Hubei tombus-like virus 25

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGV5|A0A1L3KGV5_9VIRU Capsid protein OS=Hubei tombus-like virus 25 OX=1923272 PE=3 SV=1
MM1 pKa = 7.77FDD3 pKa = 3.7VLVDD7 pKa = 3.54NCAVDD12 pKa = 4.32EE13 pKa = 4.9DD14 pKa = 5.06PNDD17 pKa = 3.64VMNLPGVAVHH27 pKa = 6.58MDD29 pKa = 3.33AEE31 pKa = 4.22NAANYY36 pKa = 9.01INSIIEE42 pKa = 3.98PGAKK46 pKa = 9.61RR47 pKa = 11.84MGKK50 pKa = 9.72NKK52 pKa = 9.87IEE54 pKa = 4.08GLNAVSVGKK63 pKa = 10.46RR64 pKa = 11.84KK65 pKa = 9.54EE66 pKa = 4.04VVDD69 pKa = 4.06AVCGIFEE76 pKa = 5.3DD77 pKa = 4.11SANDD81 pKa = 2.98IDD83 pKa = 5.71FGIKK87 pKa = 10.54LMTSGAKK94 pKa = 10.01ARR96 pKa = 11.84IAEE99 pKa = 4.24AVNVAMDD106 pKa = 3.92EE107 pKa = 4.32AGGPAMVPVVKK118 pKa = 10.09EE119 pKa = 4.22VYY121 pKa = 8.11TQASVGCEE129 pKa = 3.92TGVGTDD135 pKa = 3.05DD136 pKa = 3.25VTRR139 pKa = 11.84PRR141 pKa = 11.84RR142 pKa = 11.84AFRR145 pKa = 11.84VQKK148 pKa = 10.56RR149 pKa = 11.84RR150 pKa = 11.84DD151 pKa = 3.32FRR153 pKa = 11.84SYY155 pKa = 11.16RR156 pKa = 11.84NAGCIEE162 pKa = 4.09ADD164 pKa = 3.47EE165 pKa = 4.29TLTYY169 pKa = 10.62YY170 pKa = 10.26LKK172 pKa = 10.63CKK174 pKa = 10.56YY175 pKa = 10.03FMKK178 pKa = 10.39PRR180 pKa = 11.84DD181 pKa = 3.96SGTLHH186 pKa = 6.44QMVSDD191 pKa = 4.24ARR193 pKa = 11.84VQMMKK198 pKa = 10.2SGHH201 pKa = 5.39TLDD204 pKa = 4.93SEE206 pKa = 4.42VDD208 pKa = 3.51YY209 pKa = 11.64EE210 pKa = 4.3MMTRR214 pKa = 11.84AVMAAFLVDD223 pKa = 3.37AAEE226 pKa = 4.1LEE228 pKa = 4.19FRR230 pKa = 11.84QRR232 pKa = 11.84LKK234 pKa = 11.23NYY236 pKa = 9.81DD237 pKa = 3.57AFDD240 pKa = 3.88AATKK244 pKa = 8.82ITEE247 pKa = 4.22AANGNLGRR255 pKa = 11.84IDD257 pKa = 4.32LLGARR262 pKa = 11.84AGQGSILGKK271 pKa = 9.25MLPSLDD277 pKa = 4.22LDD279 pKa = 4.0TPKK282 pKa = 10.75VV283 pKa = 3.44

Molecular weight: 30.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGV5|A0A1L3KGV5_9VIRU Capsid protein OS=Hubei tombus-like virus 25 OX=1923272 PE=3 SV=1
MM1 pKa = 7.38NVTIQQKK8 pKa = 10.4KK9 pKa = 10.54GSTSKK14 pKa = 10.8NKK16 pKa = 9.29QRR18 pKa = 11.84SKK20 pKa = 11.0KK21 pKa = 9.62KK22 pKa = 10.28RR23 pKa = 11.84NNKK26 pKa = 7.25TSRR29 pKa = 11.84NPLSAKK35 pKa = 9.85ASKK38 pKa = 8.94GTAKK42 pKa = 10.82ANTAPVSINVTTKK55 pKa = 10.66KK56 pKa = 10.41GIPAIKK62 pKa = 8.91STAGGITVTHH72 pKa = 6.76KK73 pKa = 10.8EE74 pKa = 4.14YY75 pKa = 10.57IGDD78 pKa = 3.8FTSNGASFALNNYY91 pKa = 8.05NVNPGLSGTFPWLSAIANRR110 pKa = 11.84YY111 pKa = 7.82EE112 pKa = 4.18SYY114 pKa = 10.94LIDD117 pKa = 4.53DD118 pKa = 3.86LHH120 pKa = 7.48FIYY123 pKa = 10.67EE124 pKa = 4.66PICPTTTPGSILMAMDD140 pKa = 3.95YY141 pKa = 10.8DD142 pKa = 4.35AADD145 pKa = 3.6SAPSNKK151 pKa = 7.28VTIMAYY157 pKa = 9.8SSAVRR162 pKa = 11.84TSPWNRR168 pKa = 11.84TVFAARR174 pKa = 11.84RR175 pKa = 11.84SDD177 pKa = 3.23LHH179 pKa = 7.32KK180 pKa = 10.8FGVQRR185 pKa = 11.84YY186 pKa = 6.83VRR188 pKa = 11.84STVPPTNTDD197 pKa = 2.57VKK199 pKa = 10.02TYY201 pKa = 10.94DD202 pKa = 3.09VGNFFLASQGTPAGPTPLGEE222 pKa = 4.7LYY224 pKa = 10.73VSYY227 pKa = 9.78TIRR230 pKa = 11.84FLTPQVGTGILAPSTVMAAEE250 pKa = 4.16SQSTILRR257 pKa = 11.84IPAGVAAVNLSGEE270 pKa = 4.24YY271 pKa = 9.71RR272 pKa = 11.84GSGVNPLAWLEE283 pKa = 3.95PLAATTPDD291 pKa = 3.69PVLLLNLNNANNMLLSFRR309 pKa = 11.84AEE311 pKa = 4.15NGWGGSAAGWNPLAFFQNMRR331 pKa = 11.84MGTSVTDD338 pKa = 3.5IGVSWAVKK346 pKa = 9.56QLAFGFAEE354 pKa = 4.26KK355 pKa = 10.53NDD357 pKa = 4.17PNSLFQTYY365 pKa = 10.18LIQPTMDD372 pKa = 4.29ALSKK376 pKa = 9.92SQGGIFPLILKK387 pKa = 10.24RR388 pKa = 11.84PATGTYY394 pKa = 9.69QMAVALMPVAANYY407 pKa = 10.08SGNIPQITQMITDD420 pKa = 3.71WAFPQLYY427 pKa = 9.66IPTFNASLLTKK438 pKa = 10.42AGMRR442 pKa = 11.84ASFTDD447 pKa = 3.43TLNTIHH453 pKa = 7.43RR454 pKa = 11.84ADD456 pKa = 3.99PQDD459 pKa = 3.88TVSMIDD465 pKa = 3.61DD466 pKa = 4.86LEE468 pKa = 4.62TKK470 pKa = 10.49VKK472 pKa = 9.98TKK474 pKa = 10.81LKK476 pKa = 9.06WW477 pKa = 3.16

Molecular weight: 51.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1318

283

558

439.3

48.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.877 ± 1.341

1.517 ± 0.561

5.539 ± 0.879

4.628 ± 0.992

3.718 ± 0.144

6.525 ± 0.515

1.366 ± 0.306

5.615 ± 0.479

5.918 ± 0.106

8.118 ± 0.595

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.338 ± 0.539

5.463 ± 0.595

4.704 ± 0.769

3.414 ± 0.375

5.842 ± 0.871

6.98 ± 0.725

6.904 ± 1.131

6.146 ± 0.966

1.442 ± 0.405

3.945 ± 0.551
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski