Escherichia coli (strain K12)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia coli

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P77263|ECPE_ECOLI Probable fimbrial chaperone EcpE OS=Escherichia coli (strain K12) OX=83333 GN=ecpE PE=3 SV=2
MM1 pKa = 7.37HH2 pKa = 6.88SWKK5 pKa = 10.29KK6 pKa = 10.49KK7 pKa = 9.88LVVSQLALACTLAITSQANAANYY30 pKa = 7.61DD31 pKa = 3.01TWTYY35 pKa = 10.78IDD37 pKa = 4.17NPVTALDD44 pKa = 3.82WDD46 pKa = 4.14HH47 pKa = 6.59MDD49 pKa = 4.13KK50 pKa = 11.31AGTVDD55 pKa = 3.75GNYY58 pKa = 9.86VNYY61 pKa = 10.78SGFVYY66 pKa = 10.79YY67 pKa = 11.15NNTNGDD73 pKa = 3.55FDD75 pKa = 3.99QSFNGDD81 pKa = 3.76TVNGTISTYY90 pKa = 10.77YY91 pKa = 10.45LNHH94 pKa = 7.64DD95 pKa = 4.38YY96 pKa = 11.55ADD98 pKa = 3.63STANQLDD105 pKa = 3.34ISNSVIHH112 pKa = 6.67GSITSMLPGGYY123 pKa = 9.26YY124 pKa = 10.45DD125 pKa = 5.9RR126 pKa = 11.84FDD128 pKa = 5.03ADD130 pKa = 3.75GNNLGGYY137 pKa = 9.86DD138 pKa = 4.6FYY140 pKa = 11.33TDD142 pKa = 3.91AVVDD146 pKa = 3.73THH148 pKa = 6.51WRR150 pKa = 11.84DD151 pKa = 3.02GDD153 pKa = 3.99VFTLNIANTTIDD165 pKa = 3.87DD166 pKa = 4.5DD167 pKa = 4.7YY168 pKa = 11.2EE169 pKa = 4.78ALYY172 pKa = 8.53FTDD175 pKa = 3.76SYY177 pKa = 11.73KK178 pKa = 11.25DD179 pKa = 3.37GDD181 pKa = 4.13VTKK184 pKa = 9.29HH185 pKa = 4.83TNEE188 pKa = 4.07TFDD191 pKa = 3.28TSEE194 pKa = 4.05GVAVNLDD201 pKa = 3.37VEE203 pKa = 4.96SNINISNNSRR213 pKa = 11.84VAGIALSQGNTYY225 pKa = 10.89NEE227 pKa = 4.58TYY229 pKa = 7.94TTEE232 pKa = 3.61SHH234 pKa = 5.81TWDD237 pKa = 3.34NNISVKK243 pKa = 10.76DD244 pKa = 3.58STVTSGSNYY253 pKa = 9.61ILDD256 pKa = 3.76SNTYY260 pKa = 9.99GKK262 pKa = 8.47TGHH265 pKa = 6.7FGNSDD270 pKa = 3.73EE271 pKa = 4.67PSDD274 pKa = 3.87YY275 pKa = 11.32AGPGDD280 pKa = 3.52VAMSFTASGSDD291 pKa = 3.35YY292 pKa = 11.64AMKK295 pKa = 11.03NNVFLSNSTLMGDD308 pKa = 3.89VAFTSTWNSNFDD320 pKa = 4.29PNGHH324 pKa = 7.08DD325 pKa = 3.76SNGDD329 pKa = 3.44GVKK332 pKa = 9.45DD333 pKa = 3.94TNGGWTDD340 pKa = 3.46DD341 pKa = 3.73SLNVDD346 pKa = 4.39EE347 pKa = 6.58LNLTLDD353 pKa = 4.31NGSKK357 pKa = 9.66WVGQAIYY364 pKa = 10.76NVAEE368 pKa = 4.05TSAMYY373 pKa = 10.4DD374 pKa = 3.21VATNSLTPDD383 pKa = 2.86ATYY386 pKa = 11.15EE387 pKa = 4.0NNDD390 pKa = 3.16WKK392 pKa = 10.85RR393 pKa = 11.84VVDD396 pKa = 4.29DD397 pKa = 5.09KK398 pKa = 11.71VFQSGVFNVALNNGSEE414 pKa = 3.96WDD416 pKa = 3.5TTGRR420 pKa = 11.84SIVDD424 pKa = 3.31TLTVNNGSQVNVSEE438 pKa = 4.74SKK440 pKa = 9.7LTSDD444 pKa = 5.45TIDD447 pKa = 3.5LTNGSSLNIGEE458 pKa = 4.88DD459 pKa = 4.23GYY461 pKa = 11.93VDD463 pKa = 3.86TDD465 pKa = 3.34HH466 pKa = 6.86LTINSYY472 pKa = 8.68STVALTEE479 pKa = 4.2STGWGADD486 pKa = 3.35YY487 pKa = 11.23NLYY490 pKa = 11.06ANTITVTNGGVLDD503 pKa = 4.08VNVDD507 pKa = 3.57QFDD510 pKa = 4.01TEE512 pKa = 4.09AFRR515 pKa = 11.84TDD517 pKa = 3.3KK518 pKa = 11.63LEE520 pKa = 4.15LTSGNIADD528 pKa = 4.0HH529 pKa = 6.51NGNVVSGVFDD539 pKa = 3.21IHH541 pKa = 8.66SSDD544 pKa = 3.72YY545 pKa = 11.19VLNADD550 pKa = 4.92LVNDD554 pKa = 4.26RR555 pKa = 11.84TWDD558 pKa = 3.61TSKK561 pKa = 11.36SNYY564 pKa = 9.88GYY566 pKa = 10.9GIVAMNSDD574 pKa = 3.53GHH576 pKa = 5.81LTINGNGDD584 pKa = 3.17VDD586 pKa = 4.69NGTEE590 pKa = 4.24LDD592 pKa = 3.71NSSVDD597 pKa = 3.52NVVAATGNYY606 pKa = 8.96KK607 pKa = 10.02VRR609 pKa = 11.84IDD611 pKa = 3.53NATGAGAIADD621 pKa = 4.08YY622 pKa = 10.27KK623 pKa = 10.72DD624 pKa = 3.46KK625 pKa = 11.16EE626 pKa = 4.21IIYY629 pKa = 10.79VNDD632 pKa = 3.27VNSNATFSAANKK644 pKa = 9.86ADD646 pKa = 3.72LGAYY650 pKa = 7.69TYY652 pKa = 10.29QAEE655 pKa = 4.36QRR657 pKa = 11.84GNTVVLQQMEE667 pKa = 4.34LTDD670 pKa = 4.16YY671 pKa = 11.84ANMALSIPSANTNIWNLEE689 pKa = 3.6QDD691 pKa = 3.86TVGTRR696 pKa = 11.84LTNSRR701 pKa = 11.84HH702 pKa = 4.84GLADD706 pKa = 3.5NGGAWVSYY714 pKa = 10.43FGGNFNGDD722 pKa = 3.55NGTINYY728 pKa = 8.82DD729 pKa = 3.13QDD731 pKa = 3.61VNGIMVGVDD740 pKa = 2.97TKK742 pKa = 11.02IDD744 pKa = 3.49GNNAKK749 pKa = 9.7WIVGAAAGFAKK760 pKa = 10.62GDD762 pKa = 3.55MNDD765 pKa = 3.31RR766 pKa = 11.84SGQVDD771 pKa = 3.54QDD773 pKa = 3.63SQTAYY778 pKa = 9.18IYY780 pKa = 11.0SSAHH784 pKa = 5.1FANNVFVDD792 pKa = 4.71GSLSYY797 pKa = 11.41SHH799 pKa = 7.31FNNDD803 pKa = 3.89LSATMSNGTYY813 pKa = 10.5VDD815 pKa = 3.75GSTNSDD821 pKa = 2.36AWGFGLKK828 pKa = 10.12AGYY831 pKa = 9.72DD832 pKa = 3.82FKK834 pKa = 11.38LGDD837 pKa = 3.77AGYY840 pKa = 7.83VTPYY844 pKa = 10.65GSVSGLFQSGDD855 pKa = 3.79DD856 pKa = 3.89YY857 pKa = 11.83QLSNDD862 pKa = 3.96MKK864 pKa = 11.23VDD866 pKa = 3.62GQSYY870 pKa = 10.64DD871 pKa = 3.02SMRR874 pKa = 11.84YY875 pKa = 8.94EE876 pKa = 4.34LGVDD880 pKa = 3.21AGYY883 pKa = 8.53TFTYY887 pKa = 10.6SEE889 pKa = 4.47DD890 pKa = 3.66QALTPYY896 pKa = 10.37FKK898 pKa = 10.64LAYY901 pKa = 10.52VYY903 pKa = 11.0DD904 pKa = 4.62DD905 pKa = 4.36SNNDD909 pKa = 2.94NDD911 pKa = 4.18VNGDD915 pKa = 3.89SIDD918 pKa = 3.64NGTEE922 pKa = 3.72GSAVRR927 pKa = 11.84VGLGTQFSFTKK938 pKa = 10.49NFSAYY943 pKa = 9.3TDD945 pKa = 3.45ANYY948 pKa = 10.81LGGGDD953 pKa = 4.6VDD955 pKa = 4.32QDD957 pKa = 2.67WSANVGVKK965 pKa = 7.43YY966 pKa = 8.84TWW968 pKa = 3.09

Molecular weight: 104.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0A8K5|YAEP_ECOLI UPF0253 protein YaeP OS=Escherichia coli (strain K12) OX=83333 GN=yaeP PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4392

58

4450

1363428

8

2358

306.4

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.503 ± 0.048

1.162 ± 0.014

5.147 ± 0.026

5.77 ± 0.038

3.887 ± 0.026

7.36 ± 0.036

2.267 ± 0.022

6.011 ± 0.035

4.414 ± 0.034

10.671 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.828 ± 0.017

3.941 ± 0.029

4.428 ± 0.029

4.446 ± 0.03

5.532 ± 0.034

5.8 ± 0.03

5.389 ± 0.029

7.071 ± 0.028

1.526 ± 0.016

2.845 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski