Candidatus Tachikawaea gelatinosa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Tachikawaea

Average proteome isoelectric point is 8.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 613 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090AK87|A0A090AK87_9ENTR Alanine--tRNA ligase OS=Candidatus Tachikawaea gelatinosa OX=1410383 GN=alaS PE=3 SV=1
MM1 pKa = 7.65SDD3 pKa = 2.68IEE5 pKa = 4.12QRR7 pKa = 11.84VKK9 pKa = 10.82KK10 pKa = 10.48IIAEE14 pKa = 3.85QLGVKK19 pKa = 9.71EE20 pKa = 4.22EE21 pKa = 4.25EE22 pKa = 4.51VTNSASFVEE31 pKa = 4.94DD32 pKa = 4.2LGADD36 pKa = 3.39SLDD39 pKa = 3.64TVEE42 pKa = 5.9LVMALEE48 pKa = 4.53EE49 pKa = 4.22EE50 pKa = 4.81FNTEE54 pKa = 3.81IPDD57 pKa = 3.55EE58 pKa = 4.23KK59 pKa = 10.91AEE61 pKa = 4.99KK62 pKa = 9.05ITTVQAAIDD71 pKa = 4.22YY72 pKa = 9.8INSHH76 pKa = 7.19KK77 pKa = 10.6IQQ79 pKa = 3.48

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090BWH7|A0A090BWH7_9ENTR 30S ribosomal protein S21 OS=Candidatus Tachikawaea gelatinosa OX=1410383 GN=rpsU PE=3 SV=1
MM1 pKa = 7.25ATINQLVRR9 pKa = 11.84KK10 pKa = 8.78PRR12 pKa = 11.84IKK14 pKa = 10.41KK15 pKa = 9.16VIKK18 pKa = 10.56SNVPALEE25 pKa = 4.11SCPQKK30 pKa = 10.63RR31 pKa = 11.84GVCTRR36 pKa = 11.84VYY38 pKa = 7.93TTTPKK43 pKa = 10.54KK44 pKa = 10.26PNSALRR50 pKa = 11.84KK51 pKa = 7.38VCRR54 pKa = 11.84VRR56 pKa = 11.84LTNGYY61 pKa = 9.52EE62 pKa = 3.85VTSYY66 pKa = 10.73IGGEE70 pKa = 3.94GHH72 pKa = 6.6NLQEE76 pKa = 4.28HH77 pKa = 5.6SVVLIRR83 pKa = 11.84GGRR86 pKa = 11.84VKK88 pKa = 10.7DD89 pKa = 3.73LPGVRR94 pKa = 11.84YY95 pKa = 8.13HH96 pKa = 6.92TIRR99 pKa = 11.84GALDD103 pKa = 3.24CAGVKK108 pKa = 9.53DD109 pKa = 4.19RR110 pKa = 11.84KK111 pKa = 9.97KK112 pKa = 10.77SRR114 pKa = 11.84SKK116 pKa = 11.25YY117 pKa = 9.9GLKK120 pKa = 10.21KK121 pKa = 10.67SKK123 pKa = 10.62NN124 pKa = 3.55

Molecular weight: 13.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

613

0

613

196271

37

1411

320.2

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.613 ± 0.093

1.248 ± 0.03

4.194 ± 0.059

5.142 ± 0.086

5.322 ± 0.098

5.443 ± 0.087

2.077 ± 0.034

11.972 ± 0.106

10.574 ± 0.115

9.738 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.039

7.345 ± 0.075

2.984 ± 0.042

3.174 ± 0.041

3.616 ± 0.072

6.454 ± 0.059

4.508 ± 0.053

4.724 ± 0.071

0.912 ± 0.037

3.8 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski