Candidatus Synechococcus spongiarum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165B3F4|A0A165B3F4_9SYNE Polyribonucleotide nucleotidyltransferase OS=Candidatus Synechococcus spongiarum OX=431041 GN=pnp PE=3 SV=1
DDD2 pKa = 4.68RR3 pKa = 11.84WQSNNIYYY11 pKa = 9.99DDD13 pKa = 4.08DD14 pKa = 3.87SAMPMGNYYY23 pKa = 9.73YY24 pKa = 9.08TGPDDD29 pKa = 3.31NEEE32 pKa = 3.67KKK34 pKa = 9.85EEE36 pKa = 4.43YY37 pKa = 9.92FGYYY41 pKa = 10.25LVDDD45 pKa = 3.55DDD47 pKa = 3.69TINLHHH53 pKa = 6.34HH54 pKa = 6.93SIPYYY59 pKa = 10.03LE

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165B0H1|A0A165B0H1_9SYNE Molybdopterin molybdenumtransferase (Fragment) OS=Candidatus Synechococcus spongiarum OX=431041 GN=FLM9_491 PE=3 SV=1
MM1 pKa = 7.31TRR3 pKa = 11.84QTLEE7 pKa = 3.63GTSRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84KK14 pKa = 7.95RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1661

0

1661

429182

21

2476

258.4

28.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.542 ± 0.086

1.389 ± 0.023

4.989 ± 0.044

5.262 ± 0.065

2.987 ± 0.039

8.329 ± 0.067

2.635 ± 0.036

3.813 ± 0.048

2.598 ± 0.059

12.424 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.029

2.479 ± 0.036

5.876 ± 0.052

5.25 ± 0.051

7.56 ± 0.057

5.544 ± 0.048

5.103 ± 0.037

7.456 ± 0.05

1.694 ± 0.034

1.86 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski