Amino acid dipepetide frequency for Thermus phage phiLo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.599AlaAla: 1.599 ± 0.219
0.339AlaCys: 0.339 ± 0.077
1.957AlaAsp: 1.957 ± 0.203
2.86AlaGlu: 2.86 ± 0.207
2.954AlaPhe: 2.954 ± 0.289
2.408AlaGly: 2.408 ± 0.261
1.148AlaHis: 1.148 ± 0.238
3.951AlaIle: 3.951 ± 0.287
4.44AlaLys: 4.44 ± 0.347
6.66AlaLeu: 6.66 ± 0.455
1.336AlaMet: 1.336 ± 0.168
1.976AlaAsn: 1.976 ± 0.181
2.22AlaPro: 2.22 ± 0.22
1.468AlaGln: 1.468 ± 0.195
3.782AlaArg: 3.782 ± 0.296
5.099AlaSer: 5.099 ± 0.311
3.443AlaThr: 3.443 ± 0.262
2.879AlaVal: 2.879 ± 0.263
0.621AlaTrp: 0.621 ± 0.092
2.54AlaTyr: 2.54 ± 0.212
0.0AlaXaa: 0.0 ± 0.0
Cys
0.094CysAla: 0.094 ± 0.042
0.038CysCys: 0.038 ± 0.025
0.245CysAsp: 0.245 ± 0.063
0.301CysGlu: 0.301 ± 0.07
0.207CysPhe: 0.207 ± 0.068
0.64CysGly: 0.64 ± 0.134
0.132CysHis: 0.132 ± 0.051
0.169CysIle: 0.169 ± 0.048
0.263CysLys: 0.263 ± 0.076
0.452CysLeu: 0.452 ± 0.093
0.113CysMet: 0.113 ± 0.052
0.188CysAsn: 0.188 ± 0.047
0.715CysPro: 0.715 ± 0.123
0.169CysGln: 0.169 ± 0.055
0.376CysArg: 0.376 ± 0.085
0.282CysSer: 0.282 ± 0.076
0.132CysThr: 0.132 ± 0.044
0.282CysVal: 0.282 ± 0.067
0.056CysTrp: 0.056 ± 0.031
0.188CysTyr: 0.188 ± 0.059
0.0CysXaa: 0.0 ± 0.0
Asp
2.371AspAla: 2.371 ± 0.187
0.151AspCys: 0.151 ± 0.05
2.427AspAsp: 2.427 ± 0.254
3.669AspGlu: 3.669 ± 0.34
2.822AspPhe: 2.822 ± 0.24
3.688AspGly: 3.688 ± 0.306
2.07AspHis: 2.07 ± 0.683
3.255AspIle: 3.255 ± 0.282
2.879AspLys: 2.879 ± 0.244
6.359AspLeu: 6.359 ± 0.375
0.79AspMet: 0.79 ± 0.102
1.242AspAsn: 1.242 ± 0.137
3.255AspPro: 3.255 ± 0.274
1.75AspGln: 1.75 ± 0.227
2.841AspArg: 2.841 ± 0.212
3.857AspSer: 3.857 ± 0.308
1.994AspThr: 1.994 ± 0.289
2.992AspVal: 2.992 ± 0.245
0.734AspTrp: 0.734 ± 0.119
3.01AspTyr: 3.01 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
5.833GluAla: 5.833 ± 0.352
0.226GluCys: 0.226 ± 0.066
4.854GluAsp: 4.854 ± 0.436
11.458GluGlu: 11.458 ± 0.601
3.104GluPhe: 3.104 ± 0.275
7.451GluGly: 7.451 ± 0.528
1.223GluHis: 1.223 ± 0.193
4.008GluIle: 4.008 ± 0.302
5.682GluLys: 5.682 ± 0.371
6.679GluLeu: 6.679 ± 0.356
1.43GluMet: 1.43 ± 0.191
2.86GluAsn: 2.86 ± 0.191
2.916GluPro: 2.916 ± 0.217
1.524GluGln: 1.524 ± 0.188
5.419GluArg: 5.419 ± 0.4
4.61GluSer: 4.61 ± 0.379
2.992GluThr: 2.992 ± 0.21
7.996GluVal: 7.996 ± 0.456
1.148GluTrp: 1.148 ± 0.142
2.803GluTyr: 2.803 ± 0.253
0.0GluXaa: 0.0 ± 0.0
Phe
1.881PheAla: 1.881 ± 0.199
0.508PheCys: 0.508 ± 0.098
2.596PheAsp: 2.596 ± 0.241
2.766PheGlu: 2.766 ± 0.278
2.954PhePhe: 2.954 ± 0.269
3.368PheGly: 3.368 ± 0.268
1.091PheHis: 1.091 ± 0.142
2.032PheIle: 2.032 ± 0.177
2.088PheLys: 2.088 ± 0.177
5.174PheLeu: 5.174 ± 0.375
0.508PheMet: 0.508 ± 0.109
1.223PheAsn: 1.223 ± 0.143
3.01PhePro: 3.01 ± 0.281
1.129PheGln: 1.129 ± 0.148
2.54PheArg: 2.54 ± 0.191
5.23PheSer: 5.23 ± 0.371
1.675PheThr: 1.675 ± 0.175
3.801PheVal: 3.801 ± 0.297
0.602PheTrp: 0.602 ± 0.115
2.841PheTyr: 2.841 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
3.217GlyAla: 3.217 ± 0.257
0.245GlyCys: 0.245 ± 0.074
4.12GlyAsp: 4.12 ± 0.416
7.168GlyGlu: 7.168 ± 0.528
3.161GlyPhe: 3.161 ± 0.227
6.058GlyGly: 6.058 ± 0.528
1.035GlyHis: 1.035 ± 0.142
3.876GlyIle: 3.876 ± 0.255
5.663GlyLys: 5.663 ± 0.344
5.851GlyLeu: 5.851 ± 0.333
1.223GlyMet: 1.223 ± 0.144
2.634GlyAsn: 2.634 ± 0.292
2.634GlyPro: 2.634 ± 0.24
2.088GlyGln: 2.088 ± 0.218
4.102GlyArg: 4.102 ± 0.27
5.833GlySer: 5.833 ± 0.374
3.65GlyThr: 3.65 ± 0.312
6.002GlyVal: 6.002 ± 0.342
1.072GlyTrp: 1.072 ± 0.164
2.935GlyTyr: 2.935 ± 0.222
0.0GlyXaa: 0.0 ± 0.0
His
1.072HisAla: 1.072 ± 0.193
0.094HisCys: 0.094 ± 0.044
0.696HisAsp: 0.696 ± 0.131
1.223HisGlu: 1.223 ± 0.125
1.129HisPhe: 1.129 ± 0.162
1.675HisGly: 1.675 ± 0.391
0.376HisHis: 0.376 ± 0.092
1.261HisIle: 1.261 ± 0.161
1.035HisLys: 1.035 ± 0.144
2.54HisLeu: 2.54 ± 0.325
0.357HisMet: 0.357 ± 0.076
0.602HisAsn: 0.602 ± 0.134
0.978HisPro: 0.978 ± 0.153
0.941HisGln: 0.941 ± 0.268
1.091HisArg: 1.091 ± 0.151
1.468HisSer: 1.468 ± 0.416
0.978HisThr: 0.978 ± 0.218
1.223HisVal: 1.223 ± 0.141
0.207HisTrp: 0.207 ± 0.057
1.072HisTyr: 1.072 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
3.029IleAla: 3.029 ± 0.225
0.188IleCys: 0.188 ± 0.061
2.69IleAsp: 2.69 ± 0.245
4.177IleGlu: 4.177 ± 0.283
2.145IlePhe: 2.145 ± 0.238
3.518IleGly: 3.518 ± 0.259
1.016IleHis: 1.016 ± 0.162
1.938IleIle: 1.938 ± 0.209
3.048IleLys: 3.048 ± 0.233
5.212IleLeu: 5.212 ± 0.345
0.621IleMet: 0.621 ± 0.108
2.126IleAsn: 2.126 ± 0.216
3.405IlePro: 3.405 ± 0.249
1.11IleGln: 1.11 ± 0.117
3.161IleArg: 3.161 ± 0.226
5.362IleSer: 5.362 ± 0.416
2.164IleThr: 2.164 ± 0.224
3.217IleVal: 3.217 ± 0.214
0.414IleTrp: 0.414 ± 0.088
2.484IleTyr: 2.484 ± 0.234
0.0IleXaa: 0.0 ± 0.0
Lys
4.252LysAla: 4.252 ± 0.33
0.151LysCys: 0.151 ± 0.056
3.725LysAsp: 3.725 ± 0.261
6.773LysGlu: 6.773 ± 0.424
2.333LysPhe: 2.333 ± 0.271
4.421LysGly: 4.421 ± 0.364
1.016LysHis: 1.016 ± 0.144
3.067LysIle: 3.067 ± 0.264
4.704LysLys: 4.704 ± 0.413
4.741LysLeu: 4.741 ± 0.35
1.148LysMet: 1.148 ± 0.143
2.201LysAsn: 2.201 ± 0.188
2.596LysPro: 2.596 ± 0.228
1.411LysGln: 1.411 ± 0.164
4.478LysArg: 4.478 ± 0.355
4.196LysSer: 4.196 ± 0.335
3.086LysThr: 3.086 ± 0.227
5.757LysVal: 5.757 ± 0.39
0.564LysTrp: 0.564 ± 0.109
2.615LysTyr: 2.615 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
6.642LeuAla: 6.642 ± 0.426
0.508LeuCys: 0.508 ± 0.105
5.87LeuAsp: 5.87 ± 0.381
8.128LeuGlu: 8.128 ± 0.477
4.892LeuPhe: 4.892 ± 0.311
7.526LeuGly: 7.526 ± 0.433
1.787LeuHis: 1.787 ± 0.206
4.12LeuIle: 4.12 ± 0.271
5.908LeuLys: 5.908 ± 0.404
10.63LeuLeu: 10.63 ± 0.479
1.261LeuMet: 1.261 ± 0.178
3.537LeuAsn: 3.537 ± 0.302
5.4LeuPro: 5.4 ± 0.3
2.502LeuGln: 2.502 ± 0.232
6.416LeuArg: 6.416 ± 0.36
11.176LeuSer: 11.176 ± 0.576
3.65LeuThr: 3.65 ± 0.256
8.034LeuVal: 8.034 ± 0.472
1.355LeuTrp: 1.355 ± 0.19
3.65LeuTyr: 3.65 ± 0.304
0.0LeuXaa: 0.0 ± 0.0
Met
1.505MetAla: 1.505 ± 0.147
0.019MetCys: 0.019 ± 0.019
1.129MetAsp: 1.129 ± 0.17
1.731MetGlu: 1.731 ± 0.189
0.508MetPhe: 0.508 ± 0.091
1.129MetGly: 1.129 ± 0.166
0.207MetHis: 0.207 ± 0.06
0.978MetIle: 0.978 ± 0.159
0.847MetLys: 0.847 ± 0.121
1.317MetLeu: 1.317 ± 0.166
0.207MetMet: 0.207 ± 0.064
0.583MetAsn: 0.583 ± 0.096
0.753MetPro: 0.753 ± 0.113
0.245MetGln: 0.245 ± 0.068
0.884MetArg: 0.884 ± 0.127
1.204MetSer: 1.204 ± 0.135
0.677MetThr: 0.677 ± 0.106
1.656MetVal: 1.656 ± 0.174
0.132MetTrp: 0.132 ± 0.051
0.489MetTyr: 0.489 ± 0.094
0.0MetXaa: 0.0 ± 0.0
Asn
1.844AsnAla: 1.844 ± 0.176
0.094AsnCys: 0.094 ± 0.031
1.486AsnAsp: 1.486 ± 0.196
1.712AsnGlu: 1.712 ± 0.191
1.392AsnPhe: 1.392 ± 0.139
2.521AsnGly: 2.521 ± 0.251
0.696AsnHis: 0.696 ± 0.121
1.787AsnIle: 1.787 ± 0.225
2.107AsnLys: 2.107 ± 0.191
4.666AsnLeu: 4.666 ± 0.317
0.376AsnMet: 0.376 ± 0.099
0.922AsnAsn: 0.922 ± 0.143
2.69AsnPro: 2.69 ± 0.207
1.167AsnGln: 1.167 ± 0.134
1.881AsnArg: 1.881 ± 0.176
2.164AsnSer: 2.164 ± 0.246
1.298AsnThr: 1.298 ± 0.155
2.07AsnVal: 2.07 ± 0.204
0.376AsnTrp: 0.376 ± 0.089
1.825AsnTyr: 1.825 ± 0.179
0.0AsnXaa: 0.0 ± 0.0
Pro
1.938ProAla: 1.938 ± 0.211
0.339ProCys: 0.339 ± 0.085
2.088ProAsp: 2.088 ± 0.225
4.309ProGlu: 4.309 ± 0.274
2.22ProPhe: 2.22 ± 0.236
4.045ProGly: 4.045 ± 0.277
1.185ProHis: 1.185 ± 0.258
2.822ProIle: 2.822 ± 0.231
3.838ProLys: 3.838 ± 0.286
5.155ProLeu: 5.155 ± 0.317
0.583ProMet: 0.583 ± 0.112
1.919ProAsn: 1.919 ± 0.202
3.236ProPro: 3.236 ± 0.337
1.392ProGln: 1.392 ± 0.215
2.973ProArg: 2.973 ± 0.27
3.895ProSer: 3.895 ± 0.301
2.709ProThr: 2.709 ± 0.204
3.669ProVal: 3.669 ± 0.26
0.696ProTrp: 0.696 ± 0.112
2.389ProTyr: 2.389 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
1.712GlnAla: 1.712 ± 0.213
0.113GlnCys: 0.113 ± 0.046
2.107GlnAsp: 2.107 ± 0.3
2.333GlnGlu: 2.333 ± 0.211
0.696GlnPhe: 0.696 ± 0.107
1.976GlnGly: 1.976 ± 0.239
0.339GlnHis: 0.339 ± 0.085
1.336GlnIle: 1.336 ± 0.186
1.599GlnLys: 1.599 ± 0.187
1.562GlnLeu: 1.562 ± 0.181
0.564GlnMet: 0.564 ± 0.116
0.903GlnAsn: 0.903 ± 0.137
0.941GlnPro: 0.941 ± 0.12
0.47GlnGln: 0.47 ± 0.132
1.58GlnArg: 1.58 ± 0.187
1.524GlnSer: 1.524 ± 0.179
0.978GlnThr: 0.978 ± 0.115
2.634GlnVal: 2.634 ± 0.255
0.207GlnTrp: 0.207 ± 0.075
1.11GlnTyr: 1.11 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
3.387ArgAla: 3.387 ± 0.235
0.357ArgCys: 0.357 ± 0.091
2.615ArgAsp: 2.615 ± 0.257
6.491ArgGlu: 6.491 ± 0.414
2.992ArgPhe: 2.992 ± 0.234
4.534ArgGly: 4.534 ± 0.333
0.771ArgHis: 0.771 ± 0.109
3.349ArgIle: 3.349 ± 0.237
3.857ArgLys: 3.857 ± 0.294
5.851ArgLeu: 5.851 ± 0.375
1.129ArgMet: 1.129 ± 0.147
2.333ArgAsn: 2.333 ± 0.205
2.295ArgPro: 2.295 ± 0.245
1.298ArgGln: 1.298 ± 0.184
3.876ArgArg: 3.876 ± 0.303
4.045ArgSer: 4.045 ± 0.353
2.201ArgThr: 2.201 ± 0.208
5.644ArgVal: 5.644 ± 0.302
0.828ArgTrp: 0.828 ± 0.135
3.217ArgTyr: 3.217 ± 0.278
0.0ArgXaa: 0.0 ± 0.0
Ser
3.104SerAla: 3.104 ± 0.226
0.508SerCys: 0.508 ± 0.093
4.008SerAsp: 4.008 ± 0.487
5.682SerGlu: 5.682 ± 0.366
4.61SerPhe: 4.61 ± 0.38
5.964SerGly: 5.964 ± 0.407
1.656SerHis: 1.656 ± 0.322
4.102SerIle: 4.102 ± 0.301
5.099SerLys: 5.099 ± 0.369
10.687SerLeu: 10.687 ± 0.652
1.543SerMet: 1.543 ± 0.174
2.183SerAsn: 2.183 ± 0.209
4.986SerPro: 4.986 ± 0.299
2.164SerGln: 2.164 ± 0.192
4.591SerArg: 4.591 ± 0.325
9.182SerSer: 9.182 ± 0.698
3.18SerThr: 3.18 ± 0.278
5.908SerVal: 5.908 ± 0.401
1.261SerTrp: 1.261 ± 0.157
3.706SerTyr: 3.706 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
1.881ThrAla: 1.881 ± 0.182
0.263ThrCys: 0.263 ± 0.074
2.013ThrAsp: 2.013 ± 0.276
2.897ThrGlu: 2.897 ± 0.235
2.333ThrPhe: 2.333 ± 0.229
3.293ThrGly: 3.293 ± 0.293
0.997ThrHis: 0.997 ± 0.191
2.446ThrIle: 2.446 ± 0.227
2.183ThrLys: 2.183 ± 0.212
4.779ThrLeu: 4.779 ± 0.334
0.809ThrMet: 0.809 ± 0.133
1.468ThrAsn: 1.468 ± 0.181
2.559ThrPro: 2.559 ± 0.348
0.96ThrGln: 0.96 ± 0.124
2.183ThrArg: 2.183 ± 0.21
3.481ThrSer: 3.481 ± 0.216
2.277ThrThr: 2.277 ± 0.241
2.578ThrVal: 2.578 ± 0.245
0.621ThrTrp: 0.621 ± 0.117
1.863ThrTyr: 1.863 ± 0.223
0.0ThrXaa: 0.0 ± 0.0
Val
5.287ValAla: 5.287 ± 0.325
0.564ValCys: 0.564 ± 0.098
4.384ValAsp: 4.384 ± 0.297
6.51ValGlu: 6.51 ± 0.337
3.801ValPhe: 3.801 ± 0.333
4.572ValGly: 4.572 ± 0.355
1.844ValHis: 1.844 ± 0.228
3.575ValIle: 3.575 ± 0.269
4.817ValLys: 4.817 ± 0.364
7.714ValLeu: 7.714 ± 0.433
1.242ValMet: 1.242 ± 0.112
2.07ValAsn: 2.07 ± 0.232
4.102ValPro: 4.102 ± 0.336
1.618ValGln: 1.618 ± 0.18
5.024ValArg: 5.024 ± 0.313
6.867ValSer: 6.867 ± 0.401
1.75ValThr: 1.75 ± 0.197
6.905ValVal: 6.905 ± 0.382
0.997ValTrp: 0.997 ± 0.147
3.838ValTyr: 3.838 ± 0.301
0.0ValXaa: 0.0 ± 0.0
Trp
0.659TrpAla: 0.659 ± 0.107
0.038TrpCys: 0.038 ± 0.024
0.734TrpAsp: 0.734 ± 0.133
1.167TrpGlu: 1.167 ± 0.178
0.659TrpPhe: 0.659 ± 0.111
0.865TrpGly: 0.865 ± 0.11
0.132TrpHis: 0.132 ± 0.056
0.847TrpIle: 0.847 ± 0.138
1.016TrpLys: 1.016 ± 0.157
0.997TrpLeu: 0.997 ± 0.134
0.263TrpMet: 0.263 ± 0.056
0.452TrpAsn: 0.452 ± 0.083
0.301TrpPro: 0.301 ± 0.104
0.245TrpGln: 0.245 ± 0.063
1.11TrpArg: 1.11 ± 0.154
0.884TrpSer: 0.884 ± 0.14
0.602TrpThr: 0.602 ± 0.115
1.11TrpVal: 1.11 ± 0.144
0.245TrpTrp: 0.245 ± 0.066
0.602TrpTyr: 0.602 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.371TyrAla: 2.371 ± 0.251
0.301TyrCys: 0.301 ± 0.082
2.408TyrAsp: 2.408 ± 0.252
2.86TyrGlu: 2.86 ± 0.229
2.107TyrPhe: 2.107 ± 0.244
2.916TyrGly: 2.916 ± 0.234
1.298TyrHis: 1.298 ± 0.205
2.107TyrIle: 2.107 ± 0.172
2.164TyrLys: 2.164 ± 0.211
5.983TyrLeu: 5.983 ± 0.37
0.677TyrMet: 0.677 ± 0.099
1.656TyrAsn: 1.656 ± 0.171
2.408TyrPro: 2.408 ± 0.21
1.035TyrGln: 1.035 ± 0.127
2.766TyrArg: 2.766 ± 0.248
3.763TyrSer: 3.763 ± 0.354
2.446TyrThr: 2.446 ± 0.211
3.123TyrVal: 3.123 ± 0.238
0.79TyrTrp: 0.79 ± 0.116
2.032TyrTyr: 2.032 ± 0.226
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 166 proteins (53151 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski