Mycobacterium phage HufflyPuff

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kostyavirus; unclassified Kostyavirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5R853|V5R853_9CAUD Uncharacterized protein OS=Mycobacterium phage HufflyPuff OX=1430411 GN=79 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 6.72DD3 pKa = 4.63DD4 pKa = 6.48DD5 pKa = 5.44LRR7 pKa = 11.84CSCGSLYY14 pKa = 10.72DD15 pKa = 4.55DD16 pKa = 5.69CDD18 pKa = 3.69PDD20 pKa = 3.87YY21 pKa = 11.58CFDD24 pKa = 3.69NPQSYY29 pKa = 9.5FYY31 pKa = 10.1PEE33 pKa = 3.83PRR35 pKa = 11.84TWDD38 pKa = 4.41DD39 pKa = 3.5IEE41 pKa = 6.61DD42 pKa = 5.19DD43 pKa = 3.46IASAFTGQMKK53 pKa = 10.58NLDD56 pKa = 3.4AAFTRR61 pKa = 11.84EE62 pKa = 4.01APKK65 pKa = 10.47YY66 pKa = 10.37APTWWMLVTACPEE79 pKa = 4.6DD80 pKa = 3.59IRR82 pKa = 11.84IEE84 pKa = 4.01QDD86 pKa = 2.89VDD88 pKa = 3.43EE89 pKa = 4.85VDD91 pKa = 3.38YY92 pKa = 9.67MAWGRR97 pKa = 11.84PRR99 pKa = 11.84PVITDD104 pKa = 3.49RR105 pKa = 11.84NLYY108 pKa = 8.92LAWGEE113 pKa = 4.28DD114 pKa = 3.6NRR116 pKa = 11.84HH117 pKa = 6.41PIYY120 pKa = 10.58AYY122 pKa = 9.94FRR124 pKa = 11.84RR125 pKa = 11.84VDD127 pKa = 3.91FDD129 pKa = 4.31KK130 pKa = 11.05QLSWISDD137 pKa = 3.6VQMVEE142 pKa = 4.13PPAWGLWMPSLIGVV156 pKa = 4.11

Molecular weight: 18.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5R7L2|V5R7L2_9CAUD Minor tail protein OS=Mycobacterium phage HufflyPuff OX=1430411 GN=23 PE=4 SV=1
MM1 pKa = 7.43PRR3 pKa = 11.84SAPKK7 pKa = 10.14PCTKK11 pKa = 10.33CGAKK15 pKa = 9.73AARR18 pKa = 11.84RR19 pKa = 11.84GLCARR24 pKa = 11.84HH25 pKa = 6.33DD26 pKa = 4.01RR27 pKa = 11.84QGDD30 pKa = 3.66SHH32 pKa = 7.2RR33 pKa = 11.84GSARR37 pKa = 11.84DD38 pKa = 3.24RR39 pKa = 11.84GYY41 pKa = 11.33DD42 pKa = 3.41SAHH45 pKa = 4.75EE46 pKa = 4.35TEE48 pKa = 5.03FRR50 pKa = 11.84AAVLAAQPTCPCGQPSTVADD70 pKa = 4.48HH71 pKa = 6.54YY72 pKa = 10.19PLSRR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 4.46LIRR81 pKa = 11.84QRR83 pKa = 11.84LNPNDD88 pKa = 3.68PRR90 pKa = 11.84HH91 pKa = 5.6GRR93 pKa = 11.84ALCATCHH100 pKa = 5.09NTHH103 pKa = 6.0TARR106 pKa = 11.84TQSHH110 pKa = 6.83LGHH113 pKa = 7.0RR114 pKa = 11.84SS115 pKa = 2.95

Molecular weight: 12.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

146

0

146

23971

32

1578

164.2

18.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.05 ± 0.397

1.272 ± 0.149

6.479 ± 0.226

6.741 ± 0.277

2.899 ± 0.136

8.727 ± 0.453

2.532 ± 0.145

4.418 ± 0.155

4.43 ± 0.177

8.089 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.119

3.362 ± 0.119

5.373 ± 0.197

3.241 ± 0.197

6.036 ± 0.316

5.653 ± 0.213

6.203 ± 0.212

7.025 ± 0.244

1.982 ± 0.125

3.104 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski