Oceanicola granulosus (strain ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Oceanicola; Oceanicola granulosus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2CKD9|Q2CKD9_OCEGH Putative cyclopropane/cyclopropene fatty acid synthesis protein OS=Oceanicola granulosus (strain ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516) OX=314256 GN=OG2516_10321 PE=4 SV=1
MM1 pKa = 7.44TFTLRR6 pKa = 11.84FATASILAMTASAAAAQDD24 pKa = 4.34AIFSQPVEE32 pKa = 4.4DD33 pKa = 4.11RR34 pKa = 11.84NEE36 pKa = 3.83ALTEE40 pKa = 3.93QIEE43 pKa = 4.22EE44 pKa = 4.12DD45 pKa = 3.96FEE47 pKa = 6.62RR48 pKa = 11.84DD49 pKa = 3.19TTFGNEE55 pKa = 3.46GRR57 pKa = 11.84QLGFDD62 pKa = 3.47GSVALRR68 pKa = 11.84ASAASGNTDD77 pKa = 3.11NADD80 pKa = 3.17IGVGANFGYY89 pKa = 10.71FDD91 pKa = 3.52GTNGYY96 pKa = 9.22EE97 pKa = 4.19LGLSYY102 pKa = 10.73QYY104 pKa = 11.37GEE106 pKa = 4.49EE107 pKa = 3.95EE108 pKa = 4.64GEE110 pKa = 4.36TVEE113 pKa = 5.72EE114 pKa = 4.04SLLYY118 pKa = 10.54DD119 pKa = 4.11LEE121 pKa = 4.31YY122 pKa = 10.91TRR124 pKa = 11.84DD125 pKa = 3.69FTPRR129 pKa = 11.84FYY131 pKa = 11.48GFAKK135 pKa = 10.35LQGSIDD141 pKa = 3.65EE142 pKa = 4.64FSSFEE147 pKa = 3.89NDD149 pKa = 2.88TFLGFGAGYY158 pKa = 9.91RR159 pKa = 11.84VVNTATTQWSLQAGPGYY176 pKa = 10.11RR177 pKa = 11.84VAEE180 pKa = 4.26LSDD183 pKa = 3.58AVDD186 pKa = 3.5RR187 pKa = 11.84DD188 pKa = 3.89FEE190 pKa = 4.15EE191 pKa = 4.55AALSVTSNYY200 pKa = 7.67YY201 pKa = 9.75TEE203 pKa = 4.19VANGISLTNDD213 pKa = 2.67TDD215 pKa = 4.28IIASEE220 pKa = 4.34SDD222 pKa = 3.3TVVYY226 pKa = 10.93NDD228 pKa = 4.17LGLNVAMNNALALRR242 pKa = 11.84TSVQTEE248 pKa = 4.24YY249 pKa = 9.05HH250 pKa = 5.77TEE252 pKa = 3.82PARR255 pKa = 11.84GMDD258 pKa = 4.0DD259 pKa = 3.1TDD261 pKa = 3.13NTYY264 pKa = 10.88GVSLVYY270 pKa = 10.75SFNN273 pKa = 3.65

Molecular weight: 29.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2CI31|Q2CI31_OCEGH Uncharacterized protein OS=Oceanicola granulosus (strain ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516) OX=314256 GN=OG2516_08117 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.53ILNNRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.05SLSAA44 pKa = 3.93

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3791

0

3791

1231118

21

12869

324.7

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.572 ± 0.07

0.785 ± 0.018

6.244 ± 0.091

6.3 ± 0.047

3.501 ± 0.029

9.226 ± 0.088

2.046 ± 0.027

4.546 ± 0.029

2.183 ± 0.035

10.367 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.038

2.168 ± 0.027

5.349 ± 0.05

2.705 ± 0.022

7.5 ± 0.069

4.737 ± 0.048

5.342 ± 0.06

7.523 ± 0.041

1.38 ± 0.022

2.107 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski