Vibrio phage P23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F2R1|A0A3G8F2R1_9CAUD Uncharacterized protein OS=Vibrio phage P23 OX=2488664 PE=4 SV=1
MM1 pKa = 7.67TLQQYY6 pKa = 7.13EE7 pKa = 4.59VKK9 pKa = 10.79YY10 pKa = 10.76LDD12 pKa = 3.89EE13 pKa = 6.75LIDD16 pKa = 3.52NWSGIDD22 pKa = 2.98WDD24 pKa = 4.21EE25 pKa = 3.96YY26 pKa = 11.76VRR28 pKa = 11.84DD29 pKa = 4.26CYY31 pKa = 10.35QKK33 pKa = 11.29YY34 pKa = 7.89MDD36 pKa = 5.02SMGEE40 pKa = 3.77QKK42 pKa = 10.14IAEE45 pKa = 4.32WEE47 pKa = 4.01NSQQ50 pKa = 4.56

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F2P1|A0A3G8F2P1_9CAUD Coil containing protein OS=Vibrio phage P23 OX=2488664 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.24SNKK5 pKa = 8.71VRR7 pKa = 11.84MEE9 pKa = 3.78INRR12 pKa = 11.84NHH14 pKa = 6.57HH15 pKa = 5.97EE16 pKa = 4.11WFKK19 pKa = 11.31SQMGNTNNEE28 pKa = 3.5RR29 pKa = 11.84MGCIRR34 pKa = 11.84NKK36 pKa = 10.13IEE38 pKa = 3.85THH40 pKa = 4.78NQTRR44 pKa = 11.84TSLLFWHH51 pKa = 6.68GQCTDD56 pKa = 3.41YY57 pKa = 11.81ANQNSTLKK65 pKa = 10.64RR66 pKa = 11.84LLALSITVSIISVGYY81 pKa = 9.23LAYY84 pKa = 10.48AQGWFLL90 pKa = 4.48

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12171

29

1057

169.0

18.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.723 ± 0.542

1.471 ± 0.193

6.31 ± 0.187

7.033 ± 0.295

4.133 ± 0.193

6.902 ± 0.365

1.693 ± 0.2

6.105 ± 0.208

7.362 ± 0.338

7.888 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.206

5.357 ± 0.221

3.073 ± 0.209

3.952 ± 0.312

4.199 ± 0.247

6.746 ± 0.244

5.686 ± 0.361

6.327 ± 0.226

1.421 ± 0.158

3.648 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski