Kaistia algarum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Kaistiaceae; Kaistia

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5TUP7|A0A2S5TUP7_9RHIZ Cell division coordinator CpoB OS=Kaistia algarum OX=2083279 GN=ygbF PE=3 SV=1
MM1 pKa = 7.32GVARR5 pKa = 11.84LLAATAFIAPSAALAQVQTGTCFAQALPSGGFAFSGSCQASSSSLIGLTGTGATGPNGSGADD67 pKa = 3.72LFYY70 pKa = 10.93SATDD74 pKa = 3.72GGPGATGPAFTFDD87 pKa = 4.61LLSGSQLLQQASAGNGLYY105 pKa = 10.63FSTTGGTGGIGGHH118 pKa = 6.49DD119 pKa = 3.31AVARR123 pKa = 11.84HH124 pKa = 6.11AGDD127 pKa = 3.84GGSGGDD133 pKa = 3.49GGVIIIDD140 pKa = 3.86ISATSAVSTTGDD152 pKa = 3.35NSDD155 pKa = 4.3AIYY158 pKa = 10.24AISSGGTGGQGGADD172 pKa = 3.28VDD174 pKa = 4.12GGLGDD179 pKa = 4.35GNGGPGGNGGDD190 pKa = 2.96ITVDD194 pKa = 3.0NDD196 pKa = 3.62GTLSTAGVYY205 pKa = 10.32SSAIFAHH212 pKa = 6.61SDD214 pKa = 2.99GGTGGAGAPAQSFFGNGGSGGVGGNSGADD243 pKa = 3.21VTARR247 pKa = 11.84NAGTASTSGDD257 pKa = 3.24NATAIVLQSVGGNGGDD273 pKa = 3.37GGGGQGAFYY282 pKa = 10.66SQGGNGGSGGNGEE295 pKa = 5.43DD296 pKa = 2.89IQFANRR302 pKa = 11.84GTIQTQGDD310 pKa = 3.67YY311 pKa = 11.39ADD313 pKa = 4.24AVNAYY318 pKa = 10.09SIGGGGGHH326 pKa = 6.87GGSAVTAGGLVGVAIGGSGGGGGDD350 pKa = 3.49SGSVALDD357 pKa = 3.22NFGTIIAAGTNAKK370 pKa = 10.15GMVGSSIGGGGGDD383 pKa = 3.66ANAVTNVTYY392 pKa = 10.88GPGAAWNFSFGGSGGMGGTGGAVVVDD418 pKa = 3.79NHH420 pKa = 6.73RR421 pKa = 11.84GGTIVTGAPTSNAPQDD437 pKa = 4.03PGNVPIPGDD446 pKa = 3.57HH447 pKa = 7.22ASGILAQSLGGGGGHH462 pKa = 7.1GAQTVSVSASAGYY475 pKa = 10.85ASFALGVALGGTGGAGGSGGTVSVTSDD502 pKa = 3.1GSITTHH508 pKa = 6.25ARR510 pKa = 11.84LADD513 pKa = 4.66GILAQSIGGGGGKK526 pKa = 10.28GGTSINVDD534 pKa = 3.08ASVGEE539 pKa = 4.21YY540 pKa = 10.46AGNLSIVLGGNGGNGGNGDD559 pKa = 4.01AVSVDD564 pKa = 3.42LGGAISTYY572 pKa = 10.23SSQSRR577 pKa = 11.84GIFAQSIGGGGGSGGNIINVDD598 pKa = 3.4AAVGSNAANLSVSLGGTGGGGGYY621 pKa = 10.08GSSVSVTTDD630 pKa = 2.7AGSTIATLGHH640 pKa = 6.2YY641 pKa = 10.27SDD643 pKa = 6.17GILAQSIGGGGGGGGSVNSYY663 pKa = 10.69AISAINGSGLAASAGVGVGGLGGSGGAGGDD693 pKa = 3.4VTVSHH698 pKa = 6.83GGAITTAGDD707 pKa = 3.41FATGLFAQSVGGGGGVGGSVFEE729 pKa = 4.37LSIAASLDD737 pKa = 3.37AQNAQGNALGGRR749 pKa = 11.84DD750 pKa = 3.37IAASIAVGGAGGAGNVGGDD769 pKa = 3.56VTVSLQSGSSIVTTGEE785 pKa = 3.71SAAGIIAQSIGGGGGAGGIAHH806 pKa = 6.92SFAVSTPVPTSPQRR820 pKa = 11.84LADD823 pKa = 4.49LLKK826 pKa = 9.88QWRR829 pKa = 11.84AMAEE833 pKa = 4.33KK834 pKa = 9.79IFSSQTSDD842 pKa = 3.33GPAGQYY848 pKa = 10.13QSGLQATISVGGNGGDD864 pKa = 3.34AGIGGNVIVNLASNASIQTSGSSSHH889 pKa = 6.07GVYY892 pKa = 10.53ALSIGGGGGIGGMANSDD909 pKa = 3.44GFAGFGEE916 pKa = 4.48FGLALSLGGNGGNDD930 pKa = 3.34NDD932 pKa = 4.37GGSVDD937 pKa = 4.0VVGSGPDD944 pKa = 3.23GTTAGIVTAGQLSHH958 pKa = 6.99GIAAQSVGGGGGSSASGGTGSTSDD982 pKa = 3.39HH983 pKa = 6.93SIPGLSRR990 pKa = 11.84QTVSMAIGGNAGAQGDD1006 pKa = 4.2GGSVRR1011 pKa = 11.84VIDD1014 pKa = 4.45APNIVTSGGGSDD1026 pKa = 5.14AIFAQSVGGGGGLGGAGSATGLIDD1050 pKa = 3.72ISLGGKK1056 pKa = 9.63GGAGGNGSTVRR1067 pKa = 11.84IKK1069 pKa = 10.94GDD1071 pKa = 3.94SILSTSGSASAGIFAQSVGGGGGAAGISNATLFGLQAGFDD1111 pKa = 3.86LRR1113 pKa = 11.84NGGIAGNGGQGGNVDD1128 pKa = 3.75VTWNGRR1134 pKa = 11.84IQTTGAVSIGIFAQSIGGGGGAVAANDD1161 pKa = 3.53LTIVGGAVPFYY1172 pKa = 9.64SLPGGGVGNGGTVTVGDD1189 pKa = 4.51LANDD1193 pKa = 4.12PLRR1196 pKa = 11.84LSTSGDD1202 pKa = 3.48GAHH1205 pKa = 7.11GIVAQSIGGGGVGLFADD1222 pKa = 4.07QPTEE1226 pKa = 3.87LFDD1229 pKa = 4.89VIWTAGGTPNGMGGSVIVTLNGSVATSGANAYY1261 pKa = 10.27GIVAQSLTNATVLFGTDD1278 pKa = 3.48GIEE1281 pKa = 4.07IVQEE1285 pKa = 4.15PTPSAQGAVQVILQGSVTTQGVGAHH1310 pKa = 7.21GIATYY1315 pKa = 10.52MNTTQAGNPVVQATGTVNVSGAGAWGIYY1343 pKa = 5.93TTNGQDD1349 pKa = 3.39GFGAPSTEE1357 pKa = 4.17AFTTDD1362 pKa = 2.8ISIQAGGTINATGAAAGAINMADD1385 pKa = 3.25WNGEE1389 pKa = 3.65ATADD1393 pKa = 2.84IWGALNAPDD1402 pKa = 5.2AVALQSNAAGLLNVHH1417 pKa = 6.34SGGVVLGDD1425 pKa = 3.47IVATGGDD1432 pKa = 4.31FILLNEE1438 pKa = 4.19GSITGSVSGVSTYY1451 pKa = 11.03AIGGGSHH1458 pKa = 7.2FLRR1461 pKa = 11.84FDD1463 pKa = 3.24PTATLGSDD1471 pKa = 4.18SIAVQQLEE1479 pKa = 4.45VPSNSIHH1486 pKa = 6.58PVLIALPNGNVTATQIIEE1504 pKa = 4.24THH1506 pKa = 6.2GGADD1510 pKa = 4.02LTPVPADD1517 pKa = 3.29QVGSFGLSSGTIATQYY1533 pKa = 11.26DD1534 pKa = 3.9YY1535 pKa = 11.74DD1536 pKa = 4.16FTLSTATIADD1546 pKa = 3.36VSIDD1550 pKa = 3.5FTRR1553 pKa = 11.84GGFSGNTQQLASAANNQLLGWAADD1577 pKa = 3.78APTSPSALYY1586 pKa = 9.61TLLLNASNATTSAEE1600 pKa = 3.74LSQYY1604 pKa = 10.04LQNLDD1609 pKa = 3.19ATASYY1614 pKa = 11.02AAVQQSAMAASAAHH1628 pKa = 5.67TTMQSCGNPVGLYY1641 pKa = 10.55SLIDD1645 pKa = 3.42QSPCNWGKK1653 pKa = 8.58ATYY1656 pKa = 10.51AVTTLRR1662 pKa = 11.84DD1663 pKa = 3.02GDD1665 pKa = 3.86QRR1667 pKa = 11.84DD1668 pKa = 3.76TTTGISIGRR1677 pKa = 11.84QDD1679 pKa = 3.55EE1680 pKa = 4.37ASDD1683 pKa = 3.54NVYY1686 pKa = 10.89VGGSFAYY1693 pKa = 10.07DD1694 pKa = 2.96WTSFDD1699 pKa = 4.02GVGSSSDD1706 pKa = 3.4GNRR1709 pKa = 11.84VSLGAIAKK1717 pKa = 8.61YY1718 pKa = 10.8VEE1720 pKa = 4.27GPVYY1724 pKa = 10.75ASASVVGSYY1733 pKa = 9.56GWADD1737 pKa = 3.4GSRR1740 pKa = 11.84YY1741 pKa = 10.17SSLSWVNGVATADD1754 pKa = 3.54QEE1756 pKa = 4.55TWALTGRR1763 pKa = 11.84LRR1765 pKa = 11.84AGYY1768 pKa = 9.8VFDD1771 pKa = 3.84VGAVEE1776 pKa = 5.71LMPLVDD1782 pKa = 5.79FDD1784 pKa = 3.89VQGIFDD1790 pKa = 3.39QGYY1793 pKa = 7.29TEE1795 pKa = 5.07QGLDD1799 pKa = 3.67DD1800 pKa = 4.98LALQVSNSNNVLFDD1814 pKa = 3.75LRR1816 pKa = 11.84PALRR1820 pKa = 11.84IGGTTKK1826 pKa = 10.57VNDD1829 pKa = 3.99AYY1831 pKa = 9.53ITNHH1835 pKa = 5.29VEE1837 pKa = 3.45IGALFALNEE1846 pKa = 4.1GSVDD1850 pKa = 3.33VSLPNGPNPTASVPLALEE1868 pKa = 4.46RR1869 pKa = 11.84DD1870 pKa = 3.77DD1871 pKa = 4.95VMATIALGSSIDD1883 pKa = 3.16WGAYY1887 pKa = 6.96EE1888 pKa = 6.11LRR1890 pKa = 11.84LQYY1893 pKa = 10.62EE1894 pKa = 4.53GALGDD1899 pKa = 3.94TTVSQAGSVKK1909 pKa = 10.16FAIKK1913 pKa = 10.25FF1914 pKa = 3.5

Molecular weight: 181.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5TS36|A0A2S5TS36_9RHIZ Fatty acid desaturase OS=Kaistia algarum OX=2083279 GN=C3941_22845 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.74LVRR12 pKa = 11.84TRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.65GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.55KK41 pKa = 9.66LTAA44 pKa = 4.17

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6004

0

6004

1794267

20

2242

298.8

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.395 ± 0.051

0.741 ± 0.01

5.635 ± 0.028

5.565 ± 0.031

3.898 ± 0.021

8.618 ± 0.038

1.971 ± 0.015

5.74 ± 0.032

3.409 ± 0.03

10.19 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.013

2.687 ± 0.021

5.137 ± 0.026

3.051 ± 0.021

6.68 ± 0.035

5.652 ± 0.024

5.205 ± 0.022

7.453 ± 0.03

1.323 ± 0.012

2.32 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski