Amino acid dipepetide frequency for Prevotella sp. tc2-28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.059AlaAla: 6.059 ± 0.088
1.107AlaCys: 1.107 ± 0.033
4.661AlaAsp: 4.661 ± 0.079
4.756AlaGlu: 4.756 ± 0.079
3.075AlaPhe: 3.075 ± 0.06
4.971AlaGly: 4.971 ± 0.076
1.431AlaHis: 1.431 ± 0.04
4.928AlaIle: 4.928 ± 0.084
4.46AlaLys: 4.46 ± 0.078
6.674AlaLeu: 6.674 ± 0.095
2.151AlaMet: 2.151 ± 0.052
3.298AlaAsn: 3.298 ± 0.059
2.397AlaPro: 2.397 ± 0.051
3.212AlaGln: 3.212 ± 0.062
3.193AlaArg: 3.193 ± 0.063
4.06AlaSer: 4.06 ± 0.065
4.238AlaThr: 4.238 ± 0.074
5.029AlaVal: 5.029 ± 0.071
0.951AlaTrp: 0.951 ± 0.034
2.946AlaTyr: 2.946 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.895CysAla: 0.895 ± 0.031
0.236CysCys: 0.236 ± 0.015
0.767CysAsp: 0.767 ± 0.03
0.762CysGlu: 0.762 ± 0.027
0.578CysPhe: 0.578 ± 0.026
1.221CysGly: 1.221 ± 0.036
0.318CysHis: 0.318 ± 0.018
0.953CysIle: 0.953 ± 0.033
0.669CysLys: 0.669 ± 0.028
1.135CysLeu: 1.135 ± 0.032
0.355CysMet: 0.355 ± 0.018
0.63CysAsn: 0.63 ± 0.025
0.583CysPro: 0.583 ± 0.029
0.528CysGln: 0.528 ± 0.026
0.624CysArg: 0.624 ± 0.024
0.795CysSer: 0.795 ± 0.028
0.736CysThr: 0.736 ± 0.029
0.853CysVal: 0.853 ± 0.028
0.206CysTrp: 0.206 ± 0.016
0.624CysTyr: 0.624 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.536AspAla: 4.536 ± 0.068
0.746AspCys: 0.746 ± 0.03
3.427AspAsp: 3.427 ± 0.067
4.313AspGlu: 4.313 ± 0.091
2.951AspPhe: 2.951 ± 0.057
4.652AspGly: 4.652 ± 0.085
1.147AspHis: 1.147 ± 0.035
4.222AspIle: 4.222 ± 0.067
3.646AspLys: 3.646 ± 0.066
4.527AspLeu: 4.527 ± 0.071
1.679AspMet: 1.679 ± 0.047
2.827AspAsn: 2.827 ± 0.053
1.974AspPro: 1.974 ± 0.046
1.623AspGln: 1.623 ± 0.039
2.511AspArg: 2.511 ± 0.054
3.103AspSer: 3.103 ± 0.071
2.828AspThr: 2.828 ± 0.05
4.044AspVal: 4.044 ± 0.06
0.92AspTrp: 0.92 ± 0.031
3.105AspTyr: 3.105 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
4.942GluAla: 4.942 ± 0.08
0.733GluCys: 0.733 ± 0.03
3.321GluAsp: 3.321 ± 0.062
4.589GluGlu: 4.589 ± 0.078
2.238GluPhe: 2.238 ± 0.044
4.38GluGly: 4.38 ± 0.064
1.5GluHis: 1.5 ± 0.041
3.858GluIle: 3.858 ± 0.07
4.483GluLys: 4.483 ± 0.076
5.603GluLeu: 5.603 ± 0.086
2.088GluMet: 2.088 ± 0.05
2.937GluAsn: 2.937 ± 0.057
1.866GluPro: 1.866 ± 0.043
3.166GluGln: 3.166 ± 0.056
3.478GluArg: 3.478 ± 0.066
2.852GluSer: 2.852 ± 0.046
3.157GluThr: 3.157 ± 0.056
4.129GluVal: 4.129 ± 0.072
0.874GluTrp: 0.874 ± 0.028
2.563GluTyr: 2.563 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
2.928PheAla: 2.928 ± 0.057
0.759PheCys: 0.759 ± 0.032
2.846PheAsp: 2.846 ± 0.052
2.278PheGlu: 2.278 ± 0.044
1.987PhePhe: 1.987 ± 0.057
3.243PheGly: 3.243 ± 0.065
0.936PheHis: 0.936 ± 0.031
2.736PheIle: 2.736 ± 0.051
2.251PheLys: 2.251 ± 0.056
3.557PheLeu: 3.557 ± 0.067
1.197PheMet: 1.197 ± 0.033
2.082PheAsn: 2.082 ± 0.052
1.541PhePro: 1.541 ± 0.035
1.3PheGln: 1.3 ± 0.035
2.068PheArg: 2.068 ± 0.043
2.914PheSer: 2.914 ± 0.056
2.683PheThr: 2.683 ± 0.049
2.908PheVal: 2.908 ± 0.051
0.58PheTrp: 0.58 ± 0.025
1.764PheTyr: 1.764 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.63GlyAla: 4.63 ± 0.081
1.053GlyCys: 1.053 ± 0.04
3.874GlyAsp: 3.874 ± 0.074
4.079GlyGlu: 4.079 ± 0.064
3.091GlyPhe: 3.091 ± 0.052
5.205GlyGly: 5.205 ± 0.133
1.548GlyHis: 1.548 ± 0.046
5.183GlyIle: 5.183 ± 0.08
4.974GlyLys: 4.974 ± 0.076
5.908GlyLeu: 5.908 ± 0.077
2.272GlyMet: 2.272 ± 0.05
3.486GlyAsn: 3.486 ± 0.082
1.441GlyPro: 1.441 ± 0.041
2.546GlyGln: 2.546 ± 0.054
3.336GlyArg: 3.336 ± 0.055
3.945GlySer: 3.945 ± 0.081
4.367GlyThr: 4.367 ± 0.092
4.848GlyVal: 4.848 ± 0.083
1.068GlyTrp: 1.068 ± 0.043
3.236GlyTyr: 3.236 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.293HisAla: 1.293 ± 0.034
0.331HisCys: 0.331 ± 0.02
1.199HisAsp: 1.199 ± 0.034
1.221HisGlu: 1.221 ± 0.037
1.111HisPhe: 1.111 ± 0.031
1.433HisGly: 1.433 ± 0.038
0.646HisHis: 0.646 ± 0.03
1.455HisIle: 1.455 ± 0.042
1.031HisLys: 1.031 ± 0.036
2.037HisLeu: 2.037 ± 0.053
0.477HisMet: 0.477 ± 0.022
0.966HisAsn: 0.966 ± 0.033
1.134HisPro: 1.134 ± 0.039
0.913HisGln: 0.913 ± 0.03
1.115HisArg: 1.115 ± 0.033
1.161HisSer: 1.161 ± 0.031
1.12HisThr: 1.12 ± 0.036
1.344HisVal: 1.344 ± 0.041
0.295HisTrp: 0.295 ± 0.016
1.078HisTyr: 1.078 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.095IleAla: 5.095 ± 0.081
1.03IleCys: 1.03 ± 0.033
4.382IleAsp: 4.382 ± 0.072
4.112IleGlu: 4.112 ± 0.07
2.407IlePhe: 2.407 ± 0.055
4.724IleGly: 4.724 ± 0.072
1.26IleHis: 1.26 ± 0.042
4.431IleIle: 4.431 ± 0.092
3.788IleLys: 3.788 ± 0.063
5.052IleLeu: 5.052 ± 0.082
1.586IleMet: 1.586 ± 0.041
3.176IleAsn: 3.176 ± 0.06
2.893IlePro: 2.893 ± 0.057
2.221IleGln: 2.221 ± 0.054
3.217IleArg: 3.217 ± 0.058
4.135IleSer: 4.135 ± 0.061
3.976IleThr: 3.976 ± 0.078
4.412IleVal: 4.412 ± 0.075
0.744IleTrp: 0.744 ± 0.028
2.479IleTyr: 2.479 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
5.067LysAla: 5.067 ± 0.078
0.55LysCys: 0.55 ± 0.023
3.959LysAsp: 3.959 ± 0.065
4.756LysGlu: 4.756 ± 0.077
2.049LysPhe: 2.049 ± 0.043
4.395LysGly: 4.395 ± 0.065
1.213LysHis: 1.213 ± 0.035
3.721LysIle: 3.721 ± 0.063
4.752LysLys: 4.752 ± 0.085
4.777LysLeu: 4.777 ± 0.077
2.284LysMet: 2.284 ± 0.054
3.126LysAsn: 3.126 ± 0.057
2.287LysPro: 2.287 ± 0.048
2.738LysGln: 2.738 ± 0.057
2.99LysArg: 2.99 ± 0.058
3.135LysSer: 3.135 ± 0.06
3.658LysThr: 3.658 ± 0.06
4.178LysVal: 4.178 ± 0.065
0.837LysTrp: 0.837 ± 0.029
2.702LysTyr: 2.702 ± 0.051
0.0LysXaa: 0.0 ± 0.0
Leu
6.346LeuAla: 6.346 ± 0.089
1.327LeuCys: 1.327 ± 0.032
4.718LeuAsp: 4.718 ± 0.067
4.692LeuGlu: 4.692 ± 0.074
3.911LeuPhe: 3.911 ± 0.072
5.515LeuGly: 5.515 ± 0.08
1.864LeuHis: 1.864 ± 0.041
4.991LeuIle: 4.991 ± 0.09
5.603LeuLys: 5.603 ± 0.082
8.19LeuLeu: 8.19 ± 0.137
2.891LeuMet: 2.891 ± 0.053
4.221LeuAsn: 4.221 ± 0.071
3.737LeuPro: 3.737 ± 0.067
3.507LeuGln: 3.507 ± 0.074
4.523LeuArg: 4.523 ± 0.066
5.89LeuSer: 5.89 ± 0.089
5.717LeuThr: 5.717 ± 0.085
5.245LeuVal: 5.245 ± 0.084
1.114LeuTrp: 1.114 ± 0.038
3.405LeuTyr: 3.405 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.599MetAla: 2.599 ± 0.054
0.289MetCys: 0.289 ± 0.015
1.602MetAsp: 1.602 ± 0.041
1.868MetGlu: 1.868 ± 0.041
1.026MetPhe: 1.026 ± 0.032
1.998MetGly: 1.998 ± 0.045
0.593MetHis: 0.593 ± 0.024
1.566MetIle: 1.566 ± 0.041
2.617MetLys: 2.617 ± 0.053
2.787MetLeu: 2.787 ± 0.054
1.145MetMet: 1.145 ± 0.039
1.563MetAsn: 1.563 ± 0.04
1.287MetPro: 1.287 ± 0.038
1.326MetGln: 1.326 ± 0.036
1.543MetArg: 1.543 ± 0.042
1.704MetSer: 1.704 ± 0.041
1.962MetThr: 1.962 ± 0.045
1.839MetVal: 1.839 ± 0.041
0.273MetTrp: 0.273 ± 0.016
0.834MetTyr: 0.834 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.563AsnAla: 3.563 ± 0.066
0.578AsnCys: 0.578 ± 0.029
2.797AsnAsp: 2.797 ± 0.059
2.766AsnGlu: 2.766 ± 0.052
1.884AsnPhe: 1.884 ± 0.042
4.107AsnGly: 4.107 ± 0.081
1.065AsnHis: 1.065 ± 0.03
3.427AsnIle: 3.427 ± 0.066
2.785AsnLys: 2.785 ± 0.062
3.787AsnLeu: 3.787 ± 0.06
1.298AsnMet: 1.298 ± 0.037
2.504AsnAsn: 2.504 ± 0.061
2.519AsnPro: 2.519 ± 0.053
1.741AsnGln: 1.741 ± 0.042
2.231AsnArg: 2.231 ± 0.051
2.495AsnSer: 2.495 ± 0.047
2.647AsnThr: 2.647 ± 0.062
3.186AsnVal: 3.186 ± 0.065
0.623AsnTrp: 0.623 ± 0.025
2.261AsnTyr: 2.261 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
2.681ProAla: 2.681 ± 0.054
0.405ProCys: 0.405 ± 0.022
2.553ProAsp: 2.553 ± 0.051
2.978ProGlu: 2.978 ± 0.053
1.696ProPhe: 1.696 ± 0.035
2.161ProGly: 2.161 ± 0.049
0.787ProHis: 0.787 ± 0.029
2.221ProIle: 2.221 ± 0.049
2.109ProLys: 2.109 ± 0.043
3.211ProLeu: 3.211 ± 0.067
1.107ProMet: 1.107 ± 0.036
1.751ProAsn: 1.751 ± 0.044
0.716ProPro: 0.716 ± 0.027
1.621ProGln: 1.621 ± 0.047
1.419ProArg: 1.419 ± 0.044
2.146ProSer: 2.146 ± 0.046
2.246ProThr: 2.246 ± 0.053
2.866ProVal: 2.866 ± 0.055
0.503ProTrp: 0.503 ± 0.021
1.764ProTyr: 1.764 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.753GlnAla: 2.753 ± 0.057
0.433GlnCys: 0.433 ± 0.02
1.764GlnAsp: 1.764 ± 0.04
2.472GlnGlu: 2.472 ± 0.061
1.588GlnPhe: 1.588 ± 0.036
2.516GlnGly: 2.516 ± 0.053
0.954GlnHis: 0.954 ± 0.032
2.321GlnIle: 2.321 ± 0.052
2.735GlnLys: 2.735 ± 0.052
4.067GlnLeu: 4.067 ± 0.066
1.502GlnMet: 1.502 ± 0.042
1.74GlnAsn: 1.74 ± 0.041
1.54GlnPro: 1.54 ± 0.04
2.532GlnGln: 2.532 ± 0.07
2.103GlnArg: 2.103 ± 0.043
1.992GlnSer: 1.992 ± 0.046
2.311GlnThr: 2.311 ± 0.054
2.419GlnVal: 2.419 ± 0.054
0.621GlnTrp: 0.621 ± 0.025
1.668GlnTyr: 1.668 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
2.863ArgAla: 2.863 ± 0.052
0.545ArgCys: 0.545 ± 0.023
2.489ArgAsp: 2.489 ± 0.05
3.158ArgGlu: 3.158 ± 0.06
2.238ArgPhe: 2.238 ± 0.054
2.653ArgGly: 2.653 ± 0.05
1.149ArgHis: 1.149 ± 0.037
3.414ArgIle: 3.414 ± 0.056
3.114ArgLys: 3.114 ± 0.066
4.693ArgLeu: 4.693 ± 0.086
1.668ArgMet: 1.668 ± 0.039
2.355ArgAsn: 2.355 ± 0.049
1.645ArgPro: 1.645 ± 0.038
2.507ArgGln: 2.507 ± 0.058
2.608ArgArg: 2.608 ± 0.066
2.303ArgSer: 2.303 ± 0.047
2.415ArgThr: 2.415 ± 0.046
2.775ArgVal: 2.775 ± 0.045
0.796ArgTrp: 0.796 ± 0.026
2.31ArgTyr: 2.31 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
4.075SerAla: 4.075 ± 0.064
0.825SerCys: 0.825 ± 0.031
3.428SerAsp: 3.428 ± 0.061
3.416SerGlu: 3.416 ± 0.05
2.714SerPhe: 2.714 ± 0.062
4.152SerGly: 4.152 ± 0.082
1.246SerHis: 1.246 ± 0.036
3.764SerIle: 3.764 ± 0.07
3.261SerLys: 3.261 ± 0.06
5.241SerLeu: 5.241 ± 0.067
1.544SerMet: 1.544 ± 0.038
2.513SerAsn: 2.513 ± 0.061
2.142SerPro: 2.142 ± 0.047
2.144SerGln: 2.144 ± 0.043
2.521SerArg: 2.521 ± 0.055
3.475SerSer: 3.475 ± 0.075
3.15SerThr: 3.15 ± 0.065
4.014SerVal: 4.014 ± 0.069
0.795SerTrp: 0.795 ± 0.031
2.477SerTyr: 2.477 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.431ThrAla: 4.431 ± 0.08
0.635ThrCys: 0.635 ± 0.026
3.496ThrAsp: 3.496 ± 0.057
3.257ThrGlu: 3.257 ± 0.056
2.707ThrPhe: 2.707 ± 0.052
4.352ThrGly: 4.352 ± 0.085
1.187ThrHis: 1.187 ± 0.034
4.291ThrIle: 4.291 ± 0.086
3.155ThrLys: 3.155 ± 0.063
5.557ThrLeu: 5.557 ± 0.08
1.57ThrMet: 1.57 ± 0.043
2.47ThrAsn: 2.47 ± 0.055
2.667ThrPro: 2.667 ± 0.058
1.903ThrGln: 1.903 ± 0.041
2.246ThrArg: 2.246 ± 0.043
3.358ThrSer: 3.358 ± 0.068
3.609ThrThr: 3.609 ± 0.081
4.081ThrVal: 4.081 ± 0.078
0.797ThrTrp: 0.797 ± 0.033
2.456ThrTyr: 2.456 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
4.907ValAla: 4.907 ± 0.067
1.069ValCys: 1.069 ± 0.032
3.901ValAsp: 3.901 ± 0.064
4.111ValGlu: 4.111 ± 0.069
2.768ValPhe: 2.768 ± 0.058
4.341ValGly: 4.341 ± 0.08
1.125ValHis: 1.125 ± 0.03
4.287ValIle: 4.287 ± 0.072
4.427ValLys: 4.427 ± 0.074
5.735ValLeu: 5.735 ± 0.086
1.976ValMet: 1.976 ± 0.039
3.407ValAsn: 3.407 ± 0.06
2.517ValPro: 2.517 ± 0.053
2.043ValGln: 2.043 ± 0.051
3.129ValArg: 3.129 ± 0.059
4.261ValSer: 4.261 ± 0.07
4.117ValThr: 4.117 ± 0.092
4.867ValVal: 4.867 ± 0.077
0.836ValTrp: 0.836 ± 0.031
2.615ValTyr: 2.615 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.912TrpAla: 0.912 ± 0.031
0.213TrpCys: 0.213 ± 0.016
0.781TrpAsp: 0.781 ± 0.03
0.725TrpGlu: 0.725 ± 0.027
0.577TrpPhe: 0.577 ± 0.028
0.991TrpGly: 0.991 ± 0.036
0.338TrpHis: 0.338 ± 0.02
0.786TrpIle: 0.786 ± 0.029
0.858TrpLys: 0.858 ± 0.034
1.397TrpLeu: 1.397 ± 0.042
0.51TrpMet: 0.51 ± 0.022
0.792TrpAsn: 0.792 ± 0.029
0.37TrpPro: 0.37 ± 0.017
0.697TrpGln: 0.697 ± 0.028
0.659TrpArg: 0.659 ± 0.026
0.787TrpSer: 0.787 ± 0.028
0.74TrpThr: 0.74 ± 0.029
0.728TrpVal: 0.728 ± 0.03
0.262TrpTrp: 0.262 ± 0.017
0.585TrpTyr: 0.585 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.023TyrAla: 3.023 ± 0.051
0.605TyrCys: 0.605 ± 0.024
2.819TyrAsp: 2.819 ± 0.06
2.448TyrGlu: 2.448 ± 0.05
1.961TyrPhe: 1.961 ± 0.043
3.078TyrGly: 3.078 ± 0.061
1.052TyrHis: 1.052 ± 0.034
2.564TyrIle: 2.564 ± 0.047
2.457TyrLys: 2.457 ± 0.051
3.471TyrLeu: 3.471 ± 0.064
1.12TyrMet: 1.12 ± 0.032
2.419TyrAsn: 2.419 ± 0.055
1.685TyrPro: 1.685 ± 0.042
1.765TyrGln: 1.765 ± 0.047
2.184TyrArg: 2.184 ± 0.052
2.387TyrSer: 2.387 ± 0.063
2.546TyrThr: 2.546 ± 0.06
2.676TyrVal: 2.676 ± 0.061
0.612TyrTrp: 0.612 ± 0.029
2.195TyrTyr: 2.195 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2895 proteins (1038832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski