Sphingomonas sp. Root241

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8XKD7|A0A0Q8XKD7_9SPHN Uncharacterized protein OS=Sphingomonas sp. Root241 OX=1736501 GN=ASE13_09965 PE=4 SV=1
MM1 pKa = 7.75PAPAFINEE9 pKa = 3.72IHH11 pKa = 6.67YY12 pKa = 11.02DD13 pKa = 3.48NAGADD18 pKa = 3.16SGEE21 pKa = 4.29FIEE24 pKa = 4.58IAGIAGTDD32 pKa = 3.36LTGWKK37 pKa = 9.47IVLYY41 pKa = 10.42NGANGASYY49 pKa = 10.92NPVMTLSGVIANQQNGFGTISVAYY73 pKa = 7.04PTDD76 pKa = 5.04GIQNGSPDD84 pKa = 3.6AVALVDD90 pKa = 3.72ASNNVVQFLSYY101 pKa = 10.23EE102 pKa = 4.25GSFTATNGPAAGLTSVNVGVSEE124 pKa = 4.59NGAQSGTSIGLVGSGATYY142 pKa = 11.04EE143 pKa = 4.1DD144 pKa = 4.15FHH146 pKa = 6.57WALINGSTAGGVNAGQSFNGVVPPQPGTLSIADD179 pKa = 3.55ATTVEE184 pKa = 4.95GNSGTHH190 pKa = 6.12EE191 pKa = 3.9IVFTVTRR198 pKa = 11.84ADD200 pKa = 3.54GSAGAVSATWTVAFGSADD218 pKa = 3.41AADD221 pKa = 5.0FGAGFTATGTVSFADD236 pKa = 3.79GATTAEE242 pKa = 3.84IRR244 pKa = 11.84LPVQGDD250 pKa = 3.56TAFEE254 pKa = 4.53SNDD257 pKa = 2.94GFTVRR262 pKa = 11.84LSAPQGGVALGDD274 pKa = 3.46AVAAGTITNDD284 pKa = 3.16AAAPPAPPANVFINEE299 pKa = 3.67IHH301 pKa = 6.64YY302 pKa = 11.22DD303 pKa = 3.62NAGTDD308 pKa = 3.05AGEE311 pKa = 4.54AIEE314 pKa = 4.2IAGAAGTDD322 pKa = 3.33LTGYY326 pKa = 10.95KK327 pKa = 10.2LVFYY331 pKa = 10.45NGSNTPGAAPVYY343 pKa = 8.55DD344 pKa = 3.88TLALSGVIDD353 pKa = 4.37DD354 pKa = 4.55EE355 pKa = 5.44SNGFGALGFLRR366 pKa = 11.84AGIQNGAADD375 pKa = 4.78GVALIAPDD383 pKa = 3.82GSVVQLLSYY392 pKa = 10.22EE393 pKa = 4.46GSFTAAAGTPAAGTTSTDD411 pKa = 2.58IGVTEE416 pKa = 4.77EE417 pKa = 3.97PAPAAGLSLQLKK429 pKa = 10.11GSGSSAADD437 pKa = 3.63FAWADD442 pKa = 3.4ASDD445 pKa = 4.28DD446 pKa = 4.11SFGSLNAGQSFLSATGTGHH465 pKa = 7.31LRR467 pKa = 11.84IGDD470 pKa = 3.69ARR472 pKa = 11.84VVEE475 pKa = 4.64GDD477 pKa = 3.25SGTTNLVFTVNRR489 pKa = 11.84AGGTALAATVDD500 pKa = 3.81YY501 pKa = 10.91AVNLDD506 pKa = 3.74GTATLADD513 pKa = 4.51LAPGAVLSGTLSFAPGEE530 pKa = 3.96FSKK533 pKa = 11.09QIVVAVNGDD542 pKa = 3.77LVGEE546 pKa = 4.18PNEE549 pKa = 4.18TLSVSLGATTGNVVIDD565 pKa = 4.1DD566 pKa = 4.05AAATGTITNDD576 pKa = 3.17DD577 pKa = 4.98PIALTISQIQGAGHH591 pKa = 5.89VSAYY595 pKa = 10.14AGQVVITTGIVTAVDD610 pKa = 3.38TNGFYY615 pKa = 10.84LQSAVGDD622 pKa = 3.77GDD624 pKa = 4.29AATSDD629 pKa = 3.29AVFVFTSTAPGVVMGDD645 pKa = 3.58GVSVRR650 pKa = 11.84GSVAEE655 pKa = 4.23FQGSTASLSLTEE667 pKa = 4.35IVAPTVTVEE676 pKa = 3.88THH678 pKa = 6.27GNALPTALLIGTGGILPPSEE698 pKa = 5.17VIDD701 pKa = 4.65DD702 pKa = 5.03DD703 pKa = 5.02GLTSYY708 pKa = 11.34DD709 pKa = 4.01PLHH712 pKa = 7.16DD713 pKa = 5.28GVDD716 pKa = 3.99FWEE719 pKa = 4.5SLEE722 pKa = 3.93GMRR725 pKa = 11.84VTLDD729 pKa = 3.19TPQAVSNTTSFGEE742 pKa = 3.97TDD744 pKa = 3.56VVVSHH749 pKa = 6.65GNGASGINDD758 pKa = 3.41RR759 pKa = 11.84GGITISGDD767 pKa = 3.09GSGVPDD773 pKa = 3.85YY774 pKa = 11.02NPEE777 pKa = 4.5KK778 pKa = 10.6IQIDD782 pKa = 3.88DD783 pKa = 3.97DD784 pKa = 4.42SGIFAGFTPGYY795 pKa = 9.03TIGDD799 pKa = 3.71QLSSVTGVVNYY810 pKa = 10.68AFDD813 pKa = 3.98AYY815 pKa = 10.06EE816 pKa = 4.08VLVTEE821 pKa = 5.03AVTVTQDD828 pKa = 2.95TTLTRR833 pKa = 11.84EE834 pKa = 3.85QTALHH839 pKa = 6.73GDD841 pKa = 3.43ANYY844 pKa = 10.99LSLATYY850 pKa = 10.56NLEE853 pKa = 4.22NLDD856 pKa = 4.69ASDD859 pKa = 3.53QKK861 pKa = 11.32FDD863 pKa = 3.71ILAADD868 pKa = 3.48IVYY871 pKa = 10.61NLRR874 pKa = 11.84APDD877 pKa = 4.15IIAVQEE883 pKa = 3.97IQDD886 pKa = 3.6ADD888 pKa = 3.94GAGSGSNLSGTVTAQGLIDD907 pKa = 5.58AIYY910 pKa = 9.35AQSGLHH916 pKa = 5.08YY917 pKa = 9.92AYY919 pKa = 9.82IEE921 pKa = 4.15IAPTTAGSTGGEE933 pKa = 3.67PGGNIRR939 pKa = 11.84NGYY942 pKa = 9.03FYY944 pKa = 10.92NIDD947 pKa = 3.16RR948 pKa = 11.84VSYY951 pKa = 11.01VEE953 pKa = 5.82GSAQLIDD960 pKa = 3.28GAAYY964 pKa = 10.35NGTRR968 pKa = 11.84KK969 pKa = 9.67PLVAQFAFAGQTITTIDD986 pKa = 3.21VHH988 pKa = 5.32LTSRR992 pKa = 11.84LGSDD996 pKa = 4.19PLWGDD1001 pKa = 3.35NQPANDD1007 pKa = 4.34AGDD1010 pKa = 3.79AARR1013 pKa = 11.84TAQAAGVKK1021 pKa = 10.03AWVQDD1026 pKa = 3.73HH1027 pKa = 6.65LADD1030 pKa = 4.35NPALNIAVLGDD1041 pKa = 3.21WNGFYY1046 pKa = 10.63FEE1048 pKa = 4.81HH1049 pKa = 7.05AQTQLTDD1056 pKa = 3.64PAQGGVFTNLNTLLPEE1072 pKa = 3.99QEE1074 pKa = 4.27RR1075 pKa = 11.84YY1076 pKa = 10.64SYY1078 pKa = 10.2MFEE1081 pKa = 4.59GNAQQIDD1088 pKa = 4.27NILVTGGLVTNAQYY1102 pKa = 11.32DD1103 pKa = 4.06AVHH1106 pKa = 6.61LNSQFGGSRR1115 pKa = 11.84ATDD1118 pKa = 3.46HH1119 pKa = 7.24DD1120 pKa = 4.35PQVSLLFLGAAPKK1133 pKa = 10.57DD1134 pKa = 3.59LALSNASVAEE1144 pKa = 3.89NLPAGSVVGTVSATDD1159 pKa = 3.45TANDD1163 pKa = 3.8TLHH1166 pKa = 6.48YY1167 pKa = 10.87ALVDD1171 pKa = 3.46NAGGLFAIDD1180 pKa = 3.62AATGVITTTAPFNHH1194 pKa = 6.59EE1195 pKa = 4.0ALAEE1199 pKa = 3.98YY1200 pKa = 11.02ALIAKK1205 pKa = 8.21ATDD1208 pKa = 3.22SGGLTTQQSFTVAVTDD1224 pKa = 3.74VNEE1227 pKa = 4.46APVAAHH1233 pKa = 6.95DD1234 pKa = 3.76AVAVNEE1240 pKa = 4.42DD1241 pKa = 3.41ATTANLWTTLLGNDD1255 pKa = 3.82SDD1257 pKa = 4.73PDD1259 pKa = 3.67VGQTLAISAVDD1270 pKa = 3.32ATGTLGSLVFDD1281 pKa = 4.67AASHH1285 pKa = 4.83TLKK1288 pKa = 10.89YY1289 pKa = 10.59VADD1292 pKa = 4.12ADD1294 pKa = 4.49AFDD1297 pKa = 4.29SLAPGATQVDD1307 pKa = 4.09HH1308 pKa = 7.21FSYY1311 pKa = 10.4TVTDD1315 pKa = 4.17GNGLASTATVDD1326 pKa = 3.52VTVTGIADD1334 pKa = 5.29GITRR1338 pKa = 11.84NGTIFSDD1345 pKa = 3.9TLNGSAGEE1353 pKa = 4.28DD1354 pKa = 3.32RR1355 pKa = 11.84LSGGIGSDD1363 pKa = 3.1TLYY1366 pKa = 11.44GLGGHH1371 pKa = 6.96DD1372 pKa = 4.0WLNGGIGNDD1381 pKa = 3.52KK1382 pKa = 10.96LYY1384 pKa = 11.17GGDD1387 pKa = 3.74GNDD1390 pKa = 3.7VLFGDD1395 pKa = 5.33LGNDD1399 pKa = 3.85TLWGGNGRR1407 pKa = 11.84DD1408 pKa = 3.61VLFGGLGNDD1417 pKa = 3.67TLYY1420 pKa = 11.35GGADD1424 pKa = 3.2ADD1426 pKa = 3.89SFHH1429 pKa = 7.25FGRR1432 pKa = 11.84LEE1434 pKa = 4.12GSATIADD1441 pKa = 3.81FNIAEE1446 pKa = 4.48DD1447 pKa = 5.23KK1448 pKa = 10.83IVLDD1452 pKa = 4.71DD1453 pKa = 4.22GVSVTRR1459 pKa = 11.84TKK1461 pKa = 10.81VQDD1464 pKa = 3.43VNRR1467 pKa = 11.84DD1468 pKa = 3.31GVKK1471 pKa = 10.66DD1472 pKa = 3.62LTLTLSWGSSVTLLGVSDD1490 pKa = 4.33AAQVKK1495 pKa = 10.03YY1496 pKa = 10.27GAPDD1500 pKa = 3.39HH1501 pKa = 6.92YY1502 pKa = 11.02SDD1504 pKa = 4.36HH1505 pKa = 6.37QPGLGGFLDD1514 pKa = 5.42DD1515 pKa = 5.61IGDD1518 pKa = 4.25LFDD1521 pKa = 4.1SLYY1524 pKa = 11.08AGHH1527 pKa = 6.47QKK1529 pKa = 10.9LIDD1532 pKa = 3.74TGWFF1536 pKa = 3.29

Molecular weight: 155.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8XRY6|A0A0Q8XRY6_9SPHN Exopolyphosphatase OS=Sphingomonas sp. Root241 OX=1736501 GN=ASE13_00070 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3715

0

3715

1245473

41

5545

335.3

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.37 ± 0.068

0.67 ± 0.013

5.783 ± 0.03

5.388 ± 0.054

3.627 ± 0.027

9.239 ± 0.085

1.862 ± 0.022

4.836 ± 0.027

2.907 ± 0.038

9.875 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.023

2.765 ± 0.052

5.399 ± 0.045

3.083 ± 0.026

7.261 ± 0.053

5.21 ± 0.041

5.559 ± 0.068

7.199 ± 0.031

1.488 ± 0.016

2.263 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski