
1.722TyrAsn: 1.722 ± 0.049
1.531TyrPro: 1.531 ± 0.042
1.604TyrGln: 1.604 ± 0.044
1.976TyrArg: 1.976 ± 0.051
2.21TyrSer: 2.21 ± 0.05
2.308TyrThr: 2.308 ± 0.067
2.653TyrVal: 2.653 ± 0.056
0.34TyrTrp: 0.34 ± 0.021
2.023TyrTyr: 2.023 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2785 proteins (871764 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski