Amino acid dipepetide frequency for Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.322AlaAla: 13.322 ± 0.13	0.963AlaCys: 0.963 ± 0.029	5.986AlaAsp: 5.986 ± 0.073	6.558AlaGlu: 6.558 ± 0.082	3.395AlaPhe: 3.395 ± 0.056	7.997AlaGly: 7.997 ± 0.088	2.007AlaHis: 2.007 ± 0.048	5.167AlaIle: 5.167 ± 0.066	2.601AlaLys: 2.601 ± 0.052	12.24AlaLeu: 12.24 ± 0.146	2.089AlaMet: 2.089 ± 0.039	2.892AlaAsn: 2.892 ± 0.065	5.76AlaPro: 5.76 ± 0.085	3.928AlaGln: 3.928 ± 0.063	9.762AlaArg: 9.762 ± 0.114	6.353AlaSer: 6.353 ± 0.072	6.055AlaThr: 6.055 ± 0.072	8.049AlaVal: 8.049 ± 0.083	1.995AlaTrp: 1.995 ± 0.039	1.722AlaTyr: 1.722 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.672CysAla: 0.672 ± 0.022	0.13CysCys: 0.13 ± 0.009	0.612CysAsp: 0.612 ± 0.023	0.443CysGlu: 0.443 ± 0.019	0.282CysPhe: 0.282 ± 0.013	0.817CysGly: 0.817 ± 0.028	0.37CysHis: 0.37 ± 0.021	0.183CysIle: 0.183 ± 0.014	0.176CysLys: 0.176 ± 0.012	1.031CysLeu: 1.031 ± 0.026	0.108CysMet: 0.108 ± 0.008	0.192CysAsn: 0.192 ± 0.012	0.628CysPro: 0.628 ± 0.023	0.305CysGln: 0.305 ± 0.015	0.835CysArg: 0.835 ± 0.026	0.416CysSer: 0.416 ± 0.017	0.263CysThr: 0.263 ± 0.016	0.639CysVal: 0.639 ± 0.025	0.175CysTrp: 0.175 ± 0.009	0.227CysTyr: 0.227 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.761AspAla: 5.761 ± 0.068	0.423AspCys: 0.423 ± 0.019	3.637AspAsp: 3.637 ± 0.08	3.306AspGlu: 3.306 ± 0.046	1.976AspPhe: 1.976 ± 0.041	4.65AspGly: 4.65 ± 0.09	1.879AspHis: 1.879 ± 0.043	1.387AspIle: 1.387 ± 0.031	0.998AspLys: 0.998 ± 0.031	7.629AspLeu: 7.629 ± 0.085	0.648AspMet: 0.648 ± 0.022	1.16AspAsn: 1.16 ± 0.036	6.177AspPro: 6.177 ± 0.082	2.873AspGln: 2.873 ± 0.043	5.384AspArg: 5.384 ± 0.072	2.898AspSer: 2.898 ± 0.054	2.098AspThr: 2.098 ± 0.061	2.939AspVal: 2.939 ± 0.043	1.144AspTrp: 1.144 ± 0.032	1.13AspTyr: 1.13 ± 0.028	0.0AspXaa: 0.0 ± 0.0
Glu	7.319GluAla: 7.319 ± 0.1	0.41GluCys: 0.41 ± 0.019	2.493GluAsp: 2.493 ± 0.044	3.523GluGlu: 3.523 ± 0.072	2.127GluPhe: 2.127 ± 0.038	4.105GluGly: 4.105 ± 0.054	1.028GluHis: 1.028 ± 0.029	2.655GluIle: 2.655 ± 0.047	1.322GluLys: 1.322 ± 0.041	6.236GluLeu: 6.236 ± 0.078	1.014GluMet: 1.014 ± 0.029	1.269GluAsn: 1.269 ± 0.031	4.047GluPro: 4.047 ± 0.065	1.997GluGln: 1.997 ± 0.045	5.653GluArg: 5.653 ± 0.077	3.495GluSer: 3.495 ± 0.054	3.92GluThr: 3.92 ± 0.056	4.2GluVal: 4.2 ± 0.06	0.936GluTrp: 0.936 ± 0.031	0.943GluTyr: 0.943 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.413PheAla: 3.413 ± 0.055	0.295PheCys: 0.295 ± 0.013	2.668PheAsp: 2.668 ± 0.051	2.323PheGlu: 2.323 ± 0.041	1.095PhePhe: 1.095 ± 0.034	3.102PheGly: 3.102 ± 0.051	0.839PheHis: 0.839 ± 0.027	1.057PheIle: 1.057 ± 0.03	0.816PheLys: 0.816 ± 0.023	3.586PheLeu: 3.586 ± 0.06	0.439PheMet: 0.439 ± 0.018	1.168PheAsn: 1.168 ± 0.037	1.781PhePro: 1.781 ± 0.042	1.366PheGln: 1.366 ± 0.032	2.48PheArg: 2.48 ± 0.044	1.602PheSer: 1.602 ± 0.037	1.753PheThr: 1.753 ± 0.039	2.425PheVal: 2.425 ± 0.041	0.552PheTrp: 0.552 ± 0.023	0.652PheTyr: 0.652 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	6.082GlyAla: 6.082 ± 0.082	0.883GlyCys: 0.883 ± 0.027	4.439GlyAsp: 4.439 ± 0.09	4.117GlyGlu: 4.117 ± 0.053	3.29GlyPhe: 3.29 ± 0.057	7.23GlyGly: 7.23 ± 0.138	1.672GlyHis: 1.672 ± 0.038	3.269GlyIle: 3.269 ± 0.055	2.123GlyLys: 2.123 ± 0.052	9.428GlyLeu: 9.428 ± 0.085	1.76GlyMet: 1.76 ± 0.046	2.087GlyAsn: 2.087 ± 0.117	4.701GlyPro: 4.701 ± 0.054	2.948GlyGln: 2.948 ± 0.065	7.279GlyArg: 7.279 ± 0.08	5.064GlySer: 5.064 ± 0.083	4.182GlyThr: 4.182 ± 0.081	6.397GlyVal: 6.397 ± 0.094	1.824GlyTrp: 1.824 ± 0.041	1.691GlyTyr: 1.691 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.508HisAla: 2.508 ± 0.056	0.247HisCys: 0.247 ± 0.014	1.372HisAsp: 1.372 ± 0.031	1.072HisGlu: 1.072 ± 0.027	0.658HisPhe: 0.658 ± 0.022	1.753HisGly: 1.753 ± 0.043	0.867HisHis: 0.867 ± 0.026	0.559HisIle: 0.559 ± 0.021	0.379HisLys: 0.379 ± 0.02	2.618HisLeu: 2.618 ± 0.048	0.219HisMet: 0.219 ± 0.012	0.51HisAsn: 0.51 ± 0.019	2.228HisPro: 2.228 ± 0.042	0.854HisGln: 0.854 ± 0.026	1.988HisArg: 1.988 ± 0.045	1.0HisSer: 1.0 ± 0.031	0.856HisThr: 0.856 ± 0.026	1.305HisVal: 1.305 ± 0.03	0.412HisTrp: 0.412 ± 0.015	0.449HisTyr: 0.449 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.0IleAla: 5.0 ± 0.067	0.378IleCys: 0.378 ± 0.018	3.382IleAsp: 3.382 ± 0.057	3.236IleGlu: 3.236 ± 0.053	1.131IlePhe: 1.131 ± 0.036	3.432IleGly: 3.432 ± 0.071	1.129IleHis: 1.129 ± 0.028	1.376IleIle: 1.376 ± 0.029	1.138IleLys: 1.138 ± 0.029	4.587IleLeu: 4.587 ± 0.064	0.506IleMet: 0.506 ± 0.019	1.336IleAsn: 1.336 ± 0.034	2.77IlePro: 2.77 ± 0.047	1.68IleGln: 1.68 ± 0.037	3.632IleArg: 3.632 ± 0.052	1.863IleSer: 1.863 ± 0.043	2.009IleThr: 2.009 ± 0.041	3.344IleVal: 3.344 ± 0.045	0.552IleTrp: 0.552 ± 0.018	0.834IleTyr: 0.834 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	2.769LysAla: 2.769 ± 0.048	0.158LysCys: 0.158 ± 0.011	1.034LysAsp: 1.034 ± 0.027	1.302LysGlu: 1.302 ± 0.033	0.699LysPhe: 0.699 ± 0.023	1.766LysGly: 1.766 ± 0.043	0.441LysHis: 0.441 ± 0.02	1.115LysIle: 1.115 ± 0.03	0.727LysLys: 0.727 ± 0.03	2.729LysLeu: 2.729 ± 0.053	0.439LysMet: 0.439 ± 0.018	0.583LysAsn: 0.583 ± 0.021	1.997LysPro: 1.997 ± 0.043	0.722LysGln: 0.722 ± 0.026	2.136LysArg: 2.136 ± 0.043	1.239LysSer: 1.239 ± 0.031	1.682LysThr: 1.682 ± 0.041	2.012LysVal: 2.012 ± 0.053	0.239LysTrp: 0.239 ± 0.013	0.488LysTyr: 0.488 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.029LeuAla: 14.029 ± 0.143	0.836LeuCys: 0.836 ± 0.023	7.463LeuAsp: 7.463 ± 0.077	7.374LeuGlu: 7.374 ± 0.087	3.209LeuPhe: 3.209 ± 0.055	9.452LeuGly: 9.452 ± 0.085	1.878LeuHis: 1.878 ± 0.04	5.472LeuIle: 5.472 ± 0.078	3.598LeuLys: 3.598 ± 0.063	9.509LeuLeu: 9.509 ± 0.097	1.899LeuMet: 1.899 ± 0.036	3.32LeuAsn: 3.32 ± 0.052	6.352LeuPro: 6.352 ± 0.076	2.812LeuGln: 2.812 ± 0.046	7.783LeuArg: 7.783 ± 0.092	5.372LeuSer: 5.372 ± 0.067	6.752LeuThr: 6.752 ± 0.073	8.287LeuVal: 8.287 ± 0.085	1.345LeuTrp: 1.345 ± 0.036	1.685LeuTyr: 1.685 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	1.859MetAla: 1.859 ± 0.034	0.111MetCys: 0.111 ± 0.007	0.725MetAsp: 0.725 ± 0.02	0.846MetGlu: 0.846 ± 0.025	0.523MetPhe: 0.523 ± 0.018	1.389MetGly: 1.389 ± 0.046	0.211MetHis: 0.211 ± 0.013	1.078MetIle: 1.078 ± 0.028	0.485MetLys: 0.485 ± 0.015	1.609MetLeu: 1.609 ± 0.035	0.469MetMet: 0.469 ± 0.023	0.623MetAsn: 0.623 ± 0.022	1.029MetPro: 1.029 ± 0.026	0.303MetGln: 0.303 ± 0.016	1.152MetArg: 1.152 ± 0.027	1.314MetSer: 1.314 ± 0.033	1.361MetThr: 1.361 ± 0.029	1.399MetVal: 1.399 ± 0.034	0.163MetTrp: 0.163 ± 0.01	0.21MetTyr: 0.21 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.981AsnAla: 2.981 ± 0.055	0.214AsnCys: 0.214 ± 0.013	1.828AsnAsp: 1.828 ± 0.07	1.346AsnGlu: 1.346 ± 0.027	0.816AsnPhe: 0.816 ± 0.032	2.283AsnGly: 2.283 ± 0.062	0.752AsnHis: 0.752 ± 0.023	0.805AsnIle: 0.805 ± 0.025	0.438AsnLys: 0.438 ± 0.019	3.266AsnLeu: 3.266 ± 0.069	0.338AsnMet: 0.338 ± 0.016	0.823AsnAsn: 0.823 ± 0.047	2.903AsnPro: 2.903 ± 0.056	1.329AsnGln: 1.329 ± 0.035	2.517AsnArg: 2.517 ± 0.05	1.291AsnSer: 1.291 ± 0.036	1.349AsnThr: 1.349 ± 0.056	1.629AsnVal: 1.629 ± 0.052	0.366AsnTrp: 0.366 ± 0.016	0.556AsnTyr: 0.556 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.269ProAla: 7.269 ± 0.102	0.479ProCys: 0.479 ± 0.018	4.588ProAsp: 4.588 ± 0.065	4.672ProGlu: 4.672 ± 0.056	2.13ProPhe: 2.13 ± 0.039	5.593ProGly: 5.593 ± 0.081	1.571ProHis: 1.571 ± 0.03	3.269ProIle: 3.269 ± 0.048	1.605ProLys: 1.605 ± 0.047	6.415ProLeu: 6.415 ± 0.081	1.074ProMet: 1.074 ± 0.027	2.459ProAsn: 2.459 ± 0.046	6.477ProPro: 6.477 ± 0.124	2.179ProGln: 2.179 ± 0.044	5.247ProArg: 5.247 ± 0.067	4.883ProSer: 4.883 ± 0.065	4.645ProThr: 4.645 ± 0.08	4.393ProVal: 4.393 ± 0.063	1.031ProTrp: 1.031 ± 0.029	1.112ProTyr: 1.112 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.759GlnAla: 4.759 ± 0.069	0.236GlnCys: 0.236 ± 0.013	1.347GlnAsp: 1.347 ± 0.031	1.526GlnGlu: 1.526 ± 0.04	1.085GlnPhe: 1.085 ± 0.026	3.06GlnGly: 3.06 ± 0.056	0.545GlnHis: 0.545 ± 0.02	1.559GlnIle: 1.559 ± 0.038	0.662GlnLys: 0.662 ± 0.026	3.305GlnLeu: 3.305 ± 0.057	0.494GlnMet: 0.494 ± 0.018	0.721GlnAsn: 0.721 ± 0.022	3.005GlnPro: 3.005 ± 0.053	0.911GlnGln: 0.911 ± 0.041	3.238GlnArg: 3.238 ± 0.053	1.726GlnSer: 1.726 ± 0.037	2.443GlnThr: 2.443 ± 0.039	2.661GlnVal: 2.661 ± 0.041	0.456GlnTrp: 0.456 ± 0.017	0.46GlnTyr: 0.46 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.04ArgAla: 8.04 ± 0.095	0.735ArgCys: 0.735 ± 0.023	5.209ArgAsp: 5.209 ± 0.059	4.492ArgGlu: 4.492 ± 0.063	3.638ArgPhe: 3.638 ± 0.05	5.778ArgGly: 5.778 ± 0.07	2.017ArgHis: 2.017 ± 0.042	4.128ArgIle: 4.128 ± 0.058	1.856ArgLys: 1.856 ± 0.043	10.686ArgLeu: 10.686 ± 0.114	1.523ArgMet: 1.523 ± 0.033	1.967ArgAsn: 1.967 ± 0.046	5.706ArgPro: 5.706 ± 0.087	2.916ArgGln: 2.916 ± 0.051	8.1ArgArg: 8.1 ± 0.109	4.601ArgSer: 4.601 ± 0.061	3.567ArgThr: 3.567 ± 0.052	6.304ArgVal: 6.304 ± 0.068	1.583ArgTrp: 1.583 ± 0.037	1.687ArgTyr: 1.687 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	5.405SerAla: 5.405 ± 0.062	0.509SerCys: 0.509 ± 0.022	3.169SerAsp: 3.169 ± 0.044	2.878SerGlu: 2.878 ± 0.052	1.823SerPhe: 1.823 ± 0.034	4.901SerGly: 4.901 ± 0.097	1.314SerHis: 1.314 ± 0.027	2.241SerIle: 2.241 ± 0.045	1.401SerLys: 1.401 ± 0.035	5.584SerLeu: 5.584 ± 0.064	1.032SerMet: 1.032 ± 0.028	1.861SerAsn: 1.861 ± 0.045	4.615SerPro: 4.615 ± 0.068	2.097SerGln: 2.097 ± 0.038	4.458SerArg: 4.458 ± 0.055	4.504SerSer: 4.504 ± 0.093	3.122SerThr: 3.122 ± 0.051	3.426SerVal: 3.426 ± 0.056	1.168SerTrp: 1.168 ± 0.032	0.956SerTyr: 0.956 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	5.779ThrAla: 5.779 ± 0.068	0.401ThrCys: 0.401 ± 0.018	2.601ThrAsp: 2.601 ± 0.042	2.343ThrGlu: 2.343 ± 0.043	1.809ThrPhe: 1.809 ± 0.041	4.198ThrGly: 4.198 ± 0.064	1.205ThrHis: 1.205 ± 0.028	3.109ThrIle: 3.109 ± 0.062	1.147ThrLys: 1.147 ± 0.03	6.981ThrLeu: 6.981 ± 0.084	0.891ThrMet: 0.891 ± 0.024	1.619ThrAsn: 1.619 ± 0.041	4.904ThrPro: 4.904 ± 0.073	1.764ThrGln: 1.764 ± 0.038	4.371ThrArg: 4.371 ± 0.055	3.051ThrSer: 3.051 ± 0.047	3.633ThrThr: 3.633 ± 0.07	3.822ThrVal: 3.822 ± 0.074	0.871ThrTrp: 0.871 ± 0.025	1.048ThrTyr: 1.048 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	8.737ValAla: 8.737 ± 0.087	0.72ValCys: 0.72 ± 0.026	3.407ValAsp: 3.407 ± 0.045	5.386ValGlu: 5.386 ± 0.069	2.427ValPhe: 2.427 ± 0.046	6.35ValGly: 6.35 ± 0.078	1.33ValHis: 1.33 ± 0.031	3.396ValIle: 3.396 ± 0.048	1.957ValLys: 1.957 ± 0.042	6.926ValLeu: 6.926 ± 0.078	1.292ValMet: 1.292 ± 0.031	2.019ValAsn: 2.019 ± 0.049	4.129ValPro: 4.129 ± 0.07	2.028ValGln: 2.028 ± 0.038	5.189ValArg: 5.189 ± 0.06	3.802ValSer: 3.802 ± 0.059	3.982ValThr: 3.982 ± 0.076	7.17ValVal: 7.17 ± 0.086	1.323ValTrp: 1.323 ± 0.037	1.278ValTyr: 1.278 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.375TrpAla: 1.375 ± 0.033	0.177TrpCys: 0.177 ± 0.011	0.829TrpAsp: 0.829 ± 0.026	0.822TrpGlu: 0.822 ± 0.026	0.681TrpPhe: 0.681 ± 0.024	1.1TrpGly: 1.1 ± 0.031	0.298TrpHis: 0.298 ± 0.014	0.978TrpIle: 0.978 ± 0.027	0.426TrpLys: 0.426 ± 0.016	2.05TrpLeu: 2.05 ± 0.043	0.385TrpMet: 0.385 ± 0.018	0.685TrpAsn: 0.685 ± 0.023	0.886TrpPro: 0.886 ± 0.027	0.403TrpGln: 0.403 ± 0.017	1.553TrpArg: 1.553 ± 0.04	1.193TrpSer: 1.193 ± 0.035	1.09TrpThr: 1.09 ± 0.029	1.198TrpVal: 1.198 ± 0.033	0.39TrpTrp: 0.39 ± 0.015	0.296TrpTyr: 0.296 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.764TyrAla: 1.764 ± 0.04	0.199TyrCys: 0.199 ± 0.011	1.31TyrAsp: 1.31 ± 0.038	1.125TyrGlu: 1.125 ± 0.032	0.618TyrPhe: 0.618 ± 0.022	1.511TyrGly: 1.511 ± 0.035	0.503TyrHis: 0.503 ± 0.02	0.514TyrIle: 0.514 ± 0.018	0.422TyrLys: 0.422 ± 0.02	2.012TyrLeu: 2.012 ± 0.042	0.239TyrMet: 0.239 ± 0.013	0.535TyrAsn: 0.535 ± 0.023	0.996TyrPro: 0.996 ± 0.032	0.784TyrGln: 0.784 ± 0.023	1.777TyrArg: 1.777 ± 0.036	0.798TyrSer: 0.798 ± 0.023	0.786TyrThr: 0.786 ± 0.03	1.247TyrVal: 1.247 ± 0.032	0.287TyrTrp: 0.287 ± 0.016	0.5TyrTyr: 0.5 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3721 proteins (1549566 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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