Amino acid dipepetide frequency for Phage NC-G

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.916AlaAla: 5.916 ± 0.419
0.393AlaCys: 0.393 ± 0.103
4.044AlaAsp: 4.044 ± 0.258
4.999AlaGlu: 4.999 ± 0.426
2.397AlaPhe: 2.397 ± 0.224
4.943AlaGly: 4.943 ± 0.411
1.254AlaHis: 1.254 ± 0.135
4.437AlaIle: 4.437 ± 0.325
4.962AlaLys: 4.962 ± 0.321
6.553AlaLeu: 6.553 ± 0.385
1.76AlaMet: 1.76 ± 0.204
3.183AlaAsn: 3.183 ± 0.252
2.808AlaPro: 2.808 ± 0.27
2.546AlaGln: 2.546 ± 0.227
2.94AlaArg: 2.94 ± 0.214
4.681AlaSer: 4.681 ± 0.347
3.932AlaThr: 3.932 ± 0.411
4.98AlaVal: 4.98 ± 0.339
0.992AlaTrp: 0.992 ± 0.126
3.052AlaTyr: 3.052 ± 0.243
0.0AlaXaa: 0.0 ± 0.0
Cys
0.73CysAla: 0.73 ± 0.122
0.112CysCys: 0.112 ± 0.056
0.693CysAsp: 0.693 ± 0.122
0.786CysGlu: 0.786 ± 0.124
0.468CysPhe: 0.468 ± 0.111
0.768CysGly: 0.768 ± 0.119
0.243CysHis: 0.243 ± 0.075
0.599CysIle: 0.599 ± 0.112
0.73CysLys: 0.73 ± 0.105
0.674CysLeu: 0.674 ± 0.112
0.506CysMet: 0.506 ± 0.091
0.356CysAsn: 0.356 ± 0.08
0.487CysPro: 0.487 ± 0.118
0.243CysGln: 0.243 ± 0.061
0.524CysArg: 0.524 ± 0.097
0.655CysSer: 0.655 ± 0.121
0.449CysThr: 0.449 ± 0.069
0.749CysVal: 0.749 ± 0.119
0.094CysTrp: 0.094 ± 0.041
0.374CysTyr: 0.374 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
4.231AspAla: 4.231 ± 0.268
0.749AspCys: 0.749 ± 0.109
4.288AspAsp: 4.288 ± 0.286
4.681AspGlu: 4.681 ± 0.308
3.351AspPhe: 3.351 ± 0.298
4.905AspGly: 4.905 ± 0.327
0.805AspHis: 0.805 ± 0.132
5.111AspIle: 5.111 ± 0.339
4.737AspLys: 4.737 ± 0.31
5.093AspLeu: 5.093 ± 0.264
1.554AspMet: 1.554 ± 0.173
3.071AspAsn: 3.071 ± 0.187
2.191AspPro: 2.191 ± 0.234
1.835AspGln: 1.835 ± 0.202
2.322AspArg: 2.322 ± 0.209
3.314AspSer: 3.314 ± 0.24
3.351AspThr: 3.351 ± 0.322
4.175AspVal: 4.175 ± 0.249
1.236AspTrp: 1.236 ± 0.136
3.277AspTyr: 3.277 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
5.636GluAla: 5.636 ± 0.401
0.843GluCys: 0.843 ± 0.131
4.194GluAsp: 4.194 ± 0.26
4.887GluGlu: 4.887 ± 0.367
3.595GluPhe: 3.595 ± 0.321
3.857GluGly: 3.857 ± 0.3
1.311GluHis: 1.311 ± 0.146
5.804GluIle: 5.804 ± 0.351
4.4GluLys: 4.4 ± 0.314
6.815GluLeu: 6.815 ± 0.304
2.134GluMet: 2.134 ± 0.189
3.876GluAsn: 3.876 ± 0.235
2.153GluPro: 2.153 ± 0.159
2.471GluGln: 2.471 ± 0.231
2.584GluArg: 2.584 ± 0.232
3.82GluSer: 3.82 ± 0.283
4.288GluThr: 4.288 ± 0.297
5.28GluVal: 5.28 ± 0.299
1.03GluTrp: 1.03 ± 0.123
3.445GluTyr: 3.445 ± 0.277
0.0GluXaa: 0.0 ± 0.0
Phe
2.94PheAla: 2.94 ± 0.228
0.412PheCys: 0.412 ± 0.1
3.258PheAsp: 3.258 ± 0.255
3.351PheGlu: 3.351 ± 0.277
1.498PhePhe: 1.498 ± 0.161
3.127PheGly: 3.127 ± 0.22
0.618PheHis: 0.618 ± 0.107
2.996PheIle: 2.996 ± 0.229
4.157PheLys: 4.157 ± 0.315
2.265PheLeu: 2.265 ± 0.209
1.273PheMet: 1.273 ± 0.171
2.64PheAsn: 2.64 ± 0.232
1.123PhePro: 1.123 ± 0.137
1.479PheGln: 1.479 ± 0.18
1.947PheArg: 1.947 ± 0.163
2.715PheSer: 2.715 ± 0.239
2.209PheThr: 2.209 ± 0.187
3.014PheVal: 3.014 ± 0.212
0.674PheTrp: 0.674 ± 0.114
1.779PheTyr: 1.779 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
3.501GlyAla: 3.501 ± 0.293
0.786GlyCys: 0.786 ± 0.119
4.437GlyAsp: 4.437 ± 0.303
4.119GlyGlu: 4.119 ± 0.27
2.603GlyPhe: 2.603 ± 0.209
4.007GlyGly: 4.007 ± 0.613
0.955GlyHis: 0.955 ± 0.145
4.288GlyIle: 4.288 ± 0.303
4.25GlyLys: 4.25 ± 0.291
5.654GlyLeu: 5.654 ± 0.33
1.854GlyMet: 1.854 ± 0.214
3.389GlyAsn: 3.389 ± 0.339
2.041GlyPro: 2.041 ± 0.181
2.153GlyGln: 2.153 ± 0.231
2.64GlyArg: 2.64 ± 0.228
3.913GlySer: 3.913 ± 0.3
4.288GlyThr: 4.288 ± 0.383
3.894GlyVal: 3.894 ± 0.292
1.123GlyTrp: 1.123 ± 0.137
2.883GlyTyr: 2.883 ± 0.262
0.0GlyXaa: 0.0 ± 0.0
His
0.936HisAla: 0.936 ± 0.135
0.243HisCys: 0.243 ± 0.07
0.917HisAsp: 0.917 ± 0.12
1.142HisGlu: 1.142 ± 0.15
0.861HisPhe: 0.861 ± 0.119
1.123HisGly: 1.123 ± 0.142
0.412HisHis: 0.412 ± 0.082
1.217HisIle: 1.217 ± 0.16
1.386HisLys: 1.386 ± 0.183
1.367HisLeu: 1.367 ± 0.164
0.262HisMet: 0.262 ± 0.074
0.88HisAsn: 0.88 ± 0.124
1.067HisPro: 1.067 ± 0.135
0.524HisGln: 0.524 ± 0.091
0.655HisArg: 0.655 ± 0.117
1.067HisSer: 1.067 ± 0.12
0.992HisThr: 0.992 ± 0.139
1.03HisVal: 1.03 ± 0.136
0.169HisTrp: 0.169 ± 0.054
0.674HisTyr: 0.674 ± 0.125
0.0HisXaa: 0.0 ± 0.0
Ile
4.887IleAla: 4.887 ± 0.327
0.562IleCys: 0.562 ± 0.101
5.13IleAsp: 5.13 ± 0.313
5.186IleGlu: 5.186 ± 0.356
2.265IlePhe: 2.265 ± 0.192
3.22IleGly: 3.22 ± 0.216
1.329IleHis: 1.329 ± 0.154
4.082IleIle: 4.082 ± 0.246
7.021IleLys: 7.021 ± 0.418
4.25IleLeu: 4.25 ± 0.289
1.891IleMet: 1.891 ± 0.182
4.063IleAsn: 4.063 ± 0.311
2.752IlePro: 2.752 ± 0.225
2.546IleGln: 2.546 ± 0.238
3.351IleArg: 3.351 ± 0.254
3.894IleSer: 3.894 ± 0.287
4.568IleThr: 4.568 ± 0.261
4.288IleVal: 4.288 ± 0.291
0.599IleTrp: 0.599 ± 0.102
2.172IleTyr: 2.172 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
5.991LysAla: 5.991 ± 0.351
0.824LysCys: 0.824 ± 0.113
4.475LysAsp: 4.475 ± 0.308
5.879LysGlu: 5.879 ± 0.369
3.258LysPhe: 3.258 ± 0.228
4.568LysGly: 4.568 ± 0.295
1.591LysHis: 1.591 ± 0.175
4.812LysIle: 4.812 ± 0.244
4.699LysLys: 4.699 ± 0.329
6.291LysLeu: 6.291 ± 0.34
2.64LysMet: 2.64 ± 0.248
3.595LysAsn: 3.595 ± 0.223
2.509LysPro: 2.509 ± 0.225
2.621LysGln: 2.621 ± 0.234
3.164LysArg: 3.164 ± 0.262
4.306LysSer: 4.306 ± 0.347
3.969LysThr: 3.969 ± 0.279
5.579LysVal: 5.579 ± 0.385
1.03LysTrp: 1.03 ± 0.124
3.22LysTyr: 3.22 ± 0.251
0.0LysXaa: 0.0 ± 0.0
Leu
5.636LeuAla: 5.636 ± 0.292
0.58LeuCys: 0.58 ± 0.11
5.299LeuAsp: 5.299 ± 0.314
5.374LeuGlu: 5.374 ± 0.35
3.445LeuPhe: 3.445 ± 0.306
4.231LeuGly: 4.231 ± 0.299
1.123LeuHis: 1.123 ± 0.124
4.868LeuIle: 4.868 ± 0.333
6.235LeuLys: 6.235 ± 0.342
5.392LeuLeu: 5.392 ± 0.408
2.34LeuMet: 2.34 ± 0.245
4.681LeuAsn: 4.681 ± 0.28
2.958LeuPro: 2.958 ± 0.232
2.64LeuGln: 2.64 ± 0.231
3.351LeuArg: 3.351 ± 0.232
5.149LeuSer: 5.149 ± 0.296
4.625LeuThr: 4.625 ± 0.363
4.831LeuVal: 4.831 ± 0.297
0.749LeuTrp: 0.749 ± 0.115
3.426LeuTyr: 3.426 ± 0.257
0.0LeuXaa: 0.0 ± 0.0
Met
2.415MetAla: 2.415 ± 0.223
0.412MetCys: 0.412 ± 0.086
1.928MetAsp: 1.928 ± 0.21
1.573MetGlu: 1.573 ± 0.176
1.329MetPhe: 1.329 ± 0.155
1.404MetGly: 1.404 ± 0.165
0.318MetHis: 0.318 ± 0.07
1.723MetIle: 1.723 ± 0.193
2.434MetLys: 2.434 ± 0.217
1.779MetLeu: 1.779 ± 0.196
0.768MetMet: 0.768 ± 0.121
1.779MetAsn: 1.779 ± 0.152
0.824MetPro: 0.824 ± 0.133
0.974MetGln: 0.974 ± 0.133
1.142MetArg: 1.142 ± 0.15
2.116MetSer: 2.116 ± 0.207
1.779MetThr: 1.779 ± 0.174
1.797MetVal: 1.797 ± 0.178
0.318MetTrp: 0.318 ± 0.077
1.067MetTyr: 1.067 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.445AsnAla: 3.445 ± 0.269
0.506AsnCys: 0.506 ± 0.11
3.183AsnAsp: 3.183 ± 0.234
3.745AsnGlu: 3.745 ± 0.254
2.715AsnPhe: 2.715 ± 0.209
4.269AsnGly: 4.269 ± 0.333
1.048AsnHis: 1.048 ± 0.153
3.745AsnIle: 3.745 ± 0.213
3.651AsnLys: 3.651 ± 0.234
3.52AsnLeu: 3.52 ± 0.278
1.479AsnMet: 1.479 ± 0.166
3.089AsnAsn: 3.089 ± 0.275
2.528AsnPro: 2.528 ± 0.231
1.872AsnGln: 1.872 ± 0.193
2.116AsnArg: 2.116 ± 0.179
3.632AsnSer: 3.632 ± 0.28
3.033AsnThr: 3.033 ± 0.244
3.145AsnVal: 3.145 ± 0.307
0.599AsnTrp: 0.599 ± 0.109
2.003AsnTyr: 2.003 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
2.471ProAla: 2.471 ± 0.208
0.337ProCys: 0.337 ± 0.08
2.528ProAsp: 2.528 ± 0.213
3.37ProGlu: 3.37 ± 0.288
1.423ProPhe: 1.423 ± 0.16
2.64ProGly: 2.64 ± 0.212
0.618ProHis: 0.618 ± 0.102
2.415ProIle: 2.415 ± 0.241
2.546ProLys: 2.546 ± 0.233
2.808ProLeu: 2.808 ± 0.216
0.637ProMet: 0.637 ± 0.122
1.854ProAsn: 1.854 ± 0.173
0.917ProPro: 0.917 ± 0.146
1.03ProGln: 1.03 ± 0.132
1.348ProArg: 1.348 ± 0.175
2.359ProSer: 2.359 ± 0.22
2.153ProThr: 2.153 ± 0.201
2.827ProVal: 2.827 ± 0.233
0.599ProTrp: 0.599 ± 0.113
1.554ProTyr: 1.554 ± 0.161
0.0ProXaa: 0.0 ± 0.0
Gln
2.921GlnAla: 2.921 ± 0.261
0.318GlnCys: 0.318 ± 0.084
1.891GlnAsp: 1.891 ± 0.183
2.303GlnGlu: 2.303 ± 0.212
1.591GlnPhe: 1.591 ± 0.167
1.966GlnGly: 1.966 ± 0.166
0.431GlnHis: 0.431 ± 0.082
2.565GlnIle: 2.565 ± 0.205
2.041GlnLys: 2.041 ± 0.19
2.958GlnLeu: 2.958 ± 0.236
0.992GlnMet: 0.992 ± 0.134
1.648GlnAsn: 1.648 ± 0.157
0.974GlnPro: 0.974 ± 0.126
1.067GlnGln: 1.067 ± 0.169
1.985GlnArg: 1.985 ± 0.163
2.041GlnSer: 2.041 ± 0.198
2.134GlnThr: 2.134 ± 0.234
2.34GlnVal: 2.34 ± 0.2
0.917GlnTrp: 0.917 ± 0.144
1.591GlnTyr: 1.591 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
2.621ArgAla: 2.621 ± 0.244
0.449ArgCys: 0.449 ± 0.086
2.509ArgAsp: 2.509 ± 0.209
2.958ArgGlu: 2.958 ± 0.226
1.966ArgPhe: 1.966 ± 0.188
2.49ArgGly: 2.49 ± 0.236
0.749ArgHis: 0.749 ± 0.118
3.501ArgIle: 3.501 ± 0.228
3.108ArgLys: 3.108 ± 0.271
3.614ArgLeu: 3.614 ± 0.339
1.311ArgMet: 1.311 ± 0.155
2.284ArgAsn: 2.284 ± 0.205
1.273ArgPro: 1.273 ± 0.143
1.816ArgGln: 1.816 ± 0.181
2.191ArgArg: 2.191 ± 0.199
2.621ArgSer: 2.621 ± 0.23
2.228ArgThr: 2.228 ± 0.19
2.752ArgVal: 2.752 ± 0.284
0.599ArgTrp: 0.599 ± 0.108
1.704ArgTyr: 1.704 ± 0.209
0.0ArgXaa: 0.0 ± 0.0
Ser
3.408SerAla: 3.408 ± 0.28
0.73SerCys: 0.73 ± 0.124
4.082SerAsp: 4.082 ± 0.26
4.512SerGlu: 4.512 ± 0.317
2.621SerPhe: 2.621 ± 0.201
4.325SerGly: 4.325 ± 0.327
1.142SerHis: 1.142 ± 0.155
4.063SerIle: 4.063 ± 0.249
4.943SerLys: 4.943 ± 0.303
4.905SerLeu: 4.905 ± 0.314
1.685SerMet: 1.685 ± 0.172
2.734SerAsn: 2.734 ± 0.302
2.378SerPro: 2.378 ± 0.258
2.116SerGln: 2.116 ± 0.183
2.715SerArg: 2.715 ± 0.258
4.362SerSer: 4.362 ± 0.326
3.501SerThr: 3.501 ± 0.272
4.063SerVal: 4.063 ± 0.252
0.917SerTrp: 0.917 ± 0.16
2.64SerTyr: 2.64 ± 0.23
0.0SerXaa: 0.0 ± 0.0
Thr
4.625ThrAla: 4.625 ± 0.374
0.431ThrCys: 0.431 ± 0.091
3.127ThrAsp: 3.127 ± 0.239
4.362ThrGlu: 4.362 ± 0.351
2.696ThrPhe: 2.696 ± 0.209
4.344ThrGly: 4.344 ± 0.303
0.768ThrHis: 0.768 ± 0.117
3.726ThrIle: 3.726 ± 0.274
3.951ThrLys: 3.951 ± 0.301
4.4ThrLeu: 4.4 ± 0.363
1.198ThrMet: 1.198 ± 0.16
2.846ThrAsn: 2.846 ± 0.195
2.659ThrPro: 2.659 ± 0.201
2.022ThrGln: 2.022 ± 0.234
2.921ThrArg: 2.921 ± 0.295
3.614ThrSer: 3.614 ± 0.322
3.258ThrThr: 3.258 ± 0.33
4.231ThrVal: 4.231 ± 0.292
0.637ThrTrp: 0.637 ± 0.105
2.134ThrTyr: 2.134 ± 0.204
0.0ThrXaa: 0.0 ± 0.0
Val
4.756ValAla: 4.756 ± 0.305
0.917ValCys: 0.917 ± 0.129
4.587ValAsp: 4.587 ± 0.274
5.467ValGlu: 5.467 ± 0.356
2.921ValPhe: 2.921 ± 0.265
3.445ValGly: 3.445 ± 0.301
1.03ValHis: 1.03 ± 0.144
4.4ValIle: 4.4 ± 0.287
5.28ValLys: 5.28 ± 0.325
4.55ValLeu: 4.55 ± 0.291
1.947ValMet: 1.947 ± 0.193
3.876ValAsn: 3.876 ± 0.287
2.752ValPro: 2.752 ± 0.223
2.49ValGln: 2.49 ± 0.201
2.734ValArg: 2.734 ± 0.232
3.969ValSer: 3.969 ± 0.285
3.894ValThr: 3.894 ± 0.322
4.4ValVal: 4.4 ± 0.279
0.899ValTrp: 0.899 ± 0.12
3.145ValTyr: 3.145 ± 0.234
0.0ValXaa: 0.0 ± 0.0
Trp
0.768TrpAla: 0.768 ± 0.103
0.131TrpCys: 0.131 ± 0.05
0.768TrpAsp: 0.768 ± 0.123
0.786TrpGlu: 0.786 ± 0.107
0.637TrpPhe: 0.637 ± 0.105
0.506TrpGly: 0.506 ± 0.129
0.337TrpHis: 0.337 ± 0.078
0.805TrpIle: 0.805 ± 0.123
1.535TrpLys: 1.535 ± 0.161
1.161TrpLeu: 1.161 ± 0.122
0.524TrpMet: 0.524 ± 0.11
0.861TrpAsn: 0.861 ± 0.11
0.449TrpPro: 0.449 ± 0.101
0.449TrpGln: 0.449 ± 0.101
0.412TrpArg: 0.412 ± 0.088
0.768TrpSer: 0.768 ± 0.137
0.861TrpThr: 0.861 ± 0.121
1.217TrpVal: 1.217 ± 0.128
0.243TrpTrp: 0.243 ± 0.065
0.824TrpTyr: 0.824 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.94TyrAla: 2.94 ± 0.262
0.506TyrCys: 0.506 ± 0.084
2.94TyrAsp: 2.94 ± 0.218
2.94TyrGlu: 2.94 ± 0.237
1.854TyrPhe: 1.854 ± 0.196
2.64TyrGly: 2.64 ± 0.22
0.843TyrHis: 0.843 ± 0.114
2.902TyrIle: 2.902 ± 0.254
2.958TyrLys: 2.958 ± 0.252
2.883TyrLeu: 2.883 ± 0.27
1.105TyrMet: 1.105 ± 0.137
2.621TyrAsn: 2.621 ± 0.198
1.591TyrPro: 1.591 ± 0.173
1.779TyrGln: 1.779 ± 0.176
1.704TyrArg: 1.704 ± 0.179
2.846TyrSer: 2.846 ± 0.235
2.434TyrThr: 2.434 ± 0.217
2.865TyrVal: 2.865 ± 0.245
0.618TyrTrp: 0.618 ± 0.111
1.779TyrTyr: 1.779 ± 0.163
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 275 proteins (53411 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski