Nitrosomonas sp. AL212

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9ZJ06|F9ZJ06_9PROT Flagellar transcriptional regulator FlhC OS=Nitrosomonas sp. AL212 OX=153948 GN=flhC PE=3 SV=1
MM1 pKa = 7.48SFHH4 pKa = 7.2LFHH7 pKa = 8.03INEE10 pKa = 4.22VFSNATGTVQFIEE23 pKa = 4.6FVGDD27 pKa = 3.55ANIQHH32 pKa = 6.53FWAGHH37 pKa = 5.37SIISTNDD44 pKa = 2.69ITSNTYY50 pKa = 10.87SFGTDD55 pKa = 3.15LPGSATAGKK64 pKa = 9.88AVLIATQGFADD75 pKa = 4.67LGIVAPDD82 pKa = 3.58YY83 pKa = 10.68IIPDD87 pKa = 3.61GFLFTTNGAINFPGMIGGTISYY109 pKa = 10.05VALPVDD115 pKa = 4.08GTTSLNRR122 pKa = 11.84DD123 pKa = 2.72GSTGVNSPTNFVGNTGTIFSNVISGTNGADD153 pKa = 3.34NLTGTPGADD162 pKa = 3.49IINAGDD168 pKa = 3.65GLDD171 pKa = 3.52RR172 pKa = 11.84LNGVGGNDD180 pKa = 3.44TLEE183 pKa = 4.62GGLGIDD189 pKa = 3.37TAIYY193 pKa = 8.99SGNRR197 pKa = 11.84VGYY200 pKa = 8.22TIATTSSGFNISGSEE215 pKa = 4.02GDD217 pKa = 4.01DD218 pKa = 3.27TLSGIEE224 pKa = 4.02RR225 pKa = 11.84LQFADD230 pKa = 3.59TKK232 pKa = 11.19LAMDD236 pKa = 5.15LNNGQAANNTARR248 pKa = 11.84IIGAAFGVPAITEE261 pKa = 4.02HH262 pKa = 7.08PDD264 pKa = 3.23YY265 pKa = 10.95VTIGLNLFDD274 pKa = 4.58SGQTVLEE281 pKa = 4.23VSEE284 pKa = 4.47LAVNVLDD291 pKa = 4.63LSNDD295 pKa = 3.45EE296 pKa = 4.86FVDD299 pKa = 3.73AVYY302 pKa = 10.94QNVVGAVPAPAVHH315 pKa = 6.97DD316 pKa = 4.15FYY318 pKa = 12.04VSLLQGSGGSSTQAQLMEE336 pKa = 4.82IGANSVEE343 pKa = 4.1NALNIDD349 pKa = 4.05LAGLAQNGVVFII361 pKa = 4.97

Molecular weight: 37.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9ZIG7|F9ZIG7_9PROT Methylated-DNA--[protein]-cysteine S-methyltransferase OS=Nitrosomonas sp. AL212 OX=153948 GN=NAL212_2725 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.86GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGAAIIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2841

0

2841

886936

27

2886

312.2

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.7 ± 0.053

0.984 ± 0.017

5.344 ± 0.043

5.898 ± 0.047

4.101 ± 0.033

6.93 ± 0.068

2.464 ± 0.026

7.282 ± 0.043

4.941 ± 0.052

10.393 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.024

4.417 ± 0.042

4.165 ± 0.03

4.353 ± 0.037

5.337 ± 0.043

6.266 ± 0.038

5.41 ± 0.042

6.389 ± 0.04

1.255 ± 0.017

2.894 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski