Klebsiella phage ST899-OXA48phi17.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J9B8|A0A482J9B8_9CAUD HNH endonuclease OS=Klebsiella phage ST899-OXA48phi17.2 OX=2510459 PE=4 SV=1
MM1 pKa = 7.48TEE3 pKa = 3.84DD4 pKa = 5.32DD5 pKa = 4.81IYY7 pKa = 11.93NLLSPLADD15 pKa = 2.97GRR17 pKa = 11.84VYY19 pKa = 10.55PYY21 pKa = 10.66VVPLGSDD28 pKa = 3.41NLPAVADD35 pKa = 4.02PYY37 pKa = 10.7IIFSIPTDD45 pKa = 3.27VSGDD49 pKa = 3.84VFCGQAEE56 pKa = 4.54STLHH60 pKa = 5.56IQVDD64 pKa = 3.88VWAKK68 pKa = 9.79TNDD71 pKa = 3.04EE72 pKa = 3.89ARR74 pKa = 11.84ALRR77 pKa = 11.84LEE79 pKa = 4.11ALSRR83 pKa = 11.84LEE85 pKa = 3.71VLSPVEE91 pKa = 3.93VTKK94 pKa = 10.77IPGYY98 pKa = 9.2DD99 pKa = 3.31TTTHH103 pKa = 6.1LHH105 pKa = 6.34RR106 pKa = 11.84ATLEE110 pKa = 3.73ITVISS115 pKa = 3.91

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J6D3|A0A482J6D3_9CAUD Uncharacterized protein OS=Klebsiella phage ST899-OXA48phi17.2 OX=2510459 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 9.83IGPMRR7 pKa = 11.84HH8 pKa = 6.44RR9 pKa = 11.84ITIRR13 pKa = 11.84NFTATRR19 pKa = 11.84TPSGQPVEE27 pKa = 4.02KK28 pKa = 10.19WEE30 pKa = 4.48DD31 pKa = 3.29GKK33 pKa = 9.33TIWAEE38 pKa = 3.78VKK40 pKa = 10.47GISGRR45 pKa = 11.84EE46 pKa = 3.98SLTAGAEE53 pKa = 4.08RR54 pKa = 11.84ADD56 pKa = 3.29ATVRR60 pKa = 11.84VWVRR64 pKa = 11.84YY65 pKa = 9.98RR66 pKa = 11.84NDD68 pKa = 2.54ISASSRR74 pKa = 11.84LLVRR78 pKa = 11.84NGPYY82 pKa = 10.04KK83 pKa = 10.74GVTLNVTGPPVPDD96 pKa = 3.27SKK98 pKa = 10.73GTRR101 pKa = 11.84LEE103 pKa = 4.07ILCKK107 pKa = 10.32QGTEE111 pKa = 4.08KK112 pKa = 11.04

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

5765

83

1194

288.3

31.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.853 ± 0.96

0.729 ± 0.219

6.418 ± 0.532

6.019 ± 0.324

3.348 ± 0.322

7.788 ± 0.307

0.954 ± 0.236

5.325 ± 0.271

4.961 ± 0.525

8.309 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.192

3.868 ± 0.28

4.302 ± 0.606

4.649 ± 0.698

5.69 ± 0.313

7.823 ± 0.482

6.539 ± 0.599

6.418 ± 0.399

1.561 ± 0.284

2.81 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski