Amino acid dipepetide frequency for Gordonia phage DobbysSock

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.614AlaAla: 17.614 ± 1.583
1.036AlaCys: 1.036 ± 0.295
8.742AlaAsp: 8.742 ± 0.635
9.066AlaGlu: 9.066 ± 0.996
2.137AlaPhe: 2.137 ± 0.419
8.613AlaGly: 8.613 ± 0.777
2.396AlaHis: 2.396 ± 0.448
5.634AlaIle: 5.634 ± 0.64
4.922AlaLys: 4.922 ± 0.584
9.973AlaLeu: 9.973 ± 0.848
3.626AlaMet: 3.626 ± 0.643
3.238AlaAsn: 3.238 ± 0.662
4.727AlaPro: 4.727 ± 0.648
5.31AlaGln: 5.31 ± 0.861
8.224AlaArg: 8.224 ± 0.882
6.217AlaSer: 6.217 ± 0.669
8.678AlaThr: 8.678 ± 1.057
8.095AlaVal: 8.095 ± 0.988
1.943AlaTrp: 1.943 ± 0.41
2.331AlaTyr: 2.331 ± 0.351
0.0AlaXaa: 0.0 ± 0.0
Cys
0.842CysAla: 0.842 ± 0.217
0.194CysCys: 0.194 ± 0.11
0.648CysAsp: 0.648 ± 0.235
0.518CysGlu: 0.518 ± 0.216
0.13CysPhe: 0.13 ± 0.092
1.101CysGly: 1.101 ± 0.326
0.259CysHis: 0.259 ± 0.154
0.259CysIle: 0.259 ± 0.116
0.259CysLys: 0.259 ± 0.137
0.389CysLeu: 0.389 ± 0.173
0.065CysMet: 0.065 ± 0.066
0.259CysAsn: 0.259 ± 0.121
0.194CysPro: 0.194 ± 0.109
0.389CysGln: 0.389 ± 0.208
0.907CysArg: 0.907 ± 0.318
0.453CysSer: 0.453 ± 0.197
0.648CysThr: 0.648 ± 0.305
0.324CysVal: 0.324 ± 0.172
0.259CysTrp: 0.259 ± 0.127
0.389CysTyr: 0.389 ± 0.161
0.0CysXaa: 0.0 ± 0.0
Asp
7.382AspAla: 7.382 ± 0.73
0.583AspCys: 0.583 ± 0.205
6.282AspAsp: 6.282 ± 0.804
5.051AspGlu: 5.051 ± 0.742
1.684AspPhe: 1.684 ± 0.363
5.181AspGly: 5.181 ± 0.683
2.396AspHis: 2.396 ± 0.441
2.979AspIle: 2.979 ± 0.496
1.943AspLys: 1.943 ± 0.287
6.735AspLeu: 6.735 ± 0.55
1.23AspMet: 1.23 ± 0.294
1.878AspAsn: 1.878 ± 0.36
5.116AspPro: 5.116 ± 0.529
2.331AspGln: 2.331 ± 0.384
4.986AspArg: 4.986 ± 0.763
2.331AspSer: 2.331 ± 0.408
4.08AspThr: 4.08 ± 0.566
5.31AspVal: 5.31 ± 0.51
1.36AspTrp: 1.36 ± 0.294
1.489AspTyr: 1.489 ± 0.248
0.0AspXaa: 0.0 ± 0.0
Glu
7.123GluAla: 7.123 ± 0.712
0.648GluCys: 0.648 ± 0.224
3.108GluAsp: 3.108 ± 0.508
2.785GluGlu: 2.785 ± 0.511
2.267GluPhe: 2.267 ± 0.432
3.626GluGly: 3.626 ± 0.538
1.23GluHis: 1.23 ± 0.411
2.59GluIle: 2.59 ± 0.417
1.748GluLys: 1.748 ± 0.394
6.023GluLeu: 6.023 ± 0.552
1.36GluMet: 1.36 ± 0.377
1.36GluAsn: 1.36 ± 0.303
2.461GluPro: 2.461 ± 0.457
3.497GluGln: 3.497 ± 0.553
5.181GluArg: 5.181 ± 0.707
3.562GluSer: 3.562 ± 0.491
3.691GluThr: 3.691 ± 0.465
4.404GluVal: 4.404 ± 0.569
1.166GluTrp: 1.166 ± 0.25
1.813GluTyr: 1.813 ± 0.368
0.0GluXaa: 0.0 ± 0.0
Phe
2.849PheAla: 2.849 ± 0.42
0.065PheCys: 0.065 ± 0.064
2.396PheAsp: 2.396 ± 0.337
1.619PheGlu: 1.619 ± 0.361
0.518PhePhe: 0.518 ± 0.178
2.655PheGly: 2.655 ± 0.329
0.648PheHis: 0.648 ± 0.239
0.648PheIle: 0.648 ± 0.214
0.324PheLys: 0.324 ± 0.164
1.36PheLeu: 1.36 ± 0.305
0.389PheMet: 0.389 ± 0.136
0.583PheAsn: 0.583 ± 0.243
1.101PhePro: 1.101 ± 0.213
0.777PheGln: 0.777 ± 0.228
1.684PheArg: 1.684 ± 0.38
1.425PheSer: 1.425 ± 0.263
2.202PheThr: 2.202 ± 0.388
1.943PheVal: 1.943 ± 0.444
0.712PheTrp: 0.712 ± 0.223
0.389PheTyr: 0.389 ± 0.156
0.0PheXaa: 0.0 ± 0.0
Gly
8.483GlyAla: 8.483 ± 0.937
0.389GlyCys: 0.389 ± 0.181
5.181GlyAsp: 5.181 ± 0.587
4.209GlyGlu: 4.209 ± 0.419
2.008GlyPhe: 2.008 ± 0.327
7.123GlyGly: 7.123 ± 1.025
1.748GlyHis: 1.748 ± 0.369
3.626GlyIle: 3.626 ± 0.384
3.173GlyLys: 3.173 ± 0.467
6.217GlyLeu: 6.217 ± 0.58
2.008GlyMet: 2.008 ± 0.401
2.849GlyAsn: 2.849 ± 0.456
4.145GlyPro: 4.145 ± 0.539
2.526GlyGln: 2.526 ± 0.584
5.634GlyArg: 5.634 ± 0.584
4.663GlySer: 4.663 ± 0.697
5.958GlyThr: 5.958 ± 0.784
7.123GlyVal: 7.123 ± 0.763
1.489GlyTrp: 1.489 ± 0.33
1.943GlyTyr: 1.943 ± 0.25
0.0GlyXaa: 0.0 ± 0.0
His
2.008HisAla: 2.008 ± 0.408
0.324HisCys: 0.324 ± 0.162
1.101HisAsp: 1.101 ± 0.296
1.101HisGlu: 1.101 ± 0.297
0.324HisPhe: 0.324 ± 0.148
2.267HisGly: 2.267 ± 0.367
0.518HisHis: 0.518 ± 0.196
1.23HisIle: 1.23 ± 0.401
0.648HisLys: 0.648 ± 0.186
1.619HisLeu: 1.619 ± 0.435
0.453HisMet: 0.453 ± 0.154
0.389HisAsn: 0.389 ± 0.166
1.619HisPro: 1.619 ± 0.388
0.907HisGln: 0.907 ± 0.268
1.813HisArg: 1.813 ± 0.395
0.648HisSer: 0.648 ± 0.234
1.943HisThr: 1.943 ± 0.418
1.489HisVal: 1.489 ± 0.289
0.648HisTrp: 0.648 ± 0.173
0.518HisTyr: 0.518 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
6.735IleAla: 6.735 ± 0.777
0.065IleCys: 0.065 ± 0.069
4.339IleAsp: 4.339 ± 0.502
3.367IleGlu: 3.367 ± 0.614
0.648IlePhe: 0.648 ± 0.206
3.562IleGly: 3.562 ± 0.651
0.971IleHis: 0.971 ± 0.256
1.36IleIle: 1.36 ± 0.279
0.907IleLys: 0.907 ± 0.284
2.331IleLeu: 2.331 ± 0.453
0.453IleMet: 0.453 ± 0.172
1.554IleAsn: 1.554 ± 0.364
2.655IlePro: 2.655 ± 0.383
1.166IleGln: 1.166 ± 0.32
3.432IleArg: 3.432 ± 0.407
2.331IleSer: 2.331 ± 0.405
3.173IleThr: 3.173 ± 0.526
3.691IleVal: 3.691 ± 0.456
0.518IleTrp: 0.518 ± 0.186
1.36IleTyr: 1.36 ± 0.356
0.0IleXaa: 0.0 ± 0.0
Lys
4.339LysAla: 4.339 ± 0.605
0.13LysCys: 0.13 ± 0.087
1.748LysAsp: 1.748 ± 0.348
1.684LysGlu: 1.684 ± 0.335
0.648LysPhe: 0.648 ± 0.22
2.137LysGly: 2.137 ± 0.44
0.842LysHis: 0.842 ± 0.234
1.295LysIle: 1.295 ± 0.309
1.23LysLys: 1.23 ± 0.331
3.108LysLeu: 3.108 ± 0.436
0.259LysMet: 0.259 ± 0.12
1.101LysAsn: 1.101 ± 0.227
2.396LysPro: 2.396 ± 0.467
1.36LysGln: 1.36 ± 0.46
2.331LysArg: 2.331 ± 0.414
1.878LysSer: 1.878 ± 0.382
2.008LysThr: 2.008 ± 0.368
2.072LysVal: 2.072 ± 0.322
0.712LysTrp: 0.712 ± 0.251
0.842LysTyr: 0.842 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
10.232LeuAla: 10.232 ± 0.834
0.453LeuCys: 0.453 ± 0.185
6.605LeuAsp: 6.605 ± 0.804
4.145LeuGlu: 4.145 ± 0.567
2.137LeuPhe: 2.137 ± 0.487
5.828LeuGly: 5.828 ± 0.609
1.554LeuHis: 1.554 ± 0.405
3.173LeuIle: 3.173 ± 0.446
2.526LeuLys: 2.526 ± 0.442
5.31LeuLeu: 5.31 ± 0.628
2.072LeuMet: 2.072 ± 0.368
2.59LeuAsn: 2.59 ± 0.511
3.886LeuPro: 3.886 ± 0.564
3.044LeuGln: 3.044 ± 0.541
6.346LeuArg: 6.346 ± 0.684
4.274LeuSer: 4.274 ± 0.466
6.735LeuThr: 6.735 ± 0.71
5.828LeuVal: 5.828 ± 0.861
0.971LeuTrp: 0.971 ± 0.218
1.166LeuTyr: 1.166 ± 0.253
0.0LeuXaa: 0.0 ± 0.0
Met
2.979MetAla: 2.979 ± 0.554
0.194MetCys: 0.194 ± 0.128
1.036MetAsp: 1.036 ± 0.285
0.583MetGlu: 0.583 ± 0.204
0.324MetPhe: 0.324 ± 0.127
1.36MetGly: 1.36 ± 0.361
0.648MetHis: 0.648 ± 0.172
0.842MetIle: 0.842 ± 0.29
0.907MetLys: 0.907 ± 0.183
1.489MetLeu: 1.489 ± 0.291
0.648MetMet: 0.648 ± 0.184
0.777MetAsn: 0.777 ± 0.25
1.489MetPro: 1.489 ± 0.241
0.648MetGln: 0.648 ± 0.198
2.072MetArg: 2.072 ± 0.385
1.36MetSer: 1.36 ± 0.311
2.461MetThr: 2.461 ± 0.368
1.036MetVal: 1.036 ± 0.216
0.583MetTrp: 0.583 ± 0.206
0.194MetTyr: 0.194 ± 0.1
0.0MetXaa: 0.0 ± 0.0
Asn
3.108AsnAla: 3.108 ± 0.642
0.065AsnCys: 0.065 ± 0.064
1.878AsnAsp: 1.878 ± 0.426
1.554AsnGlu: 1.554 ± 0.263
0.518AsnPhe: 0.518 ± 0.163
3.044AsnGly: 3.044 ± 0.606
0.842AsnHis: 0.842 ± 0.204
1.489AsnIle: 1.489 ± 0.24
1.166AsnLys: 1.166 ± 0.376
1.943AsnLeu: 1.943 ± 0.334
0.453AsnMet: 0.453 ± 0.141
0.907AsnAsn: 0.907 ± 0.267
2.267AsnPro: 2.267 ± 0.422
0.971AsnGln: 0.971 ± 0.256
2.461AsnArg: 2.461 ± 0.498
1.943AsnSer: 1.943 ± 0.425
2.072AsnThr: 2.072 ± 0.412
1.36AsnVal: 1.36 ± 0.294
0.389AsnTrp: 0.389 ± 0.136
0.842AsnTyr: 0.842 ± 0.288
0.0AsnXaa: 0.0 ± 0.0
Pro
6.994ProAla: 6.994 ± 0.639
0.583ProCys: 0.583 ± 0.227
4.209ProAsp: 4.209 ± 0.679
3.626ProGlu: 3.626 ± 0.497
0.971ProPhe: 0.971 ± 0.267
5.245ProGly: 5.245 ± 0.813
0.648ProHis: 0.648 ± 0.214
1.878ProIle: 1.878 ± 0.404
1.295ProLys: 1.295 ± 0.256
3.497ProLeu: 3.497 ± 0.436
1.036ProMet: 1.036 ± 0.238
1.748ProAsn: 1.748 ± 0.276
2.461ProPro: 2.461 ± 0.417
1.101ProGln: 1.101 ± 0.267
3.173ProArg: 3.173 ± 0.559
3.238ProSer: 3.238 ± 0.457
4.404ProThr: 4.404 ± 0.62
4.468ProVal: 4.468 ± 0.628
1.425ProTrp: 1.425 ± 0.277
0.907ProTyr: 0.907 ± 0.24
0.0ProXaa: 0.0 ± 0.0
Gln
4.857GlnAla: 4.857 ± 0.778
0.324GlnCys: 0.324 ± 0.178
1.748GlnAsp: 1.748 ± 0.323
1.684GlnGlu: 1.684 ± 0.281
1.101GlnPhe: 1.101 ± 0.287
1.943GlnGly: 1.943 ± 0.602
0.777GlnHis: 0.777 ± 0.223
2.267GlnIle: 2.267 ± 0.41
1.425GlnLys: 1.425 ± 0.334
4.468GlnLeu: 4.468 ± 0.479
1.295GlnMet: 1.295 ± 0.26
0.712GlnAsn: 0.712 ± 0.241
1.166GlnPro: 1.166 ± 0.307
1.489GlnGln: 1.489 ± 0.354
3.691GlnArg: 3.691 ± 0.597
1.813GlnSer: 1.813 ± 0.34
2.72GlnThr: 2.72 ± 0.454
2.849GlnVal: 2.849 ± 0.479
0.777GlnTrp: 0.777 ± 0.202
0.518GlnTyr: 0.518 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
7.706ArgAla: 7.706 ± 0.766
0.777ArgCys: 0.777 ± 0.254
4.663ArgAsp: 4.663 ± 0.719
4.533ArgGlu: 4.533 ± 0.62
2.396ArgPhe: 2.396 ± 0.458
4.792ArgGly: 4.792 ± 0.562
1.489ArgHis: 1.489 ± 0.376
3.821ArgIle: 3.821 ± 0.587
2.461ArgLys: 2.461 ± 0.397
7.123ArgLeu: 7.123 ± 0.668
1.878ArgMet: 1.878 ± 0.315
2.979ArgAsn: 2.979 ± 0.441
4.209ArgPro: 4.209 ± 0.568
2.979ArgGln: 2.979 ± 0.447
6.087ArgArg: 6.087 ± 0.899
3.886ArgSer: 3.886 ± 0.617
4.533ArgThr: 4.533 ± 0.534
4.468ArgVal: 4.468 ± 0.505
2.849ArgTrp: 2.849 ± 0.526
2.008ArgTyr: 2.008 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
6.994SerAla: 6.994 ± 0.682
0.453SerCys: 0.453 ± 0.157
3.367SerAsp: 3.367 ± 0.388
2.979SerGlu: 2.979 ± 0.354
1.36SerPhe: 1.36 ± 0.324
5.828SerGly: 5.828 ± 0.913
0.971SerHis: 0.971 ± 0.26
2.526SerIle: 2.526 ± 0.345
1.619SerLys: 1.619 ± 0.301
3.756SerLeu: 3.756 ± 0.448
1.036SerMet: 1.036 ± 0.311
1.166SerAsn: 1.166 ± 0.338
2.396SerPro: 2.396 ± 0.378
2.59SerGln: 2.59 ± 0.584
3.173SerArg: 3.173 ± 0.594
2.849SerSer: 2.849 ± 0.379
4.08SerThr: 4.08 ± 0.488
3.626SerVal: 3.626 ± 0.501
1.295SerTrp: 1.295 ± 0.329
1.23SerTyr: 1.23 ± 0.33
0.0SerXaa: 0.0 ± 0.0
Thr
9.779ThrAla: 9.779 ± 1.022
0.712ThrCys: 0.712 ± 0.252
5.31ThrAsp: 5.31 ± 0.673
3.562ThrGlu: 3.562 ± 0.449
1.943ThrPhe: 1.943 ± 0.345
6.8ThrGly: 6.8 ± 0.742
1.101ThrHis: 1.101 ± 0.24
4.339ThrIle: 4.339 ± 0.515
1.878ThrLys: 1.878 ± 0.332
5.44ThrLeu: 5.44 ± 0.484
1.295ThrMet: 1.295 ± 0.274
1.813ThrAsn: 1.813 ± 0.389
4.727ThrPro: 4.727 ± 0.785
1.813ThrGln: 1.813 ± 0.406
5.245ThrArg: 5.245 ± 0.88
2.979ThrSer: 2.979 ± 0.599
5.44ThrThr: 5.44 ± 0.711
5.699ThrVal: 5.699 ± 0.607
1.619ThrTrp: 1.619 ± 0.361
1.36ThrTyr: 1.36 ± 0.304
0.0ThrXaa: 0.0 ± 0.0
Val
7.901ValAla: 7.901 ± 0.712
0.712ValCys: 0.712 ± 0.205
5.504ValAsp: 5.504 ± 0.516
5.634ValGlu: 5.634 ± 0.548
1.878ValPhe: 1.878 ± 0.335
5.699ValGly: 5.699 ± 0.702
1.36ValHis: 1.36 ± 0.259
3.562ValIle: 3.562 ± 0.513
2.59ValLys: 2.59 ± 0.418
4.986ValLeu: 4.986 ± 0.531
0.907ValMet: 0.907 ± 0.213
2.461ValAsn: 2.461 ± 0.35
3.303ValPro: 3.303 ± 0.398
2.785ValGln: 2.785 ± 0.509
5.634ValArg: 5.634 ± 0.453
4.533ValSer: 4.533 ± 0.588
5.245ValThr: 5.245 ± 0.583
5.116ValVal: 5.116 ± 0.729
0.777ValTrp: 0.777 ± 0.242
1.295ValTyr: 1.295 ± 0.336
0.0ValXaa: 0.0 ± 0.0
Trp
1.684TrpAla: 1.684 ± 0.342
0.583TrpCys: 0.583 ± 0.21
1.23TrpAsp: 1.23 ± 0.3
0.648TrpGlu: 0.648 ± 0.193
0.648TrpPhe: 0.648 ± 0.196
1.554TrpGly: 1.554 ± 0.268
0.518TrpHis: 0.518 ± 0.171
0.777TrpIle: 0.777 ± 0.179
0.518TrpLys: 0.518 ± 0.173
1.425TrpLeu: 1.425 ± 0.306
0.518TrpMet: 0.518 ± 0.188
0.453TrpAsn: 0.453 ± 0.149
1.36TrpPro: 1.36 ± 0.236
0.907TrpGln: 0.907 ± 0.234
2.267TrpArg: 2.267 ± 0.403
1.425TrpSer: 1.425 ± 0.448
1.489TrpThr: 1.489 ± 0.256
1.295TrpVal: 1.295 ± 0.31
0.648TrpTrp: 0.648 ± 0.2
0.453TrpTyr: 0.453 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.979TyrAla: 2.979 ± 0.422
0.194TyrCys: 0.194 ± 0.109
1.23TyrAsp: 1.23 ± 0.263
1.295TyrGlu: 1.295 ± 0.346
0.648TyrPhe: 0.648 ± 0.172
2.137TyrGly: 2.137 ± 0.296
0.648TyrHis: 0.648 ± 0.251
0.324TyrIle: 0.324 ± 0.141
0.583TyrLys: 0.583 ± 0.198
1.684TyrLeu: 1.684 ± 0.358
0.583TyrMet: 0.583 ± 0.189
0.453TyrAsn: 0.453 ± 0.213
1.23TyrPro: 1.23 ± 0.32
1.036TyrGln: 1.036 ± 0.235
1.295TyrArg: 1.295 ± 0.335
1.489TyrSer: 1.489 ± 0.281
1.295TyrThr: 1.295 ± 0.274
1.684TyrVal: 1.684 ± 0.31
0.259TyrTrp: 0.259 ± 0.128
0.518TyrTyr: 0.518 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (15443 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski