Amino acid dipepetide frequency for Flavobacteriales bacterium ALC-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.734AlaAla: 3.734 ± 0.076	0.524AlaCys: 0.524 ± 0.026	3.246AlaAsp: 3.246 ± 0.057	3.816AlaGlu: 3.816 ± 0.07	3.243AlaPhe: 3.243 ± 0.061	3.782AlaGly: 3.782 ± 0.07	1.027AlaHis: 1.027 ± 0.032	5.633AlaIle: 5.633 ± 0.079	4.752AlaLys: 4.752 ± 0.078	6.244AlaLeu: 6.244 ± 0.085	1.466AlaMet: 1.466 ± 0.041	3.643AlaAsn: 3.643 ± 0.063	1.653AlaPro: 1.653 ± 0.041	2.2AlaGln: 2.2 ± 0.039	1.824AlaArg: 1.824 ± 0.045	4.2AlaSer: 4.2 ± 0.072	3.573AlaThr: 3.573 ± 0.089	3.684AlaVal: 3.684 ± 0.066	0.591AlaTrp: 0.591 ± 0.026	2.541AlaTyr: 2.541 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.421CysAla: 0.421 ± 0.02	0.104CysCys: 0.104 ± 0.01	0.541CysAsp: 0.541 ± 0.031	0.477CysGlu: 0.477 ± 0.024	0.45CysPhe: 0.45 ± 0.02	0.582CysGly: 0.582 ± 0.027	0.158CysHis: 0.158 ± 0.011	0.601CysIle: 0.601 ± 0.02	0.486CysLys: 0.486 ± 0.024	0.627CysLeu: 0.627 ± 0.022	0.136CysMet: 0.136 ± 0.01	0.478CysAsn: 0.478 ± 0.022	0.306CysPro: 0.306 ± 0.022	0.191CysGln: 0.191 ± 0.014	0.163CysArg: 0.163 ± 0.012	0.515CysSer: 0.515 ± 0.028	0.398CysThr: 0.398 ± 0.02	0.427CysVal: 0.427 ± 0.023	0.069CysTrp: 0.069 ± 0.01	0.318CysTyr: 0.318 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.016AspAla: 4.016 ± 0.073	0.453AspCys: 0.453 ± 0.021	3.62AspAsp: 3.62 ± 0.071	3.809AspGlu: 3.809 ± 0.07	3.756AspPhe: 3.756 ± 0.055	4.076AspGly: 4.076 ± 0.103	0.808AspHis: 0.808 ± 0.027	5.133AspIle: 5.133 ± 0.077	4.313AspLys: 4.313 ± 0.067	5.461AspLeu: 5.461 ± 0.071	1.255AspMet: 1.255 ± 0.031	3.781AspAsn: 3.781 ± 0.071	1.657AspPro: 1.657 ± 0.049	1.376AspGln: 1.376 ± 0.035	1.747AspArg: 1.747 ± 0.044	3.644AspSer: 3.644 ± 0.064	3.088AspThr: 3.088 ± 0.069	3.869AspVal: 3.869 ± 0.067	0.723AspTrp: 0.723 ± 0.025	3.182AspTyr: 3.182 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.403GluAla: 4.403 ± 0.083	0.344GluCys: 0.344 ± 0.02	4.102GluAsp: 4.102 ± 0.061	4.2GluGlu: 4.2 ± 0.081	3.041GluPhe: 3.041 ± 0.055	3.626GluGly: 3.626 ± 0.061	1.113GluHis: 1.113 ± 0.033	5.174GluIle: 5.174 ± 0.082	5.133GluLys: 5.133 ± 0.087	6.081GluLeu: 6.081 ± 0.082	1.38GluMet: 1.38 ± 0.037	4.623GluAsn: 4.623 ± 0.073	1.569GluPro: 1.569 ± 0.038	2.266GluGln: 2.266 ± 0.048	2.354GluArg: 2.354 ± 0.05	3.544GluSer: 3.544 ± 0.062	3.91GluThr: 3.91 ± 0.064	4.106GluVal: 4.106 ± 0.056	0.584GluTrp: 0.584 ± 0.023	2.185GluTyr: 2.185 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.878PheAla: 2.878 ± 0.059	0.416PheCys: 0.416 ± 0.018	3.358PheAsp: 3.358 ± 0.06	3.402PheGlu: 3.402 ± 0.058	2.662PhePhe: 2.662 ± 0.06	3.713PheGly: 3.713 ± 0.071	0.808PheHis: 0.808 ± 0.028	4.126PheIle: 4.126 ± 0.078	4.115PheLys: 4.115 ± 0.075	4.369PheLeu: 4.369 ± 0.069	1.079PheMet: 1.079 ± 0.034	3.741PheAsn: 3.741 ± 0.06	1.653PhePro: 1.653 ± 0.044	1.443PheGln: 1.443 ± 0.037	1.495PheArg: 1.495 ± 0.041	4.068PheSer: 4.068 ± 0.077	3.072PheThr: 3.072 ± 0.063	2.991PheVal: 2.991 ± 0.047	0.562PheTrp: 0.562 ± 0.023	2.192PheTyr: 2.192 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	3.842GlyAla: 3.842 ± 0.073	0.614GlyCys: 0.614 ± 0.034	3.688GlyAsp: 3.688 ± 0.08	3.566GlyGlu: 3.566 ± 0.067	3.688GlyPhe: 3.688 ± 0.065	4.469GlyGly: 4.469 ± 0.092	1.065GlyHis: 1.065 ± 0.032	5.202GlyIle: 5.202 ± 0.08	4.704GlyLys: 4.704 ± 0.078	5.667GlyLeu: 5.667 ± 0.086	1.438GlyMet: 1.438 ± 0.038	3.901GlyAsn: 3.901 ± 0.079	1.335GlyPro: 1.335 ± 0.036	1.8GlyGln: 1.8 ± 0.043	1.983GlyArg: 1.983 ± 0.045	3.981GlySer: 3.981 ± 0.082	4.003GlyThr: 4.003 ± 0.1	4.16GlyVal: 4.16 ± 0.057	0.738GlyTrp: 0.738 ± 0.027	2.813GlyTyr: 2.813 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.862HisAla: 0.862 ± 0.029	0.164HisCys: 0.164 ± 0.012	0.826HisAsp: 0.826 ± 0.024	0.839HisGlu: 0.839 ± 0.026	1.08HisPhe: 1.08 ± 0.033	0.943HisGly: 0.943 ± 0.03	0.46HisHis: 0.46 ± 0.024	1.443HisIle: 1.443 ± 0.035	1.242HisLys: 1.242 ± 0.037	1.752HisLeu: 1.752 ± 0.041	0.318HisMet: 0.318 ± 0.015	1.068HisAsn: 1.068 ± 0.031	0.789HisPro: 0.789 ± 0.025	0.656HisGln: 0.656 ± 0.025	0.603HisArg: 0.603 ± 0.025	1.067HisSer: 1.067 ± 0.035	0.902HisThr: 0.902 ± 0.026	0.878HisVal: 0.878 ± 0.025	0.207HisTrp: 0.207 ± 0.013	0.837HisTyr: 0.837 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.594IleAla: 5.594 ± 0.077	0.616IleCys: 0.616 ± 0.024	5.497IleAsp: 5.497 ± 0.075	6.037IleGlu: 6.037 ± 0.078	3.643IlePhe: 3.643 ± 0.062	5.237IleGly: 5.237 ± 0.083	1.241IleHis: 1.241 ± 0.038	6.697IleIle: 6.697 ± 0.106	6.224IleLys: 6.224 ± 0.088	7.102IleLeu: 7.102 ± 0.101	1.421IleMet: 1.421 ± 0.034	5.268IleAsn: 5.268 ± 0.091	3.093IlePro: 3.093 ± 0.062	2.312IleGln: 2.312 ± 0.048	2.406IleArg: 2.406 ± 0.051	6.052IleSer: 6.052 ± 0.093	5.141IleThr: 5.141 ± 0.083	5.035IleVal: 5.035 ± 0.073	0.736IleTrp: 0.736 ± 0.028	3.044IleTyr: 3.044 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.263LysAla: 5.263 ± 0.084	0.322LysCys: 0.322 ± 0.018	4.893LysAsp: 4.893 ± 0.082	5.572LysGlu: 5.572 ± 0.093	2.906LysPhe: 2.906 ± 0.054	4.42LysGly: 4.42 ± 0.07	1.563LysHis: 1.563 ± 0.039	5.951LysIle: 5.951 ± 0.084	6.505LysLys: 6.505 ± 0.1	6.756LysLeu: 6.756 ± 0.091	1.818LysMet: 1.818 ± 0.047	5.056LysAsn: 5.056 ± 0.075	2.472LysPro: 2.472 ± 0.056	2.945LysGln: 2.945 ± 0.062	3.053LysArg: 3.053 ± 0.053	5.164LysSer: 5.164 ± 0.081	5.031LysThr: 5.031 ± 0.079	4.689LysVal: 4.689 ± 0.075	0.771LysTrp: 0.771 ± 0.028	3.023LysTyr: 3.023 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	5.286LeuAla: 5.286 ± 0.074	0.688LeuCys: 0.688 ± 0.025	5.39LeuAsp: 5.39 ± 0.082	5.879LeuGlu: 5.879 ± 0.084	4.899LeuPhe: 4.899 ± 0.091	5.612LeuGly: 5.612 ± 0.08	1.445LeuHis: 1.445 ± 0.036	7.269LeuIle: 7.269 ± 0.091	8.117LeuLys: 8.117 ± 0.104	8.42LeuLeu: 8.42 ± 0.118	1.971LeuMet: 1.971 ± 0.048	6.231LeuAsn: 6.231 ± 0.082	3.337LeuPro: 3.337 ± 0.054	2.964LeuGln: 2.964 ± 0.05	3.001LeuArg: 3.001 ± 0.058	6.804LeuSer: 6.804 ± 0.089	4.996LeuThr: 4.996 ± 0.061	5.392LeuVal: 5.392 ± 0.07	0.832LeuTrp: 0.832 ± 0.032	3.257LeuTyr: 3.257 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	1.477MetAla: 1.477 ± 0.043	0.144MetCys: 0.144 ± 0.012	1.091MetAsp: 1.091 ± 0.035	1.129MetGlu: 1.129 ± 0.036	0.897MetPhe: 0.897 ± 0.034	1.18MetGly: 1.18 ± 0.036	0.411MetHis: 0.411 ± 0.019	1.533MetIle: 1.533 ± 0.042	2.065MetLys: 2.065 ± 0.041	1.941MetLeu: 1.941 ± 0.04	0.523MetMet: 0.523 ± 0.023	1.267MetAsn: 1.267 ± 0.033	0.844MetPro: 0.844 ± 0.027	0.826MetGln: 0.826 ± 0.026	0.811MetArg: 0.811 ± 0.029	1.508MetSer: 1.508 ± 0.036	1.165MetThr: 1.165 ± 0.032	1.278MetVal: 1.278 ± 0.037	0.14MetTrp: 0.14 ± 0.011	0.704MetTyr: 0.704 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	4.005AsnAla: 4.005 ± 0.07	0.533AsnCys: 0.533 ± 0.023	3.939AsnAsp: 3.939 ± 0.074	3.9AsnGlu: 3.9 ± 0.059	3.249AsnPhe: 3.249 ± 0.062	4.39AsnGly: 4.39 ± 0.091	1.071AsnHis: 1.071 ± 0.031	5.187AsnIle: 5.187 ± 0.081	4.635AsnLys: 4.635 ± 0.071	5.59AsnLeu: 5.59 ± 0.071	1.353AsnMet: 1.353 ± 0.033	4.479AsnAsn: 4.479 ± 0.095	2.861AsnPro: 2.861 ± 0.057	2.252AsnGln: 2.252 ± 0.049	2.224AsnArg: 2.224 ± 0.048	4.49AsnSer: 4.49 ± 0.079	4.238AsnThr: 4.238 ± 0.079	3.801AsnVal: 3.801 ± 0.065	0.817AsnTrp: 0.817 ± 0.032	3.106AsnTyr: 3.106 ± 0.065	0.0AsnXaa: 0.0 ± 0.0
Pro	1.628ProAla: 1.628 ± 0.045	0.228ProCys: 0.228 ± 0.016	1.976ProAsp: 1.976 ± 0.048	2.619ProGlu: 2.619 ± 0.044	1.803ProPhe: 1.803 ± 0.043	1.714ProGly: 1.714 ± 0.046	0.562ProHis: 0.562 ± 0.022	2.79ProIle: 2.79 ± 0.052	2.587ProLys: 2.587 ± 0.052	2.834ProLeu: 2.834 ± 0.057	0.67ProMet: 0.67 ± 0.025	2.451ProAsn: 2.451 ± 0.054	0.784ProPro: 0.784 ± 0.043	1.006ProGln: 1.006 ± 0.035	0.818ProArg: 0.818 ± 0.025	2.129ProSer: 2.129 ± 0.046	1.989ProThr: 1.989 ± 0.056	1.925ProVal: 1.925 ± 0.04	0.327ProTrp: 0.327 ± 0.019	1.319ProTyr: 1.319 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	1.825GlnAla: 1.825 ± 0.047	0.186GlnCys: 0.186 ± 0.012	1.73GlnAsp: 1.73 ± 0.04	1.948GlnGlu: 1.948 ± 0.048	1.719GlnPhe: 1.719 ± 0.038	1.652GlnGly: 1.652 ± 0.039	0.552GlnHis: 0.552 ± 0.024	2.586GlnIle: 2.586 ± 0.046	2.54GlnLys: 2.54 ± 0.052	3.505GlnLeu: 3.505 ± 0.065	0.737GlnMet: 0.737 ± 0.022	2.104GlnAsn: 2.104 ± 0.05	1.117GlnPro: 1.117 ± 0.031	1.227GlnGln: 1.227 ± 0.038	1.176GlnArg: 1.176 ± 0.031	1.942GlnSer: 1.942 ± 0.044	1.841GlnThr: 1.841 ± 0.037	1.822GlnVal: 1.822 ± 0.038	0.349GlnTrp: 0.349 ± 0.019	1.293GlnTyr: 1.293 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.052ArgAla: 2.052 ± 0.039	0.168ArgCys: 0.168 ± 0.011	1.756ArgAsp: 1.756 ± 0.044	1.921ArgGlu: 1.921 ± 0.043	1.767ArgPhe: 1.767 ± 0.039	1.867ArgGly: 1.867 ± 0.046	0.606ArgHis: 0.606 ± 0.021	2.759ArgIle: 2.759 ± 0.05	2.471ArgLys: 2.471 ± 0.052	3.254ArgLeu: 3.254 ± 0.06	0.748ArgMet: 0.748 ± 0.025	1.99ArgAsn: 1.99 ± 0.045	0.973ArgPro: 0.973 ± 0.03	1.129ArgGln: 1.129 ± 0.034	1.305ArgArg: 1.305 ± 0.039	1.854ArgSer: 1.854 ± 0.051	1.801ArgThr: 1.801 ± 0.041	2.145ArgVal: 2.145 ± 0.048	0.363ArgTrp: 0.363 ± 0.02	1.545ArgTyr: 1.545 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.744SerAla: 3.744 ± 0.061	0.656SerCys: 0.656 ± 0.025	3.876SerAsp: 3.876 ± 0.067	4.219SerGlu: 4.219 ± 0.059	3.769SerPhe: 3.769 ± 0.064	4.705SerGly: 4.705 ± 0.089	1.127SerHis: 1.127 ± 0.036	5.896SerIle: 5.896 ± 0.09	5.402SerLys: 5.402 ± 0.074	6.085SerLeu: 6.085 ± 0.075	1.329SerMet: 1.329 ± 0.033	4.582SerAsn: 4.582 ± 0.073	2.011SerPro: 2.011 ± 0.047	2.168SerGln: 2.168 ± 0.048	2.017SerArg: 2.017 ± 0.04	4.451SerSer: 4.451 ± 0.076	3.8SerThr: 3.8 ± 0.071	4.068SerVal: 4.068 ± 0.057	0.71SerTrp: 0.71 ± 0.029	2.867SerTyr: 2.867 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	3.602ThrAla: 3.602 ± 0.074	0.362ThrCys: 0.362 ± 0.018	3.479ThrAsp: 3.479 ± 0.078	3.587ThrGlu: 3.587 ± 0.056	3.182ThrPhe: 3.182 ± 0.057	3.863ThrGly: 3.863 ± 0.082	1.006ThrHis: 1.006 ± 0.029	5.557ThrIle: 5.557 ± 0.085	4.17ThrLys: 4.17 ± 0.077	5.452ThrLeu: 5.452 ± 0.062	0.955ThrMet: 0.955 ± 0.031	3.816ThrAsn: 3.816 ± 0.081	2.2ThrPro: 2.2 ± 0.051	1.786ThrGln: 1.786 ± 0.046	1.609ThrArg: 1.609 ± 0.043	4.195ThrSer: 4.195 ± 0.073	3.812ThrThr: 3.812 ± 0.089	3.776ThrVal: 3.776 ± 0.105	0.623ThrTrp: 0.623 ± 0.026	2.598ThrTyr: 2.598 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	3.794ValAla: 3.794 ± 0.062	0.506ValCys: 0.506 ± 0.025	3.66ValAsp: 3.66 ± 0.062	3.95ValGlu: 3.95 ± 0.059	3.37ValPhe: 3.37 ± 0.065	3.68ValGly: 3.68 ± 0.063	0.925ValHis: 0.925 ± 0.027	5.083ValIle: 5.083 ± 0.074	4.508ValLys: 4.508 ± 0.062	5.86ValLeu: 5.86 ± 0.075	1.288ValMet: 1.288 ± 0.037	3.862ValAsn: 3.862 ± 0.067	1.882ValPro: 1.882 ± 0.04	1.485ValGln: 1.485 ± 0.037	1.856ValArg: 1.856 ± 0.041	4.49ValSer: 4.49 ± 0.071	3.743ValThr: 3.743 ± 0.087	4.103ValVal: 4.103 ± 0.075	0.574ValTrp: 0.574 ± 0.021	2.382ValTyr: 2.382 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.557TrpAla: 0.557 ± 0.025	0.087TrpCys: 0.087 ± 0.008	0.592TrpAsp: 0.592 ± 0.021	0.638TrpGlu: 0.638 ± 0.023	0.594TrpPhe: 0.594 ± 0.025	0.569TrpGly: 0.569 ± 0.028	0.2TrpHis: 0.2 ± 0.011	0.746TrpIle: 0.746 ± 0.027	0.775TrpLys: 0.775 ± 0.028	1.011TrpLeu: 1.011 ± 0.029	0.288TrpMet: 0.288 ± 0.017	0.781TrpAsn: 0.781 ± 0.031	0.212TrpPro: 0.212 ± 0.014	0.407TrpGln: 0.407 ± 0.018	0.394TrpArg: 0.394 ± 0.021	0.678TrpSer: 0.678 ± 0.03	0.593TrpThr: 0.593 ± 0.028	0.607TrpVal: 0.607 ± 0.021	0.159TrpTrp: 0.159 ± 0.012	0.442TrpTyr: 0.442 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.392TyrAla: 2.392 ± 0.044	0.334TyrCys: 0.334 ± 0.017	2.508TyrAsp: 2.508 ± 0.048	2.227TyrGlu: 2.227 ± 0.042	2.427TyrPhe: 2.427 ± 0.048	2.577TyrGly: 2.577 ± 0.051	0.79TyrHis: 0.79 ± 0.027	3.029TyrIle: 3.029 ± 0.064	3.43TyrLys: 3.43 ± 0.069	3.781TyrLeu: 3.781 ± 0.067	0.758TyrMet: 0.758 ± 0.024	3.002TyrAsn: 3.002 ± 0.06	1.41TyrPro: 1.41 ± 0.036	1.409TyrGln: 1.409 ± 0.042	1.636TyrArg: 1.636 ± 0.04	2.706TyrSer: 2.706 ± 0.046	2.534TyrThr: 2.534 ± 0.057	2.244TyrVal: 2.244 ± 0.045	0.454TyrTrp: 0.454 ± 0.02	1.953TyrTyr: 1.953 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3427 proteins (1172252 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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