Amino acid dipepetide frequency for Cyanophage BHS3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.826AlaAla: 4.826 ± 1.443
0.0AlaCys: 0.0 ± 0.0
5.792AlaAsp: 5.792 ± 1.105
4.505AlaGlu: 4.505 ± 1.068
4.183AlaPhe: 4.183 ± 1.046
4.826AlaGly: 4.826 ± 2.004
0.322AlaHis: 0.322 ± 0.299
5.47AlaIle: 5.47 ± 1.503
2.252AlaLys: 2.252 ± 0.98
7.722AlaLeu: 7.722 ± 1.487
1.609AlaMet: 1.609 ± 0.49
4.826AlaAsn: 4.826 ± 1.271
1.931AlaPro: 1.931 ± 0.708
2.896AlaGln: 2.896 ± 0.807
6.113AlaArg: 6.113 ± 2.19
6.435AlaSer: 6.435 ± 1.657
6.435AlaThr: 6.435 ± 1.325
4.505AlaVal: 4.505 ± 0.839
1.931AlaTrp: 1.931 ± 0.342
1.609AlaTyr: 1.609 ± 0.985
0.0AlaXaa: 0.0 ± 0.0
Cys
0.322CysAla: 0.322 ± 0.343
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
0.644CysGly: 0.644 ± 0.42
0.322CysHis: 0.322 ± 0.261
0.644CysIle: 0.644 ± 0.394
0.322CysLys: 0.322 ± 0.343
1.609CysLeu: 1.609 ± 0.618
0.0CysMet: 0.0 ± 0.0
0.322CysAsn: 0.322 ± 0.407
0.0CysPro: 0.0 ± 0.0
0.322CysGln: 0.322 ± 0.261
0.0CysArg: 0.0 ± 0.0
0.322CysSer: 0.322 ± 0.299
0.644CysThr: 0.644 ± 0.377
0.322CysVal: 0.322 ± 0.407
0.644CysTrp: 0.644 ± 0.416
0.644CysTyr: 0.644 ± 0.291
0.0CysXaa: 0.0 ± 0.0
Asp
5.792AspAla: 5.792 ± 1.235
0.644AspCys: 0.644 ± 0.338
5.792AspAsp: 5.792 ± 1.663
5.148AspGlu: 5.148 ± 1.664
1.609AspPhe: 1.609 ± 0.484
5.47AspGly: 5.47 ± 1.174
0.965AspHis: 0.965 ± 0.379
4.183AspIle: 4.183 ± 1.239
2.252AspLys: 2.252 ± 0.94
5.148AspLeu: 5.148 ± 1.553
0.644AspMet: 0.644 ± 0.792
2.896AspAsn: 2.896 ± 1.018
2.574AspPro: 2.574 ± 0.905
0.644AspGln: 0.644 ± 0.405
3.539AspArg: 3.539 ± 0.808
2.252AspSer: 2.252 ± 0.738
4.826AspThr: 4.826 ± 1.159
5.47AspVal: 5.47 ± 1.76
0.965AspTrp: 0.965 ± 0.385
0.644AspTyr: 0.644 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
5.148GluAla: 5.148 ± 1.342
0.644GluCys: 0.644 ± 0.374
2.896GluAsp: 2.896 ± 0.938
4.505GluGlu: 4.505 ± 1.993
2.252GluPhe: 2.252 ± 0.744
0.965GluGly: 0.965 ± 0.692
0.965GluHis: 0.965 ± 0.462
1.931GluIle: 1.931 ± 0.856
2.896GluLys: 2.896 ± 0.938
4.505GluLeu: 4.505 ± 1.393
0.644GluMet: 0.644 ± 0.338
1.609GluAsn: 1.609 ± 0.481
3.218GluPro: 3.218 ± 0.714
4.826GluGln: 4.826 ± 2.455
4.183GluArg: 4.183 ± 1.38
1.931GluSer: 1.931 ± 0.605
3.218GluThr: 3.218 ± 1.154
3.218GluVal: 3.218 ± 1.538
1.287GluTrp: 1.287 ± 0.664
2.574GluTyr: 2.574 ± 0.739
0.0GluXaa: 0.0 ± 0.0
Phe
1.287PheAla: 1.287 ± 0.569
0.322PheCys: 0.322 ± 0.24
4.505PheAsp: 4.505 ± 1.004
3.218PheGlu: 3.218 ± 1.235
1.931PhePhe: 1.931 ± 0.586
2.574PheGly: 2.574 ± 0.516
0.644PheHis: 0.644 ± 0.338
3.539PheIle: 3.539 ± 0.805
1.287PheLys: 1.287 ± 0.571
1.931PheLeu: 1.931 ± 0.415
1.287PheMet: 1.287 ± 0.726
2.252PheAsn: 2.252 ± 1.035
2.252PhePro: 2.252 ± 0.555
1.287PheGln: 1.287 ± 0.467
1.609PheArg: 1.609 ± 0.516
1.931PheSer: 1.931 ± 0.916
4.505PheThr: 4.505 ± 0.983
3.861PheVal: 3.861 ± 1.112
1.287PheTrp: 1.287 ± 0.639
0.965PheTyr: 0.965 ± 0.351
0.0PheXaa: 0.0 ± 0.0
Gly
4.505GlyAla: 4.505 ± 1.073
0.644GlyCys: 0.644 ± 0.377
5.148GlyAsp: 5.148 ± 1.128
3.539GlyGlu: 3.539 ± 0.803
2.574GlyPhe: 2.574 ± 0.53
8.366GlyGly: 8.366 ± 1.572
1.287GlyHis: 1.287 ± 0.687
3.539GlyIle: 3.539 ± 0.64
1.609GlyLys: 1.609 ± 0.673
4.505GlyLeu: 4.505 ± 0.647
1.287GlyMet: 1.287 ± 0.576
4.183GlyAsn: 4.183 ± 1.34
0.0GlyPro: 0.0 ± 0.0
2.252GlyGln: 2.252 ± 0.92
3.539GlyArg: 3.539 ± 0.458
5.148GlySer: 5.148 ± 1.013
6.435GlyThr: 6.435 ± 1.961
5.148GlyVal: 5.148 ± 0.876
0.0GlyTrp: 0.0 ± 0.0
4.183GlyTyr: 4.183 ± 1.176
0.0GlyXaa: 0.0 ± 0.0
His
0.322HisAla: 0.322 ± 0.365
0.0HisCys: 0.0 ± 0.0
1.287HisAsp: 1.287 ± 0.864
0.322HisGlu: 0.322 ± 0.261
0.644HisPhe: 0.644 ± 0.487
0.965HisGly: 0.965 ± 0.666
0.0HisHis: 0.0 ± 0.0
1.287HisIle: 1.287 ± 0.973
0.644HisLys: 0.644 ± 0.338
1.609HisLeu: 1.609 ± 0.871
0.0HisMet: 0.0 ± 0.0
0.322HisAsn: 0.322 ± 0.261
0.322HisPro: 0.322 ± 0.299
0.644HisGln: 0.644 ± 0.291
0.644HisArg: 0.644 ± 0.405
0.322HisSer: 0.322 ± 0.261
0.644HisThr: 0.644 ± 0.405
1.609HisVal: 1.609 ± 0.806
0.322HisTrp: 0.322 ± 0.261
0.644HisTyr: 0.644 ± 0.597
0.0HisXaa: 0.0 ± 0.0
Ile
2.574IleAla: 2.574 ± 0.582
0.644IleCys: 0.644 ± 0.42
5.47IleAsp: 5.47 ± 1.204
3.218IleGlu: 3.218 ± 0.583
1.287IlePhe: 1.287 ± 0.78
3.218IleGly: 3.218 ± 0.774
0.965IleHis: 0.965 ± 0.515
1.609IleIle: 1.609 ± 0.914
2.574IleLys: 2.574 ± 1.203
3.218IleLeu: 3.218 ± 1.031
0.965IleMet: 0.965 ± 0.483
2.252IleAsn: 2.252 ± 0.631
3.539IlePro: 3.539 ± 1.151
2.574IleGln: 2.574 ± 0.698
3.861IleArg: 3.861 ± 1.101
3.218IleSer: 3.218 ± 0.915
3.218IleThr: 3.218 ± 0.921
4.505IleVal: 4.505 ± 1.25
0.965IleTrp: 0.965 ± 0.515
1.609IleTyr: 1.609 ± 0.829
0.0IleXaa: 0.0 ± 0.0
Lys
5.148LysAla: 5.148 ± 0.675
0.0LysCys: 0.0 ± 0.0
0.322LysAsp: 0.322 ± 0.261
2.574LysGlu: 2.574 ± 1.272
0.644LysPhe: 0.644 ± 0.374
1.287LysGly: 1.287 ± 0.39
0.0LysHis: 0.0 ± 0.0
1.287LysIle: 1.287 ± 0.639
1.931LysLys: 1.931 ± 0.683
2.574LysLeu: 2.574 ± 0.766
0.0LysMet: 0.0 ± 0.0
1.609LysAsn: 1.609 ± 0.465
2.896LysPro: 2.896 ± 0.633
2.574LysGln: 2.574 ± 0.955
4.826LysArg: 4.826 ± 1.668
0.644LysSer: 0.644 ± 0.597
2.252LysThr: 2.252 ± 0.472
4.183LysVal: 4.183 ± 0.982
0.322LysTrp: 0.322 ± 0.299
0.644LysTyr: 0.644 ± 0.417
0.0LysXaa: 0.0 ± 0.0
Leu
9.331LeuAla: 9.331 ± 1.574
1.287LeuCys: 1.287 ± 0.49
4.505LeuAsp: 4.505 ± 0.799
3.539LeuGlu: 3.539 ± 1.1
2.896LeuPhe: 2.896 ± 1.116
4.183LeuGly: 4.183 ± 0.929
0.644LeuHis: 0.644 ± 0.291
2.574LeuIle: 2.574 ± 0.551
3.218LeuLys: 3.218 ± 1.6
6.113LeuLeu: 6.113 ± 1.349
3.539LeuMet: 3.539 ± 1.038
2.896LeuAsn: 2.896 ± 0.659
2.574LeuPro: 2.574 ± 0.568
3.218LeuGln: 3.218 ± 0.731
5.148LeuArg: 5.148 ± 1.249
4.183LeuSer: 4.183 ± 0.687
5.148LeuThr: 5.148 ± 1.438
5.792LeuVal: 5.792 ± 0.85
0.965LeuTrp: 0.965 ± 0.624
4.505LeuTyr: 4.505 ± 1.116
0.0LeuXaa: 0.0 ± 0.0
Met
1.931MetAla: 1.931 ± 1.309
0.644MetCys: 0.644 ± 0.42
0.644MetAsp: 0.644 ± 0.416
1.609MetGlu: 1.609 ± 0.485
0.322MetPhe: 0.322 ± 0.261
0.965MetGly: 0.965 ± 0.503
0.644MetHis: 0.644 ± 0.597
0.644MetIle: 0.644 ± 0.416
1.931MetLys: 1.931 ± 0.777
1.931MetLeu: 1.931 ± 1.374
0.965MetMet: 0.965 ± 0.406
0.644MetAsn: 0.644 ± 0.417
1.931MetPro: 1.931 ± 0.873
0.322MetGln: 0.322 ± 0.351
0.644MetArg: 0.644 ± 0.486
1.931MetSer: 1.931 ± 0.572
3.539MetThr: 3.539 ± 0.805
0.644MetVal: 0.644 ± 0.303
0.322MetTrp: 0.322 ± 0.261
0.965MetTyr: 0.965 ± 0.436
0.0MetXaa: 0.0 ± 0.0
Asn
6.435AsnAla: 6.435 ± 2.013
0.0AsnCys: 0.0 ± 0.0
3.218AsnAsp: 3.218 ± 0.782
2.574AsnGlu: 2.574 ± 0.922
1.931AsnPhe: 1.931 ± 0.428
4.183AsnGly: 4.183 ± 0.703
0.0AsnHis: 0.0 ± 0.0
1.609AsnIle: 1.609 ± 0.607
1.287AsnLys: 1.287 ± 0.572
2.574AsnLeu: 2.574 ± 0.711
1.609AsnMet: 1.609 ± 0.711
3.218AsnAsn: 3.218 ± 0.873
3.861AsnPro: 3.861 ± 0.522
1.609AsnGln: 1.609 ± 0.617
3.539AsnArg: 3.539 ± 1.006
3.539AsnSer: 3.539 ± 0.962
4.505AsnThr: 4.505 ± 1.735
6.435AsnVal: 6.435 ± 0.995
0.322AsnTrp: 0.322 ± 0.261
1.287AsnTyr: 1.287 ± 0.732
0.0AsnXaa: 0.0 ± 0.0
Pro
2.252ProAla: 2.252 ± 0.739
0.322ProCys: 0.322 ± 0.24
2.574ProAsp: 2.574 ± 0.666
3.218ProGlu: 3.218 ± 0.859
1.287ProPhe: 1.287 ± 0.68
3.539ProGly: 3.539 ± 1.338
0.322ProHis: 0.322 ± 0.261
1.931ProIle: 1.931 ± 0.888
1.287ProLys: 1.287 ± 0.639
4.826ProLeu: 4.826 ± 1.463
1.287ProMet: 1.287 ± 0.529
5.148ProAsn: 5.148 ± 1.287
3.861ProPro: 3.861 ± 1.347
1.287ProGln: 1.287 ± 0.39
2.896ProArg: 2.896 ± 1.697
3.218ProSer: 3.218 ± 0.539
3.861ProThr: 3.861 ± 0.496
3.861ProVal: 3.861 ± 0.947
0.644ProTrp: 0.644 ± 0.374
1.931ProTyr: 1.931 ± 0.57
0.0ProXaa: 0.0 ± 0.0
Gln
4.505GlnAla: 4.505 ± 1.482
0.0GlnCys: 0.0 ± 0.0
0.965GlnAsp: 0.965 ± 0.594
1.931GlnGlu: 1.931 ± 0.898
0.965GlnPhe: 0.965 ± 0.546
1.609GlnGly: 1.609 ± 0.588
0.965GlnHis: 0.965 ± 0.541
1.931GlnIle: 1.931 ± 0.574
0.965GlnLys: 0.965 ± 0.379
1.609GlnLeu: 1.609 ± 0.661
0.965GlnMet: 0.965 ± 0.436
2.574GlnAsn: 2.574 ± 0.663
2.574GlnPro: 2.574 ± 0.856
11.261GlnGln: 11.261 ± 5.363
4.183GlnArg: 4.183 ± 1.465
1.287GlnSer: 1.287 ± 0.439
3.218GlnThr: 3.218 ± 0.629
1.931GlnVal: 1.931 ± 0.855
0.644GlnTrp: 0.644 ± 0.303
2.896GlnTyr: 2.896 ± 0.818
0.0GlnXaa: 0.0 ± 0.0
Arg
3.861ArgAla: 3.861 ± 1.885
0.322ArgCys: 0.322 ± 0.396
4.183ArgAsp: 4.183 ± 0.857
3.861ArgGlu: 3.861 ± 1.468
5.148ArgPhe: 5.148 ± 1.084
3.218ArgGly: 3.218 ± 0.655
1.609ArgHis: 1.609 ± 0.504
5.148ArgIle: 5.148 ± 1.929
3.218ArgLys: 3.218 ± 1.301
5.47ArgLeu: 5.47 ± 0.701
3.218ArgMet: 3.218 ± 0.69
3.861ArgAsn: 3.861 ± 0.946
2.574ArgPro: 2.574 ± 0.668
1.287ArgGln: 1.287 ± 0.376
8.044ArgArg: 8.044 ± 2.344
2.252ArgSer: 2.252 ± 0.437
3.539ArgThr: 3.539 ± 0.831
3.861ArgVal: 3.861 ± 0.759
0.0ArgTrp: 0.0 ± 0.0
3.218ArgTyr: 3.218 ± 0.639
0.0ArgXaa: 0.0 ± 0.0
Ser
3.861SerAla: 3.861 ± 1.196
0.322SerCys: 0.322 ± 0.396
3.861SerAsp: 3.861 ± 0.675
1.931SerGlu: 1.931 ± 0.633
4.826SerPhe: 4.826 ± 1.671
2.896SerGly: 2.896 ± 1.327
0.644SerHis: 0.644 ± 0.374
2.896SerIle: 2.896 ± 1.089
0.644SerLys: 0.644 ± 0.377
4.505SerLeu: 4.505 ± 1.057
1.287SerMet: 1.287 ± 0.619
4.183SerAsn: 4.183 ± 1.298
2.896SerPro: 2.896 ± 0.591
2.252SerGln: 2.252 ± 0.421
3.861SerArg: 3.861 ± 1.538
7.722SerSer: 7.722 ± 3.046
5.148SerThr: 5.148 ± 1.422
6.113SerVal: 6.113 ± 1.001
0.965SerTrp: 0.965 ± 0.457
2.574SerTyr: 2.574 ± 0.73
0.0SerXaa: 0.0 ± 0.0
Thr
7.4ThrAla: 7.4 ± 1.261
0.644ThrCys: 0.644 ± 0.303
2.896ThrAsp: 2.896 ± 0.659
1.931ThrGlu: 1.931 ± 0.576
6.435ThrPhe: 6.435 ± 1.099
6.757ThrGly: 6.757 ± 1.324
0.644ThrHis: 0.644 ± 0.486
4.183ThrIle: 4.183 ± 1.001
3.218ThrLys: 3.218 ± 0.917
8.044ThrLeu: 8.044 ± 1.395
0.644ThrMet: 0.644 ± 0.416
4.505ThrAsn: 4.505 ± 1.637
5.792ThrPro: 5.792 ± 1.096
1.931ThrGln: 1.931 ± 0.909
3.218ThrArg: 3.218 ± 0.956
9.331ThrSer: 9.331 ± 2.138
6.757ThrThr: 6.757 ± 3.17
4.183ThrVal: 4.183 ± 1.273
0.644ThrTrp: 0.644 ± 0.291
0.965ThrTyr: 0.965 ± 0.497
0.0ThrXaa: 0.0 ± 0.0
Val
5.148ValAla: 5.148 ± 1.177
0.322ValCys: 0.322 ± 0.407
4.826ValAsp: 4.826 ± 0.858
3.539ValGlu: 3.539 ± 1.285
2.252ValPhe: 2.252 ± 0.631
8.044ValGly: 8.044 ± 1.505
0.965ValHis: 0.965 ± 0.541
3.861ValIle: 3.861 ± 1.249
2.252ValLys: 2.252 ± 0.8
5.148ValLeu: 5.148 ± 1.207
1.609ValMet: 1.609 ± 0.442
3.218ValAsn: 3.218 ± 1.582
5.792ValPro: 5.792 ± 1.385
1.931ValGln: 1.931 ± 0.342
4.826ValArg: 4.826 ± 1.253
5.148ValSer: 5.148 ± 1.893
6.757ValThr: 6.757 ± 1.301
7.722ValVal: 7.722 ± 1.481
0.644ValTrp: 0.644 ± 0.303
3.218ValTyr: 3.218 ± 1.004
0.0ValXaa: 0.0 ± 0.0
Trp
0.644TrpAla: 0.644 ± 0.48
0.322TrpCys: 0.322 ± 0.261
0.0TrpAsp: 0.0 ± 0.0
0.0TrpGlu: 0.0 ± 0.0
0.644TrpPhe: 0.644 ± 0.486
0.965TrpGly: 0.965 ± 0.464
0.322TrpHis: 0.322 ± 0.299
1.287TrpIle: 1.287 ± 0.551
0.322TrpLys: 0.322 ± 0.299
0.644TrpLeu: 0.644 ± 0.374
0.965TrpMet: 0.965 ± 0.545
0.965TrpAsn: 0.965 ± 0.379
0.0TrpPro: 0.0 ± 0.0
1.287TrpGln: 1.287 ± 0.581
0.965TrpArg: 0.965 ± 0.525
1.287TrpSer: 1.287 ± 0.489
2.252TrpThr: 2.252 ± 1.032
0.644TrpVal: 0.644 ± 0.303
0.322TrpTrp: 0.322 ± 0.24
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.896TyrAla: 2.896 ± 0.828
0.0TyrCys: 0.0 ± 0.0
2.574TyrAsp: 2.574 ± 0.857
2.252TyrGlu: 2.252 ± 0.65
0.644TyrPhe: 0.644 ± 0.486
3.539TyrGly: 3.539 ± 0.668
0.322TyrHis: 0.322 ± 0.365
1.931TyrIle: 1.931 ± 0.831
1.609TyrLys: 1.609 ± 0.688
2.574TyrLeu: 2.574 ± 1.064
0.322TyrMet: 0.322 ± 0.24
2.252TyrAsn: 2.252 ± 0.504
0.965TyrPro: 0.965 ± 0.351
2.574TyrGln: 2.574 ± 0.788
2.252TyrArg: 2.252 ± 0.471
1.287TyrSer: 1.287 ± 0.447
3.218TyrThr: 3.218 ± 0.787
3.218TyrVal: 3.218 ± 1.004
0.644TyrTrp: 0.644 ± 0.486
2.574TyrTyr: 2.574 ± 0.642
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 9 proteins (3109 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski