Nitrosomonas halophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3GY40|A0A1H3GY40_9PROT Histidine kinase OS=Nitrosomonas halophila OX=44576 GN=SAMN05421881_101721 PE=4 SV=1
MM1 pKa = 7.41TYY3 pKa = 10.69VVTEE7 pKa = 3.93SCIKK11 pKa = 10.42CKK13 pKa = 10.15YY14 pKa = 7.55TDD16 pKa = 4.08CVDD19 pKa = 3.61VCPVDD24 pKa = 4.18CFRR27 pKa = 11.84EE28 pKa = 4.9GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.64EE39 pKa = 6.0CIDD42 pKa = 3.74CTLCVAEE49 pKa = 5.08CPVEE53 pKa = 4.68AIYY56 pKa = 11.16AEE58 pKa = 4.76DD59 pKa = 5.33DD60 pKa = 3.75VPDD63 pKa = 4.17DD64 pKa = 3.45QRR66 pKa = 11.84QFIALNAEE74 pKa = 4.47LSKK77 pKa = 11.01AWDD80 pKa = 4.28PIIEE84 pKa = 4.69KK85 pKa = 10.42KK86 pKa = 10.48DD87 pKa = 3.62ALPDD91 pKa = 3.95ADD93 pKa = 4.36DD94 pKa = 3.74WANVTDD100 pKa = 5.38KK101 pKa = 11.15LDD103 pKa = 3.45KK104 pKa = 11.15LEE106 pKa = 4.03RR107 pKa = 5.16

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3QC73|A0A1H3QC73_9PROT Phosphate transport system substrate-binding protein OS=Nitrosomonas halophila OX=44576 GN=SAMN05421881_11502 PE=4 SV=1
MM1 pKa = 7.39VEE3 pKa = 4.06RR4 pKa = 11.84RR5 pKa = 11.84NSRR8 pKa = 11.84AVCSVCGMPRR18 pKa = 11.84AGYY21 pKa = 10.17DD22 pKa = 2.88RR23 pKa = 11.84LGQRR27 pKa = 11.84RR28 pKa = 11.84FEE30 pKa = 5.04FVPLWGIKK38 pKa = 9.08TYY40 pKa = 10.83LVYY43 pKa = 10.64APRR46 pKa = 11.84RR47 pKa = 11.84VDD49 pKa = 3.5CPKK52 pKa = 10.3CGVRR56 pKa = 11.84VEE58 pKa = 4.28SMPWALGKK66 pKa = 10.51RR67 pKa = 11.84PLIQAMGWFLAGWAKK82 pKa = 10.23RR83 pKa = 11.84LSWKK87 pKa = 8.24EE88 pKa = 3.46TAEE91 pKa = 4.08VFRR94 pKa = 11.84TSWEE98 pKa = 4.27SVFRR102 pKa = 11.84SAEE105 pKa = 3.87MAVQWGRR112 pKa = 11.84EE113 pKa = 3.95HH114 pKa = 7.49RR115 pKa = 11.84DD116 pKa = 3.27LSGVRR121 pKa = 11.84AA122 pKa = 3.82

Molecular weight: 14.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2974

0

2974

917619

27

2992

308.5

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.143 ± 0.049

1.051 ± 0.018

5.283 ± 0.035

5.838 ± 0.041

3.846 ± 0.03

7.157 ± 0.046

2.668 ± 0.022

6.134 ± 0.037

3.979 ± 0.04

10.966 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.021

3.345 ± 0.031

4.658 ± 0.032

4.602 ± 0.036

6.313 ± 0.039

5.748 ± 0.033

5.099 ± 0.033

6.502 ± 0.037

1.374 ± 0.022

2.764 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski