Amino acid dipepetide frequency for Salmonella phage VB_StyS_BS5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.313AlaAla: 9.313 ± 2.606
0.417AlaCys: 0.417 ± 0.179
3.822AlaAsp: 3.822 ± 0.467
6.741AlaGlu: 6.741 ± 0.882
2.71AlaPhe: 2.71 ± 0.487
6.741AlaGly: 6.741 ± 0.743
1.112AlaHis: 1.112 ± 0.335
5.282AlaIle: 5.282 ± 0.586
6.672AlaLys: 6.672 ± 0.898
6.95AlaLeu: 6.95 ± 0.796
3.127AlaMet: 3.127 ± 0.517
3.822AlaAsn: 3.822 ± 0.645
1.876AlaPro: 1.876 ± 0.294
3.614AlaGln: 3.614 ± 0.938
4.726AlaArg: 4.726 ± 0.629
4.587AlaSer: 4.587 ± 0.615
4.934AlaThr: 4.934 ± 1.058
6.116AlaVal: 6.116 ± 0.655
1.251AlaTrp: 1.251 ± 0.254
2.71AlaTyr: 2.71 ± 0.343
0.0AlaXaa: 0.0 ± 0.0
Cys
1.32CysAla: 1.32 ± 0.295
0.417CysCys: 0.417 ± 0.198
0.834CysAsp: 0.834 ± 0.224
1.042CysGlu: 1.042 ± 0.311
0.764CysPhe: 0.764 ± 0.268
1.598CysGly: 1.598 ± 0.469
0.764CysHis: 0.764 ± 0.199
0.973CysIle: 0.973 ± 0.286
1.459CysLys: 1.459 ± 0.359
0.834CysLeu: 0.834 ± 0.252
0.208CysMet: 0.208 ± 0.115
0.973CysAsn: 0.973 ± 0.301
0.556CysPro: 0.556 ± 0.226
0.417CysGln: 0.417 ± 0.196
0.973CysArg: 0.973 ± 0.358
0.834CysSer: 0.834 ± 0.247
0.556CysThr: 0.556 ± 0.221
0.556CysVal: 0.556 ± 0.209
0.278CysTrp: 0.278 ± 0.135
0.625CysTyr: 0.625 ± 0.216
0.0CysXaa: 0.0 ± 0.0
Asp
6.394AspAla: 6.394 ± 0.565
1.32AspCys: 1.32 ± 0.351
5.49AspAsp: 5.49 ± 0.873
3.961AspGlu: 3.961 ± 0.591
3.266AspPhe: 3.266 ± 0.608
6.602AspGly: 6.602 ± 0.842
0.973AspHis: 0.973 ± 0.271
4.726AspIle: 4.726 ± 0.555
3.961AspLys: 3.961 ± 0.738
2.919AspLeu: 2.919 ± 0.527
1.598AspMet: 1.598 ± 0.418
2.363AspAsn: 2.363 ± 0.412
1.39AspPro: 1.39 ± 0.332
1.459AspGln: 1.459 ± 0.419
2.78AspArg: 2.78 ± 0.491
3.405AspSer: 3.405 ± 0.653
2.641AspThr: 2.641 ± 0.494
4.239AspVal: 4.239 ± 0.596
0.764AspTrp: 0.764 ± 0.239
2.78AspTyr: 2.78 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
5.212GluAla: 5.212 ± 0.627
1.251GluCys: 1.251 ± 0.327
3.475GluAsp: 3.475 ± 0.421
4.656GluGlu: 4.656 ± 0.548
2.432GluPhe: 2.432 ± 0.45
2.988GluGly: 2.988 ± 0.426
0.834GluHis: 0.834 ± 0.251
5.004GluIle: 5.004 ± 0.495
4.17GluLys: 4.17 ± 0.641
5.907GluLeu: 5.907 ± 0.604
2.71GluMet: 2.71 ± 0.444
2.432GluAsn: 2.432 ± 0.391
2.293GluPro: 2.293 ± 0.428
3.892GluGln: 3.892 ± 0.554
3.336GluArg: 3.336 ± 0.539
3.822GluSer: 3.822 ± 0.549
2.641GluThr: 2.641 ± 0.382
3.266GluVal: 3.266 ± 0.581
1.112GluTrp: 1.112 ± 0.245
2.641GluTyr: 2.641 ± 0.467
0.0GluXaa: 0.0 ± 0.0
Phe
1.946PheAla: 1.946 ± 0.42
0.973PheCys: 0.973 ± 0.246
3.336PheAsp: 3.336 ± 0.466
2.015PheGlu: 2.015 ± 0.334
0.973PhePhe: 0.973 ± 0.284
2.502PheGly: 2.502 ± 0.306
0.695PheHis: 0.695 ± 0.189
2.919PheIle: 2.919 ± 0.574
1.598PheLys: 1.598 ± 0.255
2.085PheLeu: 2.085 ± 0.412
1.459PheMet: 1.459 ± 0.298
3.266PheAsn: 3.266 ± 0.393
1.459PhePro: 1.459 ± 0.283
1.181PheGln: 1.181 ± 0.293
1.807PheArg: 1.807 ± 0.336
2.571PheSer: 2.571 ± 0.576
1.668PheThr: 1.668 ± 0.348
1.946PheVal: 1.946 ± 0.351
0.695PheTrp: 0.695 ± 0.201
1.112PheTyr: 1.112 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
5.977GlyAla: 5.977 ± 0.642
1.598GlyCys: 1.598 ± 0.432
5.073GlyAsp: 5.073 ± 0.644
4.934GlyGlu: 4.934 ± 0.65
2.71GlyPhe: 2.71 ± 0.455
5.073GlyGly: 5.073 ± 0.777
0.973GlyHis: 0.973 ± 0.291
4.656GlyIle: 4.656 ± 0.518
5.977GlyLys: 5.977 ± 0.611
4.934GlyLeu: 4.934 ± 0.613
2.224GlyMet: 2.224 ± 0.359
3.058GlyAsn: 3.058 ± 0.352
0.903GlyPro: 0.903 ± 0.23
1.946GlyGln: 1.946 ± 0.373
3.683GlyArg: 3.683 ± 0.479
5.49GlySer: 5.49 ± 0.772
3.892GlyThr: 3.892 ± 0.676
5.768GlyVal: 5.768 ± 0.709
1.251GlyTrp: 1.251 ± 0.373
3.753GlyTyr: 3.753 ± 0.577
0.0GlyXaa: 0.0 ± 0.0
His
0.903HisAla: 0.903 ± 0.355
0.347HisCys: 0.347 ± 0.171
1.112HisAsp: 1.112 ± 0.294
1.112HisGlu: 1.112 ± 0.29
0.625HisPhe: 0.625 ± 0.204
2.502HisGly: 2.502 ± 0.688
0.625HisHis: 0.625 ± 0.22
1.042HisIle: 1.042 ± 0.388
1.39HisLys: 1.39 ± 0.353
1.598HisLeu: 1.598 ± 0.331
0.625HisMet: 0.625 ± 0.23
0.834HisAsn: 0.834 ± 0.226
0.764HisPro: 0.764 ± 0.191
0.625HisGln: 0.625 ± 0.177
1.042HisArg: 1.042 ± 0.318
0.764HisSer: 0.764 ± 0.242
0.625HisThr: 0.625 ± 0.248
1.32HisVal: 1.32 ± 0.365
0.208HisTrp: 0.208 ± 0.115
0.903HisTyr: 0.903 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
6.116IleAla: 6.116 ± 0.683
1.39IleCys: 1.39 ± 0.404
5.143IleAsp: 5.143 ± 0.642
4.239IleGlu: 4.239 ± 0.469
2.432IlePhe: 2.432 ± 0.403
5.143IleGly: 5.143 ± 0.677
1.32IleHis: 1.32 ± 0.314
4.795IleIle: 4.795 ± 0.701
4.17IleLys: 4.17 ± 0.53
4.239IleLeu: 4.239 ± 0.665
2.015IleMet: 2.015 ± 0.445
3.544IleAsn: 3.544 ± 0.51
2.641IlePro: 2.641 ± 0.368
1.668IleGln: 1.668 ± 0.389
4.239IleArg: 4.239 ± 0.533
4.448IleSer: 4.448 ± 0.552
4.448IleThr: 4.448 ± 0.667
4.239IleVal: 4.239 ± 0.504
0.556IleTrp: 0.556 ± 0.235
1.737IleTyr: 1.737 ± 0.34
0.0IleXaa: 0.0 ± 0.0
Lys
6.046LysAla: 6.046 ± 0.884
1.112LysCys: 1.112 ± 0.281
3.544LysAsp: 3.544 ± 0.578
3.127LysGlu: 3.127 ± 0.476
2.571LysPhe: 2.571 ± 0.482
3.614LysGly: 3.614 ± 0.587
1.598LysHis: 1.598 ± 0.491
4.448LysIle: 4.448 ± 0.677
4.309LysLys: 4.309 ± 0.672
5.421LysLeu: 5.421 ± 0.859
2.849LysMet: 2.849 ± 0.448
2.432LysAsn: 2.432 ± 0.479
2.154LysPro: 2.154 ± 0.392
2.71LysGln: 2.71 ± 0.492
3.405LysArg: 3.405 ± 0.462
4.378LysSer: 4.378 ± 0.544
3.822LysThr: 3.822 ± 0.572
4.1LysVal: 4.1 ± 0.507
0.973LysTrp: 0.973 ± 0.301
2.502LysTyr: 2.502 ± 0.406
0.0LysXaa: 0.0 ± 0.0
Leu
4.726LeuAla: 4.726 ± 0.704
0.903LeuCys: 0.903 ± 0.25
3.127LeuAsp: 3.127 ± 0.467
4.726LeuGlu: 4.726 ± 0.498
2.363LeuPhe: 2.363 ± 0.384
4.934LeuGly: 4.934 ± 0.59
1.042LeuHis: 1.042 ± 0.273
4.865LeuIle: 4.865 ± 0.585
4.239LeuLys: 4.239 ± 0.681
4.587LeuLeu: 4.587 ± 0.623
2.015LeuMet: 2.015 ± 0.367
3.336LeuAsn: 3.336 ± 0.573
2.849LeuPro: 2.849 ± 0.609
2.641LeuGln: 2.641 ± 0.541
4.795LeuArg: 4.795 ± 0.568
5.143LeuSer: 5.143 ± 0.587
5.004LeuThr: 5.004 ± 0.56
5.351LeuVal: 5.351 ± 0.717
1.529LeuTrp: 1.529 ± 0.313
2.432LeuTyr: 2.432 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
3.475MetAla: 3.475 ± 0.502
0.486MetCys: 0.486 ± 0.194
1.598MetAsp: 1.598 ± 0.338
1.459MetGlu: 1.459 ± 0.261
1.112MetPhe: 1.112 ± 0.267
1.042MetGly: 1.042 ± 0.261
0.903MetHis: 0.903 ± 0.242
1.807MetIle: 1.807 ± 0.427
3.197MetLys: 3.197 ± 0.479
1.807MetLeu: 1.807 ± 0.328
1.32MetMet: 1.32 ± 0.268
1.668MetAsn: 1.668 ± 0.302
1.529MetPro: 1.529 ± 0.395
1.529MetGln: 1.529 ± 0.361
1.946MetArg: 1.946 ± 0.347
2.641MetSer: 2.641 ± 0.436
1.39MetThr: 1.39 ± 0.325
1.112MetVal: 1.112 ± 0.265
0.347MetTrp: 0.347 ± 0.136
0.903MetTyr: 0.903 ± 0.263
0.0MetXaa: 0.0 ± 0.0
Asn
3.683AsnAla: 3.683 ± 0.486
0.417AsnCys: 0.417 ± 0.161
3.336AsnAsp: 3.336 ± 0.549
2.502AsnGlu: 2.502 ± 0.482
1.181AsnPhe: 1.181 ± 0.349
4.517AsnGly: 4.517 ± 0.542
1.39AsnHis: 1.39 ± 0.374
3.127AsnIle: 3.127 ± 0.402
2.919AsnLys: 2.919 ± 0.488
3.266AsnLeu: 3.266 ± 0.424
1.251AsnMet: 1.251 ± 0.338
2.224AsnAsn: 2.224 ± 0.385
1.598AsnPro: 1.598 ± 0.373
1.251AsnGln: 1.251 ± 0.288
2.641AsnArg: 2.641 ± 0.338
2.919AsnSer: 2.919 ± 0.548
2.293AsnThr: 2.293 ± 0.457
2.988AsnVal: 2.988 ± 0.641
0.695AsnTrp: 0.695 ± 0.223
1.668AsnTyr: 1.668 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
2.432ProAla: 2.432 ± 0.447
0.556ProCys: 0.556 ± 0.208
2.849ProAsp: 2.849 ± 0.507
2.849ProGlu: 2.849 ± 0.446
0.973ProPhe: 0.973 ± 0.25
2.015ProGly: 2.015 ± 0.343
0.556ProHis: 0.556 ± 0.185
1.876ProIle: 1.876 ± 0.343
1.39ProLys: 1.39 ± 0.336
1.807ProLeu: 1.807 ± 0.411
0.764ProMet: 0.764 ± 0.216
1.181ProAsn: 1.181 ± 0.318
0.903ProPro: 0.903 ± 0.291
1.32ProGln: 1.32 ± 0.382
1.251ProArg: 1.251 ± 0.311
2.015ProSer: 2.015 ± 0.353
1.668ProThr: 1.668 ± 0.361
3.127ProVal: 3.127 ± 0.507
0.486ProTrp: 0.486 ± 0.226
1.459ProTyr: 1.459 ± 0.356
0.0ProXaa: 0.0 ± 0.0
Gln
3.127GlnAla: 3.127 ± 0.869
0.347GlnCys: 0.347 ± 0.168
1.668GlnAsp: 1.668 ± 0.338
2.571GlnGlu: 2.571 ± 0.344
1.598GlnPhe: 1.598 ± 0.344
1.668GlnGly: 1.668 ± 0.443
1.112GlnHis: 1.112 ± 0.286
2.502GlnIle: 2.502 ± 0.44
2.085GlnLys: 2.085 ± 0.435
3.753GlnLeu: 3.753 ± 0.687
1.598GlnMet: 1.598 ± 0.303
1.32GlnAsn: 1.32 ± 0.27
1.39GlnPro: 1.39 ± 0.33
2.502GlnGln: 2.502 ± 0.74
1.737GlnArg: 1.737 ± 0.378
2.849GlnSer: 2.849 ± 0.516
2.015GlnThr: 2.015 ± 0.482
2.571GlnVal: 2.571 ± 0.402
0.556GlnTrp: 0.556 ± 0.214
1.181GlnTyr: 1.181 ± 0.244
0.0GlnXaa: 0.0 ± 0.0
Arg
4.795ArgAla: 4.795 ± 0.583
1.32ArgCys: 1.32 ± 0.413
2.849ArgAsp: 2.849 ± 0.43
3.683ArgGlu: 3.683 ± 0.496
1.876ArgPhe: 1.876 ± 0.369
3.544ArgGly: 3.544 ± 0.524
0.625ArgHis: 0.625 ± 0.201
3.544ArgIle: 3.544 ± 0.528
4.448ArgLys: 4.448 ± 0.677
4.378ArgLeu: 4.378 ± 0.589
1.251ArgMet: 1.251 ± 0.315
3.336ArgAsn: 3.336 ± 0.413
1.112ArgPro: 1.112 ± 0.237
1.737ArgGln: 1.737 ± 0.436
3.058ArgArg: 3.058 ± 0.509
2.849ArgSer: 2.849 ± 0.412
2.363ArgThr: 2.363 ± 0.431
3.266ArgVal: 3.266 ± 0.54
0.903ArgTrp: 0.903 ± 0.247
2.502ArgTyr: 2.502 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
5.351SerAla: 5.351 ± 0.675
1.112SerCys: 1.112 ± 0.277
4.378SerAsp: 4.378 ± 0.638
3.614SerGlu: 3.614 ± 0.477
2.641SerPhe: 2.641 ± 0.456
6.602SerGly: 6.602 ± 0.616
0.973SerHis: 0.973 ± 0.249
3.822SerIle: 3.822 ± 0.571
3.822SerLys: 3.822 ± 0.546
5.56SerLeu: 5.56 ± 0.722
1.807SerMet: 1.807 ± 0.425
2.154SerAsn: 2.154 ± 0.341
2.224SerPro: 2.224 ± 0.417
2.71SerGln: 2.71 ± 0.444
3.405SerArg: 3.405 ± 0.58
3.405SerSer: 3.405 ± 0.633
4.17SerThr: 4.17 ± 0.725
3.336SerVal: 3.336 ± 0.565
1.112SerTrp: 1.112 ± 0.272
2.293SerTyr: 2.293 ± 0.418
0.0SerXaa: 0.0 ± 0.0
Thr
5.629ThrAla: 5.629 ± 1.088
0.764ThrCys: 0.764 ± 0.272
2.919ThrAsp: 2.919 ± 0.461
4.17ThrGlu: 4.17 ± 0.532
1.668ThrPhe: 1.668 ± 0.404
5.282ThrGly: 5.282 ± 0.567
0.903ThrHis: 0.903 ± 0.257
3.405ThrIle: 3.405 ± 0.433
2.849ThrLys: 2.849 ± 0.444
3.197ThrLeu: 3.197 ± 0.616
1.251ThrMet: 1.251 ± 0.228
2.293ThrAsn: 2.293 ± 0.472
2.78ThrPro: 2.78 ± 0.46
2.015ThrGln: 2.015 ± 0.379
2.919ThrArg: 2.919 ± 0.514
3.266ThrSer: 3.266 ± 0.562
3.892ThrThr: 3.892 ± 0.638
2.919ThrVal: 2.919 ± 0.482
0.625ThrTrp: 0.625 ± 0.23
1.876ThrTyr: 1.876 ± 0.41
0.0ThrXaa: 0.0 ± 0.0
Val
6.185ValAla: 6.185 ± 0.801
0.347ValCys: 0.347 ± 0.183
4.448ValAsp: 4.448 ± 0.548
3.961ValGlu: 3.961 ± 0.494
2.363ValPhe: 2.363 ± 0.502
3.822ValGly: 3.822 ± 0.561
1.251ValHis: 1.251 ± 0.285
6.255ValIle: 6.255 ± 0.787
3.753ValLys: 3.753 ± 0.598
3.753ValLeu: 3.753 ± 0.512
2.224ValMet: 2.224 ± 0.319
3.544ValAsn: 3.544 ± 0.454
1.529ValPro: 1.529 ± 0.325
2.363ValGln: 2.363 ± 0.359
2.849ValArg: 2.849 ± 0.408
5.143ValSer: 5.143 ± 0.544
3.475ValThr: 3.475 ± 0.754
4.726ValVal: 4.726 ± 0.757
1.042ValTrp: 1.042 ± 0.228
2.363ValTyr: 2.363 ± 0.332
0.0ValXaa: 0.0 ± 0.0
Trp
1.042TrpAla: 1.042 ± 0.293
0.208TrpCys: 0.208 ± 0.127
0.625TrpAsp: 0.625 ± 0.243
0.834TrpGlu: 0.834 ± 0.282
0.625TrpPhe: 0.625 ± 0.217
0.695TrpGly: 0.695 ± 0.231
0.417TrpHis: 0.417 ± 0.177
1.181TrpIle: 1.181 ± 0.352
0.973TrpLys: 0.973 ± 0.313
1.39TrpLeu: 1.39 ± 0.337
0.208TrpMet: 0.208 ± 0.129
0.695TrpAsn: 0.695 ± 0.237
0.486TrpPro: 0.486 ± 0.171
0.834TrpGln: 0.834 ± 0.243
1.32TrpArg: 1.32 ± 0.328
0.695TrpSer: 0.695 ± 0.211
1.112TrpThr: 1.112 ± 0.267
1.251TrpVal: 1.251 ± 0.283
0.278TrpTrp: 0.278 ± 0.135
0.417TrpTyr: 0.417 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.849TyrAla: 2.849 ± 0.404
0.695TyrCys: 0.695 ± 0.186
3.405TyrAsp: 3.405 ± 0.517
1.876TyrGlu: 1.876 ± 0.332
1.32TyrPhe: 1.32 ± 0.296
2.988TyrGly: 2.988 ± 0.52
0.903TyrHis: 0.903 ± 0.272
2.293TyrIle: 2.293 ± 0.383
1.737TyrLys: 1.737 ± 0.406
2.224TyrLeu: 2.224 ± 0.453
0.695TyrMet: 0.695 ± 0.197
1.39TyrAsn: 1.39 ± 0.358
1.042TyrPro: 1.042 ± 0.28
1.737TyrGln: 1.737 ± 0.338
1.598TyrArg: 1.598 ± 0.348
3.266TyrSer: 3.266 ± 0.507
2.015TyrThr: 2.015 ± 0.442
3.197TyrVal: 3.197 ± 0.43
0.625TyrTrp: 0.625 ± 0.182
1.181TyrTyr: 1.181 ± 0.281
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (14390 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski