Micromonospora endophytica (Xie et al. 2001) Li et al. 2019

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W2BVC3|A0A2W2BVC3_9ACTN TraG-D_C domain-containing protein OS=Micromonospora endophytica (Xie et al. 2001) Li et al. 2019 OX=515350 GN=C1I93_21855 PE=4 SV=1
MM1 pKa = 7.45TISNASALDD10 pKa = 3.41GMNAILFQEE19 pKa = 4.35NPVLPSDD26 pKa = 3.79AVTLAWMSKK35 pKa = 7.7MCHH38 pKa = 6.38PSTTIEE44 pKa = 4.11YY45 pKa = 9.74DD46 pKa = 2.69WSIDD50 pKa = 3.57YY51 pKa = 11.05NFVWGQVGTLVPGTQFVAGQILTADD76 pKa = 4.27LTQNNLVTLSYY87 pKa = 11.24VDD89 pKa = 3.68GGFEE93 pKa = 4.28FGPTSSSGKK102 pKa = 9.95NGSLIIKK109 pKa = 9.88EE110 pKa = 4.57DD111 pKa = 3.57GTVPGPGNEE120 pKa = 4.3DD121 pKa = 3.23QGSVGIGMSGAGTFVVPTLTNAGVEE146 pKa = 4.27FDD148 pKa = 4.52PKK150 pKa = 8.02PTYY153 pKa = 9.07WVAFGSFASSEE164 pKa = 4.15VMDD167 pKa = 4.08VSEE170 pKa = 4.59LTQPAQVSYY179 pKa = 10.39PDD181 pKa = 3.68GMTSAKK187 pKa = 10.48AIFDD191 pKa = 3.7GKK193 pKa = 10.42GWSITYY199 pKa = 10.65GNN201 pKa = 3.86

Molecular weight: 21.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W2BLF6|A0A2W2BLF6_9ACTN Heme ABC transporter ATP-binding protein OS=Micromonospora endophytica (Xie et al. 2001) Li et al. 2019 OX=515350 GN=C1I93_27360 PE=4 SV=1
MM1 pKa = 7.34AHH3 pKa = 7.06AGHH6 pKa = 6.5ARR8 pKa = 11.84KK9 pKa = 8.37RR10 pKa = 11.84TPARR14 pKa = 11.84KK15 pKa = 9.26APPPAKK21 pKa = 10.1VAAKK25 pKa = 10.02KK26 pKa = 10.32VATATKK32 pKa = 9.67RR33 pKa = 11.84ATGATAAKK41 pKa = 10.28SPGGKK46 pKa = 7.28PTPTSPRR53 pKa = 11.84TARR56 pKa = 11.84KK57 pKa = 9.2KK58 pKa = 10.55AVAATAAPSRR68 pKa = 11.84RR69 pKa = 11.84PTAKK73 pKa = 9.35ATAAKK78 pKa = 9.37KK79 pKa = 10.55APAGRR84 pKa = 11.84IATASTTRR92 pKa = 11.84TATRR96 pKa = 11.84KK97 pKa = 8.53ATTAKK102 pKa = 10.61ALAKK106 pKa = 10.4KK107 pKa = 8.93ATTKK111 pKa = 10.41RR112 pKa = 11.84APTAKK117 pKa = 9.58TAATQAAASRR127 pKa = 11.84SRR129 pKa = 11.84SASTTPARR137 pKa = 11.84KK138 pKa = 8.21ATTMEE143 pKa = 3.95SFGNRR148 pKa = 11.84RR149 pKa = 11.84GARR152 pKa = 11.84TGSRR156 pKa = 3.29

Molecular weight: 15.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5896

0

5896

1875034

28

3945

318.0

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.773 ± 0.049

0.753 ± 0.009

5.978 ± 0.022

5.133 ± 0.032

2.664 ± 0.017

9.209 ± 0.028

2.168 ± 0.016

3.365 ± 0.022

1.606 ± 0.02

10.595 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.594 ± 0.013

1.886 ± 0.022

6.249 ± 0.028

2.932 ± 0.019

8.446 ± 0.04

4.77 ± 0.026

6.236 ± 0.031

8.956 ± 0.028

1.592 ± 0.014

2.096 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski