Acinonyx jubatus (Cheetah)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; Feliformia;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J2AB61|A0A6J2AB61_ACIJB cytoskeleton-associated protein 4 OS=Acinonyx jubatus OX=32536 GN=CKAP4 PE=4 SV=1
MM1 pKa = 8.11PEE3 pKa = 4.97PILDD7 pKa = 3.99FNCNYY12 pKa = 8.32VTADD16 pKa = 3.28PDD18 pKa = 3.73YY19 pKa = 10.26QEE21 pKa = 5.45KK22 pKa = 10.21YY23 pKa = 9.86QEE25 pKa = 4.02LLEE28 pKa = 5.41RR29 pKa = 11.84EE30 pKa = 4.57DD31 pKa = 4.33FFPDD35 pKa = 3.59YY36 pKa = 10.5EE37 pKa = 4.55EE38 pKa = 5.11NGIDD42 pKa = 3.69QSAAGDD48 pKa = 4.01PYY50 pKa = 11.36LDD52 pKa = 5.4DD53 pKa = 6.11GDD55 pKa = 5.97DD56 pKa = 3.75EE57 pKa = 4.73MDD59 pKa = 4.4PEE61 pKa = 4.49TEE63 pKa = 3.56EE64 pKa = 4.95AYY66 pKa = 11.29NNLVWTQSISEE77 pKa = 4.03SDD79 pKa = 3.12KK80 pKa = 11.7AKK82 pKa = 9.97FQHH85 pKa = 6.37ISFIKK90 pKa = 10.6QFGLWW95 pKa = 3.24

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J1Z4W6|A0A6J1Z4W6_ACIJB LOW QUALITY PROTEIN: ADAMTS-like protein 4 OS=Acinonyx jubatus OX=32536 GN=ADAMTSL4 PE=4 SV=1
RR1 pKa = 7.24SRR3 pKa = 11.84RR4 pKa = 11.84HH5 pKa = 4.48CRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84LSRR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84RR16 pKa = 11.84LTRR19 pKa = 11.84SRR21 pKa = 11.84RR22 pKa = 11.84PRR24 pKa = 11.84RR25 pKa = 11.84LSRR28 pKa = 11.84SHH30 pKa = 6.38RR31 pKa = 11.84GSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84HH37 pKa = 4.99SRR39 pKa = 11.84HH40 pKa = 5.95LSRR43 pKa = 11.84THH45 pKa = 5.05SRR47 pKa = 11.84RR48 pKa = 11.84SRR50 pKa = 11.84RR51 pKa = 11.84SRR53 pKa = 11.84NRR55 pKa = 11.84SRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84HH60 pKa = 4.56GSRR63 pKa = 11.84LIRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84LSRR71 pKa = 11.84RR72 pKa = 11.84LSRR75 pKa = 11.84LRR77 pKa = 11.84RR78 pKa = 11.84LSHH81 pKa = 5.63GCRR84 pKa = 11.84RR85 pKa = 11.84CRR87 pKa = 11.84HH88 pKa = 6.19CSHH91 pKa = 5.84SHH93 pKa = 5.54RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84HH97 pKa = 5.88RR98 pKa = 11.84NRR100 pKa = 11.84LSRR103 pKa = 11.84RR104 pKa = 11.84LSRR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84LKK111 pKa = 10.32RR112 pKa = 11.84NRR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84LSRR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84CRR126 pKa = 11.84SHH128 pKa = 6.82HH129 pKa = 5.6SRR131 pKa = 11.84SRR133 pKa = 11.84LTRR136 pKa = 11.84SRR138 pKa = 11.84SRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84PPPRR149 pKa = 11.84APGRR153 pKa = 11.84VAEE156 pKa = 5.11AEE158 pKa = 4.18PEE160 pKa = 4.2DD161 pKa = 4.36PSNALWRR168 pKa = 11.84KK169 pKa = 7.7PRR171 pKa = 11.84CSLTPGLLPAAPGLAPRR188 pKa = 11.84PPPLPSRR195 pKa = 11.84YY196 pKa = 9.04AAQRR200 pKa = 11.84TAPHH204 pKa = 6.84LAAPHH209 pKa = 5.77HH210 pKa = 5.83TAPHH214 pKa = 5.11YY215 pKa = 10.11TMMGCSVGRR224 pKa = 11.84GWKK227 pKa = 9.68

Molecular weight: 27.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19411

25905

45316

33444246

31

34873

738.0

82.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.04 ± 0.012

2.078 ± 0.01

4.887 ± 0.008

7.348 ± 0.017

3.417 ± 0.008

6.509 ± 0.016

2.584 ± 0.006

4.224 ± 0.011

5.782 ± 0.015

9.795 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.005

3.553 ± 0.009

6.479 ± 0.016

4.926 ± 0.011

5.808 ± 0.011

8.546 ± 0.013

5.321 ± 0.009

5.941 ± 0.011

1.139 ± 0.004

2.533 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski