Streptococcus phage Javan355

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A8Y2|A0A4D6A8Y2_9CAUD Hyaluronidase OS=Streptococcus phage Javan355 OX=2548123 GN=Javan355_0006 PE=4 SV=1
MM1 pKa = 8.14KK2 pKa = 10.17YY3 pKa = 9.71RR4 pKa = 11.84KK5 pKa = 9.62KK6 pKa = 10.3PVVIEE11 pKa = 3.77AVQFVDD17 pKa = 3.33TEE19 pKa = 4.01EE20 pKa = 6.25AIDD23 pKa = 3.84EE24 pKa = 4.5LCDD27 pKa = 4.36FGLDD31 pKa = 3.67PVRR34 pKa = 11.84IDD36 pKa = 4.13YY37 pKa = 11.34ADD39 pKa = 3.8LSNPLLKK46 pKa = 10.11IEE48 pKa = 4.17TLEE51 pKa = 4.01GLMIATEE58 pKa = 3.92GDD60 pKa = 4.05YY61 pKa = 10.49IIKK64 pKa = 10.13GVQGEE69 pKa = 4.61FYY71 pKa = 9.9PCKK74 pKa = 9.68PDD76 pKa = 3.79IFAEE80 pKa = 4.65TYY82 pKa = 9.49EE83 pKa = 4.31KK84 pKa = 10.49TEE86 pKa = 3.98EE87 pKa = 3.93

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A918|A0A4D6A918_9CAUD Peptidase_M78 domain-containing protein OS=Streptococcus phage Javan355 OX=2548123 GN=Javan355_0051 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.88LTFCDD7 pKa = 4.89LLSTMYY13 pKa = 10.9APAPCEE19 pKa = 3.66FPYY22 pKa = 10.76LVVMVLLYY30 pKa = 10.81CSLIEE35 pKa = 4.19VACWPLFEE43 pKa = 5.34RR44 pKa = 11.84GKK46 pKa = 8.6TLLVHH51 pKa = 6.77SPLRR55 pKa = 11.84WPIMSTRR62 pKa = 11.84SLFWGTPKK70 pKa = 10.49FLLLIFSHH78 pKa = 5.8STLYY82 pKa = 10.43PNSSKK87 pKa = 10.86APRR90 pKa = 11.84MVSNVAPPSWLRR102 pKa = 11.84SPLTFSRR109 pKa = 11.84NRR111 pKa = 11.84YY112 pKa = 8.66LGLRR116 pKa = 11.84IDD118 pKa = 4.01ANLAISKK125 pKa = 10.0KK126 pKa = 9.37RR127 pKa = 11.84LPRR130 pKa = 11.84VSSKK134 pKa = 10.52PRR136 pKa = 11.84LFPAMLKK143 pKa = 9.8AWHH146 pKa = 6.64GNPPQIMSTRR156 pKa = 11.84PIPRR160 pKa = 11.84INSSDD165 pKa = 3.71TAVMSCNSISPFVLCIALL183 pKa = 3.74

Molecular weight: 20.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11819

37

1044

223.0

25.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.98 ± 0.358

0.609 ± 0.098

6.422 ± 0.255

7.826 ± 0.403

3.782 ± 0.227

6.278 ± 0.432

1.574 ± 0.16

6.87 ± 0.35

8.52 ± 0.369

8.452 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.178

5.33 ± 0.228

3.173 ± 0.279

4.417 ± 0.262

4.324 ± 0.27

6.033 ± 0.336

5.533 ± 0.268

6.033 ± 0.334

1.405 ± 0.159

3.926 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski