Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Xanthobacteraceae; Starkeya; Starkeya novella

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4424 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7AAR4|D7AAR4_STAND Ribulose-bisphosphate carboxylase OS=Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456) OX=639283 GN=Snov_3661 PE=3 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84YY3 pKa = 9.57AAVLSAAVLMLGAFPAAAQQSITVRR28 pKa = 11.84PQPRR32 pKa = 11.84AGANGGLEE40 pKa = 3.85ITIPPINNPNYY51 pKa = 10.37LDD53 pKa = 3.73YY54 pKa = 11.48GPLRR58 pKa = 11.84DD59 pKa = 4.88HH60 pKa = 7.42PGADD64 pKa = 3.12HH65 pKa = 6.51SQDD68 pKa = 3.44YY69 pKa = 9.64MNQAGGDD76 pKa = 3.82SMGDD80 pKa = 3.36SGPGSDD86 pKa = 5.21NYY88 pKa = 11.7ADD90 pKa = 5.23LLPDD94 pKa = 3.69SDD96 pKa = 4.0GAYY99 pKa = 10.36DD100 pKa = 4.05GPIPGTPFF108 pKa = 3.34

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7A2Z5|D7A2Z5_STAND Transcriptional regulator AraC family OS=Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456) OX=639283 GN=Snov_0380 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 9.19IINARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 4.99GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4424

0

4424

1400589

31

2843

316.6

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.414 ± 0.052

0.765 ± 0.012

5.445 ± 0.027

5.789 ± 0.033

3.692 ± 0.022

8.891 ± 0.04

1.983 ± 0.018

4.918 ± 0.023

2.918 ± 0.028

10.38 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.017

2.301 ± 0.02

5.624 ± 0.035

2.721 ± 0.023

7.353 ± 0.039

5.006 ± 0.022

5.145 ± 0.027

7.828 ± 0.029

1.307 ± 0.015

2.154 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski