Homo sapiens (Human)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2Z311|F2Z311_HUMAN Isoform of Q86TG1 Transmembrane protein 150A OS=Homo sapiens OX=9606 GN=TMEM150A PE=4 SV=1
MM1 pKa = 7.5SEE3 pKa = 4.04KK4 pKa = 10.31KK5 pKa = 10.32QPVDD9 pKa = 3.98LGLLEE14 pKa = 4.72EE15 pKa = 5.08DD16 pKa = 4.95DD17 pKa = 4.2EE18 pKa = 5.16FEE20 pKa = 4.5EE21 pKa = 6.21FPAEE25 pKa = 3.92DD26 pKa = 3.4WAGLDD31 pKa = 3.67EE32 pKa = 6.8DD33 pKa = 4.65EE34 pKa = 5.59DD35 pKa = 3.98AHH37 pKa = 7.46VWEE40 pKa = 5.76DD41 pKa = 3.11NWDD44 pKa = 3.91DD45 pKa = 5.56DD46 pKa = 4.79NVEE49 pKa = 4.84DD50 pKa = 5.62DD51 pKa = 4.76FSNQLRR57 pKa = 11.84ATVLLMILVCEE68 pKa = 4.39TPYY71 pKa = 11.06GCYY74 pKa = 9.94VLHH77 pKa = 6.36QKK79 pKa = 10.5GRR81 pKa = 11.84MCSAFLCCC89 pKa = 6.08

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9UKM7|MA1B1_HUMAN Endoplasmic reticulum mannosyl-oligosaccharide 1 2-alpha-mannosidase OS=Homo sapiens OX=9606 GN=MAN1B1 PE=1 SV=2
MM1 pKa = 7.76RR2 pKa = 11.84RR3 pKa = 11.84PSTASLTRR11 pKa = 11.84TPSRR15 pKa = 11.84ASPTRR20 pKa = 11.84MPSRR24 pKa = 11.84ASLKK28 pKa = 7.11MTPFRR33 pKa = 11.84ASLTKK38 pKa = 9.87MEE40 pKa = 4.28STALLRR46 pKa = 11.84TLPRR50 pKa = 11.84ASLMRR55 pKa = 11.84TPTRR59 pKa = 11.84ASLMRR64 pKa = 11.84TPPRR68 pKa = 11.84ASPTRR73 pKa = 11.84KK74 pKa = 9.24PPRR77 pKa = 11.84ASPRR81 pKa = 11.84TPSRR85 pKa = 11.84ASPTRR90 pKa = 11.84RR91 pKa = 11.84LPRR94 pKa = 11.84ASPMGSPHH102 pKa = 6.87RR103 pKa = 11.84ASPMRR108 pKa = 11.84TPPRR112 pKa = 11.84ASPTGTPSTASPTGTPSSASPTGTPPRR139 pKa = 11.84ASPTGTPPRR148 pKa = 11.84AWATRR153 pKa = 11.84SPSTASLTRR162 pKa = 11.84TPSRR166 pKa = 11.84ASLTRR171 pKa = 11.84WPPRR175 pKa = 11.84ASPTRR180 pKa = 11.84TPPRR184 pKa = 11.84EE185 pKa = 4.12SPRR188 pKa = 11.84MSHH191 pKa = 6.34RR192 pKa = 11.84ASPTRR197 pKa = 11.84TPPRR201 pKa = 11.84ASPTRR206 pKa = 11.84RR207 pKa = 11.84PPRR210 pKa = 11.84ASPTRR215 pKa = 11.84TPPRR219 pKa = 11.84EE220 pKa = 4.02SLRR223 pKa = 11.84TSHH226 pKa = 6.8RR227 pKa = 11.84ASPTRR232 pKa = 11.84MPPRR236 pKa = 11.84ASPTRR241 pKa = 11.84RR242 pKa = 11.84PPRR245 pKa = 11.84ASPTGSPPRR254 pKa = 11.84ASPMTPPRR262 pKa = 11.84ASPRR266 pKa = 11.84TPPRR270 pKa = 11.84ASPTTTPSRR279 pKa = 11.84ASLTRR284 pKa = 11.84TPSWASPTTTPSRR297 pKa = 11.84ASLMKK302 pKa = 9.87MEE304 pKa = 4.46STVSITRR311 pKa = 11.84TPPRR315 pKa = 11.84ASPTGTPSRR324 pKa = 11.84ASPTGTPSRR333 pKa = 11.84ASLTGSPSRR342 pKa = 11.84ASLTGTPSRR351 pKa = 11.84ASLIGTPSRR360 pKa = 11.84ASLIGTPSRR369 pKa = 11.84ASLTGTPPRR378 pKa = 11.84ASLTGTSSTASLTRR392 pKa = 11.84TPSRR396 pKa = 11.84ASLTRR401 pKa = 11.84TQSSSSLTRR410 pKa = 11.84TPSMASLTRR419 pKa = 11.84TPPRR423 pKa = 11.84ASLTRR428 pKa = 11.84TPPRR432 pKa = 11.84ASLTRR437 pKa = 11.84TPPRR441 pKa = 11.84ASLTRR446 pKa = 11.84TPPRR450 pKa = 11.84ASLTRR455 pKa = 11.84TPSMVSLKK463 pKa = 10.16RR464 pKa = 11.84SPSRR468 pKa = 11.84ASLTRR473 pKa = 11.84TPSRR477 pKa = 11.84ASLTMTPSRR486 pKa = 11.84ASLTRR491 pKa = 11.84TPSTASLTGTPPTASLTRR509 pKa = 11.84TPPTASLTRR518 pKa = 11.84SPPTASLTRR527 pKa = 11.84TPSTASLTRR536 pKa = 11.84MPSTASLTRR545 pKa = 11.84KK546 pKa = 10.27SNVNQQCPASTPSSEE561 pKa = 4.48VISS564 pKa = 3.92

Molecular weight: 59.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20600

79500

100100

40284240

2

35991

402.4

44.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.92 ± 0.009

2.167 ± 0.008

4.835 ± 0.006

7.207 ± 0.013

3.549 ± 0.007

6.503 ± 0.014

2.577 ± 0.006

4.346 ± 0.01

5.77 ± 0.015

9.837 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.005

3.605 ± 0.007

6.3 ± 0.013

4.81 ± 0.013

5.645 ± 0.01

8.408 ± 0.017

5.466 ± 0.021

6.019 ± 0.014

1.223 ± 0.003

2.607 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski