Amino acid dipepetide frequency for Acanthamoeba polyphaga moumouvirus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.273AlaAla: 1.273 ± 0.08
0.86AlaCys: 0.86 ± 0.062
1.872AlaAsp: 1.872 ± 0.103
1.397AlaGlu: 1.397 ± 0.083
1.373AlaPhe: 1.373 ± 0.078
1.408AlaGly: 1.408 ± 0.105
0.547AlaHis: 0.547 ± 0.042
3.008AlaIle: 3.008 ± 0.121
2.375AlaLys: 2.375 ± 0.106
2.674AlaLeu: 2.674 ± 0.139
0.582AlaMet: 0.582 ± 0.054
2.378AlaAsn: 2.378 ± 0.114
0.805AlaPro: 0.805 ± 0.078
1.16AlaGln: 1.16 ± 0.074
1.053AlaArg: 1.053 ± 0.053
2.34AlaSer: 2.34 ± 0.132
1.497AlaThr: 1.497 ± 0.084
1.518AlaVal: 1.518 ± 0.096
0.203AlaTrp: 0.203 ± 0.028
1.425AlaTyr: 1.425 ± 0.084
0.0AlaXaa: 0.0 ± 0.0
Cys
0.743CysAla: 0.743 ± 0.051
0.441CysCys: 0.441 ± 0.05
1.236CysAsp: 1.236 ± 0.068
1.081CysGlu: 1.081 ± 0.072
0.754CysPhe: 0.754 ± 0.049
1.273CysGly: 1.273 ± 0.108
0.516CysHis: 0.516 ± 0.043
1.883CysIle: 1.883 ± 0.126
1.452CysLys: 1.452 ± 0.086
1.487CysLeu: 1.487 ± 0.08
0.361CysMet: 0.361 ± 0.039
1.322CysAsn: 1.322 ± 0.066
0.754CysPro: 0.754 ± 0.071
0.609CysGln: 0.609 ± 0.049
0.623CysArg: 0.623 ± 0.045
1.019CysSer: 1.019 ± 0.058
0.747CysThr: 0.747 ± 0.059
0.974CysVal: 0.974 ± 0.066
0.189CysTrp: 0.189 ± 0.026
0.888CysTyr: 0.888 ± 0.056
0.0CysXaa: 0.0 ± 0.0
Asp
1.597AspAla: 1.597 ± 0.073
0.988AspCys: 0.988 ± 0.062
3.827AspAsp: 3.827 ± 0.159
4.017AspGlu: 4.017 ± 0.122
3.397AspPhe: 3.397 ± 0.113
1.969AspGly: 1.969 ± 0.082
1.064AspHis: 1.064 ± 0.057
7.558AspIle: 7.558 ± 0.143
6.99AspLys: 6.99 ± 0.59
6.023AspLeu: 6.023 ± 0.281
1.236AspMet: 1.236 ± 0.071
5.627AspAsn: 5.627 ± 0.165
2.227AspPro: 2.227 ± 0.097
1.6AspGln: 1.6 ± 0.073
1.545AspArg: 1.545 ± 0.083
3.555AspSer: 3.555 ± 0.143
2.654AspThr: 2.654 ± 0.104
3.029AspVal: 3.029 ± 0.111
0.509AspTrp: 0.509 ± 0.044
3.772AspTyr: 3.772 ± 0.128
0.0AspXaa: 0.0 ± 0.0
Glu
1.69GluAla: 1.69 ± 0.09
1.112GluCys: 1.112 ± 0.076
2.891GluAsp: 2.891 ± 0.106
3.569GluGlu: 3.569 ± 0.186
2.791GluPhe: 2.791 ± 0.101
1.6GluGly: 1.6 ± 0.084
0.94GluHis: 0.94 ± 0.052
6.474GluIle: 6.474 ± 0.158
5.627GluLys: 5.627 ± 0.218
5.035GluLeu: 5.035 ± 0.155
1.208GluMet: 1.208 ± 0.073
6.371GluAsn: 6.371 ± 0.179
1.518GluPro: 1.518 ± 0.08
1.728GluGln: 1.728 ± 0.117
1.766GluArg: 1.766 ± 0.09
3.779GluSer: 3.779 ± 0.157
2.881GluThr: 2.881 ± 0.125
2.075GluVal: 2.075 ± 0.096
0.413GluTrp: 0.413 ± 0.042
3.796GluTyr: 3.796 ± 0.138
0.0GluXaa: 0.0 ± 0.0
Phe
1.37PheAla: 1.37 ± 0.071
0.909PheCys: 0.909 ± 0.065
3.562PheAsp: 3.562 ± 0.137
2.726PheGlu: 2.726 ± 0.094
2.069PhePhe: 2.069 ± 0.097
2.946PheGly: 2.946 ± 0.167
0.823PheHis: 0.823 ± 0.051
4.781PheIle: 4.781 ± 0.151
3.872PheLys: 3.872 ± 0.148
3.569PheLeu: 3.569 ± 0.122
1.225PheMet: 1.225 ± 0.061
5.08PheAsn: 5.08 ± 0.184
1.298PhePro: 1.298 ± 0.057
1.329PheGln: 1.329 ± 0.077
1.439PheArg: 1.439 ± 0.071
2.826PheSer: 2.826 ± 0.104
2.358PheThr: 2.358 ± 0.095
2.168PheVal: 2.168 ± 0.089
0.389PheTrp: 0.389 ± 0.043
2.602PheTyr: 2.602 ± 0.103
0.0PheXaa: 0.0 ± 0.0
Gly
2.058GlyAla: 2.058 ± 0.148
1.15GlyCys: 1.15 ± 0.126
4.351GlyAsp: 4.351 ± 0.955
2.368GlyGlu: 2.368 ± 0.276
2.199GlyPhe: 2.199 ± 0.102
2.389GlyGly: 2.389 ± 0.248
1.17GlyHis: 1.17 ± 0.088
4.099GlyIle: 4.099 ± 0.171
3.507GlyLys: 3.507 ± 0.126
3.383GlyLeu: 3.383 ± 0.151
0.747GlyMet: 0.747 ± 0.093
3.5GlyAsn: 3.5 ± 0.193
1.377GlyPro: 1.377 ± 0.125
1.267GlyGln: 1.267 ± 0.081
1.273GlyArg: 1.273 ± 0.064
3.208GlySer: 3.208 ± 0.231
2.275GlyThr: 2.275 ± 0.124
1.941GlyVal: 1.941 ± 0.088
0.527GlyTrp: 0.527 ± 0.056
2.509GlyTyr: 2.509 ± 0.094
0.0GlyXaa: 0.0 ± 0.0
His
0.657HisAla: 0.657 ± 0.065
0.33HisCys: 0.33 ± 0.034
1.218HisAsp: 1.218 ± 0.077
1.084HisGlu: 1.084 ± 0.068
1.005HisPhe: 1.005 ± 0.057
0.898HisGly: 0.898 ± 0.057
0.602HisHis: 0.602 ± 0.08
2.01HisIle: 2.01 ± 0.086
1.779HisLys: 1.779 ± 0.081
2.375HisLeu: 2.375 ± 0.186
0.413HisMet: 0.413 ± 0.04
1.752HisAsn: 1.752 ± 0.092
0.737HisPro: 0.737 ± 0.05
0.547HisGln: 0.547 ± 0.037
0.578HisArg: 0.578 ± 0.045
1.022HisSer: 1.022 ± 0.051
0.761HisThr: 0.761 ± 0.059
0.833HisVal: 0.833 ± 0.059
0.189HisTrp: 0.189 ± 0.028
1.122HisTyr: 1.122 ± 0.052
0.0HisXaa: 0.0 ± 0.0
Ile
2.544IleAla: 2.544 ± 0.108
1.859IleCys: 1.859 ± 0.083
6.55IleAsp: 6.55 ± 0.16
5.713IleGlu: 5.713 ± 0.166
5.039IlePhe: 5.039 ± 0.169
3.569IleGly: 3.569 ± 0.149
2.12IleHis: 2.12 ± 0.085
11.864IleIle: 11.864 ± 0.284
10.666IleLys: 10.666 ± 0.261
8.897IleLeu: 8.897 ± 0.21
2.058IleMet: 2.058 ± 0.089
11.582IleAsn: 11.582 ± 0.306
4.251IlePro: 4.251 ± 0.196
2.688IleGln: 2.688 ± 0.103
3.067IleArg: 3.067 ± 0.116
6.746IleSer: 6.746 ± 0.17
4.843IleThr: 4.843 ± 0.137
4.468IleVal: 4.468 ± 0.137
0.785IleTrp: 0.785 ± 0.048
5.831IleTyr: 5.831 ± 0.167
0.0IleXaa: 0.0 ± 0.0
Lys
1.876LysAla: 1.876 ± 0.098
1.662LysCys: 1.662 ± 0.088
4.096LysAsp: 4.096 ± 0.139
4.509LysGlu: 4.509 ± 0.168
4.375LysPhe: 4.375 ± 0.142
4.653LysGly: 4.653 ± 1.043
1.687LysHis: 1.687 ± 0.088
10.828LysIle: 10.828 ± 0.219
9.186LysLys: 9.186 ± 0.28
7.954LysLeu: 7.954 ± 0.183
2.007LysMet: 2.007 ± 0.081
10.136LysAsn: 10.136 ± 0.248
2.375LysPro: 2.375 ± 0.092
2.406LysGln: 2.406 ± 0.092
2.444LysArg: 2.444 ± 0.125
5.652LysSer: 5.652 ± 0.197
4.471LysThr: 4.471 ± 0.162
2.843LysVal: 2.843 ± 0.113
0.781LysTrp: 0.781 ± 0.05
8.574LysTyr: 8.574 ± 0.217
0.0LysXaa: 0.0 ± 0.0
Leu
2.843LeuAla: 2.843 ± 0.104
1.263LeuCys: 1.263 ± 0.067
5.879LeuAsp: 5.879 ± 0.152
5.755LeuGlu: 5.755 ± 0.144
3.896LeuPhe: 3.896 ± 0.14
3.769LeuGly: 3.769 ± 0.292
1.442LeuHis: 1.442 ± 0.074
7.858LeuIle: 7.858 ± 0.205
7.699LeuLys: 7.699 ± 0.202
7.954LeuLeu: 7.954 ± 0.255
1.79LeuMet: 1.79 ± 0.09
7.39LeuAsn: 7.39 ± 0.213
2.929LeuPro: 2.929 ± 0.117
2.681LeuGln: 2.681 ± 0.112
2.898LeuArg: 2.898 ± 0.129
6.288LeuSer: 6.288 ± 0.158
4.072LeuThr: 4.072 ± 0.144
4.141LeuVal: 4.141 ± 0.142
0.571LeuTrp: 0.571 ± 0.057
4.147LeuTyr: 4.147 ± 0.137
0.0LeuXaa: 0.0 ± 0.0
Met
0.809MetAla: 0.809 ± 0.054
0.365MetCys: 0.365 ± 0.036
1.463MetAsp: 1.463 ± 0.072
1.339MetGlu: 1.339 ± 0.061
0.809MetPhe: 0.809 ± 0.053
0.891MetGly: 0.891 ± 0.082
0.416MetHis: 0.416 ± 0.041
1.945MetIle: 1.945 ± 0.09
1.49MetLys: 1.49 ± 0.089
1.504MetLeu: 1.504 ± 0.067
0.458MetMet: 0.458 ± 0.043
2.051MetAsn: 2.051 ± 0.153
0.609MetPro: 0.609 ± 0.044
0.52MetGln: 0.52 ± 0.049
0.644MetArg: 0.644 ± 0.048
1.924MetSer: 1.924 ± 0.076
1.229MetThr: 1.229 ± 0.071
0.847MetVal: 0.847 ± 0.059
0.148MetTrp: 0.148 ± 0.024
1.026MetTyr: 1.026 ± 0.064
0.0MetXaa: 0.0 ± 0.0
Asn
2.289AsnAla: 2.289 ± 0.107
1.391AsnCys: 1.391 ± 0.07
5.6AsnAsp: 5.6 ± 0.143
4.874AsnGlu: 4.874 ± 0.156
4.426AsnPhe: 4.426 ± 0.144
4.388AsnGly: 4.388 ± 0.178
1.962AsnHis: 1.962 ± 0.084
12.604AsnIle: 12.604 ± 0.271
9.654AsnLys: 9.654 ± 0.219
7.865AsnLeu: 7.865 ± 0.201
2.461AsnMet: 2.461 ± 0.144
12.446AsnAsn: 12.446 ± 0.519
3.197AsnPro: 3.197 ± 0.141
3.16AsnGln: 3.16 ± 0.17
2.482AsnArg: 2.482 ± 0.127
5.548AsnSer: 5.548 ± 0.191
4.702AsnThr: 4.702 ± 0.181
3.796AsnVal: 3.796 ± 0.124
0.781AsnTrp: 0.781 ± 0.065
5.631AsnTyr: 5.631 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
0.905ProAla: 0.905 ± 0.071
0.644ProCys: 0.644 ± 0.133
2.282ProAsp: 2.282 ± 0.095
2.206ProGlu: 2.206 ± 0.082
1.446ProPhe: 1.446 ± 0.067
1.556ProGly: 1.556 ± 0.075
0.647ProHis: 0.647 ± 0.048
3.593ProIle: 3.593 ± 0.156
2.871ProLys: 2.871 ± 0.1
2.327ProLeu: 2.327 ± 0.119
0.599ProMet: 0.599 ± 0.055
3.425ProAsn: 3.425 ± 0.164
1.108ProPro: 1.108 ± 0.109
1.008ProGln: 1.008 ± 0.08
0.829ProArg: 0.829 ± 0.063
1.869ProSer: 1.869 ± 0.091
1.721ProThr: 1.721 ± 0.099
1.745ProVal: 1.745 ± 0.08
0.234ProTrp: 0.234 ± 0.031
1.535ProTyr: 1.535 ± 0.082
0.0ProXaa: 0.0 ± 0.0
Gln
0.964GlnAla: 0.964 ± 0.055
0.472GlnCys: 0.472 ± 0.043
1.731GlnAsp: 1.731 ± 0.079
1.883GlnGlu: 1.883 ± 0.112
1.366GlnPhe: 1.366 ± 0.067
0.957GlnGly: 0.957 ± 0.068
0.516GlnHis: 0.516 ± 0.041
3.015GlnIle: 3.015 ± 0.119
2.654GlnLys: 2.654 ± 0.105
2.537GlnLeu: 2.537 ± 0.099
0.661GlnMet: 0.661 ± 0.047
3.259GlnAsn: 3.259 ± 0.159
1.039GlnPro: 1.039 ± 0.092
1.246GlnGln: 1.246 ± 0.142
0.902GlnArg: 0.902 ± 0.092
1.869GlnSer: 1.869 ± 0.105
1.373GlnThr: 1.373 ± 0.078
1.583GlnVal: 1.583 ± 0.112
0.21GlnTrp: 0.21 ± 0.027
1.804GlnTyr: 1.804 ± 0.079
0.0GlnXaa: 0.0 ± 0.0
Arg
1.067ArgAla: 1.067 ± 0.078
0.623ArgCys: 0.623 ± 0.046
2.031ArgAsp: 2.031 ± 0.093
1.907ArgGlu: 1.907 ± 0.08
1.411ArgPhe: 1.411 ± 0.075
1.404ArgGly: 1.404 ± 0.079
0.561ArgHis: 0.561 ± 0.039
2.826ArgIle: 2.826 ± 0.12
2.581ArgLys: 2.581 ± 0.11
2.495ArgLeu: 2.495 ± 0.095
0.564ArgMet: 0.564 ± 0.041
2.898ArgAsn: 2.898 ± 0.157
0.922ArgPro: 0.922 ± 0.057
1.095ArgGln: 1.095 ± 0.065
1.263ArgArg: 1.263 ± 0.089
1.865ArgSer: 1.865 ± 0.118
1.415ArgThr: 1.415 ± 0.065
1.329ArgVal: 1.329 ± 0.083
0.303ArgTrp: 0.303 ± 0.037
1.79ArgTyr: 1.79 ± 0.089
0.0ArgXaa: 0.0 ± 0.0
Ser
1.835SerAla: 1.835 ± 0.095
1.27SerCys: 1.27 ± 0.078
4.667SerAsp: 4.667 ± 0.134
4.223SerGlu: 4.223 ± 0.192
2.716SerPhe: 2.716 ± 0.102
3.941SerGly: 3.941 ± 0.23
1.125SerHis: 1.125 ± 0.064
5.927SerIle: 5.927 ± 0.163
6.175SerLys: 6.175 ± 0.219
4.729SerLeu: 4.729 ± 0.135
1.163SerMet: 1.163 ± 0.069
5.779SerAsn: 5.779 ± 0.199
1.804SerPro: 1.804 ± 0.1
2.237SerGln: 2.237 ± 0.107
2.365SerArg: 2.365 ± 0.104
4.65SerSer: 4.65 ± 0.181
3.098SerThr: 3.098 ± 0.11
3.118SerVal: 3.118 ± 0.122
0.496SerTrp: 0.496 ± 0.044
2.96SerTyr: 2.96 ± 0.097
0.0SerXaa: 0.0 ± 0.0
Thr
1.549ThrAla: 1.549 ± 0.088
0.926ThrCys: 0.926 ± 0.064
2.709ThrAsp: 2.709 ± 0.097
2.626ThrGlu: 2.626 ± 0.119
2.365ThrPhe: 2.365 ± 0.119
2.826ThrGly: 2.826 ± 0.17
1.439ThrHis: 1.439 ± 0.144
4.822ThrIle: 4.822 ± 0.137
4.196ThrLys: 4.196 ± 0.152
3.559ThrLeu: 3.559 ± 0.108
0.812ThrMet: 0.812 ± 0.057
4.671ThrAsn: 4.671 ± 0.169
1.883ThrPro: 1.883 ± 0.102
1.483ThrGln: 1.483 ± 0.084
1.755ThrArg: 1.755 ± 0.079
3.335ThrSer: 3.335 ± 0.122
2.74ThrThr: 2.74 ± 0.157
1.986ThrVal: 1.986 ± 0.107
0.392ThrTrp: 0.392 ± 0.036
2.468ThrTyr: 2.468 ± 0.098
0.0ThrXaa: 0.0 ± 0.0
Val
1.428ValAla: 1.428 ± 0.083
0.799ValCys: 0.799 ± 0.053
2.815ValAsp: 2.815 ± 0.096
2.881ValGlu: 2.881 ± 0.105
1.958ValPhe: 1.958 ± 0.097
1.711ValGly: 1.711 ± 0.082
0.799ValHis: 0.799 ± 0.054
3.934ValIle: 3.934 ± 0.132
4.471ValLys: 4.471 ± 0.157
3.487ValLeu: 3.487 ± 0.139
0.795ValMet: 0.795 ± 0.059
3.741ValAsn: 3.741 ± 0.137
1.721ValPro: 1.721 ± 0.1
1.27ValGln: 1.27 ± 0.079
1.384ValArg: 1.384 ± 0.071
2.84ValSer: 2.84 ± 0.109
2.702ValThr: 2.702 ± 0.138
2.585ValVal: 2.585 ± 0.114
0.32ValTrp: 0.32 ± 0.039
2.323ValTyr: 2.323 ± 0.095
0.0ValXaa: 0.0 ± 0.0
Trp
0.482TrpAla: 0.482 ± 0.05
0.207TrpCys: 0.207 ± 0.031
0.478TrpAsp: 0.478 ± 0.043
0.33TrpGlu: 0.33 ± 0.037
0.368TrpPhe: 0.368 ± 0.037
0.299TrpGly: 0.299 ± 0.036
0.114TrpHis: 0.114 ± 0.022
0.857TrpIle: 0.857 ± 0.057
0.805TrpLys: 0.805 ± 0.058
0.671TrpLeu: 0.671 ± 0.062
0.193TrpMet: 0.193 ± 0.027
0.733TrpAsn: 0.733 ± 0.055
0.158TrpPro: 0.158 ± 0.023
0.165TrpGln: 0.165 ± 0.024
0.265TrpArg: 0.265 ± 0.033
0.554TrpSer: 0.554 ± 0.05
0.406TrpThr: 0.406 ± 0.045
0.341TrpVal: 0.341 ± 0.04
0.33TrpTrp: 0.33 ± 0.091
0.43TrpTyr: 0.43 ± 0.04
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.773TyrAla: 1.773 ± 0.084
1.084TyrCys: 1.084 ± 0.065
3.927TyrAsp: 3.927 ± 0.124
2.778TyrGlu: 2.778 ± 0.102
3.576TyrPhe: 3.576 ± 0.134
2.85TyrGly: 2.85 ± 0.099
1.425TyrHis: 1.425 ± 0.079
5.252TyrIle: 5.252 ± 0.157
4.464TyrLys: 4.464 ± 0.154
6.739TyrLeu: 6.739 ± 0.197
1.15TyrMet: 1.15 ± 0.061
5.115TyrAsn: 5.115 ± 0.154
1.838TyrPro: 1.838 ± 0.095
1.841TyrGln: 1.841 ± 0.074
1.776TyrArg: 1.776 ± 0.093
3.387TyrSer: 3.387 ± 0.123
2.599TyrThr: 2.599 ± 0.094
2.643TyrVal: 2.643 ± 0.105
0.416TyrTrp: 0.416 ± 0.036
3.693TyrTyr: 3.693 ± 0.147
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 894 proteins (290542 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski