Streptococcus satellite phage Javan581

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRR8|A0A4D5ZRR8_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan581 OX=2558757 GN=JavanS581_0014 PE=4 SV=1
MM1 pKa = 7.67TDD3 pKa = 3.07DD4 pKa = 3.59TEE6 pKa = 4.18IRR8 pKa = 11.84KK9 pKa = 8.93LFQIYY14 pKa = 7.31QTTRR18 pKa = 11.84EE19 pKa = 4.26NKK21 pKa = 10.38DD22 pKa = 2.94MTLNTFSDD30 pKa = 3.76TANTFTFNYY39 pKa = 8.15TFKK42 pKa = 11.11DD43 pKa = 3.04HH44 pKa = 7.52DD45 pKa = 3.88TAQVAGHH52 pKa = 6.91ALMGYY57 pKa = 6.39MTGTFEE63 pKa = 4.03QPGIEE68 pKa = 4.09VYY70 pKa = 10.97YY71 pKa = 11.08DD72 pKa = 3.31NDD74 pKa = 4.04KK75 pKa = 11.67VGGDD79 pKa = 3.8YY80 pKa = 11.12NRR82 pKa = 11.84LAVEE86 pKa = 4.09YY87 pKa = 10.78VADD90 pKa = 4.0TEE92 pKa = 4.19LTEE95 pKa = 4.12TFRR98 pKa = 11.84RR99 pKa = 11.84ICDD102 pKa = 3.58SFQGYY107 pKa = 10.2YY108 pKa = 10.3NDD110 pKa = 4.91PEE112 pKa = 6.04AEE114 pKa = 4.06TDD116 pKa = 3.75VEE118 pKa = 4.02DD119 pKa = 3.49QYY121 pKa = 11.87RR122 pKa = 11.84LEE124 pKa = 4.22RR125 pKa = 11.84VEE127 pKa = 3.91QLKK130 pKa = 10.42QSEE133 pKa = 4.72TFDD136 pKa = 3.59SLLEE140 pKa = 3.94KK141 pKa = 10.66VVIYY145 pKa = 10.18EE146 pKa = 5.3LEE148 pKa = 4.06LLDD151 pKa = 3.69YY152 pKa = 11.22AEE154 pKa = 5.48RR155 pKa = 11.84LLSDD159 pKa = 4.65DD160 pKa = 5.61PIPTDD165 pKa = 3.09TEE167 pKa = 3.97MAYY170 pKa = 7.69MTLNLIGGKK179 pKa = 10.05GVGLFKK185 pKa = 11.15SLDD188 pKa = 3.51EE189 pKa = 4.84DD190 pKa = 3.81NEE192 pKa = 4.35YY193 pKa = 11.31SGLAYY198 pKa = 10.92YY199 pKa = 10.23NAEE202 pKa = 4.13AEE204 pKa = 4.28

Molecular weight: 23.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZR50|A0A4D5ZR50_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan581 OX=2558757 GN=JavanS581_0005 PE=4 SV=1
MM1 pKa = 7.38IMKK4 pKa = 8.9ITEE7 pKa = 4.06VKK9 pKa = 9.83KK10 pKa = 10.92KK11 pKa = 10.69NGATVYY17 pKa = 10.17RR18 pKa = 11.84ASVYY22 pKa = 10.76LGIDD26 pKa = 4.16AITGKK31 pKa = 10.19KK32 pKa = 10.01VKK34 pKa = 10.03TNVTGRR40 pKa = 11.84TKK42 pKa = 10.82KK43 pKa = 9.46EE44 pKa = 4.12VKK46 pKa = 10.43NKK48 pKa = 6.58TQQAIATFKK57 pKa = 10.34TDD59 pKa = 2.86GATRR63 pKa = 11.84YY64 pKa = 9.71QNATITSYY72 pKa = 11.5KK73 pKa = 10.12EE74 pKa = 4.02LAEE77 pKa = 4.25LWWNSYY83 pKa = 8.4KK84 pKa = 9.73HH85 pKa = 4.85TVKK88 pKa = 10.64PNTRR92 pKa = 11.84GNIRR96 pKa = 11.84GLLKK100 pKa = 10.74NHH102 pKa = 6.69VIPLFGAYY110 pKa = 9.51KK111 pKa = 10.15LDD113 pKa = 3.71KK114 pKa = 10.24LTTPLLQSIVIKK126 pKa = 10.71LADD129 pKa = 3.5KK130 pKa = 11.26ANTGEE135 pKa = 4.04AGAYY139 pKa = 8.96LHH141 pKa = 6.56YY142 pKa = 11.05DD143 pKa = 4.09KK144 pKa = 11.05IHH146 pKa = 6.58ALNKK150 pKa = 10.18RR151 pKa = 11.84ILQYY155 pKa = 10.87GVVMQVLPYY164 pKa = 9.81NPARR168 pKa = 11.84EE169 pKa = 4.25VILPRR174 pKa = 11.84NAKK177 pKa = 9.33KK178 pKa = 9.43ATRR181 pKa = 11.84KK182 pKa = 9.25KK183 pKa = 10.03VKK185 pKa = 10.16HH186 pKa = 6.24FNDD189 pKa = 3.34EE190 pKa = 3.86QLKK193 pKa = 10.06QFLGYY198 pKa = 10.59LDD200 pKa = 5.41GLDD203 pKa = 3.24LTNYY207 pKa = 9.51RR208 pKa = 11.84NLYY211 pKa = 9.16EE212 pKa = 3.97ATLYY216 pKa = 10.98KK217 pKa = 10.43FLLATGCRR225 pKa = 11.84INEE228 pKa = 3.89VLALHH233 pKa = 6.87WSDD236 pKa = 4.12IDD238 pKa = 4.14LNNATVSITKK248 pKa = 8.91TLNRR252 pKa = 11.84YY253 pKa = 9.22GSINSPKK260 pKa = 9.97SNASIRR266 pKa = 11.84DD267 pKa = 3.53INIDD271 pKa = 3.67SQTVAMMKK279 pKa = 9.85EE280 pKa = 3.79YY281 pKa = 10.34RR282 pKa = 11.84RR283 pKa = 11.84RR284 pKa = 11.84QIQEE288 pKa = 2.98AWTLGRR294 pKa = 11.84SEE296 pKa = 4.42TVVFSDD302 pKa = 5.82FIHH305 pKa = 7.8DD306 pKa = 3.96YY307 pKa = 11.31PEE309 pKa = 5.01DD310 pKa = 3.47KK311 pKa = 10.71TLGNRR316 pKa = 11.84LTTRR320 pKa = 11.84LRR322 pKa = 11.84NIGLPNIGFHH332 pKa = 6.38GFRR335 pKa = 11.84HH336 pKa = 4.87THH338 pKa = 6.58ASLLLNSGIPYY349 pKa = 10.0KK350 pKa = 10.34EE351 pKa = 3.8LQHH354 pKa = 6.73RR355 pKa = 11.84LGHH358 pKa = 5.38SRR360 pKa = 11.84ISITMDD366 pKa = 2.92IYY368 pKa = 11.54SHH370 pKa = 7.04LSKK373 pKa = 10.96EE374 pKa = 4.11NAKK377 pKa = 10.48NAVAFYY383 pKa = 10.59EE384 pKa = 4.29KK385 pKa = 10.97ALGNLL390 pKa = 3.69

Molecular weight: 44.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

3120

47

447

183.5

21.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.282 ± 0.453

0.577 ± 0.122

5.865 ± 0.612

8.013 ± 0.691

3.654 ± 0.326

4.327 ± 0.378

1.923 ± 0.427

7.115 ± 0.439

8.622 ± 0.716

10.192 ± 0.462

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.383

6.026 ± 0.582

2.821 ± 0.173

4.712 ± 0.43

5.385 ± 0.396

5.096 ± 0.571

6.41 ± 0.527

4.551 ± 0.274

0.865 ± 0.151

4.808 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski