Arthrobacter phage Nandita

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KI73|A0A3G2KI73_9CAUD Uncharacterized protein OS=Arthrobacter phage Nandita OX=2419963 GN=45 PE=4 SV=1
MM1 pKa = 7.85CSLQEE6 pKa = 4.24HH7 pKa = 6.83IEE9 pKa = 4.13RR10 pKa = 11.84DD11 pKa = 3.79VQAQAAAKK19 pKa = 7.98ATQEE23 pKa = 3.76PDD25 pKa = 2.99VPRR28 pKa = 11.84EE29 pKa = 3.85EE30 pKa = 4.46RR31 pKa = 11.84EE32 pKa = 3.76RR33 pKa = 11.84TAYY36 pKa = 9.41WGTCDD41 pKa = 5.01DD42 pKa = 4.49CLTTIVVYY50 pKa = 7.38PHH52 pKa = 7.69PDD54 pKa = 3.27RR55 pKa = 11.84DD56 pKa = 3.56TDD58 pKa = 4.43LPDD61 pKa = 3.95DD62 pKa = 4.48EE63 pKa = 4.77EE64 pKa = 5.11WEE66 pKa = 4.74GGWPDD71 pKa = 4.89CPVCDD76 pKa = 5.6SSLDD80 pKa = 3.66WEE82 pKa = 4.86GTDD85 pKa = 3.63PASHH89 pKa = 7.77IIRR92 pKa = 11.84NYY94 pKa = 9.99AA95 pKa = 2.96

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KI50|A0A3G2KI50_9CAUD Uncharacterized protein OS=Arthrobacter phage Nandita OX=2419963 GN=46 PE=4 SV=1
MM1 pKa = 6.31NHH3 pKa = 5.7EE4 pKa = 4.55RR5 pKa = 11.84IKK7 pKa = 10.64RR8 pKa = 11.84DD9 pKa = 3.7CQCPRR14 pKa = 11.84ANHH17 pKa = 4.82THH19 pKa = 5.86GTPTAYY25 pKa = 10.68VIDD28 pKa = 3.93KK29 pKa = 9.95CRR31 pKa = 11.84CTPCTEE37 pKa = 4.11ANTARR42 pKa = 11.84EE43 pKa = 4.29QARR46 pKa = 11.84QAAKK50 pKa = 10.51KK51 pKa = 10.42AGTYY55 pKa = 10.14DD56 pKa = 3.23AGRR59 pKa = 11.84TDD61 pKa = 3.83AEE63 pKa = 4.26PVRR66 pKa = 11.84THH68 pKa = 5.75IQQLRR73 pKa = 11.84TRR75 pKa = 11.84GYY77 pKa = 10.43GLKK80 pKa = 10.31QIAKK84 pKa = 9.09LARR87 pKa = 11.84VSQSTLGKK95 pKa = 9.64IIYY98 pKa = 9.69GDD100 pKa = 3.91PSRR103 pKa = 11.84NMPPRR108 pKa = 11.84ARR110 pKa = 11.84VEE112 pKa = 3.7KK113 pKa = 10.4HH114 pKa = 4.23VADD117 pKa = 3.71RR118 pKa = 11.84VLAIRR123 pKa = 11.84PSLTTVGEE131 pKa = 4.42TIHH134 pKa = 6.36VKK136 pKa = 10.89AEE138 pKa = 3.68ATQARR143 pKa = 11.84IRR145 pKa = 11.84TLVCLGYY152 pKa = 10.37SINWQAQRR160 pKa = 11.84LGKK163 pKa = 10.43INGNFARR170 pKa = 11.84VLHH173 pKa = 6.34NDD175 pKa = 3.02QVNAKK180 pKa = 6.15TARR183 pKa = 11.84EE184 pKa = 3.95VRR186 pKa = 11.84DD187 pKa = 3.99LYY189 pKa = 11.58LLLWDD194 pKa = 5.46KK195 pKa = 10.68PRR197 pKa = 11.84QPANRR202 pKa = 11.84HH203 pKa = 3.94EE204 pKa = 4.17AAAISRR210 pKa = 11.84AIKK213 pKa = 9.94YY214 pKa = 9.86AADD217 pKa = 4.21RR218 pKa = 11.84GWTQLPPPHH227 pKa = 6.42LTGAEE232 pKa = 3.98APVNPDD238 pKa = 2.58KK239 pKa = 11.28SINAFLEE246 pKa = 3.9QRR248 pKa = 11.84LNRR251 pKa = 11.84QTKK254 pKa = 9.64RR255 pKa = 11.84ISRR258 pKa = 11.84MNEE261 pKa = 3.43RR262 pKa = 11.84KK263 pKa = 8.99PWSTTITATNTTTSAQAPHH282 pKa = 6.74HH283 pKa = 6.96PGTARR288 pKa = 11.84RR289 pKa = 11.84TAHH292 pKa = 6.6PAAPARR298 pKa = 11.84PSGTAPPTSISAA310 pKa = 3.52

Molecular weight: 34.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13468

47

1604

204.1

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.667 ± 0.705

0.676 ± 0.136

5.873 ± 0.298

5.836 ± 0.451

2.777 ± 0.196

8.361 ± 0.397

1.916 ± 0.206

4.344 ± 0.209

4.084 ± 0.26

8.353 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.112

3.193 ± 0.197

5.843 ± 0.371

3.623 ± 0.257

6.326 ± 0.384

5.591 ± 0.204

7.121 ± 0.374

7.41 ± 0.297

1.893 ± 0.126

2.146 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski