Gasterosteus aculeatus (Three-spined stickleback)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3N9K8|G3N9K8_GASAC Uncharacterized protein OS=Gasterosteus aculeatus OX=69293 PE=4 SV=1
AA1 pKa = 7.77RR2 pKa = 11.84PALSCLSCGEE12 pKa = 4.3TVRR15 pKa = 11.84GDD17 pKa = 3.7DD18 pKa = 4.5VVACDD23 pKa = 3.73VPRR26 pKa = 11.84CAGLFCRR33 pKa = 11.84PCFLSLEE40 pKa = 4.49STCAVCTRR48 pKa = 11.84PLTSGEE54 pKa = 4.11EE55 pKa = 4.48GEE57 pKa = 4.75EE58 pKa = 4.06EE59 pKa = 4.63LDD61 pKa = 4.7SSDD64 pKa = 5.9DD65 pKa = 3.64EE66 pKa = 4.38QLNSGAGGSDD76 pKa = 3.38IIDD79 pKa = 3.72SDD81 pKa = 4.25SQLSEE86 pKa = 5.02ADD88 pKa = 3.04MTYY91 pKa = 10.64QDD93 pKa = 4.78RR94 pKa = 11.84PGSDD98 pKa = 4.28DD99 pKa = 4.0SDD101 pKa = 4.6DD102 pKa = 4.26SFHH105 pKa = 6.62SASSRR110 pKa = 11.84RR111 pKa = 11.84SSPQDD116 pKa = 3.09PSLQSVLVHH125 pKa = 6.49APEE128 pKa = 3.83

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3NT18|G3NT18_GASAC Sorting nexin 13 OS=Gasterosteus aculeatus OX=69293 PE=3 SV=1
LL1 pKa = 7.19PPRR4 pKa = 11.84RR5 pKa = 11.84PPSPRR10 pKa = 11.84KK11 pKa = 8.48RR12 pKa = 11.84SQRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.26SRR21 pKa = 11.84RR22 pKa = 11.84SPKK25 pKa = 8.4RR26 pKa = 11.84WRR28 pKa = 11.84PRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84QPRR36 pKa = 11.84VPPQQQLRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84AKK54 pKa = 9.81RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84PDD60 pKa = 2.99KK61 pKa = 10.68PMCLPLSARR70 pKa = 11.84QLRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84NQTKK79 pKa = 10.1QKK81 pKa = 8.71INKK84 pKa = 6.44MLRR87 pKa = 11.84RR88 pKa = 11.84SLKK91 pKa = 8.17QRR93 pKa = 11.84RR94 pKa = 11.84PLRR97 pKa = 11.84QMRR100 pKa = 11.84RR101 pKa = 11.84PRR103 pKa = 11.84APSPRR108 pKa = 11.84MTKK111 pKa = 10.39AKK113 pKa = 10.46SDD115 pKa = 3.62LKK117 pKa = 10.72KK118 pKa = 10.96AEE120 pKa = 3.94LRR122 pKa = 11.84VV123 pKa = 3.48

Molecular weight: 15.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20665

6584

27249

13490636

8

26130

495.1

55.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.997 ± 0.013

2.305 ± 0.014

5.197 ± 0.01

6.644 ± 0.02

3.781 ± 0.012

6.644 ± 0.024

2.608 ± 0.007

4.317 ± 0.012

5.491 ± 0.016

9.69 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.007

3.72 ± 0.01

5.567 ± 0.017

4.501 ± 0.014

5.9 ± 0.014

8.216 ± 0.018

5.436 ± 0.012

6.623 ± 0.013

1.204 ± 0.006

2.793 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski