Amino acid dipepetide frequency for Lactobacillus rennini DSM 20253

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.519AlaAla: 10.519 ± 0.209
0.483AlaCys: 0.483 ± 0.026
5.422AlaAsp: 5.422 ± 0.101
4.384AlaGlu: 4.384 ± 0.081
3.42AlaPhe: 3.42 ± 0.08
6.658AlaGly: 6.658 ± 0.121
1.986AlaHis: 1.986 ± 0.058
6.121AlaIle: 6.121 ± 0.111
6.401AlaLys: 6.401 ± 0.109
9.561AlaLeu: 9.561 ± 0.145
2.208AlaMet: 2.208 ± 0.063
3.902AlaAsn: 3.902 ± 0.073
3.06AlaPro: 3.06 ± 0.072
5.166AlaGln: 5.166 ± 0.118
3.227AlaArg: 3.227 ± 0.08
4.63AlaSer: 4.63 ± 0.095
5.995AlaThr: 5.995 ± 0.103
6.393AlaVal: 6.393 ± 0.084
0.774AlaTrp: 0.774 ± 0.033
3.004AlaTyr: 3.004 ± 0.066
0.002AlaXaa: 0.002 ± 0.002
Cys
0.458CysAla: 0.458 ± 0.024
0.074CysCys: 0.074 ± 0.011
0.234CysAsp: 0.234 ± 0.02
0.215CysGlu: 0.215 ± 0.019
0.282CysPhe: 0.282 ± 0.02
0.521CysGly: 0.521 ± 0.025
0.192CysHis: 0.192 ± 0.019
0.314CysIle: 0.314 ± 0.022
0.146CysLys: 0.146 ± 0.014
0.66CysLeu: 0.66 ± 0.035
0.09CysMet: 0.09 ± 0.012
0.176CysAsn: 0.176 ± 0.016
0.313CysPro: 0.313 ± 0.025
0.392CysGln: 0.392 ± 0.027
0.257CysArg: 0.257 ± 0.021
0.285CysSer: 0.285 ± 0.02
0.302CysThr: 0.302 ± 0.021
0.394CysVal: 0.394 ± 0.027
0.075CysTrp: 0.075 ± 0.011
0.239CysTyr: 0.239 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
4.02AspAla: 4.02 ± 0.093
0.313AspCys: 0.313 ± 0.024
2.887AspAsp: 2.887 ± 0.097
2.867AspGlu: 2.867 ± 0.084
2.639AspPhe: 2.639 ± 0.061
3.039AspGly: 3.039 ± 0.076
1.347AspHis: 1.347 ± 0.049
3.309AspIle: 3.309 ± 0.072
3.128AspLys: 3.128 ± 0.076
5.402AspLeu: 5.402 ± 0.101
1.368AspMet: 1.368 ± 0.042
1.929AspAsn: 1.929 ± 0.066
2.135AspPro: 2.135 ± 0.058
3.364AspGln: 3.364 ± 0.08
1.922AspArg: 1.922 ± 0.055
2.404AspSer: 2.404 ± 0.06
2.636AspThr: 2.636 ± 0.062
3.673AspVal: 3.673 ± 0.09
0.673AspTrp: 0.673 ± 0.036
2.421AspTyr: 2.421 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
4.026GluAla: 4.026 ± 0.083
0.162GluCys: 0.162 ± 0.016
2.024GluAsp: 2.024 ± 0.064
2.152GluGlu: 2.152 ± 0.078
1.701GluPhe: 1.701 ± 0.053
2.006GluGly: 2.006 ± 0.069
0.991GluHis: 0.991 ± 0.041
3.511GluIle: 3.511 ± 0.081
3.742GluLys: 3.742 ± 0.078
4.832GluLeu: 4.832 ± 0.1
1.474GluMet: 1.474 ± 0.047
2.256GluAsn: 2.256 ± 0.056
1.598GluPro: 1.598 ± 0.054
2.78GluGln: 2.78 ± 0.069
2.205GluArg: 2.205 ± 0.058
2.041GluSer: 2.041 ± 0.056
2.924GluThr: 2.924 ± 0.062
2.902GluVal: 2.902 ± 0.081
0.372GluTrp: 0.372 ± 0.024
1.264GluTyr: 1.264 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.627PheAla: 3.627 ± 0.077
0.282PheCys: 0.282 ± 0.022
2.389PheAsp: 2.389 ± 0.064
1.67PheGlu: 1.67 ± 0.047
1.909PhePhe: 1.909 ± 0.059
3.171PheGly: 3.171 ± 0.077
0.809PheHis: 0.809 ± 0.031
3.106PheIle: 3.106 ± 0.075
2.65PheLys: 2.65 ± 0.071
3.958PheLeu: 3.958 ± 0.1
1.131PheMet: 1.131 ± 0.045
2.179PheAsn: 2.179 ± 0.056
1.471PhePro: 1.471 ± 0.053
1.474PheGln: 1.474 ± 0.049
1.339PheArg: 1.339 ± 0.048
2.895PheSer: 2.895 ± 0.066
2.622PheThr: 2.622 ± 0.065
2.838PheVal: 2.838 ± 0.07
0.513PheTrp: 0.513 ± 0.03
1.675PheTyr: 1.675 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
5.448GlyAla: 5.448 ± 0.105
0.463GlyCys: 0.463 ± 0.03
3.204GlyAsp: 3.204 ± 0.081
2.942GlyGlu: 2.942 ± 0.065
2.981GlyPhe: 2.981 ± 0.071
4.66GlyGly: 4.66 ± 0.113
1.652GlyHis: 1.652 ± 0.051
5.311GlyIle: 5.311 ± 0.105
4.404GlyLys: 4.404 ± 0.087
6.77GlyLeu: 6.77 ± 0.112
1.914GlyMet: 1.914 ± 0.058
2.599GlyAsn: 2.599 ± 0.063
1.735GlyPro: 1.735 ± 0.051
3.359GlyGln: 3.359 ± 0.084
2.755GlyArg: 2.755 ± 0.071
3.473GlySer: 3.473 ± 0.085
3.998GlyThr: 3.998 ± 0.079
4.847GlyVal: 4.847 ± 0.09
0.705GlyTrp: 0.705 ± 0.034
2.737GlyTyr: 2.737 ± 0.058
0.008GlyXaa: 0.008 ± 0.003
His
1.767HisAla: 1.767 ± 0.051
0.149HisCys: 0.149 ± 0.014
1.31HisAsp: 1.31 ± 0.051
1.03HisGlu: 1.03 ± 0.039
1.289HisPhe: 1.289 ± 0.042
1.608HisGly: 1.608 ± 0.043
0.849HisHis: 0.849 ± 0.035
1.315HisIle: 1.315 ± 0.047
1.057HisLys: 1.057 ± 0.041
2.573HisLeu: 2.573 ± 0.07
0.521HisMet: 0.521 ± 0.024
0.907HisAsn: 0.907 ± 0.038
1.126HisPro: 1.126 ± 0.045
1.586HisGln: 1.586 ± 0.053
1.037HisArg: 1.037 ± 0.039
1.06HisSer: 1.06 ± 0.04
1.076HisThr: 1.076 ± 0.046
1.482HisVal: 1.482 ± 0.049
0.322HisTrp: 0.322 ± 0.025
1.269HisTyr: 1.269 ± 0.047
0.0HisXaa: 0.0 ± 0.0
Ile
6.418IleAla: 6.418 ± 0.109
0.515IleCys: 0.515 ± 0.026
3.925IleAsp: 3.925 ± 0.094
2.953IleGlu: 2.953 ± 0.068
2.844IlePhe: 2.844 ± 0.073
5.319IleGly: 5.319 ± 0.115
1.237IleHis: 1.237 ± 0.042
4.72IleIle: 4.72 ± 0.112
4.387IleLys: 4.387 ± 0.09
6.551IleLeu: 6.551 ± 0.127
1.585IleMet: 1.585 ± 0.05
3.108IleAsn: 3.108 ± 0.065
2.74IlePro: 2.74 ± 0.069
2.752IleGln: 2.752 ± 0.069
2.57IleArg: 2.57 ± 0.076
3.872IleSer: 3.872 ± 0.075
4.276IleThr: 4.276 ± 0.071
4.68IleVal: 4.68 ± 0.09
0.596IleTrp: 0.596 ± 0.037
2.191IleTyr: 2.191 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
5.669LysAla: 5.669 ± 0.093
0.179LysCys: 0.179 ± 0.017
2.788LysAsp: 2.788 ± 0.074
3.013LysGlu: 3.013 ± 0.085
1.834LysPhe: 1.834 ± 0.057
3.258LysGly: 3.258 ± 0.08
1.398LysHis: 1.398 ± 0.044
4.386LysIle: 4.386 ± 0.086
4.729LysLys: 4.729 ± 0.108
6.004LysLeu: 6.004 ± 0.096
2.173LysMet: 2.173 ± 0.053
2.846LysAsn: 2.846 ± 0.074
2.538LysPro: 2.538 ± 0.066
4.755LysGln: 4.755 ± 0.091
3.351LysArg: 3.351 ± 0.081
2.968LysSer: 2.968 ± 0.075
4.233LysThr: 4.233 ± 0.071
4.243LysVal: 4.243 ± 0.085
0.567LysTrp: 0.567 ± 0.031
1.909LysTyr: 1.909 ± 0.064
0.0LysXaa: 0.0 ± 0.0
Leu
11.061LeuAla: 11.061 ± 0.159
0.63LeuCys: 0.63 ± 0.033
4.979LeuAsp: 4.979 ± 0.089
4.03LeuGlu: 4.03 ± 0.091
4.436LeuPhe: 4.436 ± 0.111
6.853LeuGly: 6.853 ± 0.125
1.981LeuHis: 1.981 ± 0.056
6.966LeuIle: 6.966 ± 0.129
6.919LeuLys: 6.919 ± 0.126
11.113LeuLeu: 11.113 ± 0.237
2.502LeuMet: 2.502 ± 0.076
5.065LeuAsn: 5.065 ± 0.094
4.786LeuPro: 4.786 ± 0.094
4.853LeuGln: 4.853 ± 0.11
4.222LeuArg: 4.222 ± 0.08
5.885LeuSer: 5.885 ± 0.097
7.823LeuThr: 7.823 ± 0.127
7.195LeuVal: 7.195 ± 0.119
0.875LeuTrp: 0.875 ± 0.047
2.913LeuTyr: 2.913 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
2.666MetAla: 2.666 ± 0.068
0.132MetCys: 0.132 ± 0.014
1.143MetAsp: 1.143 ± 0.048
0.889MetGlu: 0.889 ± 0.04
0.797MetPhe: 0.797 ± 0.029
1.637MetGly: 1.637 ± 0.055
0.493MetHis: 0.493 ± 0.025
1.752MetIle: 1.752 ± 0.051
1.574MetLys: 1.574 ± 0.051
2.525MetLeu: 2.525 ± 0.063
0.794MetMet: 0.794 ± 0.039
1.159MetAsn: 1.159 ± 0.04
1.094MetPro: 1.094 ± 0.034
1.356MetGln: 1.356 ± 0.047
1.174MetArg: 1.174 ± 0.044
1.342MetSer: 1.342 ± 0.049
2.027MetThr: 2.027 ± 0.052
1.706MetVal: 1.706 ± 0.048
0.196MetTrp: 0.196 ± 0.019
0.595MetTyr: 0.595 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.46AsnAla: 3.46 ± 0.073
0.267AsnCys: 0.267 ± 0.024
2.196AsnAsp: 2.196 ± 0.065
2.058AsnGlu: 2.058 ± 0.058
1.865AsnPhe: 1.865 ± 0.05
3.255AsnGly: 3.255 ± 0.092
1.201AsnHis: 1.201 ± 0.047
2.677AsnIle: 2.677 ± 0.068
2.194AsnLys: 2.194 ± 0.068
4.334AsnLeu: 4.334 ± 0.079
1.09AsnMet: 1.09 ± 0.042
1.89AsnAsn: 1.89 ± 0.061
1.939AsnPro: 1.939 ± 0.059
3.076AsnGln: 3.076 ± 0.081
2.052AsnArg: 2.052 ± 0.063
2.106AsnSer: 2.106 ± 0.062
2.282AsnThr: 2.282 ± 0.069
2.938AsnVal: 2.938 ± 0.074
0.628AsnTrp: 0.628 ± 0.032
1.735AsnTyr: 1.735 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
3.728ProAla: 3.728 ± 0.086
0.161ProCys: 0.161 ± 0.017
2.262ProAsp: 2.262 ± 0.056
2.685ProGlu: 2.685 ± 0.07
1.745ProPhe: 1.745 ± 0.048
2.309ProGly: 2.309 ± 0.054
0.929ProHis: 0.929 ± 0.038
2.516ProIle: 2.516 ± 0.064
2.46ProLys: 2.46 ± 0.067
4.208ProLeu: 4.208 ± 0.083
0.691ProMet: 0.691 ± 0.031
1.741ProAsn: 1.741 ± 0.054
0.883ProPro: 0.883 ± 0.041
2.368ProGln: 2.368 ± 0.063
1.398ProArg: 1.398 ± 0.046
1.727ProSer: 1.727 ± 0.05
2.481ProThr: 2.481 ± 0.063
2.974ProVal: 2.974 ± 0.06
0.334ProTrp: 0.334 ± 0.022
1.381ProTyr: 1.381 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
5.946GlnAla: 5.946 ± 0.119
0.167GlnCys: 0.167 ± 0.018
2.044GlnAsp: 2.044 ± 0.068
2.181GlnGlu: 2.181 ± 0.06
2.093GlnPhe: 2.093 ± 0.059
2.683GlnGly: 2.683 ± 0.056
1.696GlnHis: 1.696 ± 0.062
3.666GlnIle: 3.666 ± 0.084
3.784GlnLys: 3.784 ± 0.088
7.422GlnLeu: 7.422 ± 0.161
1.221GlnMet: 1.221 ± 0.04
2.306GlnAsn: 2.306 ± 0.054
2.51GlnPro: 2.51 ± 0.069
5.367GlnGln: 5.367 ± 0.193
3.292GlnArg: 3.292 ± 0.094
2.446GlnSer: 2.446 ± 0.072
3.822GlnThr: 3.822 ± 0.107
4.043GlnVal: 4.043 ± 0.092
0.556GlnTrp: 0.556 ± 0.03
1.794GlnTyr: 1.794 ± 0.056
0.003GlnXaa: 0.003 ± 0.002
Arg
3.088ArgAla: 3.088 ± 0.072
0.244ArgCys: 0.244 ± 0.02
2.164ArgAsp: 2.164 ± 0.066
2.133ArgGlu: 2.133 ± 0.056
2.012ArgPhe: 2.012 ± 0.058
2.346ArgGly: 2.346 ± 0.072
1.289ArgHis: 1.289 ± 0.049
2.63ArgIle: 2.63 ± 0.063
2.55ArgLys: 2.55 ± 0.07
4.959ArgLeu: 4.959 ± 0.101
1.016ArgMet: 1.016 ± 0.046
1.713ArgAsn: 1.713 ± 0.057
1.595ArgPro: 1.595 ± 0.057
3.477ArgGln: 3.477 ± 0.088
2.501ArgArg: 2.501 ± 0.074
1.96ArgSer: 1.96 ± 0.06
2.103ArgThr: 2.103 ± 0.061
2.754ArgVal: 2.754 ± 0.071
0.464ArgTrp: 0.464 ± 0.03
1.897ArgTyr: 1.897 ± 0.06
0.003ArgXaa: 0.003 ± 0.002
Ser
4.556SerAla: 4.556 ± 0.102
0.299SerCys: 0.299 ± 0.021
2.869SerAsp: 2.869 ± 0.067
2.473SerGlu: 2.473 ± 0.064
2.386SerPhe: 2.386 ± 0.056
4.334SerGly: 4.334 ± 0.096
1.185SerHis: 1.185 ± 0.043
3.111SerIle: 3.111 ± 0.077
2.726SerLys: 2.726 ± 0.068
5.457SerLeu: 5.457 ± 0.11
1.273SerMet: 1.273 ± 0.046
1.972SerAsn: 1.972 ± 0.062
1.75SerPro: 1.75 ± 0.055
3.057SerGln: 3.057 ± 0.082
2.222SerArg: 2.222 ± 0.06
2.886SerSer: 2.886 ± 0.1
2.956SerThr: 2.956 ± 0.075
3.552SerVal: 3.552 ± 0.089
0.61SerTrp: 0.61 ± 0.031
1.794SerTyr: 1.794 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
6.491ThrAla: 6.491 ± 0.108
0.287ThrCys: 0.287 ± 0.024
3.652ThrAsp: 3.652 ± 0.087
2.761ThrGlu: 2.761 ± 0.066
2.542ThrPhe: 2.542 ± 0.06
4.547ThrGly: 4.547 ± 0.095
1.349ThrHis: 1.349 ± 0.043
4.438ThrIle: 4.438 ± 0.082
3.871ThrLys: 3.871 ± 0.088
6.343ThrLeu: 6.343 ± 0.112
1.28ThrMet: 1.28 ± 0.05
2.64ThrAsn: 2.64 ± 0.066
2.987ThrPro: 2.987 ± 0.067
3.125ThrGln: 3.125 ± 0.081
2.101ThrArg: 2.101 ± 0.058
3.289ThrSer: 3.289 ± 0.078
4.439ThrThr: 4.439 ± 0.11
4.617ThrVal: 4.617 ± 0.083
0.59ThrTrp: 0.59 ± 0.033
2.126ThrTyr: 2.126 ± 0.053
0.002ThrXaa: 0.002 ± 0.001
Val
6.891ValAla: 6.891 ± 0.107
0.501ValCys: 0.501 ± 0.029
3.658ValAsp: 3.658 ± 0.075
2.85ValGlu: 2.85 ± 0.079
2.594ValPhe: 2.594 ± 0.073
4.689ValGly: 4.689 ± 0.091
1.33ValHis: 1.33 ± 0.048
4.974ValIle: 4.974 ± 0.107
4.561ValLys: 4.561 ± 0.094
6.697ValLeu: 6.697 ± 0.114
1.788ValMet: 1.788 ± 0.056
3.076ValAsn: 3.076 ± 0.079
2.867ValPro: 2.867 ± 0.061
2.765ValGln: 2.765 ± 0.071
2.712ValArg: 2.712 ± 0.075
4.058ValSer: 4.058 ± 0.077
5.141ValThr: 5.141 ± 0.097
5.175ValVal: 5.175 ± 0.102
0.641ValTrp: 0.641 ± 0.034
2.15ValTyr: 2.15 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.699TrpAla: 0.699 ± 0.035
0.063TrpCys: 0.063 ± 0.01
0.435TrpAsp: 0.435 ± 0.028
0.336TrpGlu: 0.336 ± 0.022
0.507TrpPhe: 0.507 ± 0.031
0.616TrpGly: 0.616 ± 0.034
0.343TrpHis: 0.343 ± 0.023
0.524TrpIle: 0.524 ± 0.028
0.314TrpLys: 0.314 ± 0.024
1.554TrpLeu: 1.554 ± 0.055
0.219TrpMet: 0.219 ± 0.016
0.424TrpAsn: 0.424 ± 0.038
0.382TrpPro: 0.382 ± 0.021
0.993TrpGln: 0.993 ± 0.041
0.631TrpArg: 0.631 ± 0.034
0.446TrpSer: 0.446 ± 0.029
0.483TrpThr: 0.483 ± 0.031
0.604TrpVal: 0.604 ± 0.031
0.158TrpTrp: 0.158 ± 0.016
0.354TrpTyr: 0.354 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.775TyrAla: 2.775 ± 0.064
0.251TyrCys: 0.251 ± 0.018
1.89TyrAsp: 1.89 ± 0.061
1.342TyrGlu: 1.342 ± 0.045
1.831TyrPhe: 1.831 ± 0.058
2.455TyrGly: 2.455 ± 0.064
1.076TyrHis: 1.076 ± 0.037
1.891TyrIle: 1.891 ± 0.052
1.244TyrLys: 1.244 ± 0.047
4.162TyrLeu: 4.162 ± 0.084
0.708TyrMet: 0.708 ± 0.035
1.353TyrAsn: 1.353 ± 0.049
1.473TyrPro: 1.473 ± 0.052
2.978TyrGln: 2.978 ± 0.075
1.922TyrArg: 1.922 ± 0.048
1.695TyrSer: 1.695 ± 0.05
1.857TyrThr: 1.857 ± 0.051
2.11TyrVal: 2.11 ± 0.052
0.435TyrTrp: 0.435 ± 0.025
1.46TyrTyr: 1.46 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.002XaaAla: 0.002 ± 0.002
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.002XaaGly: 0.002 ± 0.002
0.0XaaHis: 0.0 ± 0.0
0.002XaaIle: 0.002 ± 0.002
0.0XaaLys: 0.0 ± 0.0
0.003XaaLeu: 0.003 ± 0.002
0.002XaaMet: 0.002 ± 0.002
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.006XaaArg: 0.006 ± 0.003
0.002XaaSer: 0.002 ± 0.002
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.161XaaXaa: 0.161 ± 0.067
Statistics based on 2191 proteins (652553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski