Streptococcus satellite phage Javan421

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMW4|A0A4D5ZMW4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan421 OX=2558695 GN=JavanS421_0026 PE=4 SV=1
MM1 pKa = 7.89PEE3 pKa = 4.21FIIVEE8 pKa = 4.28GNNDD12 pKa = 3.55LGEE15 pKa = 4.16FFQIDD20 pKa = 3.88GEE22 pKa = 4.38LFSDD26 pKa = 5.02SEE28 pKa = 4.25LLEE31 pKa = 4.17NLKK34 pKa = 9.95KK35 pKa = 8.13WHH37 pKa = 5.67EE38 pKa = 4.21WEE40 pKa = 4.24VPVIIDD46 pKa = 3.95DD47 pKa = 4.06DD48 pKa = 4.72ANRR51 pKa = 11.84MLSEE55 pKa = 4.88DD56 pKa = 3.76EE57 pKa = 4.25TEE59 pKa = 3.78ILYY62 pKa = 10.54FPTHH66 pKa = 6.66EE67 pKa = 5.43DD68 pKa = 2.91MMDD71 pKa = 3.81YY72 pKa = 10.77IRR74 pKa = 11.84VNKK77 pKa = 9.93GLEE80 pKa = 4.15PLYY83 pKa = 9.24HH84 pKa = 6.48TPNKK88 pKa = 9.79PYY90 pKa = 10.23TIISKK95 pKa = 9.37SEE97 pKa = 3.66WLEE100 pKa = 3.8LLDD103 pKa = 3.6WW104 pKa = 4.77

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZSU5|A0A4D5ZSU5_9VIRU Transcriptional regulator OS=Streptococcus satellite phage Javan421 OX=2558695 GN=JavanS421_0003 PE=4 SV=1
MM1 pKa = 7.08MKK3 pKa = 10.37EE4 pKa = 3.95HH5 pKa = 6.38MKK7 pKa = 10.43HH8 pKa = 5.8YY9 pKa = 10.48NLLSADD15 pKa = 3.81EE16 pKa = 4.36LVAINGGFRR25 pKa = 11.84LAEE28 pKa = 4.16GVAIAGILNAAVGIFNAGYY47 pKa = 10.79KK48 pKa = 10.41FGADD52 pKa = 3.04LAKK55 pKa = 10.31RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 3.57

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

3747

52

401

138.8

15.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.711 ± 0.505

0.507 ± 0.096

5.685 ± 0.349

8.22 ± 0.566

3.763 ± 0.224

4.991 ± 0.33

1.548 ± 0.199

8.247 ± 0.759

8.887 ± 0.503

10.328 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.236

5.978 ± 0.397

2.562 ± 0.221

4.323 ± 0.386

3.896 ± 0.314

6.138 ± 0.584

5.498 ± 0.459

5.631 ± 0.505

1.148 ± 0.187

4.217 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski