Amino acid dipepetide frequency for Bacillus sp. V59.32b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.2AlaAla: 6.2 ± 0.115
0.561AlaCys: 0.561 ± 0.024
3.599AlaAsp: 3.599 ± 0.058
4.998AlaGlu: 4.998 ± 0.095
3.287AlaPhe: 3.287 ± 0.068
5.822AlaGly: 5.822 ± 0.093
1.274AlaHis: 1.274 ± 0.04
5.863AlaIle: 5.863 ± 0.084
4.878AlaLys: 4.878 ± 0.075
7.182AlaLeu: 7.182 ± 0.094
2.042AlaMet: 2.042 ± 0.046
2.728AlaAsn: 2.728 ± 0.06
2.097AlaPro: 2.097 ± 0.056
2.136AlaGln: 2.136 ± 0.049
2.75AlaArg: 2.75 ± 0.059
4.182AlaSer: 4.182 ± 0.075
3.473AlaThr: 3.473 ± 0.063
5.62AlaVal: 5.62 ± 0.086
0.566AlaTrp: 0.566 ± 0.024
2.348AlaTyr: 2.348 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.448CysAla: 0.448 ± 0.022
0.093CysCys: 0.093 ± 0.01
0.396CysAsp: 0.396 ± 0.019
0.457CysGlu: 0.457 ± 0.021
0.316CysPhe: 0.316 ± 0.017
0.692CysGly: 0.692 ± 0.03
0.215CysHis: 0.215 ± 0.015
0.504CysIle: 0.504 ± 0.024
0.397CysLys: 0.397 ± 0.021
0.646CysLeu: 0.646 ± 0.026
0.176CysMet: 0.176 ± 0.014
0.292CysAsn: 0.292 ± 0.018
0.356CysPro: 0.356 ± 0.021
0.211CysGln: 0.211 ± 0.015
0.279CysArg: 0.279 ± 0.016
0.468CysSer: 0.468 ± 0.024
0.366CysThr: 0.366 ± 0.019
0.413CysVal: 0.413 ± 0.023
0.067CysTrp: 0.067 ± 0.008
0.252CysTyr: 0.252 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.258AspAla: 3.258 ± 0.072
0.404AspCys: 0.404 ± 0.019
2.583AspAsp: 2.583 ± 0.06
4.544AspGlu: 4.544 ± 0.068
2.527AspPhe: 2.527 ± 0.052
3.532AspGly: 3.532 ± 0.062
1.148AspHis: 1.148 ± 0.033
4.304AspIle: 4.304 ± 0.061
3.329AspLys: 3.329 ± 0.059
5.074AspLeu: 5.074 ± 0.076
1.332AspMet: 1.332 ± 0.035
1.901AspAsn: 1.901 ± 0.047
2.03AspPro: 2.03 ± 0.05
1.852AspGln: 1.852 ± 0.042
2.341AspArg: 2.341 ± 0.05
2.829AspSer: 2.829 ± 0.053
2.494AspThr: 2.494 ± 0.049
3.622AspVal: 3.622 ± 0.07
0.65AspTrp: 0.65 ± 0.025
2.183AspTyr: 2.183 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.646GluAla: 5.646 ± 0.09
0.364GluCys: 0.364 ± 0.023
3.881GluAsp: 3.881 ± 0.076
7.119GluGlu: 7.119 ± 0.119
2.662GluPhe: 2.662 ± 0.05
4.359GluGly: 4.359 ± 0.068
1.467GluHis: 1.467 ± 0.043
6.139GluIle: 6.139 ± 0.093
6.786GluLys: 6.786 ± 0.087
7.244GluLeu: 7.244 ± 0.097
2.503GluMet: 2.503 ± 0.053
3.811GluAsn: 3.811 ± 0.068
1.937GluPro: 1.937 ± 0.04
3.112GluGln: 3.112 ± 0.053
3.629GluArg: 3.629 ± 0.06
3.754GluSer: 3.754 ± 0.063
4.094GluThr: 4.094 ± 0.059
5.0GluVal: 5.0 ± 0.082
0.775GluTrp: 0.775 ± 0.027
2.337GluTyr: 2.337 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.086PheAla: 3.086 ± 0.059
0.383PheCys: 0.383 ± 0.02
2.34PheAsp: 2.34 ± 0.051
2.947PheGlu: 2.947 ± 0.054
2.358PhePhe: 2.358 ± 0.062
3.375PheGly: 3.375 ± 0.065
1.017PheHis: 1.017 ± 0.031
3.738PheIle: 3.738 ± 0.062
2.418PheLys: 2.418 ± 0.041
4.478PheLeu: 4.478 ± 0.081
1.18PheMet: 1.18 ± 0.034
1.842PheAsn: 1.842 ± 0.038
1.729PhePro: 1.729 ± 0.042
1.596PheGln: 1.596 ± 0.044
1.546PheArg: 1.546 ± 0.043
3.271PheSer: 3.271 ± 0.066
2.505PheThr: 2.505 ± 0.053
2.963PheVal: 2.963 ± 0.056
0.501PheTrp: 0.501 ± 0.021
1.639PheTyr: 1.639 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.851GlyAla: 4.851 ± 0.083
0.664GlyCys: 0.664 ± 0.027
3.382GlyAsp: 3.382 ± 0.062
4.698GlyGlu: 4.698 ± 0.077
3.532GlyPhe: 3.532 ± 0.065
4.95GlyGly: 4.95 ± 0.078
1.426GlyHis: 1.426 ± 0.038
6.24GlyIle: 6.24 ± 0.097
5.375GlyLys: 5.375 ± 0.074
6.61GlyLeu: 6.61 ± 0.083
2.133GlyMet: 2.133 ± 0.052
2.931GlyAsn: 2.931 ± 0.061
1.783GlyPro: 1.783 ± 0.042
2.25GlyGln: 2.25 ± 0.048
2.844GlyArg: 2.844 ± 0.052
4.246GlySer: 4.246 ± 0.073
4.109GlyThr: 4.109 ± 0.068
4.908GlyVal: 4.908 ± 0.079
0.824GlyTrp: 0.824 ± 0.029
2.858GlyTyr: 2.858 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.255HisAla: 1.255 ± 0.035
0.197HisCys: 0.197 ± 0.013
1.065HisAsp: 1.065 ± 0.033
1.414HisGlu: 1.414 ± 0.035
1.046HisPhe: 1.046 ± 0.033
1.429HisGly: 1.429 ± 0.035
0.631HisHis: 0.631 ± 0.029
1.536HisIle: 1.536 ± 0.041
1.068HisLys: 1.068 ± 0.031
2.034HisLeu: 2.034 ± 0.044
0.496HisMet: 0.496 ± 0.021
0.788HisAsn: 0.788 ± 0.028
1.113HisPro: 1.113 ± 0.033
0.76HisGln: 0.76 ± 0.026
0.918HisArg: 0.918 ± 0.036
1.262HisSer: 1.262 ± 0.035
1.054HisThr: 1.054 ± 0.034
1.424HisVal: 1.424 ± 0.036
0.202HisTrp: 0.202 ± 0.015
0.796HisTyr: 0.796 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.004IleAla: 6.004 ± 0.088
0.636IleCys: 0.636 ± 0.023
4.349IleAsp: 4.349 ± 0.071
5.827IleGlu: 5.827 ± 0.085
3.251IlePhe: 3.251 ± 0.071
6.005IleGly: 6.005 ± 0.091
1.659IleHis: 1.659 ± 0.04
6.065IleIle: 6.065 ± 0.091
4.696IleLys: 4.696 ± 0.07
7.186IleLeu: 7.186 ± 0.097
1.899IleMet: 1.899 ± 0.046
3.385IleAsn: 3.385 ± 0.068
3.347IlePro: 3.347 ± 0.056
2.869IleGln: 2.869 ± 0.058
3.21IleArg: 3.21 ± 0.061
5.323IleSer: 5.323 ± 0.075
4.402IleThr: 4.402 ± 0.064
5.476IleVal: 5.476 ± 0.082
0.618IleTrp: 0.618 ± 0.025
2.387IleTyr: 2.387 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.956LysAla: 4.956 ± 0.074
0.325LysCys: 0.325 ± 0.017
3.892LysAsp: 3.892 ± 0.061
6.845LysGlu: 6.845 ± 0.091
1.971LysPhe: 1.971 ± 0.041
4.783LysGly: 4.783 ± 0.069
1.344LysHis: 1.344 ± 0.041
4.923LysIle: 4.923 ± 0.066
5.908LysLys: 5.908 ± 0.088
5.942LysLeu: 5.942 ± 0.076
2.245LysMet: 2.245 ± 0.044
3.346LysAsn: 3.346 ± 0.058
2.425LysPro: 2.425 ± 0.048
3.086LysGln: 3.086 ± 0.059
3.262LysArg: 3.262 ± 0.053
3.599LysSer: 3.599 ± 0.069
3.751LysThr: 3.751 ± 0.065
4.63LysVal: 4.63 ± 0.075
0.787LysTrp: 0.787 ± 0.028
2.156LysTyr: 2.156 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
7.353LeuAla: 7.353 ± 0.101
0.607LeuCys: 0.607 ± 0.025
4.828LeuAsp: 4.828 ± 0.074
6.672LeuGlu: 6.672 ± 0.08
4.658LeuPhe: 4.658 ± 0.09
6.508LeuGly: 6.508 ± 0.093
1.933LeuHis: 1.933 ± 0.045
7.087LeuIle: 7.087 ± 0.091
6.698LeuLys: 6.698 ± 0.08
9.815LeuLeu: 9.815 ± 0.122
2.46LeuMet: 2.46 ± 0.053
4.191LeuAsn: 4.191 ± 0.057
3.943LeuPro: 3.943 ± 0.076
3.527LeuGln: 3.527 ± 0.055
3.635LeuArg: 3.635 ± 0.06
6.748LeuSer: 6.748 ± 0.082
5.515LeuThr: 5.515 ± 0.069
6.155LeuVal: 6.155 ± 0.101
0.77LeuTrp: 0.77 ± 0.03
3.049LeuTyr: 3.049 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.18MetAla: 2.18 ± 0.049
0.14MetCys: 0.14 ± 0.01
1.636MetAsp: 1.636 ± 0.035
2.094MetGlu: 2.094 ± 0.049
1.069MetPhe: 1.069 ± 0.034
1.806MetGly: 1.806 ± 0.042
0.407MetHis: 0.407 ± 0.022
2.173MetIle: 2.173 ± 0.046
2.486MetLys: 2.486 ± 0.048
2.589MetLeu: 2.589 ± 0.058
0.815MetMet: 0.815 ± 0.03
1.523MetAsn: 1.523 ± 0.043
1.027MetPro: 1.027 ± 0.029
0.878MetGln: 0.878 ± 0.031
1.058MetArg: 1.058 ± 0.03
1.619MetSer: 1.619 ± 0.038
1.56MetThr: 1.56 ± 0.039
1.74MetVal: 1.74 ± 0.036
0.164MetTrp: 0.164 ± 0.014
0.72MetTyr: 0.72 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.791AsnAla: 2.791 ± 0.054
0.288AsnCys: 0.288 ± 0.017
2.289AsnAsp: 2.289 ± 0.052
3.58AsnGlu: 3.58 ± 0.061
1.609AsnPhe: 1.609 ± 0.043
3.462AsnGly: 3.462 ± 0.063
1.095AsnHis: 1.095 ± 0.031
3.301AsnIle: 3.301 ± 0.064
2.998AsnLys: 2.998 ± 0.058
3.805AsnLeu: 3.805 ± 0.062
1.192AsnMet: 1.192 ± 0.031
1.926AsnAsn: 1.926 ± 0.045
2.255AsnPro: 2.255 ± 0.05
1.866AsnGln: 1.866 ± 0.042
2.125AsnArg: 2.125 ± 0.044
2.319AsnSer: 2.319 ± 0.055
2.081AsnThr: 2.081 ± 0.038
2.939AsnVal: 2.939 ± 0.054
0.487AsnTrp: 0.487 ± 0.021
1.448AsnTyr: 1.448 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
2.563ProAla: 2.563 ± 0.058
0.228ProCys: 0.228 ± 0.016
2.239ProAsp: 2.239 ± 0.048
3.229ProGlu: 3.229 ± 0.068
2.018ProPhe: 2.018 ± 0.042
2.597ProGly: 2.597 ± 0.052
0.758ProHis: 0.758 ± 0.024
2.765ProIle: 2.765 ± 0.056
2.21ProLys: 2.21 ± 0.045
3.495ProLeu: 3.495 ± 0.067
0.786ProMet: 0.786 ± 0.03
1.607ProAsn: 1.607 ± 0.04
1.051ProPro: 1.051 ± 0.03
1.144ProGln: 1.144 ± 0.041
1.208ProArg: 1.208 ± 0.037
2.209ProSer: 2.209 ± 0.049
1.83ProThr: 1.83 ± 0.043
2.964ProVal: 2.964 ± 0.059
0.338ProTrp: 0.338 ± 0.018
1.313ProTyr: 1.313 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
2.739GlnAla: 2.739 ± 0.051
0.197GlnCys: 0.197 ± 0.013
1.658GlnAsp: 1.658 ± 0.046
2.815GlnGlu: 2.815 ± 0.065
1.565GlnPhe: 1.565 ± 0.039
2.176GlnGly: 2.176 ± 0.044
0.724GlnHis: 0.724 ± 0.025
2.556GlnIle: 2.556 ± 0.05
2.742GlnLys: 2.742 ± 0.053
3.588GlnLeu: 3.588 ± 0.053
1.071GlnMet: 1.071 ± 0.031
1.659GlnAsn: 1.659 ± 0.047
1.231GlnPro: 1.231 ± 0.038
1.594GlnGln: 1.594 ± 0.048
1.436GlnArg: 1.436 ± 0.039
2.122GlnSer: 2.122 ± 0.053
1.999GlnThr: 1.999 ± 0.045
2.274GlnVal: 2.274 ± 0.049
0.359GlnTrp: 0.359 ± 0.021
1.311GlnTyr: 1.311 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.495ArgAla: 2.495 ± 0.051
0.271ArgCys: 0.271 ± 0.015
2.153ArgAsp: 2.153 ± 0.044
3.409ArgGlu: 3.409 ± 0.06
2.016ArgPhe: 2.016 ± 0.049
2.393ArgGly: 2.393 ± 0.052
0.807ArgHis: 0.807 ± 0.027
3.182ArgIle: 3.182 ± 0.057
3.28ArgLys: 3.28 ± 0.058
4.092ArgLeu: 4.092 ± 0.065
1.305ArgMet: 1.305 ± 0.036
1.991ArgAsn: 1.991 ± 0.043
1.416ArgPro: 1.416 ± 0.037
1.532ArgGln: 1.532 ± 0.04
1.951ArgArg: 1.951 ± 0.045
2.32ArgSer: 2.32 ± 0.047
2.055ArgThr: 2.055 ± 0.049
2.615ArgVal: 2.615 ± 0.059
0.402ArgTrp: 0.402 ± 0.022
1.518ArgTyr: 1.518 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
3.911SerAla: 3.911 ± 0.065
0.42SerCys: 0.42 ± 0.023
2.988SerAsp: 2.988 ± 0.049
4.301SerGlu: 4.301 ± 0.066
3.242SerPhe: 3.242 ± 0.058
4.648SerGly: 4.648 ± 0.073
1.239SerHis: 1.239 ± 0.033
5.05SerIle: 5.05 ± 0.084
3.865SerLys: 3.865 ± 0.068
6.249SerLeu: 6.249 ± 0.081
1.735SerMet: 1.735 ± 0.039
2.497SerAsn: 2.497 ± 0.052
2.212SerPro: 2.212 ± 0.045
1.925SerGln: 1.925 ± 0.051
2.48SerArg: 2.48 ± 0.053
4.03SerSer: 4.03 ± 0.088
3.011SerThr: 3.011 ± 0.06
4.205SerVal: 4.205 ± 0.061
0.645SerTrp: 0.645 ± 0.026
2.199SerTyr: 2.199 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.274ThrAla: 4.274 ± 0.078
0.329ThrCys: 0.329 ± 0.018
2.787ThrAsp: 2.787 ± 0.058
3.855ThrGlu: 3.855 ± 0.058
2.544ThrPhe: 2.544 ± 0.058
4.373ThrGly: 4.373 ± 0.068
0.99ThrHis: 0.99 ± 0.032
4.385ThrIle: 4.385 ± 0.064
3.307ThrLys: 3.307 ± 0.065
4.999ThrLeu: 4.999 ± 0.056
1.368ThrMet: 1.368 ± 0.042
2.334ThrAsn: 2.334 ± 0.052
2.191ThrPro: 2.191 ± 0.042
1.349ThrGln: 1.349 ± 0.036
1.833ThrArg: 1.833 ± 0.039
3.159ThrSer: 3.159 ± 0.068
2.704ThrThr: 2.704 ± 0.052
4.203ThrVal: 4.203 ± 0.065
0.518ThrTrp: 0.518 ± 0.023
1.82ThrTyr: 1.82 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
4.912ValAla: 4.912 ± 0.076
0.561ValCys: 0.561 ± 0.025
3.447ValAsp: 3.447 ± 0.055
4.678ValGlu: 4.678 ± 0.073
3.038ValPhe: 3.038 ± 0.061
4.513ValGly: 4.513 ± 0.074
1.341ValHis: 1.341 ± 0.039
5.647ValIle: 5.647 ± 0.087
4.865ValLys: 4.865 ± 0.074
6.635ValLeu: 6.635 ± 0.087
1.817ValMet: 1.817 ± 0.05
3.092ValAsn: 3.092 ± 0.053
2.71ValPro: 2.71 ± 0.053
2.261ValGln: 2.261 ± 0.05
2.723ValArg: 2.723 ± 0.052
4.707ValSer: 4.707 ± 0.076
4.138ValThr: 4.138 ± 0.065
4.694ValVal: 4.694 ± 0.072
0.59ValTrp: 0.59 ± 0.024
2.258ValTyr: 2.258 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.62TrpAla: 0.62 ± 0.028
0.065TrpCys: 0.065 ± 0.008
0.525TrpAsp: 0.525 ± 0.025
0.632TrpGlu: 0.632 ± 0.027
0.521TrpPhe: 0.521 ± 0.026
0.705TrpGly: 0.705 ± 0.032
0.227TrpHis: 0.227 ± 0.016
0.738TrpIle: 0.738 ± 0.029
0.733TrpLys: 0.733 ± 0.029
1.09TrpLeu: 1.09 ± 0.038
0.302TrpMet: 0.302 ± 0.017
0.532TrpAsn: 0.532 ± 0.025
0.263TrpPro: 0.263 ± 0.016
0.328TrpGln: 0.328 ± 0.019
0.377TrpArg: 0.377 ± 0.02
0.533TrpSer: 0.533 ± 0.021
0.484TrpThr: 0.484 ± 0.024
0.644TrpVal: 0.644 ± 0.026
0.132TrpTrp: 0.132 ± 0.011
0.315TrpTyr: 0.315 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.063TyrAla: 2.063 ± 0.039
0.31TyrCys: 0.31 ± 0.018
1.902TyrAsp: 1.902 ± 0.052
2.505TyrGlu: 2.505 ± 0.053
1.684TyrPhe: 1.684 ± 0.043
2.484TyrGly: 2.484 ± 0.049
0.784TyrHis: 0.784 ± 0.029
2.397TyrIle: 2.397 ± 0.045
2.139TyrLys: 2.139 ± 0.046
3.401TyrLeu: 3.401 ± 0.061
0.824TyrMet: 0.824 ± 0.028
1.48TyrAsn: 1.48 ± 0.038
1.438TyrPro: 1.438 ± 0.044
1.539TyrGln: 1.539 ± 0.048
1.597TyrArg: 1.597 ± 0.045
2.153TyrSer: 2.153 ± 0.054
1.697TyrThr: 1.697 ± 0.042
2.135TyrVal: 2.135 ± 0.051
0.373TyrTrp: 0.373 ± 0.02
1.283TyrTyr: 1.283 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3615 proteins (1020100 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski