Ectocarpus siliculosus (Brown alga) (Conferva siliculosa)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; PX clade; Phaeophyceae; Ectocarpales; Ectocarpaceae; Ectocarpus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8LQK9|D8LQK9_ECTSI Uncharacterized protein OS=Ectocarpus siliculosus OX=2880 GN=Esi_0006_0175 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 4.66VVLSPEE8 pKa = 4.18DD9 pKa = 3.58LEE11 pKa = 5.78GYY13 pKa = 8.32PSPGDD18 pKa = 3.33AVVYY22 pKa = 8.92TITITNDD29 pKa = 2.92GSLTLHH35 pKa = 7.04DD36 pKa = 4.45VTPDD40 pKa = 3.34SPQALAWACEE50 pKa = 4.0LAEE53 pKa = 4.51GAALVPGVEE62 pKa = 4.61TKK64 pKa = 10.8CVGRR68 pKa = 11.84LVLTQNNLDD77 pKa = 3.71EE78 pKa = 4.86AQVVTTVTITATDD91 pKa = 3.51SANEE95 pKa = 3.95LVEE98 pKa = 6.09DD99 pKa = 4.6DD100 pKa = 4.55LSSTVEE106 pKa = 4.27LPTSSSLLVVEE117 pKa = 4.94TCSFTGSDD125 pKa = 3.26ANRR128 pKa = 11.84AEE130 pKa = 4.27VGQGVAYY137 pKa = 8.84TFTMKK142 pKa = 9.58NTGSTTLTGLDD153 pKa = 3.71VNSVFLDD160 pKa = 3.48QALDD164 pKa = 3.72NVACGASSLSEE175 pKa = 4.03LARR178 pKa = 11.84DD179 pKa = 3.77DD180 pKa = 4.7TIVCSSVLDD189 pKa = 3.78HH190 pKa = 7.72AITQVSAVASDD201 pKa = 3.25STMVSEE207 pKa = 4.78GVSISTCVDD216 pKa = 4.47GISSLSLGKK225 pKa = 8.45TVVGGGSWVDD235 pKa = 3.53SNGDD239 pKa = 3.37EE240 pKa = 4.2DD241 pKa = 4.11TDD243 pKa = 3.7AGEE246 pKa = 4.3VILYY250 pKa = 9.47QLLVVNVGTVTLNSVVLTDD269 pKa = 4.32GSVTSEE275 pKa = 4.04GVSCEE280 pKa = 3.86SGIPDD285 pKa = 3.62SLLPGEE291 pKa = 4.85GFEE294 pKa = 4.65CQAEE298 pKa = 4.16YY299 pKa = 9.97TIVQDD304 pKa = 3.64DD305 pKa = 3.79VDD307 pKa = 4.01RR308 pKa = 11.84GFVVSNATVTAINPSKK324 pKa = 11.09DD325 pKa = 3.28KK326 pKa = 10.75TNKK329 pKa = 8.34TSEE332 pKa = 3.95VSTDD336 pKa = 3.38LVRR339 pKa = 11.84RR340 pKa = 11.84PAISLDD346 pKa = 4.02TVGSWANAGNGSDD359 pKa = 3.74GFADD363 pKa = 3.81AGDD366 pKa = 3.79TAVYY370 pKa = 9.81RR371 pKa = 11.84YY372 pKa = 7.8TSSEE376 pKa = 3.99PGQSAGCSGLSVLNWAAIEE395 pKa = 4.26AGGLTTVSRR404 pKa = 11.84VHH406 pKa = 6.96SVDD409 pKa = 2.96ATLGLPVPSQTTVSIDD425 pKa = 3.72LPPPPSIQLDD435 pKa = 3.83MVGTFADD442 pKa = 4.41DD443 pKa = 3.98SADD446 pKa = 3.57GMQGLADD453 pKa = 3.8VGEE456 pKa = 4.38MISYY460 pKa = 10.15VFTITNNGHH469 pKa = 6.47AVLEE473 pKa = 5.15GITLADD479 pKa = 3.72GGTNAGISSTTTCGDD494 pKa = 3.22TAIASRR500 pKa = 11.84NASSITVGGTLAVRR514 pKa = 11.84AVITCNSSYY523 pKa = 11.11AITEE527 pKa = 4.31DD528 pKa = 3.3NVNALEE534 pKa = 4.18VSSAASVTASDD545 pKa = 3.81VSGNAVDD552 pKa = 4.77AADD555 pKa = 3.75ATVVSLDD562 pKa = 4.08QINTGTKK569 pKa = 9.49SAGAQVTAWYY579 pKa = 9.77EE580 pKa = 3.95DD581 pKa = 3.77EE582 pKa = 5.02PGNLSEE588 pKa = 4.35EE589 pKa = 4.45TSDD592 pKa = 3.53GTVVDD597 pKa = 4.21VALKK601 pKa = 9.93QDD603 pKa = 3.89PLAGITKK610 pKa = 8.67TFVFTPARR618 pKa = 11.84EE619 pKa = 4.08DD620 pKa = 3.32GMASVDD626 pKa = 3.39DD627 pKa = 4.77TIAYY631 pKa = 7.74TITASNDD638 pKa = 3.26GNVDD642 pKa = 3.45LSDD645 pKa = 3.11ITVTDD650 pKa = 3.56EE651 pKa = 5.52RR652 pKa = 11.84FLNPQGGFDD661 pKa = 4.39MNWEE665 pKa = 4.22SDD667 pKa = 3.42TSFGMLPGAEE677 pKa = 4.01LHH679 pKa = 6.86CYY681 pKa = 6.99PTTAITQADD690 pKa = 3.23IDD692 pKa = 4.2AGVVASNVTHH702 pKa = 7.28PDD704 pKa = 3.0AAIVCTPSLLGLSLAPGAEE723 pKa = 4.5VSCSAFYY730 pKa = 10.03PVSQDD735 pKa = 3.2DD736 pKa = 3.83VNAGVVSSEE745 pKa = 3.89ATISADD751 pKa = 3.66SPIGPISVSNSSEE764 pKa = 4.06PQSLEE769 pKa = 3.72QVDD772 pKa = 5.01GIDD775 pKa = 3.76IGDD778 pKa = 3.94EE779 pKa = 4.2LFCPLEE785 pKa = 4.08GHH787 pKa = 6.41TFTCTAVVHH796 pKa = 5.32VTQEE800 pKa = 3.87NMNDD804 pKa = 3.05GHH806 pKa = 6.99VDD808 pKa = 3.01HH809 pKa = 7.93DD810 pKa = 3.74IGVTAKK816 pKa = 9.6TAIGDD821 pKa = 3.86EE822 pKa = 4.35PLDD825 pKa = 3.71DD826 pKa = 3.9QYY828 pKa = 11.95RR829 pKa = 11.84LHH831 pKa = 6.7VPLEE835 pKa = 4.37GKK837 pKa = 9.55SAFLIEE843 pKa = 4.84HH844 pKa = 6.12NSEE847 pKa = 4.07YY848 pKa = 10.99LPVDD852 pKa = 4.46GIGAASALGDD862 pKa = 4.09EE863 pKa = 4.57ITYY866 pKa = 8.86TLNIDD871 pKa = 3.56NNGTVTLSSVTPVNSKK887 pKa = 10.47VEE889 pKa = 4.21LTCEE893 pKa = 3.9PDD895 pKa = 3.53VNSGAKK901 pKa = 9.86LDD903 pKa = 4.03AGEE906 pKa = 4.65GAMCTGTYY914 pKa = 9.94IVTQDD919 pKa = 4.83DD920 pKa = 3.31IDD922 pKa = 3.86AGKK925 pKa = 9.64IVCAASVTATDD936 pKa = 4.33PDD938 pKa = 3.93GEE940 pKa = 4.84LIFRR944 pKa = 11.84QTRR947 pKa = 11.84ISQHH951 pKa = 5.0VSQTPQLSVVLSSVHH966 pKa = 5.4TVNSLDD972 pKa = 3.25GKK974 pKa = 6.96TRR976 pKa = 11.84KK977 pKa = 9.99GDD979 pKa = 3.44TVLYY983 pKa = 5.85TTQVFNSGNTCLTDD997 pKa = 3.48VKK999 pKa = 10.52ISEE1002 pKa = 4.42LLVDD1006 pKa = 4.78GGLDD1010 pKa = 3.53CGSASSTLCPTDD1022 pKa = 3.41EE1023 pKa = 5.77AISCTGTHH1031 pKa = 6.62TLTQANVDD1039 pKa = 3.77SVHH1042 pKa = 7.24IINTATATASPLFANTSDD1060 pKa = 3.65EE1061 pKa = 4.72SNMISAGDD1069 pKa = 3.8GDD1071 pKa = 4.4TVSWLLYY1078 pKa = 8.75PAISVGSLTSVSLEE1092 pKa = 3.83VLLLEE1097 pKa = 4.46RR1098 pKa = 11.84SGTIVDD1104 pKa = 4.07CTPSVTEE1111 pKa = 4.54ALVLAPGGIVLCSATLEE1128 pKa = 4.19LTQDD1132 pKa = 4.19HH1133 pKa = 7.25IDD1135 pKa = 3.85GGALTSEE1142 pKa = 4.07MFARR1146 pKa = 11.84GVASDD1151 pKa = 3.7GQAVLGEE1158 pKa = 4.3DD1159 pKa = 3.5SVHH1162 pKa = 6.22QEE1164 pKa = 3.8LVQDD1168 pKa = 3.78VGLSVAEE1175 pKa = 3.69IGAFNDD1181 pKa = 3.16EE1182 pKa = 5.17DD1183 pKa = 4.75GDD1185 pKa = 4.02EE1186 pKa = 4.81LGDD1189 pKa = 3.81AGEE1192 pKa = 4.4TMFYY1196 pKa = 10.13TATFRR1201 pKa = 11.84NIGNVRR1207 pKa = 11.84VGNVRR1212 pKa = 11.84VSHH1215 pKa = 5.69LQGHH1219 pKa = 6.52IAALACDD1226 pKa = 3.72SGFEE1230 pKa = 4.54AVTSTDD1236 pKa = 3.0GLGEE1240 pKa = 4.3LLPGIEE1246 pKa = 4.33FGCRR1250 pKa = 11.84ATYY1253 pKa = 10.92SLTQADD1259 pKa = 3.22VDD1261 pKa = 3.65AAEE1264 pKa = 4.46VVNTASISGVARR1276 pKa = 11.84DD1277 pKa = 3.69TSGTEE1282 pKa = 3.91VEE1284 pKa = 6.01AEE1286 pKa = 4.11DD1287 pKa = 3.64SWKK1290 pKa = 10.35QEE1292 pKa = 3.88YY1293 pKa = 7.99TQLAMALLNVVGVFHH1308 pKa = 7.9DD1309 pKa = 3.69VGTVDD1314 pKa = 5.79DD1315 pKa = 4.79EE1316 pKa = 5.69ADD1318 pKa = 2.98IHH1320 pKa = 7.29DD1321 pKa = 3.78KK1322 pKa = 10.7MEE1324 pKa = 4.19YY1325 pKa = 8.96TVEE1328 pKa = 3.85IANTGTVTLTDD1339 pKa = 3.44VGVTGSLFEE1348 pKa = 4.74NEE1350 pKa = 5.34AIPCPGATLAPAEE1363 pKa = 4.39SMVCNASYY1371 pKa = 10.12TITNTDD1377 pKa = 2.55IDD1379 pKa = 4.13RR1380 pKa = 11.84YY1381 pKa = 9.29EE1382 pKa = 4.23VEE1384 pKa = 4.09TTADD1388 pKa = 3.52VTASGPFAQAVGDD1401 pKa = 3.82SGVYY1405 pKa = 9.7LQRR1408 pKa = 11.84LDD1410 pKa = 3.92AQPSVSLLMEE1420 pKa = 4.64GVHH1423 pKa = 6.51VDD1425 pKa = 3.46SQIDD1429 pKa = 3.63STASAEE1435 pKa = 4.13SDD1437 pKa = 3.61GNSVADD1443 pKa = 3.57AGEE1446 pKa = 4.06YY1447 pKa = 8.83TRR1449 pKa = 11.84YY1450 pKa = 8.75TMVVRR1455 pKa = 11.84NTGMLTVHH1463 pKa = 6.72QITVVEE1469 pKa = 4.17SLEE1472 pKa = 4.38GGEE1475 pKa = 4.1ATCPKK1480 pKa = 10.35NYY1482 pKa = 10.75LEE1484 pKa = 4.33MAEE1487 pKa = 4.69SMVCNATYY1495 pKa = 10.49PLTQEE1500 pKa = 4.31DD1501 pKa = 4.11VDD1503 pKa = 4.48RR1504 pKa = 11.84GDD1506 pKa = 3.54VTSVTTLDD1514 pKa = 3.34AFGPTRR1520 pKa = 11.84DD1521 pKa = 3.92DD1522 pKa = 3.63GEE1524 pKa = 4.3ARR1526 pKa = 11.84STTRR1530 pKa = 11.84AEE1532 pKa = 3.69ASSWVKK1538 pKa = 10.58LPQDD1542 pKa = 3.43PSILVTKK1549 pKa = 9.74EE1550 pKa = 3.87CAWQDD1555 pKa = 3.12GAEE1558 pKa = 4.16RR1559 pKa = 11.84DD1560 pKa = 3.96GLPDD1564 pKa = 4.16PGEE1567 pKa = 4.27VVVLIYY1573 pKa = 9.95TVSNTGSVTLTDD1585 pKa = 4.14GSLQQSTDD1593 pKa = 2.99LGLQVVDD1600 pKa = 4.8CMNAIPSAGNVVCTSSIEE1618 pKa = 3.88IGQADD1623 pKa = 3.82IDD1625 pKa = 4.24AGTVTSTVEE1634 pKa = 3.69ITALSPLGDD1643 pKa = 3.65MTGSTTNCSWAWGVLPAQVDD1663 pKa = 3.87TVITGRR1669 pKa = 11.84FVDD1672 pKa = 3.9GDD1674 pKa = 3.7GDD1676 pKa = 4.36GIASVGEE1683 pKa = 3.94GTGYY1687 pKa = 11.04NVTIVNNGKK1696 pKa = 8.35VTVTVVEE1703 pKa = 4.22VDD1705 pKa = 3.22ATAVARR1711 pKa = 11.84GSPSSGNSASGVTIVCEE1728 pKa = 4.07PLLDD1732 pKa = 4.01TSPLLAPGQSLEE1744 pKa = 4.28CTAEE1748 pKa = 3.66IDD1750 pKa = 4.49IDD1752 pKa = 3.73QDD1754 pKa = 3.56DD1755 pKa = 4.14VNRR1758 pKa = 11.84GEE1760 pKa = 4.22IEE1762 pKa = 4.26LEE1764 pKa = 4.09FEE1766 pKa = 4.35VTADD1770 pKa = 3.55NPAGDD1775 pKa = 4.28EE1776 pKa = 4.33STVTDD1781 pKa = 3.65SSSIEE1786 pKa = 4.0LPAKK1790 pKa = 10.29YY1791 pKa = 9.95RR1792 pKa = 11.84IALSLEE1798 pKa = 4.15GSNTTSSDD1806 pKa = 3.25RR1807 pKa = 11.84DD1808 pKa = 3.64DD1809 pKa = 4.82DD1810 pKa = 4.58GKK1812 pKa = 11.3SSSGDD1817 pKa = 3.16EE1818 pKa = 4.05VTFTLTVANRR1828 pKa = 11.84GSVDD1832 pKa = 3.51LTGVEE1837 pKa = 3.99AQVAGLEE1844 pKa = 4.22GLVCQQFIPSTVGQRR1859 pKa = 11.84RR1860 pKa = 11.84LSWAEE1865 pKa = 3.81TNTDD1869 pKa = 2.91QDD1871 pKa = 3.23IFPPDD1876 pKa = 3.31EE1877 pKa = 5.03EE1878 pKa = 4.78YY1879 pKa = 10.59TCTASHH1885 pKa = 7.55ILTQDD1890 pKa = 3.38DD1891 pKa = 3.58VDD1893 pKa = 4.51AGTLYY1898 pKa = 9.37RR1899 pKa = 11.84TASVSSQARR1908 pKa = 11.84DD1909 pKa = 3.41PTGTLVVAGAEE1920 pKa = 4.06EE1921 pKa = 4.2TLALVAYY1928 pKa = 7.25PAIVVVIVSGHH1939 pKa = 4.72AA1940 pKa = 3.0

Molecular weight: 200.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8LM76|D8LM76_ECTSI Uncharacterized protein OS=Ectocarpus siliculosus OX=2880 GN=Esi_0392_0004 PE=4 SV=1
MM1 pKa = 7.57AEE3 pKa = 3.94SRR5 pKa = 11.84RR6 pKa = 11.84TIATTTTKK14 pKa = 10.19RR15 pKa = 11.84GTMATSWMTTKK26 pKa = 10.52RR27 pKa = 11.84RR28 pKa = 11.84TTARR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84ATPTRR39 pKa = 11.84TTTTTRR45 pKa = 11.84RR46 pKa = 11.84SRR48 pKa = 11.84SKK50 pKa = 10.97AFRR53 pKa = 11.84TTVSPRR59 pKa = 11.84SKK61 pKa = 10.77SS62 pKa = 3.3

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16334

0

16334

8478560

22

17422

519.1

55.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.427 ± 0.035

1.558 ± 0.01

5.673 ± 0.019

6.842 ± 0.023

2.906 ± 0.013

10.541 ± 0.036

1.98 ± 0.008

2.893 ± 0.014

4.139 ± 0.021

8.126 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.01

2.688 ± 0.01

5.423 ± 0.025

3.29 ± 0.013

7.156 ± 0.028

7.975 ± 0.024

5.315 ± 0.018

6.882 ± 0.023

1.229 ± 0.007

1.821 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski