Escherichia phage HZ2R8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Escherichia virus HZ2R8

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VJZ6|A0A2K9VJZ6_9CAUD Tail fiber protein OS=Escherichia phage HZ2R8 OX=2079317 GN=HZ2R8_38 PE=4 SV=1
MM1 pKa = 7.72ANNTTQYY8 pKa = 11.35GLTAQTVLFYY18 pKa = 11.2SDD20 pKa = 3.48MVRR23 pKa = 11.84CGFDD27 pKa = 2.5WSLAMEE33 pKa = 4.58HH34 pKa = 6.59LKK36 pKa = 10.47EE37 pKa = 4.34LYY39 pKa = 10.14EE40 pKa = 4.08NNKK43 pKa = 10.29AIALEE48 pKa = 4.19SAEE51 pKa = 4.07

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VK00|A0A2K9VK00_9CAUD DNA primase/helicase OS=Escherichia phage HZ2R8 OX=2079317 GN=HZ2R8_16 PE=4 SV=1
MM1 pKa = 7.29VRR3 pKa = 11.84RR4 pKa = 11.84LRR6 pKa = 11.84LWTEE10 pKa = 3.79RR11 pKa = 11.84CSFRR15 pKa = 11.84PLLTAQTVLLLLLYY29 pKa = 10.5VKK31 pKa = 10.48DD32 pKa = 3.24SAMPSRR38 pKa = 11.84RR39 pKa = 11.84CSKK42 pKa = 7.74RR43 pKa = 11.84WMMRR47 pKa = 11.84PCSKK51 pKa = 10.36HH52 pKa = 5.09PQPLYY57 pKa = 10.78GRR59 pKa = 11.84CSDD62 pKa = 3.57VV63 pKa = 2.95

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

11149

51

1318

293.4

32.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.283 ± 0.456

1.139 ± 0.193

6.413 ± 0.238

7.364 ± 0.397

3.444 ± 0.208

7.364 ± 0.309

1.875 ± 0.214

5.265 ± 0.179

6.494 ± 0.371

8.037 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.041 ± 0.192

4.61 ± 0.35

3.57 ± 0.187

3.839 ± 0.324

5.848 ± 0.232

5.947 ± 0.299

5.418 ± 0.199

6.225 ± 0.232

1.525 ± 0.154

3.301 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski