Olsenella profusa F0195

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Atopobiaceae; Olsenella; Olsenella profusa

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|U2TT32|U2TT32_9ACTN ABC transporter transmembrane region domain protein OS=Olsenella profusa F0195 OX=1125712 GN=HMPREF1316_1668 PE=4 SV=1
MM1 pKa = 7.69PWPEE5 pKa = 3.71RR6 pKa = 11.84ALVFLVTLVLALGVGAGVRR25 pKa = 11.84RR26 pKa = 11.84CLAQPTDD33 pKa = 3.74YY34 pKa = 10.81GPDD37 pKa = 3.33VITPTDD43 pKa = 3.53SVTDD47 pKa = 3.77GTAPDD52 pKa = 3.97DD53 pKa = 3.64QGAATDD59 pKa = 3.88AASNAPTSLTAEE71 pKa = 4.4ADD73 pKa = 3.21GGALVLSGDD82 pKa = 3.61VGSRR86 pKa = 11.84TVDD89 pKa = 3.34DD90 pKa = 4.75LSAVVQEE97 pKa = 5.2LDD99 pKa = 3.15ALQDD103 pKa = 3.36GGIQAGVAVTSLDD116 pKa = 3.32GSITLTYY123 pKa = 10.5QQDD126 pKa = 3.32QSFYY130 pKa = 10.35AASTIKK136 pKa = 10.6APYY139 pKa = 7.69VCAVLEE145 pKa = 4.15EE146 pKa = 4.7DD147 pKa = 4.67LPAGTTSMASLRR159 pKa = 11.84DD160 pKa = 3.64AMSSMLLYY168 pKa = 10.52SDD170 pKa = 3.66NDD172 pKa = 3.39SYY174 pKa = 11.69RR175 pKa = 11.84QLRR178 pKa = 11.84DD179 pKa = 3.07SYY181 pKa = 11.59GDD183 pKa = 3.77DD184 pKa = 4.59VFLTWLQRR192 pKa = 11.84HH193 pKa = 6.15DD194 pKa = 4.01VTAGTYY200 pKa = 10.71GSLSDD205 pKa = 4.12YY206 pKa = 11.36ARR208 pKa = 11.84DD209 pKa = 3.79HH210 pKa = 6.77YY211 pKa = 11.07PFSTPEE217 pKa = 3.76QLTQMWQAVWAFVSGDD233 pKa = 3.61AQGASYY239 pKa = 10.96LKK241 pKa = 10.6DD242 pKa = 3.08LLARR246 pKa = 11.84RR247 pKa = 11.84EE248 pKa = 3.96EE249 pKa = 4.3SPIANALGPQTEE261 pKa = 4.9TYY263 pKa = 10.67SKK265 pKa = 10.77AGWYY269 pKa = 9.61PEE271 pKa = 4.13TDD273 pKa = 3.56GSDD276 pKa = 3.21AAPASNDD283 pKa = 3.19AGVVIHH289 pKa = 6.69GSASTSYY296 pKa = 10.14VISVMSTAPSEE307 pKa = 4.08LDD309 pKa = 3.16RR310 pKa = 11.84LEE312 pKa = 4.36GLISAIDD319 pKa = 3.75HH320 pKa = 6.83LMDD323 pKa = 5.46ADD325 pKa = 4.15GAA327 pKa = 4.23

Molecular weight:
34.41 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|U2TID9|U2TID9_9ACTN Uncharacterized protein OS=Olsenella profusa F0195 OX=1125712 GN=HMPREF1316_0657 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MASKK25 pKa = 10.41AGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 8.12QLTVV44 pKa = 2.97

Molecular weight:
5.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2650

0

2650

795533

33

1732

300.2

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.992 ± 0.061

1.578 ± 0.019

6.457 ± 0.042

6.064 ± 0.049

3.287 ± 0.032

8.842 ± 0.049

2.239 ± 0.025

4.663 ± 0.038

2.898 ± 0.036

9.526 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.019

2.512 ± 0.031

4.365 ± 0.037

2.926 ± 0.028

7.244 ± 0.059

5.989 ± 0.042

5.523 ± 0.034

8.177 ± 0.045

1.184 ± 0.02

2.762 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski