Mycobacterium phage Bruin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kostyavirus; unclassified Kostyavirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5R793|V5R793_9CAUD Uncharacterized protein OS=Mycobacterium phage Bruin OX=1391179 GN=34 PE=4 SV=1
MM1 pKa = 7.61AAGPWTPVNSTRR13 pKa = 11.84TNLLNGTFDD22 pKa = 4.73LDD24 pKa = 3.61SDD26 pKa = 4.18SFKK29 pKa = 11.13VALVTSSGNISASTTAWSGVTGEE52 pKa = 4.21VASGNGYY59 pKa = 5.63TTGGEE64 pKa = 4.43SVTLSLTGTTSVAVTFASNPVWTASGSGIAAKK96 pKa = 9.28WAVIYY101 pKa = 10.04EE102 pKa = 4.11VGGNVLAYY110 pKa = 10.43ALLNSDD116 pKa = 4.33GSTSTTTDD124 pKa = 2.97GNTLTVNSSGANYY137 pKa = 10.03IFTLAA142 pKa = 4.06

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5R769|V5R769_9CAUD Uncharacterized protein OS=Mycobacterium phage Bruin OX=1391179 GN=4 PE=4 SV=1
MM1 pKa = 7.3TSASAVSRR9 pKa = 11.84ALNRR13 pKa = 11.84AGVITATTGWNRR25 pKa = 11.84YY26 pKa = 7.52GVFVHH31 pKa = 6.1RR32 pKa = 11.84HH33 pKa = 3.79VDD35 pKa = 3.34GAQININGRR44 pKa = 11.84TDD46 pKa = 3.4EE47 pKa = 4.5EE48 pKa = 4.5VEE50 pKa = 4.13KK51 pKa = 10.66MIDD54 pKa = 3.23RR55 pKa = 11.84VTSEE59 pKa = 5.17LSAQGYY65 pKa = 8.74RR66 pKa = 11.84VNQANARR73 pKa = 11.84TLIVRR78 pKa = 11.84KK79 pKa = 10.03SSSNKK84 pKa = 9.25RR85 pKa = 3.36

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

141

0

141

23286

32

1577

165.1

18.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.139 ± 0.4

1.267 ± 0.144

6.386 ± 0.231

6.588 ± 0.24

2.959 ± 0.156

8.756 ± 0.499

2.504 ± 0.161

4.475 ± 0.147

4.531 ± 0.226

8.121 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.113

3.427 ± 0.136

5.304 ± 0.193

3.281 ± 0.203

5.926 ± 0.304

5.634 ± 0.232

6.257 ± 0.274

7.094 ± 0.251

1.92 ± 0.131

3.053 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski