Paenibacillus cellulosilyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V2YLC8|A0A2V2YLC8_9BACL Uncharacterized protein OS=Paenibacillus cellulosilyticus OX=375489 GN=DFQ01_13147 PE=4 SV=1
MM1 pKa = 7.37TGSASYY7 pKa = 10.95SFSLHH12 pKa = 5.97NNGGGTVVVYY22 pKa = 9.5STMSLPVSDD31 pKa = 5.24PPSLDD36 pKa = 2.92HH37 pKa = 7.5DD38 pKa = 4.6AYY40 pKa = 10.71YY41 pKa = 10.11PIYY44 pKa = 9.61TRR46 pKa = 11.84LGTVDD51 pKa = 3.9SNQSGSFSTGNPLARR66 pKa = 11.84IVIARR71 pKa = 11.84ATDD74 pKa = 3.71DD75 pKa = 4.35FPLKK79 pKa = 10.5LAIVDD84 pKa = 3.93TLDD87 pKa = 4.27PDD89 pKa = 3.93SQTVAVGDD97 pKa = 4.18DD98 pKa = 3.87DD99 pKa = 5.24GKK101 pKa = 10.42ATALAWTFYY110 pKa = 10.8QSYY113 pKa = 10.62ASQPYY118 pKa = 9.38QPLALQFNDD127 pKa = 3.81LVLNNTDD134 pKa = 3.36PTQLNGQAASFFSANGYY151 pKa = 10.54AGIDD155 pKa = 3.34FSIFSMVSFWATNSLYY171 pKa = 10.66AFPGTYY177 pKa = 9.71YY178 pKa = 10.58CYY180 pKa = 10.21EE181 pKa = 4.35PQSDD185 pKa = 3.55THH187 pKa = 7.14GFIVPPDD194 pKa = 3.62EE195 pKa = 4.51VGTITVANGTATYY208 pKa = 9.72TSAADD213 pKa = 3.87GSTQTLLFVRR223 pKa = 11.84SKK225 pKa = 10.83LSSAGADD232 pKa = 3.48SKK234 pKa = 11.76NGIALTSILRR244 pKa = 11.84DD245 pKa = 3.6FAWEE249 pKa = 4.19GKK251 pKa = 8.95PDD253 pKa = 3.56QIGICFVGTNNGAQMIAQPYY273 pKa = 8.38QNPSVPWYY281 pKa = 10.03AVAYY285 pKa = 10.1DD286 pKa = 3.64MVYY289 pKa = 10.85GAFTLVQIAMALDD302 pKa = 3.81MAVHH306 pKa = 6.03MLKK309 pKa = 10.67SIGSGLQYY317 pKa = 11.15LKK319 pKa = 11.28DD320 pKa = 4.03NLGKK324 pKa = 10.7LYY326 pKa = 10.95DD327 pKa = 5.46NIRR330 pKa = 11.84QWANEE335 pKa = 4.24TGDD338 pKa = 3.61SAGPGSGIGDD348 pKa = 3.6DD349 pKa = 3.74VDD351 pKa = 3.89PVNVDD356 pKa = 2.56VDD358 pKa = 3.49IDD360 pKa = 3.7VDD362 pKa = 3.53IDD364 pKa = 3.44VDD366 pKa = 3.58IDD368 pKa = 3.46VDD370 pKa = 3.53IDD372 pKa = 3.17IDD374 pKa = 3.5IDD376 pKa = 3.52IDD378 pKa = 3.78VDD380 pKa = 3.55VDD382 pKa = 3.09IDD384 pKa = 3.42IDD386 pKa = 3.71IDD388 pKa = 3.77VDD390 pKa = 4.02FLAVVDD396 pKa = 3.93VDD398 pKa = 3.58VDD400 pKa = 3.53VDD402 pKa = 3.33IDD404 pKa = 3.43IDD406 pKa = 3.76IDD408 pKa = 4.04VVTDD412 pKa = 3.44TEE414 pKa = 4.34TDD416 pKa = 2.83VDD418 pKa = 3.67IDD420 pKa = 3.7VDD422 pKa = 3.63IDD424 pKa = 3.19IDD426 pKa = 3.85TDD428 pKa = 3.54VDD430 pKa = 3.92VQPGMIRR437 pKa = 11.84GLLNKK442 pKa = 9.54VGSWVMEE449 pKa = 4.07KK450 pKa = 10.6ALPALTEE457 pKa = 3.81MLAINLAFMSAAQLLQAWRR476 pKa = 11.84HH477 pKa = 5.37ADD479 pKa = 3.33EE480 pKa = 4.6QGIKK484 pKa = 10.2NLQPRR489 pKa = 11.84EE490 pKa = 4.16STGLGLLVNYY500 pKa = 7.63MLNEE504 pKa = 3.93QVSLQTRR511 pKa = 11.84WNTFADD517 pKa = 4.19YY518 pKa = 10.46VQQSQADD525 pKa = 3.55NATIEE530 pKa = 4.48MTVSTIIMAGNSAADD545 pKa = 3.43QAASNWRR552 pKa = 11.84WSTDD556 pKa = 3.04AEE558 pKa = 4.27NAAVAALAQYY568 pKa = 9.91TGDD571 pKa = 3.14NRR573 pKa = 11.84YY574 pKa = 8.73QAFQTLGGYY583 pKa = 9.14QYY585 pKa = 10.73QGRR588 pKa = 11.84DD589 pKa = 3.39LPVKK593 pKa = 10.57VGAQVAMDD601 pKa = 3.88YY602 pKa = 10.8LKK604 pKa = 11.04KK605 pKa = 10.87GGG607 pKa = 3.63

Molecular weight: 65.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V2Z1F9|A0A2V2Z1F9_9BACL Uncharacterized protein OS=Paenibacillus cellulosilyticus OX=375489 GN=DFQ01_11095 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.88RR12 pKa = 11.84KK13 pKa = 8.93KK14 pKa = 8.63NHH16 pKa = 4.89GFRR19 pKa = 11.84KK20 pKa = 10.06RR21 pKa = 11.84MASKK25 pKa = 10.64NGRR28 pKa = 11.84KK29 pKa = 9.12VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6112

0

6112

2016309

27

3561

329.9

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.744 ± 0.042

0.76 ± 0.01

5.421 ± 0.026

6.359 ± 0.039

3.902 ± 0.023

7.409 ± 0.029

2.072 ± 0.017

6.51 ± 0.032

4.732 ± 0.029

9.786 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.762 ± 0.02

3.876 ± 0.026

3.963 ± 0.021

3.93 ± 0.022

5.261 ± 0.036

6.66 ± 0.033

5.865 ± 0.048

7.128 ± 0.029

1.321 ± 0.014

3.539 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski