Amino acid dipepetide frequency for Escherichia phage vB_EcoS_FP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.557AlaAla: 8.557 ± 1.042
0.535AlaCys: 0.535 ± 0.195
4.278AlaAsp: 4.278 ± 0.507
5.348AlaGlu: 5.348 ± 0.612
3.285AlaPhe: 3.285 ± 0.509
5.959AlaGly: 5.959 ± 0.591
0.84AlaHis: 0.84 ± 0.295
7.182AlaIle: 7.182 ± 0.851
6.876AlaLys: 6.876 ± 1.176
7.334AlaLeu: 7.334 ± 1.019
2.063AlaMet: 2.063 ± 0.466
3.973AlaAsn: 3.973 ± 0.627
2.063AlaPro: 2.063 ± 0.399
3.438AlaGln: 3.438 ± 0.678
4.431AlaArg: 4.431 ± 0.637
4.89AlaSer: 4.89 ± 0.576
4.049AlaThr: 4.049 ± 0.583
4.813AlaVal: 4.813 ± 0.556
0.764AlaTrp: 0.764 ± 0.249
2.368AlaTyr: 2.368 ± 0.406
0.0AlaXaa: 0.0 ± 0.0
Cys
0.993CysAla: 0.993 ± 0.341
0.229CysCys: 0.229 ± 0.127
0.917CysAsp: 0.917 ± 0.314
0.764CysGlu: 0.764 ± 0.256
0.229CysPhe: 0.229 ± 0.168
1.07CysGly: 1.07 ± 0.339
0.229CysHis: 0.229 ± 0.136
0.535CysIle: 0.535 ± 0.238
0.917CysLys: 0.917 ± 0.287
1.07CysLeu: 1.07 ± 0.259
0.153CysMet: 0.153 ± 0.091
0.611CysAsn: 0.611 ± 0.273
0.306CysPro: 0.306 ± 0.159
0.229CysGln: 0.229 ± 0.117
0.688CysArg: 0.688 ± 0.278
1.375CysSer: 1.375 ± 0.312
0.84CysThr: 0.84 ± 0.21
0.688CysVal: 0.688 ± 0.239
0.229CysTrp: 0.229 ± 0.132
0.306CysTyr: 0.306 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
4.813AspAla: 4.813 ± 0.697
0.688AspCys: 0.688 ± 0.211
3.591AspAsp: 3.591 ± 0.737
4.813AspGlu: 4.813 ± 0.46
2.598AspPhe: 2.598 ± 0.476
7.258AspGly: 7.258 ± 0.673
1.07AspHis: 1.07 ± 0.378
3.896AspIle: 3.896 ± 0.521
4.049AspLys: 4.049 ± 0.546
4.202AspLeu: 4.202 ± 0.519
1.375AspMet: 1.375 ± 0.336
3.132AspAsn: 3.132 ± 0.512
1.452AspPro: 1.452 ± 0.338
1.146AspGln: 1.146 ± 0.273
1.986AspArg: 1.986 ± 0.403
2.98AspSer: 2.98 ± 0.471
3.362AspThr: 3.362 ± 0.492
5.195AspVal: 5.195 ± 0.747
1.07AspTrp: 1.07 ± 0.258
3.591AspTyr: 3.591 ± 0.576
0.0AspXaa: 0.0 ± 0.0
Glu
5.424GluAla: 5.424 ± 0.586
0.993GluCys: 0.993 ± 0.339
3.209GluAsp: 3.209 ± 0.643
4.202GluGlu: 4.202 ± 0.775
3.285GluPhe: 3.285 ± 0.533
3.362GluGly: 3.362 ± 0.571
0.611GluHis: 0.611 ± 0.235
5.654GluIle: 5.654 ± 0.497
3.744GluLys: 3.744 ± 0.726
5.577GluLeu: 5.577 ± 0.581
2.521GluMet: 2.521 ± 0.492
3.82GluAsn: 3.82 ± 0.472
1.91GluPro: 1.91 ± 0.373
2.903GluGln: 2.903 ± 0.683
2.827GluArg: 2.827 ± 0.482
4.508GluSer: 4.508 ± 0.65
2.98GluThr: 2.98 ± 0.423
5.042GluVal: 5.042 ± 0.736
1.07GluTrp: 1.07 ± 0.368
2.75GluTyr: 2.75 ± 0.391
0.0GluXaa: 0.0 ± 0.0
Phe
2.292PheAla: 2.292 ± 0.391
0.688PheCys: 0.688 ± 0.235
3.514PheAsp: 3.514 ± 0.417
2.827PheGlu: 2.827 ± 0.538
0.917PhePhe: 0.917 ± 0.309
3.438PheGly: 3.438 ± 0.599
0.764PheHis: 0.764 ± 0.292
2.445PheIle: 2.445 ± 0.442
2.674PheLys: 2.674 ± 0.512
2.063PheLeu: 2.063 ± 0.432
0.84PheMet: 0.84 ± 0.245
2.598PheAsn: 2.598 ± 0.402
1.299PhePro: 1.299 ± 0.3
1.834PheGln: 1.834 ± 0.365
1.757PheArg: 1.757 ± 0.491
2.445PheSer: 2.445 ± 0.437
2.292PheThr: 2.292 ± 0.318
2.445PheVal: 2.445 ± 0.397
0.382PheTrp: 0.382 ± 0.173
1.07PheTyr: 1.07 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
4.202GlyAla: 4.202 ± 0.658
1.222GlyCys: 1.222 ± 0.34
4.89GlyAsp: 4.89 ± 0.54
5.119GlyGlu: 5.119 ± 0.661
2.598GlyPhe: 2.598 ± 0.579
6.265GlyGly: 6.265 ± 1.333
0.688GlyHis: 0.688 ± 0.28
5.73GlyIle: 5.73 ± 0.568
6.265GlyLys: 6.265 ± 0.8
6.418GlyLeu: 6.418 ± 0.623
2.216GlyMet: 2.216 ± 0.515
3.744GlyAsn: 3.744 ± 0.543
0.611GlyPro: 0.611 ± 0.21
2.216GlyGln: 2.216 ± 0.395
2.903GlyArg: 2.903 ± 0.47
5.883GlySer: 5.883 ± 0.844
4.049GlyThr: 4.049 ± 0.617
6.57GlyVal: 6.57 ± 0.758
1.222GlyTrp: 1.222 ± 0.296
3.667GlyTyr: 3.667 ± 0.522
0.0GlyXaa: 0.0 ± 0.0
His
1.452HisAla: 1.452 ± 0.398
0.306HisCys: 0.306 ± 0.155
1.07HisAsp: 1.07 ± 0.301
0.688HisGlu: 0.688 ± 0.207
0.993HisPhe: 0.993 ± 0.247
1.146HisGly: 1.146 ± 0.318
0.382HisHis: 0.382 ± 0.178
1.299HisIle: 1.299 ± 0.368
1.07HisLys: 1.07 ± 0.35
1.375HisLeu: 1.375 ± 0.368
0.306HisMet: 0.306 ± 0.212
0.458HisAsn: 0.458 ± 0.237
0.229HisPro: 0.229 ± 0.136
0.306HisGln: 0.306 ± 0.142
0.917HisArg: 0.917 ± 0.232
0.611HisSer: 0.611 ± 0.253
0.764HisThr: 0.764 ± 0.235
1.07HisVal: 1.07 ± 0.305
0.153HisTrp: 0.153 ± 0.116
0.306HisTyr: 0.306 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
6.494IleAla: 6.494 ± 0.913
0.535IleCys: 0.535 ± 0.191
5.73IleAsp: 5.73 ± 0.571
3.744IleGlu: 3.744 ± 0.406
1.91IlePhe: 1.91 ± 0.26
4.355IleGly: 4.355 ± 0.589
1.452IleHis: 1.452 ± 0.35
3.973IleIle: 3.973 ± 0.711
5.424IleLys: 5.424 ± 0.707
3.973IleLeu: 3.973 ± 0.601
1.604IleMet: 1.604 ± 0.377
4.126IleAsn: 4.126 ± 0.706
2.445IlePro: 2.445 ± 0.483
2.521IleGln: 2.521 ± 0.449
3.896IleArg: 3.896 ± 0.485
5.195IleSer: 5.195 ± 0.569
5.195IleThr: 5.195 ± 0.529
3.362IleVal: 3.362 ± 0.531
0.917IleTrp: 0.917 ± 0.224
3.362IleTyr: 3.362 ± 0.667
0.0IleXaa: 0.0 ± 0.0
Lys
6.57LysAla: 6.57 ± 0.928
0.458LysCys: 0.458 ± 0.21
5.195LysAsp: 5.195 ± 0.679
4.966LysGlu: 4.966 ± 0.654
2.674LysPhe: 2.674 ± 0.397
3.285LysGly: 3.285 ± 0.471
1.222LysHis: 1.222 ± 0.309
4.126LysIle: 4.126 ± 0.609
4.126LysLys: 4.126 ± 0.703
5.348LysLeu: 5.348 ± 0.667
2.521LysMet: 2.521 ± 0.452
3.285LysAsn: 3.285 ± 0.5
1.757LysPro: 1.757 ± 0.424
2.445LysGln: 2.445 ± 0.415
2.98LysArg: 2.98 ± 0.581
4.126LysSer: 4.126 ± 0.539
3.285LysThr: 3.285 ± 0.438
4.278LysVal: 4.278 ± 0.563
0.84LysTrp: 0.84 ± 0.235
3.438LysTyr: 3.438 ± 0.614
0.0LysXaa: 0.0 ± 0.0
Leu
6.036LeuAla: 6.036 ± 0.83
0.917LeuCys: 0.917 ± 0.257
4.508LeuAsp: 4.508 ± 0.604
4.049LeuGlu: 4.049 ± 0.577
2.063LeuPhe: 2.063 ± 0.337
5.424LeuGly: 5.424 ± 0.697
1.07LeuHis: 1.07 ± 0.287
4.89LeuIle: 4.89 ± 0.623
3.82LeuLys: 3.82 ± 0.519
4.049LeuLeu: 4.049 ± 0.423
1.528LeuMet: 1.528 ± 0.396
3.209LeuAsn: 3.209 ± 0.45
3.209LeuPro: 3.209 ± 0.516
2.445LeuGln: 2.445 ± 0.743
3.514LeuArg: 3.514 ± 0.599
6.647LeuSer: 6.647 ± 0.864
4.737LeuThr: 4.737 ± 0.7
5.501LeuVal: 5.501 ± 0.612
0.306LeuTrp: 0.306 ± 0.175
1.681LeuTyr: 1.681 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
3.285MetAla: 3.285 ± 0.581
0.229MetCys: 0.229 ± 0.118
0.993MetAsp: 0.993 ± 0.317
0.917MetGlu: 0.917 ± 0.258
1.07MetPhe: 1.07 ± 0.295
0.993MetGly: 0.993 ± 0.276
0.535MetHis: 0.535 ± 0.211
2.139MetIle: 2.139 ± 0.418
1.91MetLys: 1.91 ± 0.381
1.757MetLeu: 1.757 ± 0.412
0.611MetMet: 0.611 ± 0.266
1.604MetAsn: 1.604 ± 0.382
0.611MetPro: 0.611 ± 0.238
0.917MetGln: 0.917 ± 0.263
1.375MetArg: 1.375 ± 0.286
1.604MetSer: 1.604 ± 0.315
2.445MetThr: 2.445 ± 0.382
1.528MetVal: 1.528 ± 0.324
0.458MetTrp: 0.458 ± 0.207
0.458MetTyr: 0.458 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
4.049AsnAla: 4.049 ± 0.582
0.382AsnCys: 0.382 ± 0.181
2.368AsnAsp: 2.368 ± 0.327
4.66AsnGlu: 4.66 ± 0.601
1.681AsnPhe: 1.681 ± 0.436
5.806AsnGly: 5.806 ± 0.982
0.764AsnHis: 0.764 ± 0.227
2.521AsnIle: 2.521 ± 0.328
3.056AsnLys: 3.056 ± 0.43
3.132AsnLeu: 3.132 ± 0.608
1.299AsnMet: 1.299 ± 0.246
2.75AsnAsn: 2.75 ± 0.367
2.216AsnPro: 2.216 ± 0.311
2.445AsnGln: 2.445 ± 0.47
2.521AsnArg: 2.521 ± 0.369
3.667AsnSer: 3.667 ± 0.583
2.674AsnThr: 2.674 ± 0.449
3.438AsnVal: 3.438 ± 0.465
0.917AsnTrp: 0.917 ± 0.214
1.986AsnTyr: 1.986 ± 0.359
0.0AsnXaa: 0.0 ± 0.0
Pro
3.056ProAla: 3.056 ± 0.498
0.306ProCys: 0.306 ± 0.189
2.063ProAsp: 2.063 ± 0.481
2.598ProGlu: 2.598 ± 0.383
1.528ProPhe: 1.528 ± 0.287
1.986ProGly: 1.986 ± 0.354
0.458ProHis: 0.458 ± 0.167
2.674ProIle: 2.674 ± 0.38
1.375ProLys: 1.375 ± 0.356
1.91ProLeu: 1.91 ± 0.356
0.688ProMet: 0.688 ± 0.225
1.222ProAsn: 1.222 ± 0.28
0.611ProPro: 0.611 ± 0.206
1.222ProGln: 1.222 ± 0.378
1.299ProArg: 1.299 ± 0.302
1.222ProSer: 1.222 ± 0.348
1.528ProThr: 1.528 ± 0.366
2.521ProVal: 2.521 ± 0.529
0.458ProTrp: 0.458 ± 0.219
1.604ProTyr: 1.604 ± 0.442
0.0ProXaa: 0.0 ± 0.0
Gln
3.744GlnAla: 3.744 ± 0.919
0.458GlnCys: 0.458 ± 0.257
1.681GlnAsp: 1.681 ± 0.4
2.98GlnGlu: 2.98 ± 0.612
1.452GlnPhe: 1.452 ± 0.319
2.521GlnGly: 2.521 ± 0.454
0.458GlnHis: 0.458 ± 0.217
3.362GlnIle: 3.362 ± 0.609
1.757GlnLys: 1.757 ± 0.343
2.292GlnLeu: 2.292 ± 0.545
0.917GlnMet: 0.917 ± 0.242
1.299GlnAsn: 1.299 ± 0.325
1.222GlnPro: 1.222 ± 0.383
2.063GlnGln: 2.063 ± 0.549
1.834GlnArg: 1.834 ± 0.418
3.285GlnSer: 3.285 ± 0.524
1.222GlnThr: 1.222 ± 0.291
2.292GlnVal: 2.292 ± 0.36
0.382GlnTrp: 0.382 ± 0.186
1.375GlnTyr: 1.375 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
4.202ArgAla: 4.202 ± 0.685
0.764ArgCys: 0.764 ± 0.321
2.139ArgAsp: 2.139 ± 0.401
3.056ArgGlu: 3.056 ± 0.472
2.368ArgPhe: 2.368 ± 0.384
2.903ArgGly: 2.903 ± 0.469
0.535ArgHis: 0.535 ± 0.223
3.667ArgIle: 3.667 ± 0.539
4.355ArgLys: 4.355 ± 0.593
4.049ArgLeu: 4.049 ± 0.488
0.993ArgMet: 0.993 ± 0.393
1.91ArgAsn: 1.91 ± 0.403
1.757ArgPro: 1.757 ± 0.396
1.528ArgGln: 1.528 ± 0.347
2.368ArgArg: 2.368 ± 0.515
3.209ArgSer: 3.209 ± 0.541
1.986ArgThr: 1.986 ± 0.441
3.514ArgVal: 3.514 ± 0.54
0.458ArgTrp: 0.458 ± 0.142
1.681ArgTyr: 1.681 ± 0.26
0.0ArgXaa: 0.0 ± 0.0
Ser
4.584SerAla: 4.584 ± 0.66
0.535SerCys: 0.535 ± 0.211
4.966SerAsp: 4.966 ± 0.555
4.355SerGlu: 4.355 ± 0.602
2.521SerPhe: 2.521 ± 0.431
7.411SerGly: 7.411 ± 0.855
1.299SerHis: 1.299 ± 0.388
4.508SerIle: 4.508 ± 0.607
3.667SerLys: 3.667 ± 0.513
4.89SerLeu: 4.89 ± 0.645
1.528SerMet: 1.528 ± 0.375
4.278SerAsn: 4.278 ± 0.687
2.292SerPro: 2.292 ± 0.355
2.216SerGln: 2.216 ± 0.424
3.209SerArg: 3.209 ± 0.43
5.424SerSer: 5.424 ± 1.033
4.126SerThr: 4.126 ± 0.633
5.195SerVal: 5.195 ± 0.601
0.611SerTrp: 0.611 ± 0.232
2.827SerTyr: 2.827 ± 0.652
0.0SerXaa: 0.0 ± 0.0
Thr
4.89ThrAla: 4.89 ± 0.718
0.611ThrCys: 0.611 ± 0.252
3.056ThrAsp: 3.056 ± 0.43
2.674ThrGlu: 2.674 ± 0.426
2.674ThrPhe: 2.674 ± 0.461
5.806ThrGly: 5.806 ± 0.764
0.535ThrHis: 0.535 ± 0.199
4.355ThrIle: 4.355 ± 0.501
2.75ThrLys: 2.75 ± 0.492
2.827ThrLeu: 2.827 ± 0.397
0.917ThrMet: 0.917 ± 0.278
3.209ThrAsn: 3.209 ± 0.49
2.598ThrPro: 2.598 ± 0.394
2.216ThrGln: 2.216 ± 0.414
2.292ThrArg: 2.292 ± 0.344
4.126ThrSer: 4.126 ± 0.541
3.132ThrThr: 3.132 ± 0.627
4.126ThrVal: 4.126 ± 0.423
0.382ThrTrp: 0.382 ± 0.182
2.216ThrTyr: 2.216 ± 0.513
0.0ThrXaa: 0.0 ± 0.0
Val
5.348ValAla: 5.348 ± 0.662
1.452ValCys: 1.452 ± 0.342
4.584ValAsp: 4.584 ± 0.466
5.501ValGlu: 5.501 ± 0.88
2.75ValPhe: 2.75 ± 0.352
4.126ValGly: 4.126 ± 0.618
1.07ValHis: 1.07 ± 0.265
3.896ValIle: 3.896 ± 0.454
5.501ValLys: 5.501 ± 0.527
3.82ValLeu: 3.82 ± 0.556
1.986ValMet: 1.986 ± 0.402
4.508ValAsn: 4.508 ± 0.48
2.063ValPro: 2.063 ± 0.423
2.292ValGln: 2.292 ± 0.497
4.202ValArg: 4.202 ± 0.552
4.966ValSer: 4.966 ± 0.621
3.896ValThr: 3.896 ± 0.62
5.348ValVal: 5.348 ± 0.735
0.611ValTrp: 0.611 ± 0.251
2.521ValTyr: 2.521 ± 0.528
0.0ValXaa: 0.0 ± 0.0
Trp
0.458TrpAla: 0.458 ± 0.179
0.458TrpCys: 0.458 ± 0.254
0.993TrpAsp: 0.993 ± 0.252
0.688TrpGlu: 0.688 ± 0.212
0.688TrpPhe: 0.688 ± 0.217
0.917TrpGly: 0.917 ± 0.268
0.229TrpHis: 0.229 ± 0.115
0.84TrpIle: 0.84 ± 0.214
1.146TrpLys: 1.146 ± 0.279
0.993TrpLeu: 0.993 ± 0.274
0.153TrpMet: 0.153 ± 0.111
0.382TrpAsn: 0.382 ± 0.153
0.306TrpPro: 0.306 ± 0.12
0.458TrpGln: 0.458 ± 0.152
0.84TrpArg: 0.84 ± 0.259
0.764TrpSer: 0.764 ± 0.306
0.458TrpThr: 0.458 ± 0.182
0.764TrpVal: 0.764 ± 0.217
0.076TrpTrp: 0.076 ± 0.086
0.229TrpTyr: 0.229 ± 0.12
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.598TyrAla: 2.598 ± 0.456
0.611TyrCys: 0.611 ± 0.193
2.521TyrAsp: 2.521 ± 0.415
2.445TyrGlu: 2.445 ± 0.516
1.299TyrPhe: 1.299 ± 0.321
2.445TyrGly: 2.445 ± 0.375
0.688TyrHis: 0.688 ± 0.216
2.521TyrIle: 2.521 ± 0.449
2.445TyrLys: 2.445 ± 0.467
2.521TyrLeu: 2.521 ± 0.421
1.146TyrMet: 1.146 ± 0.261
2.216TyrAsn: 2.216 ± 0.365
1.375TyrPro: 1.375 ± 0.358
1.757TyrGln: 1.757 ± 0.327
1.681TyrArg: 1.681 ± 0.371
3.514TyrSer: 3.514 ± 0.788
2.368TyrThr: 2.368 ± 0.328
2.75TyrVal: 2.75 ± 0.393
0.535TyrTrp: 0.535 ± 0.214
1.528TyrTyr: 1.528 ± 0.398
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13090 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski