Hahella sp. CCB-MM4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Hahellaceae; Hahella; unclassified Hahella

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261GGH0|A0A261GGH0_9GAMM Sodium/proline symporter OS=Hahella sp. CCB-MM4 OX=1926491 GN=BTA51_24845 PE=3 SV=1
MM1 pKa = 6.71QRR3 pKa = 11.84LMIGVFLLLISAGTLADD20 pKa = 3.55WEE22 pKa = 4.7SAGGSNGQARR32 pKa = 11.84CQKK35 pKa = 10.65AYY37 pKa = 9.28STYY40 pKa = 10.4TGAGWKK46 pKa = 10.4VKK48 pKa = 10.03MNYY51 pKa = 10.0NDD53 pKa = 4.64ANQNCLFYY61 pKa = 10.41LWKK64 pKa = 10.36PMEE67 pKa = 4.67SDD69 pKa = 3.14PCEE72 pKa = 4.91SPEE75 pKa = 4.0TWDD78 pKa = 4.5NTTQQCKK85 pKa = 10.56GPDD88 pKa = 4.09PDD90 pKa = 5.44PDD92 pKa = 4.91PDD94 pKa = 5.08PDD96 pKa = 5.05PDD98 pKa = 5.2PDD100 pKa = 5.49PDD102 pKa = 5.96DD103 pKa = 6.56DD104 pKa = 7.2DD105 pKa = 7.88DD106 pKa = 7.03DD107 pKa = 5.13DD108 pKa = 5.14CQDD111 pKa = 4.09KK112 pKa = 11.03AGQHH116 pKa = 5.73AGYY119 pKa = 9.68IYY121 pKa = 10.01EE122 pKa = 4.46EE123 pKa = 4.45KK124 pKa = 10.91SSISKK129 pKa = 10.37GGCQASCASTGQVIEE144 pKa = 4.32YY145 pKa = 10.31QDD147 pKa = 3.67GTTKK151 pKa = 10.66PIYY154 pKa = 8.63QCIYY158 pKa = 8.74TGEE161 pKa = 4.07EE162 pKa = 3.83AGNDD166 pKa = 3.64DD167 pKa = 4.78GTDD170 pKa = 3.32NDD172 pKa = 4.48NPDD175 pKa = 4.02GGDD178 pKa = 3.55GGDD181 pKa = 3.73GGSGGGDD188 pKa = 2.96GGGDD192 pKa = 3.3GGGDD196 pKa = 3.26GGGDD200 pKa = 3.57DD201 pKa = 4.14TGSGGGDD208 pKa = 3.1GGGDD212 pKa = 3.69GDD214 pKa = 5.5DD215 pKa = 4.41SGSGDD220 pKa = 4.06GDD222 pKa = 3.77DD223 pKa = 4.34TGSGGDD229 pKa = 3.65DD230 pKa = 3.25TGSGGGSGGTGADD243 pKa = 3.16LCQGNDD249 pKa = 3.29LCDD252 pKa = 5.52DD253 pKa = 3.59IAHH256 pKa = 6.75IKK258 pKa = 8.13ATNEE262 pKa = 3.9LIADD266 pKa = 3.63NTARR270 pKa = 11.84IHH272 pKa = 7.63DD273 pKa = 4.34DD274 pKa = 3.16LGEE277 pKa = 4.91IGEE280 pKa = 4.32LLEE283 pKa = 5.57LKK285 pKa = 10.26NDD287 pKa = 3.91HH288 pKa = 6.07QDD290 pKa = 3.42KK291 pKa = 10.96EE292 pKa = 4.49PGSFNSDD299 pKa = 3.07GEE301 pKa = 4.06QRR303 pKa = 11.84IAEE306 pKa = 4.45AEE308 pKa = 4.0AKK310 pKa = 9.93LQGVINGIKK319 pKa = 10.11SEE321 pKa = 4.06FSSLFNLSLSSGSMACSFTMDD342 pKa = 4.32FYY344 pKa = 11.83GEE346 pKa = 4.21TLSFCPNQYY355 pKa = 10.0QEE357 pKa = 4.03QLAIIGPVVMLIGGLVSLNILFRR380 pKa = 5.6

Molecular weight: 39.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261GP66|A0A261GP66_9GAMM Uncharacterized protein OS=Hahella sp. CCB-MM4 OX=1926491 GN=BTA51_12460 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5826

0

5826

1911618

29

5552

328.1

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.454 ± 0.033

1.003 ± 0.009

5.756 ± 0.026

6.531 ± 0.031

3.925 ± 0.02

7.265 ± 0.029

2.266 ± 0.018

5.969 ± 0.023

4.544 ± 0.03

10.389 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.017

3.842 ± 0.02

4.377 ± 0.02

4.35 ± 0.026

5.331 ± 0.029

6.868 ± 0.032

5.22 ± 0.032

6.937 ± 0.027

1.349 ± 0.014

3.106 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski