Amino acid dipepetide frequency for Mycobacterium phage Phrann

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.174AlaAla: 18.174 ± 2.819
1.106AlaCys: 1.106 ± 0.393
8.223AlaAsp: 8.223 ± 0.709
7.878AlaGlu: 7.878 ± 0.8
3.455AlaPhe: 3.455 ± 0.551
9.26AlaGly: 9.26 ± 1.443
2.35AlaHis: 2.35 ± 0.499
6.15AlaIle: 6.15 ± 0.697
3.041AlaLys: 3.041 ± 0.603
9.675AlaLeu: 9.675 ± 0.928
2.142AlaMet: 2.142 ± 0.413
3.662AlaAsn: 3.662 ± 0.522
6.427AlaPro: 6.427 ± 0.787
4.837AlaGln: 4.837 ± 0.546
7.947AlaArg: 7.947 ± 0.898
6.081AlaSer: 6.081 ± 0.699
6.91AlaThr: 6.91 ± 0.771
7.671AlaVal: 7.671 ± 0.768
2.211AlaTrp: 2.211 ± 0.374
2.557AlaTyr: 2.557 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
1.244CysAla: 1.244 ± 0.391
0.138CysCys: 0.138 ± 0.094
0.829CysAsp: 0.829 ± 0.232
0.898CysGlu: 0.898 ± 0.279
0.0CysPhe: 0.0 ± 0.0
1.175CysGly: 1.175 ± 0.389
0.138CysHis: 0.138 ± 0.1
0.207CysIle: 0.207 ± 0.115
0.415CysLys: 0.415 ± 0.162
0.622CysLeu: 0.622 ± 0.183
0.138CysMet: 0.138 ± 0.094
0.276CysAsn: 0.276 ± 0.116
0.898CysPro: 0.898 ± 0.327
0.415CysGln: 0.415 ± 0.209
0.691CysArg: 0.691 ± 0.256
0.415CysSer: 0.415 ± 0.174
1.037CysThr: 1.037 ± 0.308
0.691CysVal: 0.691 ± 0.237
0.069CysTrp: 0.069 ± 0.066
0.138CysTyr: 0.138 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
7.463AspAla: 7.463 ± 0.587
1.037AspCys: 1.037 ± 0.323
5.805AspAsp: 5.805 ± 0.701
5.39AspGlu: 5.39 ± 0.674
1.797AspPhe: 1.797 ± 0.373
6.496AspGly: 6.496 ± 0.743
1.382AspHis: 1.382 ± 0.289
2.488AspIle: 2.488 ± 0.285
1.797AspLys: 1.797 ± 0.388
6.841AspLeu: 6.841 ± 0.709
1.451AspMet: 1.451 ± 0.298
1.451AspAsn: 1.451 ± 0.311
4.284AspPro: 4.284 ± 0.658
1.52AspGln: 1.52 ± 0.256
4.423AspArg: 4.423 ± 0.608
3.801AspSer: 3.801 ± 0.696
2.902AspThr: 2.902 ± 0.469
3.939AspVal: 3.939 ± 0.55
1.313AspTrp: 1.313 ± 0.309
1.52AspTyr: 1.52 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
6.358GluAla: 6.358 ± 0.683
0.76GluCys: 0.76 ± 0.196
3.939GluAsp: 3.939 ± 0.45
2.695GluGlu: 2.695 ± 0.418
2.488GluPhe: 2.488 ± 0.463
2.488GluGly: 2.488 ± 0.352
1.451GluHis: 1.451 ± 0.345
3.317GluIle: 3.317 ± 0.408
2.28GluLys: 2.28 ± 0.324
6.012GluLeu: 6.012 ± 0.741
1.589GluMet: 1.589 ± 0.35
1.728GluAsn: 1.728 ± 0.277
2.419GluPro: 2.419 ± 0.477
3.386GluGln: 3.386 ± 0.505
4.561GluArg: 4.561 ± 0.608
2.971GluSer: 2.971 ± 0.505
4.354GluThr: 4.354 ± 0.575
4.906GluVal: 4.906 ± 0.766
1.382GluTrp: 1.382 ± 0.317
1.52GluTyr: 1.52 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.939PheAla: 3.939 ± 0.497
0.207PheCys: 0.207 ± 0.156
2.28PheAsp: 2.28 ± 0.531
1.52PheGlu: 1.52 ± 0.364
0.829PhePhe: 0.829 ± 0.297
3.317PheGly: 3.317 ± 0.503
0.967PheHis: 0.967 ± 0.302
1.313PheIle: 1.313 ± 0.308
0.76PheLys: 0.76 ± 0.208
1.451PheLeu: 1.451 ± 0.334
0.415PheMet: 0.415 ± 0.161
1.106PheAsn: 1.106 ± 0.217
0.898PhePro: 0.898 ± 0.291
0.76PheGln: 0.76 ± 0.208
1.658PheArg: 1.658 ± 0.319
1.451PheSer: 1.451 ± 0.276
2.35PheThr: 2.35 ± 0.381
2.35PheVal: 2.35 ± 0.395
0.622PheTrp: 0.622 ± 0.289
0.829PheTyr: 0.829 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
8.223GlyAla: 8.223 ± 1.129
0.622GlyCys: 0.622 ± 0.201
5.114GlyAsp: 5.114 ± 0.679
4.906GlyGlu: 4.906 ± 0.759
2.211GlyPhe: 2.211 ± 0.444
9.882GlyGly: 9.882 ± 1.351
1.313GlyHis: 1.313 ± 0.264
4.906GlyIle: 4.906 ± 0.631
2.211GlyLys: 2.211 ± 0.352
6.427GlyLeu: 6.427 ± 0.596
1.52GlyMet: 1.52 ± 0.286
3.386GlyAsn: 3.386 ± 0.703
4.354GlyPro: 4.354 ± 0.825
4.146GlyGln: 4.146 ± 0.547
5.874GlyArg: 5.874 ± 0.664
5.528GlySer: 5.528 ± 0.6
5.874GlyThr: 5.874 ± 0.68
6.772GlyVal: 6.772 ± 0.72
1.797GlyTrp: 1.797 ± 0.301
3.041GlyTyr: 3.041 ± 0.423
0.0GlyXaa: 0.0 ± 0.0
His
1.589HisAla: 1.589 ± 0.348
0.276HisCys: 0.276 ± 0.161
1.382HisAsp: 1.382 ± 0.269
1.658HisGlu: 1.658 ± 0.386
0.484HisPhe: 0.484 ± 0.194
1.866HisGly: 1.866 ± 0.353
0.898HisHis: 0.898 ± 0.251
0.829HisIle: 0.829 ± 0.217
0.898HisLys: 0.898 ± 0.264
1.589HisLeu: 1.589 ± 0.332
0.207HisMet: 0.207 ± 0.134
0.415HisAsn: 0.415 ± 0.224
1.037HisPro: 1.037 ± 0.259
0.829HisGln: 0.829 ± 0.219
2.419HisArg: 2.419 ± 0.43
1.106HisSer: 1.106 ± 0.276
1.728HisThr: 1.728 ± 0.373
1.52HisVal: 1.52 ± 0.367
0.346HisTrp: 0.346 ± 0.132
0.76HisTyr: 0.76 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
5.114IleAla: 5.114 ± 0.515
0.553IleCys: 0.553 ± 0.234
3.179IleAsp: 3.179 ± 0.447
3.662IleGlu: 3.662 ± 0.434
0.622IlePhe: 0.622 ± 0.208
5.39IleGly: 5.39 ± 0.704
1.244IleHis: 1.244 ± 0.335
1.52IleIle: 1.52 ± 0.275
0.76IleLys: 0.76 ± 0.205
2.764IleLeu: 2.764 ± 0.424
0.553IleMet: 0.553 ± 0.195
1.797IleAsn: 1.797 ± 0.384
2.35IlePro: 2.35 ± 0.386
1.037IleGln: 1.037 ± 0.231
3.593IleArg: 3.593 ± 0.47
2.28IleSer: 2.28 ± 0.414
3.801IleThr: 3.801 ± 0.585
4.146IleVal: 4.146 ± 0.647
0.691IleTrp: 0.691 ± 0.191
0.829IleTyr: 0.829 ± 0.223
0.0IleXaa: 0.0 ± 0.0
Lys
5.045LysAla: 5.045 ± 0.795
0.276LysCys: 0.276 ± 0.145
0.898LysAsp: 0.898 ± 0.248
1.451LysGlu: 1.451 ± 0.257
0.829LysPhe: 0.829 ± 0.207
2.488LysGly: 2.488 ± 0.395
0.553LysHis: 0.553 ± 0.177
1.244LysIle: 1.244 ± 0.257
0.415LysLys: 0.415 ± 0.136
1.866LysLeu: 1.866 ± 0.345
0.898LysMet: 0.898 ± 0.233
0.967LysAsn: 0.967 ± 0.221
2.419LysPro: 2.419 ± 0.411
1.175LysGln: 1.175 ± 0.312
2.35LysArg: 2.35 ± 0.422
1.52LysSer: 1.52 ± 0.299
1.797LysThr: 1.797 ± 0.345
2.004LysVal: 2.004 ± 0.383
0.346LysTrp: 0.346 ± 0.128
0.829LysTyr: 0.829 ± 0.19
0.0LysXaa: 0.0 ± 0.0
Leu
11.126LeuAla: 11.126 ± 0.909
0.622LeuCys: 0.622 ± 0.221
5.114LeuAsp: 5.114 ± 0.534
3.662LeuGlu: 3.662 ± 0.387
2.833LeuPhe: 2.833 ± 0.565
8.5LeuGly: 8.5 ± 0.79
1.589LeuHis: 1.589 ± 0.304
3.593LeuIle: 3.593 ± 0.447
3.248LeuLys: 3.248 ± 0.462
6.15LeuLeu: 6.15 ± 0.686
1.244LeuMet: 1.244 ± 0.37
2.142LeuAsn: 2.142 ± 0.432
4.146LeuPro: 4.146 ± 0.596
2.557LeuGln: 2.557 ± 0.448
5.252LeuArg: 5.252 ± 0.624
4.492LeuSer: 4.492 ± 0.524
4.837LeuThr: 4.837 ± 0.605
5.252LeuVal: 5.252 ± 0.49
1.175LeuTrp: 1.175 ± 0.285
1.313LeuTyr: 1.313 ± 0.316
0.0LeuXaa: 0.0 ± 0.0
Met
2.764MetAla: 2.764 ± 0.41
0.0MetCys: 0.0 ± 0.0
0.898MetAsp: 0.898 ± 0.303
0.691MetGlu: 0.691 ± 0.233
0.829MetPhe: 0.829 ± 0.253
0.553MetGly: 0.553 ± 0.15
0.553MetHis: 0.553 ± 0.155
0.829MetIle: 0.829 ± 0.249
0.76MetLys: 0.76 ± 0.228
2.073MetLeu: 2.073 ± 0.338
0.622MetMet: 0.622 ± 0.225
0.622MetAsn: 0.622 ± 0.172
1.451MetPro: 1.451 ± 0.294
0.829MetGln: 0.829 ± 0.222
1.037MetArg: 1.037 ± 0.299
2.28MetSer: 2.28 ± 0.308
2.004MetThr: 2.004 ± 0.378
0.898MetVal: 0.898 ± 0.211
0.691MetTrp: 0.691 ± 0.233
0.207MetTyr: 0.207 ± 0.097
0.0MetXaa: 0.0 ± 0.0
Asn
2.764AsnAla: 2.764 ± 0.625
0.415AsnCys: 0.415 ± 0.159
1.797AsnAsp: 1.797 ± 0.31
0.829AsnGlu: 0.829 ± 0.222
1.037AsnPhe: 1.037 ± 0.209
4.008AsnGly: 4.008 ± 0.618
0.553AsnHis: 0.553 ± 0.159
1.658AsnIle: 1.658 ± 0.48
0.622AsnLys: 0.622 ± 0.257
2.073AsnLeu: 2.073 ± 0.418
0.415AsnMet: 0.415 ± 0.198
1.244AsnAsn: 1.244 ± 0.284
2.695AsnPro: 2.695 ± 0.365
1.106AsnGln: 1.106 ± 0.292
2.28AsnArg: 2.28 ± 0.381
1.037AsnSer: 1.037 ± 0.236
1.866AsnThr: 1.866 ± 0.298
2.28AsnVal: 2.28 ± 0.338
0.484AsnTrp: 0.484 ± 0.173
0.622AsnTyr: 0.622 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
7.671ProAla: 7.671 ± 0.902
0.76ProCys: 0.76 ± 0.234
4.215ProAsp: 4.215 ± 0.534
4.077ProGlu: 4.077 ± 0.595
2.28ProPhe: 2.28 ± 0.368
6.15ProGly: 6.15 ± 0.73
0.967ProHis: 0.967 ± 0.285
2.004ProIle: 2.004 ± 0.402
1.382ProLys: 1.382 ± 0.366
4.008ProLeu: 4.008 ± 0.665
1.175ProMet: 1.175 ± 0.301
1.658ProAsn: 1.658 ± 0.332
2.695ProPro: 2.695 ± 0.408
2.073ProGln: 2.073 ± 0.317
3.041ProArg: 3.041 ± 0.518
2.833ProSer: 2.833 ± 0.43
3.524ProThr: 3.524 ± 0.538
3.662ProVal: 3.662 ± 0.523
1.451ProTrp: 1.451 ± 0.345
1.037ProTyr: 1.037 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
5.183GlnAla: 5.183 ± 0.908
0.346GlnCys: 0.346 ± 0.158
1.935GlnAsp: 1.935 ± 0.432
1.313GlnGlu: 1.313 ± 0.304
1.106GlnPhe: 1.106 ± 0.237
1.451GlnGly: 1.451 ± 0.343
1.106GlnHis: 1.106 ± 0.245
2.004GlnIle: 2.004 ± 0.313
0.898GlnLys: 0.898 ± 0.286
4.146GlnLeu: 4.146 ± 0.52
0.967GlnMet: 0.967 ± 0.226
0.484GlnAsn: 0.484 ± 0.205
2.419GlnPro: 2.419 ± 0.414
1.935GlnGln: 1.935 ± 0.386
3.593GlnArg: 3.593 ± 0.608
1.728GlnSer: 1.728 ± 0.329
2.557GlnThr: 2.557 ± 0.365
2.833GlnVal: 2.833 ± 0.418
0.553GlnTrp: 0.553 ± 0.19
0.829GlnTyr: 0.829 ± 0.253
0.0GlnXaa: 0.0 ± 0.0
Arg
6.91ArgAla: 6.91 ± 0.677
0.691ArgCys: 0.691 ± 0.235
5.39ArgAsp: 5.39 ± 0.647
5.114ArgGlu: 5.114 ± 0.753
1.866ArgPhe: 1.866 ± 0.349
4.768ArgGly: 4.768 ± 0.627
1.866ArgHis: 1.866 ± 0.338
3.87ArgIle: 3.87 ± 0.623
2.211ArgLys: 2.211 ± 0.394
5.874ArgLeu: 5.874 ± 0.611
2.004ArgMet: 2.004 ± 0.498
1.728ArgAsn: 1.728 ± 0.314
3.939ArgPro: 3.939 ± 0.471
2.764ArgGln: 2.764 ± 0.578
6.496ArgArg: 6.496 ± 0.813
3.524ArgSer: 3.524 ± 0.549
4.215ArgThr: 4.215 ± 0.484
4.215ArgVal: 4.215 ± 0.551
1.589ArgTrp: 1.589 ± 0.332
2.488ArgTyr: 2.488 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
6.219SerAla: 6.219 ± 0.859
0.346SerCys: 0.346 ± 0.165
3.455SerAsp: 3.455 ± 0.423
2.142SerGlu: 2.142 ± 0.321
1.589SerPhe: 1.589 ± 0.342
6.081SerGly: 6.081 ± 0.988
0.691SerHis: 0.691 ± 0.197
1.728SerIle: 1.728 ± 0.265
1.935SerLys: 1.935 ± 0.355
4.008SerLeu: 4.008 ± 0.613
2.488SerMet: 2.488 ± 0.429
1.313SerAsn: 1.313 ± 0.365
3.662SerPro: 3.662 ± 0.404
1.589SerGln: 1.589 ± 0.456
3.386SerArg: 3.386 ± 0.455
2.626SerSer: 2.626 ± 0.344
3.732SerThr: 3.732 ± 0.457
3.11SerVal: 3.11 ± 0.47
0.967SerTrp: 0.967 ± 0.195
1.658SerTyr: 1.658 ± 0.272
0.0SerXaa: 0.0 ± 0.0
Thr
8.154ThrAla: 8.154 ± 0.582
0.622ThrCys: 0.622 ± 0.236
4.699ThrAsp: 4.699 ± 0.572
3.386ThrGlu: 3.386 ± 0.377
1.658ThrPhe: 1.658 ± 0.377
5.943ThrGly: 5.943 ± 0.699
1.313ThrHis: 1.313 ± 0.329
3.179ThrIle: 3.179 ± 0.487
2.073ThrLys: 2.073 ± 0.315
6.012ThrLeu: 6.012 ± 0.584
0.967ThrMet: 0.967 ± 0.211
1.589ThrAsn: 1.589 ± 0.331
4.975ThrPro: 4.975 ± 0.715
2.142ThrGln: 2.142 ± 0.476
4.561ThrArg: 4.561 ± 0.609
2.764ThrSer: 2.764 ± 0.586
3.455ThrThr: 3.455 ± 0.674
6.081ThrVal: 6.081 ± 0.763
0.553ThrTrp: 0.553 ± 0.205
1.935ThrTyr: 1.935 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
8.223ValAla: 8.223 ± 0.647
0.829ValCys: 0.829 ± 0.248
5.252ValAsp: 5.252 ± 0.769
6.219ValGlu: 6.219 ± 0.645
1.797ValPhe: 1.797 ± 0.327
5.045ValGly: 5.045 ± 0.451
1.52ValHis: 1.52 ± 0.394
3.455ValIle: 3.455 ± 0.487
2.28ValLys: 2.28 ± 0.462
3.662ValLeu: 3.662 ± 0.451
1.313ValMet: 1.313 ± 0.362
2.764ValAsn: 2.764 ± 0.563
4.077ValPro: 4.077 ± 0.459
2.764ValGln: 2.764 ± 0.456
5.39ValArg: 5.39 ± 0.65
3.524ValSer: 3.524 ± 0.739
5.321ValThr: 5.321 ± 0.679
5.528ValVal: 5.528 ± 0.696
1.106ValTrp: 1.106 ± 0.233
1.382ValTyr: 1.382 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
1.52TrpAla: 1.52 ± 0.334
0.415TrpCys: 0.415 ± 0.18
1.175TrpAsp: 1.175 ± 0.256
0.691TrpGlu: 0.691 ± 0.213
0.691TrpPhe: 0.691 ± 0.21
0.967TrpGly: 0.967 ± 0.213
0.898TrpHis: 0.898 ± 0.228
0.76TrpIle: 0.76 ± 0.226
0.553TrpLys: 0.553 ± 0.193
1.658TrpLeu: 1.658 ± 0.388
0.207TrpMet: 0.207 ± 0.127
0.276TrpAsn: 0.276 ± 0.122
0.622TrpPro: 0.622 ± 0.245
0.829TrpGln: 0.829 ± 0.203
1.244TrpArg: 1.244 ± 0.283
1.451TrpSer: 1.451 ± 0.256
1.658TrpThr: 1.658 ± 0.339
1.451TrpVal: 1.451 ± 0.295
0.553TrpTrp: 0.553 ± 0.197
0.484TrpTyr: 0.484 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.073TyrAla: 2.073 ± 0.334
0.415TyrCys: 0.415 ± 0.146
1.52TyrAsp: 1.52 ± 0.341
2.419TyrGlu: 2.419 ± 0.379
0.622TyrPhe: 0.622 ± 0.209
2.28TyrGly: 2.28 ± 0.311
0.484TyrHis: 0.484 ± 0.179
0.829TyrIle: 0.829 ± 0.249
0.829TyrLys: 0.829 ± 0.229
1.935TyrLeu: 1.935 ± 0.393
0.207TyrMet: 0.207 ± 0.111
1.175TyrAsn: 1.175 ± 0.26
0.898TyrPro: 0.898 ± 0.235
0.691TyrGln: 0.691 ± 0.205
1.728TyrArg: 1.728 ± 0.316
1.313TyrSer: 1.313 ± 0.358
2.142TyrThr: 2.142 ± 0.375
2.142TyrVal: 2.142 ± 0.483
0.276TyrTrp: 0.276 ± 0.122
0.76TyrTyr: 0.76 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14472 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski