Buchnera aphidicola subsp. Tuberolachnus salignus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A170PBF5|A0A170PBF5_BUCTT Membrane protein insertase YidC OS=Buchnera aphidicola subsp. Tuberolachnus salignus OX=98804 GN=yidC PE=3 SV=1
MM1 pKa = 7.7PKK3 pKa = 9.92IFFYY7 pKa = 9.64PHH9 pKa = 7.25KK10 pKa = 10.25ILLPFGAVISAKK22 pKa = 10.01PGQSILNVALEE33 pKa = 3.78NHH35 pKa = 6.35INIEE39 pKa = 4.22HH40 pKa = 6.85ACEE43 pKa = 3.94QSCACCTCHH52 pKa = 7.39CIIRR56 pKa = 11.84KK57 pKa = 8.71GFNSLSICLEE67 pKa = 3.99NEE69 pKa = 3.39EE70 pKa = 5.24DD71 pKa = 4.94LLDD74 pKa = 4.24KK75 pKa = 11.1AWGLEE80 pKa = 3.99KK81 pKa = 10.7YY82 pKa = 10.56SRR84 pKa = 11.84LACQAKK90 pKa = 10.28LGEE93 pKa = 4.16EE94 pKa = 4.45DD95 pKa = 3.91IEE97 pKa = 4.58VEE99 pKa = 3.8IPIYY103 pKa = 10.38NLHH106 pKa = 5.94QKK108 pKa = 9.77IEE110 pKa = 4.01

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160SVP3|A0A160SVP3_BUCTT 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Buchnera aphidicola subsp. Tuberolachnus salignus OX=98804 GN=metE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 8.93RR14 pKa = 11.84SHH16 pKa = 6.2GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.01NGKK28 pKa = 9.93CILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.69LRR39 pKa = 11.84RR40 pKa = 11.84ILSAA44 pKa = 3.99

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

382

0

382

124381

38

1417

325.6

37.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.346 ± 0.119

1.267 ± 0.037

3.317 ± 0.077

4.564 ± 0.103

6.268 ± 0.188

4.766 ± 0.132

2.118 ± 0.049

12.286 ± 0.141

12.393 ± 0.234

10.7 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.95 ± 0.058

7.198 ± 0.105

2.907 ± 0.058

3.677 ± 0.061

3.046 ± 0.088

6.654 ± 0.11

4.695 ± 0.073

4.045 ± 0.113

0.845 ± 0.041

3.956 ± 0.082
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski